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                    | Hermanson G.T. — Bioconjugate Techniques |  
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                    | Предметный указатель |  
                    | | SPDP, activation of antibodies      509 SPDP, activation of antibody      508
 SPDP, activation of liposomes      547
 SPDP, activation of PE      544
 SPDP, activation of toxins      507
 SPDP, alkaline phosphatase, conjugation to DNA      663
 SPDP, alkaline phosphatase, modified      664
 SPDP, analogs of      65 231
 SPDP, cleavability of      293
 SPDP, comparison to SMPB      242
 SPDP, conjugation of avidin to phycobiliproteins using      588
 SPDP, conjugation of proteins to liposomes using      564
 SPDP, creation using 2,2'-dipyridyl disulfide      133
 SPDP, enzyme activation using      637
 SPDP, general description and use      230
 SPDP, inactivation of gelonin and PAPs      509
 SPDP, liposome conjugation using      542
 SPDP, modification of amine-DNA probes      653
 SPDP, modification of dextran with      624 628
 SPDP, pyridyl disulfide reactions of      151
 SPDP, thiolation of antibodies      518
 SPDP, use for thiolation      64
 Speakman      221
 Speck      407
 Sphingolipids      536 537 539
 Sphingolipids, types      536
 Sphingolipids, use in liposomes      537
 Sphingomyelin      536 537 550
 Sphingosine      534 536 548
 Spiegel      116 121 361
 Srinivasachar      209 211
 Stahlberg      369
 Stanley      195
 Starch      35
 Stark      206
 Staros      140 173 176 178 190 193 194 196 254 438
 Stearic acid      536
 Stearylamine, activation with SPDP      565
 Stearylamine, functional derivatives      539
 Steer      36
 Steric hindrance      306
 Sternberger      584
 Stewart      179 358 359
 Stickel      328
 Stilbenedisulfonate receptor      310
 Stirpe      498
 Stoke’s law      298
 Stoke’s shift      344 351 352 587
 Stoke’s shift of AMCA derivatives      339
 Stoke’s shift of BODIPY 493/503 C3 hydrazide      348
 Stoke’s shift of BODIPY FL C3 hydrazide      349
 Stoke’s shift of BODIPY FL C3-SE      343
 Stoke’s shift of BODIPY fluorophores      342
 Stoke’s shift of Lucifer Yellow fluorophores      359
 Stoke’s shift of phycobiliproteins      362
 Stoke’s shift of tandem phycobiliprotein conjugates      364
 Stoke’s shift, definition of      298
 Straubinger      552
 Streefkerk      472
 Streptavidin      254 287 298 372 378 382 384 386 388 390 492 646
 Streptavidin, activation with bis-hydrazides      222 589
 Streptavidin, activation with sulfo-SMCC      576
 Streptavidin, alkaline phosphatase conjugate      394 661
 Streptavidin, binding to ABNP cross-links      288
 Streptavidin, binding to sulfo-SBED cross-links      289
 Streptavidin, biotin binding site      372
 Streptavidin, comparison to avidin      571
 Streptavidin, conjugation to ferritin using glutaraldehyde      583
 Streptavidin, conjugation to HRP using reductive amination      581 582
 Streptavidin, conjugation to liposomes      554
 Streptavidin, conjugation using glutaraldehyde      583
 Streptavidin, conjugation using reductive amination      580
 Streptavidin, conjugation with enzymes using SMCC      576
 Streptavidin, conjugation with phycoerythrin      363 588
 Streptavidin, effect of periodate      294
 Streptavidin, enzyme-labeled      572 575
 Streptavidin, fluorescent labeling of      584
 Streptavidin, general description and use      570
 Streptavidin, gold-labeled      574 596 602
 Streptavidin, hydrazide-activated      222 574 637
 Streptavidin, immobilized      291 379 382 387
 Streptavidin, interaction with biotin      373
 Streptavidin, interaction with biotinylated antibodies      488 492
 Streptavidin, interaction with iminobiotin      372 380
 Streptavidin, maleimide-activated      578
