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                    | Hermanson G.T. — Bioconjugate Techniques |  
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                    | Предметный указатель |  
                    | | Antigen-MHC complex      420 Antigenic determinants      499 552
 Antigenic specificity      456
 Antigenicity, of liposomal vesicles      549
 Antigens      428
 Antigens, attachment to liposomes      528
 Antigens, detection of using fluorescently labeled antibodies      489
 Antimisiaris      550
 Antineoplastic      620
 Antioxidants      410
 Antiparallel      54
 Aorta      196
 APDP, general description and use      277
 APDP, iodination of      416
 Apkarian      620
 Aplysia gonad lectin      601
 Apple      102 424 550
 Arabinose      27
 Arginine      6
 Arginine vasopressin, conjugation with cBSA      425
 Arginine, pKa      7
 Arginine, reaction of guanidinyl group with glyoxals      284
 Arginine, reaction with APG      285
 Arginine, reaction with glyoxal compounds      160
 Argon laser      325 330
 Argon-krypton mixed laser      325 330
 Armstrong      375
 Arsenate      632
 Aryl amine      143
 Aryl azides      254 276 375
 Aryl azides, effect of hydroxyl groups on the ring      254
 Aryl azides, effect of nitro groups on the ring      254
 Aryl azides, general properties      214
 Aryl azides, halogenated      163
 Aryl azides, in biotinylation reagents      394
 Aryl azides, iodinatable      415
 Aryl azides, of ABH      282
 Aryl azides, of ABNP      288
 Aryl azides, of ANB-NOS      263
 Aryl azides, of APDP      277
 Aryl azides, of APG      285
 Aryl azides, of ASBA      284
 Aryl azides, of ASIB      276
 Aryl azides, of HSAB      260
 Aryl azides, of NHS-ASA      255
 Aryl azides, of photobiotin      395
 Aryl azides, of SADP      265
 Aryl azides, of SAED (AMCA derivative)      268
 Aryl azides, of SAND      263
 Aryl azides, of SANPAH      261
 Aryl azides, of SASD      257
 Aryl azides, of sulfo-SAMCA (AMCA derivative)      272
 Aryl azides, of sulfo-SAPB      267
 Aryl azides, of sulfo-SBED      289
 Aryl azides, perfluorinated      254 255
 Aryl azides, reaction of      163
 Aryl azides, reaction with sulfhydryls      254
 Aryl azides, ring expansion of      254
 Aryl halides      212 213
 Aryl halides, chemistry of      143
 Aryl halides, reactions of      149
 Arylamines      31 111
 Arylamines, formation by reaction with diflurobenzene derivatives      212
 Arylating agents, reactions of      149
 ASBA, general description and use      284
 ASBA, iodination of      416
 Ascorbic acid      19 29 142 630
 Ascorbic acid for reductive amination      186
 Ashwell      36 108 115
 Asialofetuin, immobilized      501 522
 ASIB, general description and use      276
 Asparagine      6 36
 Aspartic acid      6
 Aspartic acid pKa      7
 Aspergillus niger      634
 Aspergillus Restrictus      497
 Astatine      403
 Atassi      96 129 614
 Atha      313
 Atkinson      196
 ATP      19 51
 ATPase      206 208 310 313 324
 Atrial natriuretic factor      196
 Autofluorescence      332
 Avian sarcoma virus      208
 Avidin      254 287 298 371 378 382 384 386 388 390 492 661
 Avidin as bridging molecule      492 638
 Avidin as egg-white protein      373
 Avidin, activation with bis-hydrazides      222
 Avidin, activation with sulfo-SMCC      576
 Avidin, affinity constant with biotin      570
 Avidin, binding to ABNP cross-links      288
 Avidin, binding to biotinylated PE in liposomes      554
 Avidin, binding to biotinylated phospholipids      554
 Avidin, binding to sulfo-SBED cross-links      289
 Avidin, biotin binding site      372
 Avidin, comparison to streptavidin      571
 Avidin, complex with biotinylated enzyme      493
 Avidin, conjugation of      570
 Avidin, conjugation of, reductive amination      580
 Avidin, conjugation of, to ferritin using glutaraldehyde      583
