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                    | Hermanson G.T. — Bioconjugate Techniques |  
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                    | Предметный указатель |  
                    | | Hydrazine, reaction with aldehydes      159 Hydrazine, reaction with nucleotides      43
 Hydrazone      32 115 121 135 159 222 223 249 313 314 315 328 346 362 371 390 392 589 661
 Hydrazone, reduction with sodium cyanoborohydride      121 392 478
 Hydrogen      88
 Hydrogen bonding      537
 Hydrogen bonding, of nucleotide base pairs      53
 Hydrogen peroxide      401 630 634
 Hydrogen peroxide oxidoreductase      see “HRP”
 Hydrogen, active      161
 Hydrogenolysis      293
 Hydroxyl groups in the formation of carbonates      142
 Hydroxyl groups, activation with CDI      155 183
 Hydroxyl groups, activation with DSC      202
 Hydroxyl groups, cross-linking with PMPI      138
 Hydroxyl groups, formation by reduction of aldehydes      186
 Hydroxyl groups, nucleophilicity      33
 Hydroxyl groups, oxidation of diols with periodate      114
 Hydroxyl groups, reaction of      154
 Hydroxyl groups, reaction with alkyl halides      158 227
 Hydroxyl groups, reaction with anhydrides      91 146
 Hydroxyl groups, reaction with chloroacetic acid      100
 Hydroxyl groups, reaction with epoxides      142 155 220
 Hydroxyl groups, reaction with glutaric anhydride      94
 Hydroxyl groups, reaction with isocyanate      138 158 355
 Hydroxyl groups, reaction with NHS esters      139 191
 Hydroxyl groups, reaction with NHS-chloroformate      156
 Hydroxyl groups, reaction with sodium periodate      156
 Hydroxylamine      33 469 558 655
 Hydroxylamine for cleaving ester bonds      199
 Hydroxylamine for cleaving O-acetyltyrosine      128
 Hydroxylamine in the cleavage of estercontaining cross-linkers      92 295
 Hydroxylamine reaction with periodate      157
 Hydroxylamine reaction with pyrimidines      43
 Hydroxylamine reaction with SAMSA      70
 Hydroxylamine, assay of polyaldehydedextran using      622
 Hydroxylamine, deprotection of acetylated sulfhydryls with      579
 Hydroxylamine, deprotection of acetylated sulfhydryls with SATA using      61 467 578
 Hydroxylamine, deprotection of acetylated sulfhydryls with SATP      63
 Hydroxylamine, use in deblocking citraconylated amines      97
 Hydroxylysine      36
 Hynes      36
 Hypervariable region      82 458 496
 IA antigens      208
 Ibrahim      109 144 170
 Ichishima      621
 ICl      162 403 408
 IgA      457
 IgA, structure of      457
 IgD      457
 IgE      194 196 457
 IgE, biotinylation of      377
 IgG      422 457
 IgG, activation with periodate      475
 IgG, binding to nickel chelate, affinity columns      486
 IgG, conjugation to liposomes      542
 IgG, conjugation with cBSA      425
 IgG, detection using biotinylated immunoglobulin binding proteins      574
 IgG, digestion with papain      480
 IgG, digestion with pepsin      479
 IgG, enzymatic digestion of      458 478
 IgG, gold labeling      595
 IgG, isolation of monomeric form      511
 IgG, labeling with biotin-hydrazide      390
 IgG, labeling with NHS-fluorescein      306
 IgG, labeling with NHS-rhodamine      322
 IgG, reduction of disulfides      76 463
 IgG, reduction of disulfides with 2-mercapto-ethylamine      82 461
 IgG, reduction of disulfides, using TCEP      84
 IgG, reductive amination with enzymes      475
 IgG1      480
 IgG2      481
 IgG3      480
 IgG4      480
 IgM      457
 IgM, structure of      457
 Ikai      207
 Imagawa      236
 Imaging      367 379 490 552
 Imidate      see “Imidoester”
 Imidazole      8 98 109 124 153 155 183 184 185 411 468 615 649 650 651 652
 Imidazole in NHS ester reactions      191
 Imidazole, iodination of      163
 Imidazole, modification with
  401 Imidazole, reaction with diazonium      162 225
 Imidazole, reaction with NHS esters      139
 Imidazole, ring of purines      47
 Imidazolyl carbamate      143 155 183 611 615
 Imidazolyl carbamate, hydrolysis of      185
 Imide, as the product of a hydrazide reaction with a carboxylate      123
 Imidoamide      144 (see also “Amidine”)
 Imidoesters      57 190 562
 Imidoesters of DMA      204
 Imidoesters of DMP      205
 Imidoesters of DMS      206
 Imidoesters of DTBP      207
 Imidoesters, chemistry of      143
 Imidoesters, homobifunctional      203
 Imine      109 159
 Iminobiotin      372
