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Hermanson G.T. — Bioconjugate Techniques
Hermanson G.T. — Bioconjugate Techniques



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Название: Bioconjugate Techniques

Автор: Hermanson G.T.

Аннотация:

This is a well-organized and well-written book; it is readable and comprehensible to the novice, but its content and stylistic approach seem sufficiently sophisticated to appeal to active, knowledgeable workers in the field. It is an impressive compilation of useful theoretical and practical information that is not readily available elsewhere in a single volume.


Язык: en

Рубрика: Технология/

Статус предметного указателя: Готов указатель с номерами страниц

ed2k: ed2k stats

Год издания: 1996

Количество страниц: 785

Добавлена в каталог: 15.02.2007

Операции: Положить на полку | Скопировать ссылку для форума | Скопировать ID
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Предметный указатель
DNA, probe design      55
DNA, purine residues of      47
DNA, reaction with EDC      52
DNA, reaction with N-bromosuccinimide      648
DNA, structure      53
DNA, sugar constituents      50
DNA, sulfhydryl-modified, coupling of sulfhydryl-reactive fluorescent probes      671
DNA, synthesis      53
DNA, Watson-Crick model      54
DNase I      641
dNTP      643
Domen      102 186 324 325 550
Donovan      173
Dot blot      400
DOTA, general description and use      367
Dottavio-Martin      31
Double helix      54
Dovichi      304
Dowben      532
Dower      207
Downey      473
DPDPB, general description and use      209
DPPC      550 552
DPPE      543
DPPE activation with MBS      546
DPPE activation with SMPB      544 552
DPPG      550 552
Drafler      282
Draper      313 339 346 357 361 645 646
Dreborg      607
Dried milk, stabilization of colloidal gold suspensions using      595
Drugs, conjugation to toxins      517
Drugs, encapsulated in liposomes      552
Drugs, liposome encapsulation      528
DSC      29 142 156 612
DSC, activation of mPEG using      612
DSC, general description and use      202
DSC, reaction with hydroxyls      156
DSG, general description and use      201
DSP      191
DSP, general description and use      192
DSPE      543
DSS      666
DSS, DNA-alkaline phosphatase conjugation using      664
DSS, general description and use      194
DST      294
DST, general description and use      196
DTBP, general description and use      207
DTE      77 85
DTNB      151 see
DTPA      298 392
DTPA, general description and use      365
DTSSP, general description and use      193
DTT      75 76 79 85 87 104 107 130 132 134 150 192 212 231 240 252 308 310 504
DTT, disulfide cross-linkers      79
DTT, effect on aryl azides      254
DTT, general description and use      77
DTT, partial reduction of antibodies      523
DTT, pyridyl disulfides      276 371 508 519
DTT, reduction of AMCA-HPDP derivatives      335
DTT, reduction of APDP      277
DTT, reduction of aryl-thiol bond      150
DTT, reduction of biotin-HPDP-modified proteins      387
DTT, reduction of cystamine      501
DTT, reduction of cystamine-DNA      652
DTT, SADP      266
DTT, SAED      270
DTT, SASD      256
DTT, SMPT      66
DTT, SPDP      65 504
DTT, sulfo-NHS-SS-biotin modified proteins      382
DTT, sulfo-SBED cross-links      291
DTTA in time-resolved fluoroim-munoassays      368
DTTA, general description and use      368
du Vigneaud      373
Duband      324
Duijndam      313
Dunbar      80
Duncan      60 367 605
Dunn      182 606
Dunnick      559
Durand      338
dUTP, derivatives useful for enzymatic labeling of DNA      641
Dyes, with similar structures to fluorophores      300
Dysprosium      368
E. coli      see “Escherichia coli”
Ebrahim      375
EDAC      see “EDC”
EDC      73 75 101 103 125 136 338 560 649 650 651
EDC for conjugating [Met5]-enkephalin to OVA      433
EDC for conjugating, tyrosyl-lysine to KLH      433
EDC for conjugating, [Met5]-enkephalin to BSA      433
