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| Авторизация |
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| Поиск по указателям |
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| Hermanson G.T. — Bioconjugate Techniques |
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| Предметный указатель |
Hydrazine, reaction with aldehydes 159
Hydrazine, reaction with nucleotides 43
Hydrazone 32 115 121 135 159 222 223 249 313 314 315 328 346 362 371 390 392 589 661
Hydrazone, reduction with sodium cyanoborohydride 121 392 478
Hydrogen 88
Hydrogen bonding 537
Hydrogen bonding, of nucleotide base pairs 53
Hydrogen peroxide 401 630 634
Hydrogen peroxide oxidoreductase see “HRP”
Hydrogen, active 161
Hydrogenolysis 293
Hydroxyl groups in the formation of carbonates 142
Hydroxyl groups, activation with CDI 155 183
Hydroxyl groups, activation with DSC 202
Hydroxyl groups, cross-linking with PMPI 138
Hydroxyl groups, formation by reduction of aldehydes 186
Hydroxyl groups, nucleophilicity 33
Hydroxyl groups, oxidation of diols with periodate 114
Hydroxyl groups, reaction of 154
Hydroxyl groups, reaction with alkyl halides 158 227
Hydroxyl groups, reaction with anhydrides 91 146
Hydroxyl groups, reaction with chloroacetic acid 100
Hydroxyl groups, reaction with epoxides 142 155 220
Hydroxyl groups, reaction with glutaric anhydride 94
Hydroxyl groups, reaction with isocyanate 138 158 355
Hydroxyl groups, reaction with NHS esters 139 191
Hydroxyl groups, reaction with NHS-chloroformate 156
Hydroxyl groups, reaction with sodium periodate 156
Hydroxylamine 33 469 558 655
Hydroxylamine for cleaving ester bonds 199
Hydroxylamine for cleaving O-acetyltyrosine 128
Hydroxylamine in the cleavage of estercontaining cross-linkers 92 295
Hydroxylamine reaction with periodate 157
Hydroxylamine reaction with pyrimidines 43
Hydroxylamine reaction with SAMSA 70
Hydroxylamine, assay of polyaldehydedextran using 622
Hydroxylamine, deprotection of acetylated sulfhydryls with 579
Hydroxylamine, deprotection of acetylated sulfhydryls with SATA using 61 467 578
Hydroxylamine, deprotection of acetylated sulfhydryls with SATP 63
Hydroxylamine, use in deblocking citraconylated amines 97
Hydroxylysine 36
Hynes 36
Hypervariable region 82 458 496
IA antigens 208
Ibrahim 109 144 170
Ichishima 621
ICl 162 403 408
IgA 457
IgA, structure of 457
IgD 457
IgE 194 196 457
IgE, biotinylation of 377
IgG 422 457
IgG, activation with periodate 475
IgG, binding to nickel chelate, affinity columns 486
IgG, conjugation to liposomes 542
IgG, conjugation with cBSA 425
IgG, detection using biotinylated immunoglobulin binding proteins 574
IgG, digestion with papain 480
IgG, digestion with pepsin 479
IgG, enzymatic digestion of 458 478
IgG, gold labeling 595
IgG, isolation of monomeric form 511
IgG, labeling with biotin-hydrazide 390
IgG, labeling with NHS-fluorescein 306
IgG, labeling with NHS-rhodamine 322
IgG, reduction of disulfides 76 463
IgG, reduction of disulfides with 2-mercapto-ethylamine 82 461
IgG, reduction of disulfides, using TCEP 84
IgG, reductive amination with enzymes 475
IgG1 480
IgG2 481
IgG3 480
IgG4 480
IgM 457
IgM, structure of 457
Ikai 207
Imagawa 236
Imaging 367 379 490 552
Imidate see “Imidoester”
Imidazole 8 98 109 124 153 155 183 184 185 411 468 615 649 650 651 652
Imidazole in NHS ester reactions 191
Imidazole, iodination of 163
Imidazole, modification with  401
Imidazole, reaction with diazonium 162 225
Imidazole, reaction with NHS esters 139
Imidazole, ring of purines 47
Imidazolyl carbamate 143 155 183 611 615
Imidazolyl carbamate, hydrolysis of 185
Imide, as the product of a hydrazide reaction with a carboxylate 123
Imidoamide 144 (see also “Amidine”)
Imidoesters 57 190 562
Imidoesters of DMA 204
Imidoesters of DMP 205
Imidoesters of DMS 206
Imidoesters of DTBP 207
Imidoesters, chemistry of 143