 Iminobiotin, immobilized      381
 Iminobiotin, NHS ester of      380
 Iminodiacetic acid, immobilized      486
 Immobilization      131 186 206 216
 Immobilization, using CDI      184
 Immobilized disulfide reductants      85 86
 Immobilized protein A      480
 Immonium cation intermediate, in formaldehyde reactions      216
 Immune complexes      207
 Immune modulators      550
 Immune response      419
 Immune response in a hapten-carrier system      420
 Immune response toward cationized molecules      101
 Immune response toward cBSA immunogens      424
 Immune response toward precipitated immunogens      423
 Immune response toward proteins      422
 Immune response, effect of liposomal transition temperature      550
 Immune response, effect of positively-charged liposomes      550
 Immune response, modulation through PEG modification      606
 Immune response, reduction of      605
 Immunity, definition of      419
 Immunity, protective      421
 Immunization      420 426
 Immunization with liposome carriers      428
 Immunoaffinity chromatography      206 484
 Immunoaffinity chromatography, protocol      485
 Immunoassay      231 379 483 493 570 578
 Immunoassay, avidin-biotin complex system (ABC)      572
 Immunoassay, bridged avidin-biotin system (BRAB)      572
 Immunoassay, labeled avidin-biotin system (LAB)      572
 Immunoassay, liposome-mediated (LISA)      528 553
 Immunoassay, radioactive (RIA)      488 490
 Immunochemical blocking agent      595
 Immunocytochemical staining      600 631
 Immunocytochemistry      429
 Immunogen      22 224 236 239 243
 Immunogen conjugation      172
 Immunogen conjugation, using EDC      171
 Immunogen, definition of      419
 Immunogen, gold as carrier      596
 Immunogen, hapten-polymer conjugates      605
 Immunogen, liposome-containing      528 537 550
 Immunogen, preparation of      429
 Immunogenicity      420 421
 Immunogenicity of cBSA      423
 Immunogenicity of cross-linker cross-bridges      229 441
 Immunogenicity of KLH      422
 Immunogenicity of liposomes      550
 Immunogenicity of PEG-modified molecules      607
 Immunogenicity of polymerized haptens      429
 
 | Immunogenicity, modulation by modification with dextran      620 Immunoglobulin      85
 Immunoglobulin binding proteins      456
 Immunoglobulin binding proteins, biotinylated      574
 Immunoglobulin binding proteins, gold-labeled      596 599
 Immunoglobulin binding proteins, immobilized      484
 Immunoglobulin on B lymphocytes      419
 Immunoglobulin, activation with periodate      475
 Immunoglobulin, binding to nickel chelate affinity column      486
 Immunoglobulin, biotinylated      492
 Immunoglobulin, biotinylation of      488
 Immunoglobulin, class      457
 Immunoglobulin, conjugation to liposomes by reductive amination      562
 Immunoglobulin, conjugation, using carbonyl—and sulfhydryl-reactive cross-linkers      249
 Immunoglobulin, directed against immunotoxin conjugates in vivo      496
 Immunoglobulin, FITC-labeled      304
 Immunoglobulin, glycosylation sites      458
 Immunoglobulin, gold labeling of      595 601
 Immunoglobulin, labeled      487
 Immunoglobulin, labeling with AMCA-hydrazide      340
 Immunoglobulin, labeling with biotin-hydrazide      390
 Immunoglobulin, modification with DTPA      366
 Immunoglobulin, modification with sulfo-NHS-biotin      377
 Immunoglobulin, modification, conjugation of, and      456
 Immunoglobulin, optimal fluorophore incorporation      490
 Immunoglobulin, radiolabeling of      488 491
 Immunoglobulin, reduction of      463
 Immunoglobulin, reduction of, using DTT      78
 Immunoglobulin, reduction of, with 2-mercaptoethylamine      82
 Immunoglobulin, structure of      457
 Immunoglobulin, subclasses      463
 Immunoglobulin, thiolation of      461
 Immunoglobulin, thiolation of, using 2-iminothiolane      465
 Immunoglobulin, thiolation of, using PDPH      371
 Immunogold staining      593
 Immunohistochemical staining      83 320 473 478 584 587 633 635
 Immunohistochemical staining, using fluorescently labeled antibodies      489
 Immunotoxins      150 231 233 239 243 252 496 553
 Immunotoxins of protein toxins      497
 Immunotoxins, antibody-to-toxin ratio      503
 Immunotoxins, conjugation using intact A-B toxins      516
 Immunotoxins, conjugation using PDTP      513
 Immunotoxins, conjugation using SPDP      504
 Immunotoxins, cytotoxicity of      501
