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| Hermanson G.T. — Bioconjugate Techniques |
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| Предметный указатель |
Sulforhodamine 101 354
Sulforhodamine B, hydrazide derivative of 328 (see also “Lissamine rhodamine B”)
Sulforhodamine, hydrazide derivative of 330
Sulforhodamine, sulfonyl chloride derivative of 324
SulfosuccinimidyI-4-(N-maleimidomethyl)cyclohexane-1-carboxylate see “Sulfo-SMCC”
SulfosuccinimidyI-6-(4'-azido-2'-nitrophenylamino)hexanoate see “Sulfo-SANPAH”
Sulfosuccinimidyl 2-(7-azido-4-methylcoumarin-3-acetamide)ethyl-1,3'-dithiopropionate see “SAED”
Sulfosuccinimidyl 4-(p-azidophenyl)butyrate see “Sulfo-SAPB”
Sulfosuccinimidyl 7-azido-4-methylcoumarin-3-acetate see “Sulfo-SAMCA”
Sulfosuccinimidyl(4-iodoacetyl)aminobenzoate see “Sulfo-SIAB”)
Sulfosuccinimidyl-2-(biotin-amido)ethyl-l,3-dithiopropionate see “Sulfo-NHS-SS-biotin”
Sulfosuccinimidyl-2-(m-azido-o-nitrobenzamido)-ethyl-l,3'-dithiopropionate see “SAND”
Sulfosuccinimidyl-2-(p-azidosalicylamido)ethyl-l,3'-dithiopropionate see “SASD”
Sulfosuccinimidyl-2-[6-(biotinamido)-2-(p-azidobenzamido)hexanoamido] ethyl-1,3 '-dithiopropionate see “Sulfo-SBED”
Sulfosuccinimidyl-3-(4-hydroxyphenyl)propionate see “Sulfo-SHPP”
Sulfosuccinimidyl-4-(p-maleimidophenyl)butyrate see “Sulfo-SMBP”
Sulfosuccinimidyl-6-[ -methyl--(2-pyridyldithio)toluamido]hexanoate see “Sulfo-LC-SMPT”
Sulkowski 486
Sun 361
Sundberg 33 221 626
Sunlamps 254 397 658
Sunn 59
Surfaces, polymer modification of 605
Suter 379
Sutoh 248 389
Swanson 208 239 359
Sweeley 36
Sweet clover 331
Swiss 3T3 cells 201
Sykaluk 62
Sympathetic ganglia 260
Synthetic polymers, modification with 605
Sytkowski 574
Szabo 586
Szoka 533
T-cell 208
Tacchini-Vonlanthen 597
Tadayoni 93
Tager 212
Tags, general description and use 297
Takadate 355
Takahashi 285
Talsma 533
Tanaka 322
Tanford 12
Tanke 302
TAO 279
Taq polymerase 640
Tarentino 59
Targeting, liposomal reagents 552
Tarn 428
Tartaric acid, as a cleavable, group in cross-linkers 294
Tarver 193
Tawney 209 211
Taylor 71 328 593 597
TCEP, general description and use 83
Teale 243
Technetium-99m 252 365
Terbium 368
Terminal deoxynucleotide transferase 640
Terminal transferase 641 643 670
Ternynck 220
TES 309
TETA, general description and use 367
Tetanus toxoid 422
Tetanus toxoid, use as carrier 427
Tetra-pyrrole 362
Tetramethylrhodamine 317 330
Tetramethylrhodamine, comparison to Texas Red 325
Tetramethylrhodamine, comparison with Lissamine rhodamine B 323
Tetramethylrhodamine, isothiocyanate derivative of 317
Tetramethylrhodamine, sulfhydryl-reactive derivatives 326
Tetramethylrhodamine-5-(and 6)-iodoacetamide, general description and use 326
Tetramethylrhodamine-5-(and 6)-isothiocyanate see “TRITC”
Tetramethylrhodamine-5-(and-6)-iodoacetamide 328
Tetranitromethane 110 111
Tetranitromethane in the nitration of tyrosine 447
Tetrathionate see “Sodium tetrathionate”
Texas red 302 317 325 488
Texas Red hydrazide, general description and use 330
Texas Red sulfonyl chloride 326 670
Texas Red sulfonyl chloride, coupling to diaminemodified DNA 671