 Streptavidin, modification with AMCA-NHS      587
 Streptavidin, modification with FITC      585
 Streptavidin, modification with Lissamine rhodamine sulfonyl chloride      586
 Streptavidin, optimal fluorescein labeling level      586
 Streptavidin, preparation of conjugates      575
 Streptavidin, purification using immobilized iminobiotin      381
 Streptavidin, rate of biotin interaction with      372
 Streptavidin, structure and properties      571
 Streptavidin, thiolation, 2-iminothiolane      588
 Streptavidin, thiolation, using SATA      62 579
 Streptavidin, use in assay systems      572
 Streptomyces avidinii      571
 Streptomyces pilosus      370
 Strickland      631
 Strittmatter      532
 Stroh      157
 Strottmann      304
 Stryer      364
 Stuchbury      209
 Stump      599
 Styrene/4-vinylbenzoic acid copolymer      153 183
 Subramanian      365
 Substrates      630
 Subunits, disulfide-linked      9
 Succinaldehyde      215
 Succinic acid      91 94
 Succinic acid in cross-bridge of EGS      199 296
 Succinic anhydride      90 145
 Succinic anhydride, general description and use      91
 Succinic anhydride, modification of amines      93
 Succinic anhydride, modification of mPEG with      615
 Succinic anhydride, modification of PEG with      609 614
 Succinic anhydride, reaction with aminodextran      624
 Succinimidyl 4-(((iodoacetyl)amino) methyl)cyclohexane-1-carboxylate      see “SIAC”
 Succinimidyl 6-((((4-iodoacetyl)amino)methyl)-cyclohexane-1-carbonyl)amino) hexanoate      see “SIACX”
 Succinimidyl 6-((iodoacetyl)-amino)hexanoate      see “SIAX”
 Succinimidyl 6-(6-(((4-iodoacetyl) amino)hexanoyl)amino)hexanoate      see “SIAXX”
 Succinimidyl acetylthiopropionate      see “SATP”
 Succinimidyl carbonate      142 143 156 202
 Succinimidyl carbonate, comparison with CDI coupling      615
 Succinimidyl carbonate, formation of      611 612
 Succinimidyl carbonate, hydrolysis of      611
 Succinimidyl carbonate, reaction with amines      611
 Succinimidyl glutarate      609
 Succinimidyl p-formylbenzoate      see “SFB”
 Succinimidyl p-formylphenoxyacetate      see “SFPA”
 Succinimidyl succinate      609
 Succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate      see “SMCC”
 Succinimidyl-4-(p-maleimidophenyl)butyrate      see “SMPB”
 Succinimidyl-7-amino-4-methylcoumarin-3-acetic acid      see “AMCA-NHS”
 Succinimidyloxycarbonyl-a-methyl-a-(2-pyridyldithio)toluene      see “SMPT”
 Succinimidyl—6—(biotin-amido)hexanoate      see “NHS-LC-biotin”
 Succinylation      91 (see also “Succinic anhydride”)
 Sucrose      35
 Sugars, functional groups      29 (see also “Polysaccharides”)
 Sugars, immobilized      517
 Sugars, labeling with biocytinhydrazide      393
 Sugars, modification with dimines      107
 Sugars, reducing      108 141
 Sulfenylthiosulfate      131 516
 Sulfenylthiosulfate as blocking agent      132
 Sulfhydryl oxidation, metal catalyzed      232
 Sulfhydryls      107
 Sulfhydryls in immobilized reductants      85 87
 Sulfhydryls of 3-galactosidase      460 577
 
 | Sulfhydryls of SAMSA-fluorescein      311 Sulfhydryls of SATA      60
 Sulfhydryls, acetylated      467
 Sulfhydryls, activated with TNB      151
 Sulfhydryls, addition to double bonds      149
 Sulfhydryls, alkylation by maleimides      148
 Sulfhydryls, assay with Ellman’s reagent      88
 Sulfhydryls, biotinylation of      384
 Sulfhydryls, blocking agents, permanent and reversible      126 129 133
 Sulfhydryls, complexation to metals      370
 Sulfhydryls, conjugation with haptens containing      439
 Sulfhydryls, coupling of radionuclides      365
 Sulfhydryls, creation of      231
 Sulfhydryls, creation of AMBH      71
 Sulfhydryls, creation of cystamine      72
 Sulfhydryls, creation of DTT      77
 Sulfhydryls, creation of N-acetyl homocysteine thiolactone      68