 Immunotoxins, cytotoxicity relative to cleavable cross-linkers      516
 Immunotoxins, design of      499
 Immunotoxins, gel filtration isolation of      513
 Immunotoxins, general description and use      494
 Immunotoxins, half-life in vivo      511
 Immunotoxins, ideal characteristics      501
 Immunotoxins, immune response toward      496
 Immunotoxins, injection of      495
 Immunotoxins, large-scale preparation of      511
 Immunotoxins, modification with synthetic polymers      496
 Immunotoxins, need for cleavable linker in      499
 Immunotoxins, noncleavable cross-linkers in preparation of      500
 Immunotoxins, nonspecific binding of      501
 Immunotoxins, preparation of      499
 Immunotoxins, preparation of strategies      500
 Immunotoxins, preparation of, using amine—and sulfhydryl—reactive heterobifunctionals      516
 Immunotoxins, preparation of, using aromatic maleimide cross-linkers      522 524
 Immunotoxins, preparation of, using cystamine      514
 Immunotoxins, preparation of, using Ellman’s reagent      515
 Immunotoxins, preparation of, using MBS      522
 Immunotoxins, preparation of, using reductive amination      525
 Immunotoxins, preparation of, using S-sulfonates      516
 Immunotoxins, preparation of, using SIAB      517
 Immunotoxins, preparation of, using SMCC      519
 Immunotoxins, preparation of, using SMPT      511
 Immunotoxins, preparation of, using SPDP      503
 Immunotoxins, preparation of, via disulfide exchange      503
 Immunotoxins, protein toxins used in      497
 Immunotoxins, removal of unconjugated toxin      524
 Inada      606
 Indium-111      365
 Indium-114m      365
 Indium-115      365
 Indium-III      492
 Inesi      310
 Influenza virus      208
 Ingalls      377
 Initiation factor IF2      208
 Inman      112 446 624
 Innis      640
 Insulin      75 86 243 287
 Insulin receptor      260 375
 Insulin, biotinylation of      376
 Insulin-dependent protein kinases      196
 Interferon      196
 Interleukin      2 196
 Interleukin-3 receptor      258
 Intermediate filament      324
 Intraperitoneally      425
 Iodide      403
 Iodination, changes in proteins as a result of      402
 Iodination, controlling the reaction      403
 Iodination, effect on protein activity      402
 Iodination, groups susceptible toward      402
 Iodination, indirect labeling methods      412
 Iodination, methods      401
 Iodination, methods of APDP      277
 Iodination, methods of ASBA      284
 Iodination, methods of ASIB      276
 Iodination, methods of cross-linkers      411
 Iodination, methods of histidine      8 402
 Iodination, methods of NHS-ASA      256
 Iodination, methods of SASD      257
 Iodination, methods of tyrosine      402
 Iodination, oxidizing agents used with      491
 Iodination, process of      401
 Iodination, reactions of      162
 Iodination, reagents      400
 Iodination, scavengers      259
 Iodination, termination of      403
 Iodination, using chloramine-T      403
 Iodination, using IODO-BEADS      405
 Iodination, using IODO-GEN      407
 Iodination, using lactoperoxidase      410
 Iodination, using modification agents      411
 Iodine      162 240 351 360 412 568
 Iodine, radioactive      491
 Iodine, reaction with tyrosine      12
 Iodine, reduction of      403
 Iodine-125      365
 Iodine-131      365
 IODO-BEADS      214 491
 IODO-BEADS, general description and use      405
 IODO-GEN      214 258 401 491
 IODO-GEN for iodination of SASD      259
 IODO-GEN, general description and use      407
 IODO-GEN, iodination protocol      409
 Iodoacetamide      99 131 569
 Iodoacetamide of Lucifer Yellow      359
 Iodoacetamide, blocking unreacted sulfhydryl groups with      519
 Iodoacetate      106
 Iodoacetate for blocking sulfhydryls      131
 Iodoacetate, general description and use      98
 Iodoacetate, modification of amino-dextran with      624
 Iodoacetate, modification of PE      542
 Iodoacetate, relative reactivity of      99 147
 Iodoacetic anhydride, modification of PE      568
 Iodoacetyl      276 307 311 335 350 504 542
 Iodoacetyl in biotinylation reagents      384
 Iodoacetyl of ASIB      276
 Iodoacetyl of benzophenone-4-iodoacetamide      279
 Iodoacetyl of BODIPY 530/550 IA      351
 Iodoacetyl of BODIPY FL IA      350
 Iodoacetyl of DCIA      337
 Iodoacetyl of iodoacetyl-LC-biotin      388
 Iodoacetyl of Lucifer Yellow io-doacetamide      359
 
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