EDC for coupling hydrazides to car-boxylates      123 222
EDC for coupling PDTT      513
EDC in coupling ASBA to car-boxylates      284
EDC in hapten-carrier conjugation      429
EDC in modifying phosphates with bis-hydrazides      124
EDC in phosphoramidate formation      109
EDC in preparing cBSA      423
EDC, coupling to 5’-phosphates using      649
EDC, cystamine modification of DNA using      651
EDC, formation of NHS esters      140
EDC, general description and use      170
EDC, levels used with KLH      433
EDC, modification of avidin or streptavidin with ad-ipic acid dihydrazide      590
EDC, pH dependence of conjugation using      430
EDC, protein conjugation to Ц-posomes using      559
EDC, quenching with 2-mercap-toethanol      438
EDC, reaction with AMCA      332
EDC, reaction with amine-containing Cascade Blue derivatives      355
EDC, reaction with biotin      373
EDC, reaction with carboxylate-PEG      614
EDC, reaction with DFA      370
EDC, reaction with phosphate groups of nuclmtides      52
EDC, reaction with phosphatidic acid      539
EDC, reaction with sulfo-NHS      173
EDC, use in cationization      102
EDC, use in conjugating tyrosyl-lysine to BSA      432
EDC, use in forming hydrazide-protein derivatives      637
EDC/sulfo-NHS reaction      342 344
EDC/sulfo-NHS reaction in the activation of protein carboxylates      436
Edelhoch      208
Edelman      79
EDR-148      see “Jeffamine”
Edsall      208
EDTA      86 87 99 132 232 337 388 380 519 578 632
EDTA for elution from metal che-late affinity supports      486
EDTA for prevention of metal-catalyzed oxidation of sulfhydryls      57 343 363
Edwards      15 239 504 397
Effectomer      497
EGF      73
Egg      542
Egg lecithin      550 see
Egg whites      373
Egg yolk      533
EGS, general description and use      199
EGS, hydroxylamine cleav-ability      295
Eiklid      497
Eisen      143 150
Eldjarn      69 86
Electromagnetic radiation      400
Electron      400
Electron capture      400
Electron donating      402
Electron donating groups, effect on fluorescence      299
Electron microscopy      389 593
Electron microscopy, use of colloidal gold labeling      593
Electron withdrawing groups, effect on fluorescence      299
Electrons      298 300
Electrophilic      162 214
Electrophilic, halogen species      403
Electrophilic, reactions      8
Electrophilic, reactions of tyrosine      12
Electrophilic, species      491
Electrophilic, substitution      403
Electrophoresis      80 103 208
ELISA      243 325 326 328 360 365 374 376 378 630 634 662
ELISA, using avidin-biotin interactions      575
ELISA, using liposomes in      553
Ellis      600
Ellman      88 132 151
Ellman's assay      99 510 521
Ellman's reagent      57 87 133 134
Ellman's reagent for determining the level of maleimide activation      445
Ellman's reagent in activating sulfhydryls      151
Ellman's reagent in measuring maleimide activation levels      442
Ellman's reagent in preparing immunotoxins      515
Ellman's reagent, general description and use      88 132
Emulsification      566
Emulsification of lipids      532
Emulsion, water-in-oil      533
Encapsulation      528
Encapsulation of molecules into Upo-somes      550
End ester      182
Endocytic vesicles      497
Endocytosis      553
Endosomes      419
Energy transfer      301 364 670
Englund      131
Enoch      532
Enol      32
enzyme      456
Enzyme in the design of immu-notoxic conjugates      495
Enzyme, activation with bis-hydrazides      222
Enzyme, activation with glutaraldehyde      472 584
Enzyme, activation with periodate      472
Enzyme, activation with SMCC      461 576 578
Enzyme, activation with SPDP      637
Enzyme, activation with sulfo-SMCC      578
Enzyme, avidin- or streptavidin-labeled      298 572
Enzyme, biotinylated      222 392 493 572 637
Enzyme, conjugation to avidin using glutaraldehyde      583
Enzyme, conjugation to DNA      662
Enzyme, conjugation with antibodies      460