Texas Red sulfonyl chloride, general description and use 324
Texas Red sulfonyl chloride, hydrazide derivative of 330
Texas red, hydrazide derivatives of 328
Thakur 370
Theis 131
Thelen 176 438
Theorell 632
Therien 428 533 549 550
Thermochemical 254
Thermoreactive 254
Thermus aquaticus 640
Thevenin 270
Thin-layer chromatography 544
Thioctic acid, immobilized 86
Thioester 94
Thioester bonds, hydroxylamine—cleavable 295
Thioether 106 209
Thioether, formation with epoxides 220
Thioether, formation with maleimides 211
Thioglycolate 77
Thioglycolic acid 85
Thiol 88
Thiolate anion 106 130 131 148 503
Thiolation 56
Thiolation at carbohydrate residues in antibodies 371
Thiolation of cytosine 46
Thiolation through disulfide reduction 76
Thiolation, using 2-iminothiolane 57 59
Thiolation, using AMBH 71
Thiolation, using cystamine 72
Thiolation, using N-acetyl homocysteine thiolactone 68
Thiolation, using PDPH 252
Thiolation, using SAMSA 70
Thiolation, using SATA 60
Thiolation, using SMPT 66
Thiolation, using SPDP 64
thiols see “Sulfhydryls”
Thionyl chloride 141
Thiosulfate 131
Thiourea linkage 138
Thomas 57 177 208
Thorpe 66 233 499 500 504 511 517
Threonine 6 36 92 94
Thrombin 313 394
Thylakoid membrane 208
Thymine 41
Thymine, cycloaddition to 46
Thymine, hydrogen bonding 53
Thymine, structure and reactivity 42
Thyroglobulin 422
Thyroglobulin, use as carrier 426 427
Thyroid gland 427
Thyroxine 427
Time-resolved fluoroimmunoassay 368 369
Tinglu 595
Titus 326
Tjerneld 607
TNB 88 151
TNB as a sulfhydryl-blocking group 133
TNB, extinction coefficient 88 132 152
TNB-thiol, reactions of 151
TNBS 94
TNBS for measuring protease activity 92 126
TNBS, determining of amines 112
TNF 196
Tokuyasu 595
Tollen’s reagent 27
Tonka beans 331
| Tonsil antigens 585
Torchilin 366 370
Tosyl chloride 33
Toxicity, of liposome-encapsulated drugs 552
Toxins 502
Toxins in the design of immunotoxins 499
Toxins, activation with SIAB 517
Toxins, activation with SPDP 504 507
Toxins, conjugation to antibodies using oxidized dextrans 527
Toxins, conjugation to antibodies using SMPT 511
Toxins, conjugation to antibody using cystamine 515
Toxins, conjugation to drugs 517
Toxins, conjugation using amine—and sulfhydryl-reactive heterobifunctionals 516
Toxins, conjugation using intact A-B types 516
Toxins, conjugation using MBS 522
Toxins, conjugation using SIAB 517
Toxins, conjugation using SMCC 519
Toxins, conjugation via disulfide exchange 503
Toxins, conjugation with antibodies 494
Toxins, encapsulated in liposomes 552
Toxins, gold-labeled 596
Toxins, handling and safety 498 506
Toxins, lectin binding properties of 497
Toxins, nicked 497
Toxins, proteins used in immunotoxins 496
Toxins, single-chain types 498
Toxins, thiolation using 2-iminothiolane 504 520 521
Toxins, two-chain types 497
Trail 517
Traincard 46 647
Transamination 313 361 390 645 661
Transamination of cytosine 44 357 645
Transfer RNA 41 55
Transferrin receptor OX-26 252
Transferrin, in cytotoxic conjugates 495
Transferrin, in cytotoxic conjugates, SPDP conjugation to toxins 505
Transglutaminase, assay of using 5-(biotinamido)-pentylamine 394
Transition temperature, liposomal, effect on immune response 550