Imidoesters, homobifunctional 203
Imine 109 159
Iminobiotin 372
Iminobiotin, immobilized 381
Iminobiotin, NHS ester of 380
Iminodiacetic acid, immobilized 486
Immobilization 131 186 206 216
Immobilization, using CDI 184
Immobilized disulfide reductants 85 86
Immobilized protein A 480
Immonium cation intermediate, in formaldehyde reactions 216
Immune complexes 207
Immune modulators 550
Immune response 419
Immune response in a hapten-carrier system 420
Immune response toward cationized molecules 101
Immune response toward cBSA immunogens 424
Immune response toward precipitated immunogens 423
Immune response toward proteins 422
Immune response, effect of liposomal transition temperature 550
Immune response, effect of positively-charged liposomes 550
Immune response, modulation through PEG modification 606
Immune response, reduction of 605
Immunity, definition of 419
Immunity, protective 421
Immunization 420 426
Immunization with liposome carriers 428
Immunoaffinity chromatography 206 484
Immunoaffinity chromatography, protocol 485
Immunoassay 231 379 483 493 570 578
Immunoassay, avidin-biotin complex system (ABC) 572
Immunoassay, bridged avidin-biotin system (BRAB) 572
Immunoassay, labeled avidin-biotin system (LAB) 572
Immunoassay, liposome-mediated (LISA) 528 553
Immunoassay, radioactive (RIA) 488 490
Immunochemical blocking agent 595
Immunocytochemical staining 600 631
Immunocytochemistry 429
Immunogen 22 224 236 239 243
Immunogen conjugation 172
Immunogen conjugation, using EDC 171
Immunogen, definition of 419
Immunogen, gold as carrier 596
Immunogen, hapten-polymer conjugates 605
Immunogen, liposome-containing 528 537 550
Immunogen, preparation of 429
Immunogenicity 420 421
Immunogenicity of cBSA 423
Immunogenicity of cross-linker cross-bridges 229 441
Immunogenicity of KLH 422
Immunogenicity of liposomes 550
Immunogenicity of PEG-modified molecules 607
Immunogenicity of polymerized haptens 429
| Immunogenicity, modulation by modification with dextran 620
Immunoglobulin 85
Immunoglobulin binding proteins 456
Immunoglobulin binding proteins, biotinylated 574
Immunoglobulin binding proteins, gold-labeled 596 599
Immunoglobulin binding proteins, immobilized 484
Immunoglobulin on B lymphocytes 419
Immunoglobulin, activation with periodate 475
Immunoglobulin, binding to nickel chelate affinity column 486
Immunoglobulin, biotinylated 492
Immunoglobulin, biotinylation of 488
Immunoglobulin, class 457
Immunoglobulin, conjugation to liposomes by reductive amination 562
Immunoglobulin, conjugation, using carbonyl—and sulfhydryl-reactive cross-linkers 249
Immunoglobulin, directed against immunotoxin conjugates in vivo 496
Immunoglobulin, FITC-labeled 304
Immunoglobulin, glycosylation sites 458
Immunoglobulin, gold labeling of 595 601
Immunoglobulin, labeled 487
Immunoglobulin, labeling with AMCA-hydrazide 340
Immunoglobulin, labeling with biotin-hydrazide 390
Immunoglobulin, modification with DTPA 366
Immunoglobulin, modification with sulfo-NHS-biotin 377
Immunoglobulin, modification, conjugation of, and 456
Immunoglobulin, optimal fluorophore incorporation 490
Immunoglobulin, radiolabeling of 488 491
Immunoglobulin, reduction of 463
Immunoglobulin, reduction of, using DTT 78
Immunoglobulin, reduction of, with 2-mercaptoethylamine 82
Immunoglobulin, structure of 457
Immunoglobulin, subclasses 463
Immunoglobulin, thiolation of 461
Immunoglobulin, thiolation of, using 2-iminothiolane 465
Immunoglobulin, thiolation of, using PDPH 371
Immunogold staining 593
Immunohistochemical staining 83 320 473 478 584 587 633 635
Immunohistochemical staining, using fluorescently labeled antibodies 489
Immunotoxins 150 231 233 239 243 252 496 553
Immunotoxins of protein toxins 497
Immunotoxins, antibody-to-toxin ratio 503
Immunotoxins, conjugation using intact A-B toxins 516
Immunotoxins, conjugation using PDTP 513
Immunotoxins, conjugation using SPDP 504