 Avidin, conjugation of, to HRP using reductive animation      581
 Avidin, conjugation of, to liposomes      553
 Avidin, conjugation of, using glutaraldehyde      583
 Avidin, conjugation of, with enzymes using SMCC      576
 Avidin, conjugation of, with ferritin using reductive amination      580
 Avidin, conjugation of, with HRP using reductive amination      582
 Avidin, conjugation of, with phycobiliproteins      363
 Avidin, conjugation of, with phycoerythrin      588
 Avidin, denaturation of      571
 Avidin, double labeling systems using      585
 Avidin, effect of periodate      294
 Avidin, enzyme conjugate of      375 395 572 575
 Avidin, fluorescent labeling of      574 584
 Avidin, general description and use      570
 Avidin, gold labeling of      602
 Avidin, gold-labeled      574 596
 Avidin, HABA binding characteristics      591
 Avidin, hydrazide-activated      222 574 637
 Avidin, immobilized      291 380 382 387 394
 Avidin, interaction with biotin      373 488 570
 Avidin, interaction with biotinylated antibodies      488 492
 Avidin, interaction with biotinylated liposomes      558
 Avidin, interaction with iminobiotin      372 380
 Avidin, labeling with gold      603
 Avidin, maleimide-activated      578
 Avidin, modification with adipic dihydrazide      121 589
 Avidin, modification with AMCA-NHS      587
 Avidin, modification with FITC      585
 Avidin, modification with Lissamine rhodamine sulfonyl chloride      586
 Avidin, nonspecific binding properties      571
 Avidin, optimal fluorescein labeling level      586
 Avidin, optimal molar ratio to enzyme in conjugates      582
 Avidin, phycoerythrin conjugation of      585
 Avidin, preparation of conjugates      575
 Avidin, purification using immobilized iminobiotin      381
 Avidin, radiolabeled      574
 Avidin, structure and properties      570
 Avidin, thiolation of, using 2-iminothiolane      588
 Avidin, thiolation of, using SATA      62 579
 Avidin, toxin conjugate      495 574
 Avidin, use as a bridge in immunoassays      572
 Avidin, use in assay systems      572
 Avidin-biotin assay      394
 Avidin-biotin complex (ABC)      372 493
 Avidin-biotin system      492 570
 Avidin-enzyme conjugate      492
 Avigad      31 108 116
 Avrameas      22 187 220 453 470
 Axon      322
 
 | Azide      632 Aziridines in polyethylenimine      106
 Aziridines, reactions of      148
 Azlactone, formation from DCC reaction      180
 AZT      93
 B cell      208 620
 B chain      499 502
 B chain, blocking binding site of      501 517
 B chain, blocking of by lactose cross-linker      501
 B chain, cleavage from A chain      499
 B chain, nonspecific binding      521 524
 B chain, of diphtheria toxin      497
 B chain, of plant toxins      497
 B chain, of ricin, function of      497
 B chain, removal of binding potential by affinity chromatogra-phy      501
 B-PE      see “PE biotinylated”
 Bacha      75
 Bachhawat      607 620
 Bacillus stearothermopbilus      207
 Backgrounds      490
 bacteria      420 525
 Baenziger      262 266
 Baird      208
 Bakkus      586
 Balbinder      177
 Ball      304
 Ballmer-Hofer      260 262
 Balls      128
 Bangham      532
 Bangs      243
 Baranowska-Kortylcwicz      331
 Barany      178
 Barbieri      498
 Barbour      473
 Barley yellow dwarf virus      396
 Bartel      422
 Bartling      154 183 615
 Basala      513
 Base labile      198
 Base pairing      40 384 639 647 663
 BASED, general description and use      214
 BASED, iodination of      416
 Baskin      193 200 208
 Batzri      532
 Baues      31
 Bayer      108 115 116 117 121 123 222 373 385 393 571 572 580 583 586 589 637
 Bayne      208
 Bayse      410
 BCA protein assay      464 367
 BCA protein assay reagent, assay of polyaldehyde dextran using      622
 BCAs      see “Bifunctional”
 Beauchamp      155 184 616 617
 Belew      486
 Bendayan      599
 Benesch      69 215