Transmembrane glycoprotein H-2Kk 310
Traut 57 210
Traut’s reagent see “2-Iminothiolane”
Tresyl chloride 33
Trichloro-S-triazine 287 (see also “TsT”)
Trichloroacetic acid 406
Triethanolamine 59 144 204 510 562
Triethylamine 544 546 618
Trifluoroacetate 104
Trifunctional cross-linkers 149 287
Trifunctional cross-linkers, biocytin-containing 375
Triiodothyronine 427
Triple helix 36
Triplet excited state 300
Triplet transition 300
Triplet-state ketone intermediate 164 279
Triplett 172 176 438
Tris 294 473
Tris for blocking aldehydes 135
Tris for blocking carboxylates 136
Tris for quenching glutaraldehyde reactions 471 472
Tris in NHS ester reactions 191
Tris(2-carboxyethyl)phosphine see “TCEP”
Tris, blocking excess glutaraldehyde with 583
TRITC 317 320 670
TRITC, comparison to Lissamine rhodamine B 323
TRITC, coupling to diamine modified DNA 671
Triton X 532
tRNA 41 310 379 643
Troponin 308
Troponin C 212
Trubetskoy 366
Truneh 552
Trypanosome brucia 243
Trypsin 85 110 270 291 478
Trypsinogens 110
Tryptic hydrolysis, of aminoethylated cysteine residues 105
Tryptophan 6 570
Tryptophan, absorptivity 6
Tryptophan, oxidation by periodate 294
Tsai 212
Tseng 496
Tsien 324
TSK3000 SW 524
Tsomides 407
TsT, activation of mPEG using 608
TsT, activation of PEG using 607
TsT, reactivity of 607
TsT, toxicity of 608
TsT-mPEG 608
TsT-mPEG, modification of proteins with 609
Tsudo 196
Tsukamoto 88
Tsuro 193
TU 641
Tuberculin purified protein derivative 422
Tubulin, labeling with photobiotin 395
Tumor 367 490 492 494 620
Tumor antigens 494
Tumor necrosis factor 200
Tumor, localization using avidin-biotin interactions 574
Tumor, targeting of 365
Tumor, targeting with biotinylated antibodies 379
Tumor, targeting with liposomes 552
Turko 182
Tussen 474
Two-phase systems 607
Tyrosine 6 91 92 111 323 402 407 408 410 411
Tyrosine, absorptivity 12
Tyrosine, iodination of 163 365 402 403 405 406 490
Tyrosine, modification with  401
Tyrosine, pKa 12
Tyrosine, reaction with anhydrides 146
Tyrosine, reaction with diazonium 162 224 225 446
Tyrosine, reaction with EDC 172
Tyrosine, reaction with isothiocyanates 138
Tyrosine, reaction with maleic anhydride 129
Tyrosine, reaction with p-aminobenzoyl biocytin, diazotized 398
Tyrosine, reaction with tetranitromethane 110
Tyrosine, use as an iodination scavenger 259
Tyrosyl-lysine, conjugation to BSA 432
Tyrosyl-lysine, conjugation with KLH using EDC 433
Uchino 331
Uchiumi 208
Ueland 284
ulfhydryls, detection with TNBS 112
Ullman 305
Unilamellar vesicles (ULV) 427 529
Uracil 41
Uracil, biotin derivative of 656
Uracil, cycloaddition to 46
Uracil, formation from cytosine 44 645
Uracil, mercuration of 46
Uracil, reaction with CMC 45
Uracil, structure and reactivity 42
Uraki 128
Urdea 666
Urea 76 84 87
Urethane 143 155 156 158 184 202 355 611
Uronic acid 29 107
Uto 236
UV irradiation 229
Uyeda 95
Vaccines 22 419 421 429
Vaccines, liposome-containing 528 550
Valeric acid, (of biotin) 372 373 377 386 388 390 391 394 395 657 661
Valine 5
Valle 91
Vallee 446
Van Dalen 320
Van den Brink 600
Van der Horst 256
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