Immunotoxins, cytotoxicity of 501
Immunotoxins, cytotoxicity relative to cleavable cross-linkers 516
Immunotoxins, design of 499
Immunotoxins, gel filtration isolation of 513
Immunotoxins, general description and use 494
Immunotoxins, half-life in vivo 511
Immunotoxins, ideal characteristics 501
Immunotoxins, immune response toward 496
Immunotoxins, injection of 495
Immunotoxins, large-scale preparation of 511
Immunotoxins, modification with synthetic polymers 496
Immunotoxins, need for cleavable linker in 499
Immunotoxins, noncleavable cross-linkers in preparation of 500
Immunotoxins, nonspecific binding of 501
Immunotoxins, preparation of 499
Immunotoxins, preparation of strategies 500
Immunotoxins, preparation of, using amine—and sulfhydryl—reactive heterobifunctionals 516
Immunotoxins, preparation of, using aromatic maleimide cross-linkers 522 524
Immunotoxins, preparation of, using cystamine 514
Immunotoxins, preparation of, using Ellman’s reagent 515
Immunotoxins, preparation of, using MBS 522
Immunotoxins, preparation of, using reductive amination 525
Immunotoxins, preparation of, using S-sulfonates 516
Immunotoxins, preparation of, using SIAB 517
Immunotoxins, preparation of, using SMCC 519
Immunotoxins, preparation of, using SMPT 511
Immunotoxins, preparation of, using SPDP 503
Immunotoxins, preparation of, via disulfide exchange 503
Immunotoxins, protein toxins used in 497
Immunotoxins, removal of unconjugated toxin 524
Inada 606
Indium-111 365
Indium-114m 365
Indium-115 365
Indium-III 492
Inesi 310
Influenza virus 208
Ingalls 377
Initiation factor IF2 208
Inman 112 446 624
Innis 640
Insulin 75 86 243 287
Insulin receptor 260 375
Insulin, biotinylation of 376
Insulin-dependent protein kinases 196
Interferon 196
Interleukin 2 196
Interleukin-3 receptor 258
Intermediate filament 324
Intraperitoneally 425
Iodide 403
Iodination, changes in proteins as a result of 402
Iodination, controlling the reaction 403
Iodination, effect on protein activity 402
Iodination, groups susceptible toward 402
Iodination, indirect labeling methods 412
Iodination, methods 401
Iodination, methods of APDP 277
Iodination, methods of ASBA 284
Iodination, methods of ASIB 276
Iodination, methods of cross-linkers 411
Iodination, methods of histidine 8 402
Iodination, methods of NHS-ASA 256
Iodination, methods of SASD 257
Iodination, methods of tyrosine 402
Iodination, oxidizing agents used with 491
Iodination, process of 401
Iodination, reactions of 162
Iodination, reagents 400
Iodination, scavengers 259
Iodination, termination of 403
Iodination, using chloramine-T 403
Iodination, using IODO-BEADS 405
Iodination, using IODO-GEN 407
Iodination, using lactoperoxidase 410
Iodination, using modification agents 411
Iodine 162 240 351 360 412 568
Iodine, radioactive 491
Iodine, reaction with tyrosine 12
Iodine, reduction of 403
Iodine-125 365
Iodine-131 365
IODO-BEADS 214 491
IODO-BEADS, general description and use 405
IODO-GEN 214 258 401 491
IODO-GEN for iodination of SASD 259
IODO-GEN, general description and use 407
IODO-GEN, iodination protocol 409
Iodoacetamide 99 131 569
Iodoacetamide of Lucifer Yellow 359
Iodoacetamide, blocking unreacted sulfhydryl groups with 519
Iodoacetate 106
Iodoacetate for blocking sulfhydryls 131
Iodoacetate, general description and use 98
Iodoacetate, modification of amino-dextran with 624
Iodoacetate, modification of PE 542
Iodoacetate, relative reactivity of 99 147
Iodoacetic anhydride, modification of PE 568
Iodoacetyl 276 307 311 335 350 504 542
Iodoacetyl in biotinylation reagents 384
Iodoacetyl of ASIB 276
Iodoacetyl of benzophenone-4-iodoacetamide 279
Iodoacetyl of BODIPY 530/550 IA 351
Iodoacetyl of BODIPY FL IA 350
Iodoacetyl of DCIA 337
Iodoacetyl of iodoacetyl-LC-biotin 388
Iodoacetyl of Lucifer Yellow io-doacetamide 359
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