 Benhamou      601
 Benzaldehyde      32
 benzene      608
 Benzidine      224 349
 Benzidine, diazotized      225
 Benzidine, diazotized, use in preparing hapten-carrier conjugates      448
 Benzoic acid      260 263 522
 Benzophenone      254 255 276 279
 Benzophenone of benzophcnone-4-malcimide      281
 Benzophenone of benzophenone-4-iodo-acetamide      279
 Benzophenone, photoactivation mechanism      279
 Benzophenone, reactions of      164
 Benzophenone-4-iodoacetamide      279 280
 Benzophenone-4-iodoacetamide, general description and use      279
 Benzophenone-4-maleimide, general description and use      280
 Benzyl halides      147
 Benzyl halides, reactions of      226
 Berg      212
 Berger      606 607
 Bergmann      166
 Bergstrom      607 617
 Bernstein      620 621
 Beth      173
 Bethell      155 184
 Beutncr      304
 Bewley      78
 Bidnchoninic acid      622 see
 Biermann      36
 Bifunctional chelating agents (BCAs)      365
 Bifunctional chelating agents (BCAs), DOTA, NOTA, and TETA      367
 Bifunctional chelating agents (BCAs), DTPA      365
 Bifunctional chelating agents (BCAs), DTTA      368
 Bifunctional chelating agents (BCAs), general description and use      365
 Bigelow      310
 Bilayer membranes      528 533 537 540 558
 Bilayer membranes, effect of cholesterol      537
 Bilayer membranes, stabilizing in presence of organic solvent      547
 Bilin prosthetic groups      362 588
 Biliproteins      362
 Biliproteins, conjugation with avidin or streptavidin      588
 Bimbaumer      59
 Binder      595
 Binkley      35
 Biocompatibility      605
 Biocompatibility, effect of PEG modification      606
 Biocytin      392
 Biocytin hydrazide, general description and use      392
 Biocytin hydrazide, glycoprotein labeling protocol      393
 Biocytin in constructing trifunc-tional cross-linkers      287
 Biocytin in p-aminobenzoyl biocytin      398
 Biocytin of sulfo-SBED      289
 Biocytin, as fundamental unit of sulfo-SBED      375
 Biocytin, general description and use      375
 Biocytin, hydrazide derivative      637
 Biocytin, reaction with biotinidasc      375
 Biocytin, use in trifunctional cross-linking reagents      375
 Biotin      75 254 371 356 592 637 661
 Biotin of ABNP      288
 Biotin, analog of      372
 Biotin, general description and use      373 570
 Biotin, importance of valeric acid side chain      373
 Biotin, interaction with avidin      570
 Biotin, interaction with streptavidin      571
 Biotin, lysine derivative of      375
 Biotin, modification of biomolecules with      591
 Biotin, modification of dextran with      620
 Biotin, modification of phycobiliproteins with      363
 Biotin, NHS ester derivative of      375
 Biotin, protein cofactor      19
 Biotin, reactive derivatives of      372
 Biotin, use in assay systems      572
 Biotin-11-dUTP      656
 Biotin-binding sites      492
 Biotin-BMCC      660
 Biotin-BMCC, general description and use      384
 Biotin-BMCC, modification of
  -galactosidase with      638 Biotin-BMCC, modification of sulfhydryl-DNA probes      660
 Biotin-BMCC, protein modification protocol      386
 Biotin-dATP      656
 Biotin-dCTP      656
 Biotin-dUTP      656
 Biotin-HPDP, general description and use      386
 Biotin-HPDP, protein modification protocol      388
 Biotin-hydrazide      32 662
 Biotin-hydrazide, comparison to NHS-LC-biotin      379
 Biotin-hydrazide, coupling to cytosine groups      661
 Biotin-hydrazide, cytosine modification with      646
 Biotin-hydrazide, general description and use      390
 Biotin-hydrazide, glycoprotein labeling protocol      392
 Biotin-LC-hydrazide, general description and use      391
 Biotin-LC-hydrazide, glycoprotein labeling protocol      392
 Biotinidase      375
 Biotinylated antibody      488
 Biotinylation of antibodies      476 388 392
 Biotinylation of DNA      656
 
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