Enzyme, conjugation with removal of unconju-gated enzyme      483
Enzyme, for the oxidation of sugar residues      157
Enzyme, gold-labeled      596
Enzyme, hydrazide derivatives of      222 377 637
Enzyme, hydrazide- activated      477
Enzyme, immobilized      410
Enzyme, maleimide activation of      461
Enzyme, maleimide-activated      467 469 383
Enzyme, maleimide-activated, conjugation with reduced antibodies      463
Enzyme, modification with bis-hydrazide reagents      121
Enzyme, modification, conjugation of, and      630
Enzyme, oxidation with periodate      474
Enzyme, polymer modification of      605
Enzyme, reactive derivatives for conjugation      635
Enzyme, substrates for detection      630
Enzyme, types and properties      630
Enzyme, unconjugated      487
Enzyme-linked immunosor-bent assay      see “ELISA”
Enzymobeads, in the iodination of proteins      410
Epichlorohydrin, activation of PEG with      617
Epidermal growth factor modification with cystamine      515
Epidermal growth factor modification with cystamine, SPDP conjugation to toxins      505
Epitope      420
Epitope mapping      600
Epoxide      33
Epoxide, chemistry of      142
Epoxide, general information      220
Epoxide, modification of dextran      626
Epoxide, reaction pyrimidines      44
Epoxide, reaction with ammonium ion      142
Epoxide, reaction, conditions      155 221
Epoxide, transformation to an aldehyde      142
Epps      149
Ericsson      320 325 330
Ernsting      331
Erythrocyte      194 196 205 208 212 266 310
Erythrocyte, ghosts      256
Erythropoietin      196
Erythrose      27
Eschericbia colt      208 633
Eschrich      331
Esters      94
Esters in deavable reagents      293
Esters, hydroxylamine-deavable      295
Ethanol      532 633
Ethanolamine      135 136 144 186 294 373 376 378
Ethanolamine for blocking aldehydes      135 582
Ethanolamine for blocking carboxylates      136
Ethanolamine, blocking excess glutaraldehyde with      471 472 583
Ether, formation with epoxides      220
Ethyl acetate      618
Ethylene glycol group, cleavabilityof      199
Ethylene glycol, in crossbridge of EGS      296
Ethylene glycolbis (succinimidylsuccinate)      see “EGS”
Ethylene glycolbis(sulfosuccinimidylsuccinate)      see “Sulfo-EGS”
Ethylene oxide      606
Ethylene oxide as repeating unit in PEG      605
Ethylenediamine      101 110 623 624 625 645 647
Ethylenediamine in the preparation of cBSA      423
Ethylenimine      149
Ethylenimine, comparison to Aminoethyl-8      104
Ethylenimine, general description and use      105
Ethylenimine, produced from 2-bromoethylamine      107
Ethylenimine, reaction with pyrimidines      44
Etzler      428
Europium      368
Ewig      227
Excitation      298 301
Excited-state      301
Extinction coefficient      298
Extrusion, of lipids      532
F-actin      208
F/P ratio      304 307 320 324 326 328 331 335 337 339 340 341
FAB      501
Fab' fragments      236 243 459 501
Fab' fragments, conjugation to toxins, using Ellman’s reagent      516
Fab' fragments, conjugation to toxins, using S-sulfonates      516
Fab, fragments      458 486 496
Fab, preparation of      478 480
Fabrics      607
Factor V      258
Factor Va      328
Factor XIII      394
Factor Xllla, assay of using 5-(biotinamido)pentylamine      394
FAD      19 634
Fahien      208
Fairbanks      139 140 191 192 609
Falke      310
Farmer      666
Farr      478
Farries      196
Fasold      209 211
Fatty acid alcohols      539
Fatty acids      427 528
Fatty acids in egg PE      543
Fatty acids in phosphodiglycerides      534
Fatty acids in phospholipids      533
Fatty acids in sphingolipids      536
Fatty acids, HPLC analysis of      153
Fatty acids, immunogenicity of      550
Fatty acids, unsaturated      535
Faulk      593 597
FC      249
Fc, fragments      458 480
Fc, receptors      196 207
Fe+3      128
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
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