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Hermanson G.T. — Bioconjugate Techniques
Hermanson G.T. — Bioconjugate Techniques

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Название: Bioconjugate Techniques

Автор: Hermanson G.T.

Аннотация:

This is a well-organized and well-written book; it is readable and comprehensible to the novice, but its content and stylistic approach seem sufficiently sophisticated to appeal to active, knowledgeable workers in the field. It is an impressive compilation of useful theoretical and practical information that is not readily available elsewhere in a single volume.


Язык: en

Рубрика: Технология/

Статус предметного указателя: Готов указатель с номерами страниц

ed2k: ed2k stats

Год издания: 1996

Количество страниц: 785

Добавлена в каталог: 15.02.2007

Операции: Положить на полку | Скопировать ссылку для форума | Скопировать ID
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Предметный указатель
$17\beta$-Estradiol, conjugation to cBSA using the Man-nich reaction      453
$AgNO_3$      69
$F(ab')_2$ fragments      236 459 482 486 501
$F(ab')_2$ fragments, preparation of      478 479
$F(ab')_2$ fragments, reduction of      482
$F(ab')_2$ fragments, reduction of, with 2-mercaptoethylamine      82 463
$FADH_2$      634
$I^{-}$      401
$NAD^+$      497
$\alpha 2$-Macroglobulin in cytotoxic conjugates      495
$\alpha 2$-Macroglobulin, SPDP conjugation to toxins      505
$\alpha$-Carboxyl group      4
$\alpha$-Crystalline      205
$\alpha$-Haloacetamides      131 147
$\alpha$-Haloacetates, relative reactivity of      99
$\alpha$-Helix      6
$\alpha$-Hydroxy aldehydes      32
$\alpha$-Hydroxy ketones      32
$\alpha$-Lactalbumin      205
$\beta$ emitters      400
$\beta$-(p-Aminophenyl)ethyl-amine      31 447
$\beta$-Alanine      623
$\beta$-D-2-Deoxyribose      29 31 50
$\beta$-D-Galactosidase      210 236 241 461 576
$\beta$-D-Galactosidase, conjugation with avidin or streptavidin      575 577
$\beta$-D-Galactosidase, general description and use      633
$\beta$-D-Galactosidase, sulfhydryl content of      460
$\beta$-D-Galactoside      633
$\beta$-D-Galactoside galactohydrolase      see “$\beta$-galactosidase”
$\beta$-D-Glucose oxygen 1-oxidoreductase      see “Glucose oxidase”
$\beta$-D-ribose      27 29 41
$\beta$-Endorphin      196 198
$\beta$-Mercaptoethanol      see “2-Mercaptoethanol”
$\delta$-D-Gluconolactone      634
$\gamma$ emitters      400
$\mathrm S_2O_3^{-2}}$      see “Thiosulfate”
$\mathrm{BS^3}$, general description and use      196
$\mathrm{CO_2}$      138 143 156 158 184 185 202 611 615
$\mathrm{HAuCl_4}$      see “Chloroauric acid”
$\mathrm{H_2OI^+}$      401
$\mathrm{H_2O_2}$      410 (see also “Hydrogen peroxide”
$\mathrm{I_2}$      401 410
$\mathrm{MgCl_2}$      641 643
$\mathrm{M_2C_2H}$, general description and use      250
$\mathrm{NaBH_4}$      see “Sodium borohydride”
$\mathrm{Na_2SO_3}$      see “Sodium sulfite”
$\mathrm{Na_2S_2O_4}$      see “Sodium dithionite”
$\mathrm{Na_2S_4O_6}$      see “Sodium tetrathionate”
$\mathrm{^{10}B}$      495
$\mathrm{^{11}B}$      495
$\mathrm{^{14}C}$      400
$\omega$-Conotoxin      322
$\varepsilon$-N-Biotinyl-L-lysine      see “Biocytin”
$^{125}I$      214 400 403 404 407 408 410 488 490
$^{125}I$, avidin labeled with      574
$^{125}I$, comparison to other radioisotopes      401
$^{125}I$, effect on fluorophore emission      400
$^{125}I$, modification of heterobifunctional cross-linkers with      416
$^{125}I$, modification of SHPP with      412
$^{125}I$, protein modification with      401
$^{131}I$      400 403 408
$^{131}I$, effect on fluorophore emission      400
1,2-Dimyristoyl      536
1,2:3,4-Diepoxybutane      221
1,2:5,6-Diepoxyhexane      221
1,3,4,6-Tetrachloro-3a,6a-diphenylglycouril      see “IODO-GEN”
1,3-Diaminopropane      101 645
1,3-Diaminourea      see “Car-bohydrazide”
1,3-Dibromoacetone      226
1,4,7,10-Tetraazacyclododecane-N,N',N",N'"-tetraacetic acid      see “DOTA”
1,4,7-Triazacyclononane-N,N',N"-triacetic acid      see “NOTA”
1,4,8,11 -Tetraazacyclotetradecane-N,N',N",N'"-tetraacetic acid      see “TETA”
1,4-butanediol diglycidyl ether      33 221
1,4-butanediol diglycidyl ether, general description and use      221
1,4-butanediol diglycidyl ether, modification of dextran with      627
1,4-Di-[3’-(2’-pyridyldithio)-propionamidol butane      see “DPDPB”
1,4-Phenylene diisothiocyanate      see “PDITC”
1,6-Diaminohexane      101 110 645 648
1,6-Diaminohexane in AMCA-HPDP      335
1,6-Diaminohexane in biotin-HPDP      386
1,6-Diaminohexane in iodoacetyl-LC-biotin      388
1-Biotinamido-4-[4’-(maleimido-methyl)cyclohexane-carboxamido]butane      see “Biotin-BMCC”
1-Cyclohexyl-3-(2-morpho-linoethyl)carbodiimide      see “CMC”
1-Ethyl-3-(3-dimethylamino-propyl)carbodiimide hydrochloride      see “EDC”
2,2’-Dipyridyl disulfide      133 653
2,2’-Dithiobis(ethylamine)      see “Cystamine”
2,3-Dihydroxy-l,4-dithiolbutane      see “DTT DTE”
2,3-Dimercaptopropanol      77
2,4,6-Trinitrobenzenesulfonate      see “TNBS”
2,4-Dinitrofiuorobenzene      143 150 372
2-(N-Morpholino)ethane sulfonic acid      see “MES”
2-Acetamido-4-mercaptobutyric acid      see “N-acetyl homocysteine thiolactone”
2-Acetamido-4-mercaptobutyric acid hydrazide      see “AMBH”
2-Amino-2-methyl- 1propanol      632
2-Imino-imidazole      380
2-Iminothiolane      151 370 441 465 482 517 520
2-Iminothiolane, general description and use      57
2-Iminothiolane, thiolation of antibodies      461 465
2-Iminothiolane, thiolation of avidin or streptavidin      588
2-Iminothiolane, thiolation of gelonin      499 500 510
2-Iminothiolane, thiolation of PAPs      499
2-Iminothiolane, thiolation of phycobiliproteins      364
2-Iminothiolane, thiolation of toxins      504 509 521
2-Iminothiolane, use with 4,4'-dipyridyl disulfide      59 134
2-Mercaptoethanol      77 85 130 192 240 351 352 359 387 410 439 442 641 643
2-Mercaptoethanol for quenching EDC reactions      176 438
2-Mercaptoethanol for reduction of disulfides      80
2-Mercaptoethanol in 2D electrophoresis      81
2-Mercaptoethanol in gram-negative bacteria lysis buffer      80
2-Mercaptoethanol in OPA reagent      82 113
2-Mercaptoethanol in SDS sample buffer      81
2-Mercaptoethanol, effect on aryl azides      254
2-Mercaptoethanol, general description and use      80
2-Mercaptoethanol, reaction with maleimide-activated BSA      443
2-Mercaptoethylamine      73 76 77 463 651 652
2-Mercaptoethylamine as a spacer in creating iodoacetyl-activated liposomes      569
2-Mercaptoethylamine for reduction $F(ab')_2$ fragments      459 482
2-Mercaptoethylamine for reduction of antibody molecules      336 459 461 463
2-Mercaptoethylamine, general description and use      82
2-Methylmaleic anhydride      see “Citraconic anhydride”
2-Oxo-imidazole      380
2-Pyridyl disulfide      133 231 232 276 293 311 335 505 542 637 653 663
2-Pyridyl disulfide in biotinylation reagents      384
2-Pyridyl disulfide in carbonyl and sulfhydryl reactive cross-linkers      249
2-Pyridyl disulfide of AMCA-HPDP      335
2-Pyridyl disulfide of APDP      277
2-Pyridyl disulfide of biotin-HPDP      386
2-Pyridyl disulfide of DPDPB      209
2-Pyridyl disulfide of PDPH      252 371
2-Pyridyl disulfide of PDTP      513
2-Pyridyl disulfide of SMPT      232 511
2-Pyridyl disulfide of SPDP      231
2-Pyridyl disulfide of SPDP-dextran      628
2-Pyridyl disulfide, immobilized      501
2-Pyridyl disulfide, reactions of      151
2-Pyridyl disulfide, use in forming immunotoxins      503
2-[[Tris(hydroxymethyl)methyl]amino]ethane-sulfonic acid      see “TES”
2H-1-Benzopyran-2-one      see “Coumarin”
5 M in 1 N NaOH      582
5 M in 1 N NaOH for reducing hydrazone bonds      32 121 159 390 392
5 M in 1 N NaOH in the preparation of immunotoxins      524
5-Acetylmercaptosuccinic anhydride      see “SAMSA”
A chain      499 500 502 508
A chain of diphtheria toxin      497
A chain of plant toxins      497
A chain, cell entry of      497
A chain, cleavage from B chain      499
A chain, conjugation via disulfide exchange      503
A chain, conjugation with antibody using SMPT      511
A chain, conjugation with SPDP-activated antibodies      504
A chain, turnover rate of      497
ABC      572 575 637
ABC system      493
Abdell      199
Abdella      295
ABH, general description and use      282
ABNP, general description and use      287
Abou-Samra      584
Abrin      497 498
Abrin, oxidation with sodium periodate      525
Abrin, structure of      497
Abrus precatorius      497
Absorption      298
Abuchowski      606 607 608 614
Acetal      34
Acetal linkages, in cleavable reagents      293
Acetamide      99 127
Acetic acid      546
Acetic anhydride      33 127 128 145
Acetic anhydride, for blocking amines      127
Acetic anhydride, reaction with mPEG in DMSO      618
Acetonitrile      586
Acetyl azide, of Cascade Blue      354
Acetylation      127 128
Acetylhydroxamate      128
Acid chlorides      140 323
Acid chlorides of NHS-chloroformate      156
Acid phosphatase      222
Acryloyl compounds, acrylic acid      149
Acryloyl compounds, acrylodan      149
Acryloyl compounds, reactions of      149
Actin      308 310 322 328 389
Active center      21 460
Active hydrogen      216 224 226 398
Active hydrogen, in the Mannich reaction      160
Active hydrogen, reaction of      161
Active hydrogen, reaction with diazonium      161
Active transport      193 497
Acyl azides, chemistry of      139
Acyl azides, in the formation of isocyanates      158
Acyl azides, transformation to isocyanates      138
Acylation      8
Acylation by anhydrides      145
Acylation by photolysis of diazopyruvates      272
Acylation of amines      90 137
Acylation of amines, blocking of      126
Acylation of hydroxyls      29
Acylation of nucleic acids      44
Acylation of pyrimidines      44 45
Acylation with citraconic anhydride      96
Acylation with glutaric anhydride      94
Acylation with maleic anhydride      94
Acylation with NHS esters      139 191
Acylation with succinic anhydride      91
Acylation, using imidoesters      203
Acyltransferase      394
Adams      216
Adaptive immunity      419
Adenine      41
Adenine, activation with bromine compounds      647
Adenine, biotin derivative of      656
Adenine, bromination of      48
Adenine, electrophilic reactivity      48
Adenine, Fischer — Dimroth rearrangement      48
Adenine, hydrogen bonding      53
Adenine, modification with diazonium compounds      658
Adenine, nucleophilic displacement reactions      47
Adenine, reaction with glutaraldehyde      49
Adenine, structure and reactivity      47
Adenylate cyclase      208 274
ADH      see “Adipic acid dihydrazide”
Adipaldehyde      215
Adipic acid      222
Adipic acid dihydrazide      121 122 123 125 590 625
Adipic acid dihydrazide, coupling to 5'-phosphate of DNA      649
Adipic acid dihydrazide, enzyme modification using      637
Adipic acid dihydrazide, general description and use      222
Adipic acid dihydrazide, modification of avidin or streptavidin with      589
Adipic acid dihydrazide, modification of phosphates      124
Adipic acid dihydrazide, reaction with aldehyde compounds      121
Adjuvant      425 429
Adolfson      206
ADP      51
ADP-ribosylation      497
Adriamycin, coupling to oxidized dextran      527
Affinity chromatography      177 220 222 382 387 394 456 607
Affinity chromatography for purification of antibody-enzyme conjugates      484
Affinity chromatography in immunotoxin preparation      517
Affinity chromatography, metal chelate      484 486
Affinity ligands      93 154
Affinsen      182
Agarose      33 36 85
Agarose, used as a carrier      421
Agglutination      552
Agre      599
Aguirre      308
Ahn      314
Aikawa      239
Aime      367
Aithal      239
Ajtai      326
Akerblom      607
Alagon      58
Alanine      5
Albrecht      597 600
Albumin      85 212 258
Alcohol dehydrogenase      286
Aldehydes      108 428
Aldehydes in the Mannich reaction      161
Aldehydes, blocking of      126 134
Aldehydes, chemistry of      141
Aldehydes, conjugation using carbonyl reactive heterobifunctionals      249
Aldehydes, coupling by reductive amination      185
Aldehydes, detection of using hydrazide compounds      120
Aldehydes, formation from hydrolyzed epoxides      220
Aldehydes, formation from oxidases      157
Aldehydes, formation from periodate oxidation of hydroxyls      156
Aldehydes, formation with SFB and SFPA      117
Aldehydes, formation, using glutaraldehyde      119
Aldehydes, formation, using oxidases      116
Aldehydes, introduction of      114
Aldehydes, modification with bis-hydrazides      121
Aldehydes, modification with diamines      108
Aldehydes, reaction of      159
Aldehydes, reaction with $M_2C_2H$      250
Aldehydes, reaction with AMBH      71
Aldehydes, reaction with ammonia or diamines      109
Aldehydes, reaction with hydrazides      222 223
Aldehydes, reaction with PDPH      252
Aldehydes, reduction to hydroxyls      141
Aldehydes, reductive amination      160
Aldol condensation      470 473
Aldolase      274
Aldonic acid      29
Aldose      27
Alexander      149
Algae      362
Aliosman      227
Alkaline phosphatase      236 243 460 484 576 634
Alkaline phosphatase, aldehyde creation with SFB      117 668
Alkaline phosphatase, antibody conjugates, purification of      486
Alkaline phosphatase, avidin-labeled      492
Alkaline phosphatase, conjugation to avidin or streptavidin      394 572 575 661
Alkaline phosphatase, conjugation to cystamine-modified DNA      663
Alkaline phosphatase, general description and use      632
Alkaline phosphatase, labeling with photobiotin      395
Alkaline phosphatase, SPDP-activated      651 664
Alkaline phosphatase, thiolation using SATA      577
Alkyl anilines      402
Alkyl glycosides      532
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