 Sulfhydryls, creation of SAMSA      70
 Sulfhydryls, creation of SATA      60
 Sulfhydryls, creation of SATP      63
 Sulfhydryls, creation of SMPT      66
 Sulfhydryls, creation of sodium borohydride      88
 Sulfhydryls, creation of SPDP      64
 Sulfhydryls, creation of, by reduction of disulfides      76
 Sulfhydryls, creation of, by reduction with 2-mercaptoethylamine      82
 Sulfhydryls, creation of, by reduction, using TCEP      83
 Sulfhydryls, creation of, on antibodies      461
 Sulfhydryls, creation of, on antibodies with 2-iminothiolane      465
 Sulfhydryls, creation of, using 2-iminothiolane      57
 Sulfhydryls, dative binding to gold      594
 Sulfhydryls, deprotection of acetylated      578
 Sulfhydryls, detection using Ellman’s reagent      87 132
 Sulfhydryls, formation of pyridyl disulfide groups      133
 Sulfhydryls, introduction of      56
 Sulfhydryls, modification with
  401 Sulfhydryls, modification with 2-bromoethylamine      106
 Sulfhydryls, modification with ethylenimine      105
 Sulfhydryls, permanent and reversible linkages with      208
 Sulfhydryls, preventing oxidation to disulfides      57
 Sulfhydryls, reaction by disulfide exchange      150
 Sulfhydryls, reaction of      11 146
 Sulfhydryls, reaction with
  250 Sulfhydryls, reaction with alkyl halides      209 226
 Sulfhydryls, reaction with aminoethyl-8      104
 Sulfhydryls, reaction with anhydrides      146
 Sulfhydryls, reaction with APDP      277
 Sulfhydryls, reaction with aryl azides      254
 Sulfhydryls, reaction with aryl halides      143 149
 Sulfhydryls, reaction with ASIB      276
 Sulfhydryls, reaction with aziridines      148
 Sulfhydryls, reaction with benzophenone-4-iodoacetamide      279
 Sulfhydryls, reaction with benzophenone-4-maleimide      280
 Sulfhydryls, reaction with biotin-BMCC      384
 Sulfhydryls, reaction with BMH      211
 Sulfhydryls, reaction with CMC      177
 Sulfhydryls, reaction with diazo compounds      153
 Sulfhydryls, reaction with difluorbenzene derivatives      212
 Sulfhydryls, reaction with EDC      172
 Sulfhydryls, reaction with epoxides      142 155 220
 Sulfhydryls, reaction with glutaric anhydride      94
 Sulfhydryls, reaction with GMBS      245
 Sulfhydryls, reaction with haloacetyl compounds      147
 Sulfhydryls, reaction with iodoacetate      98
 Sulfhydryls, reaction with iodoacetyl-LC-biotin      388
 Sulfhydryls, reaction with isothiocyanates      138
 Sulfhydryls, reaction with maleic anhydride      129
 Sulfhydryls, reaction with maleimides      95 209
 Sulfhydryls, reaction with MBS      237
 Sulfhydryls, reaction with MPBH      249
 Sulfhydryls, reaction with NEM      130
 Sulfhydryls, reaction with NHS esters      139 191
 Sulfhydryls, reaction with NPIA      248
 Sulfhydryls, reaction with PDPH      252
 Sulfhydryls, reaction with pyridyl disulfides      151
 Sulfhydryls, reaction with SIAB      239
 Sulfhydryls, reaction with SIAC      247
 Sulfhydryls, reaction with SIAX      245
 Sulfhydryls, reaction with SMCC      235
 Sulfhydryls, reaction with SMPB      242
 Sulfhydryls, reaction with SMPT      233
 Sulfide      632
 Sulfo-BSOCOES, general description and use      199
 Sulfo-DST, general description and use      196
 Sulfo-EGS, general description and use      201
 Sulfo-GMBS, general description and use      245
 Sulfo-GMBS, low immunogenicity of      441
 Sulfo-HSAB, general description and use      260
 Sulfo-HSAB, similarity to sulfo-SAPB      267
 Sulfo-LC-SMPT      66 233
 Sulfo-LC-SPDP      65 231 232
 Sulfo-LC-SPDP in the conjugation of phycobiliproteins      364
 Sulfo-LC-SPDP, activation of liposomes      548
 Sulfo-LC-SPDP, use in protein-liposome conjugation      565
 Sulfo-MBS, general description and use      239
 Sulfo-NHS acetate      127
 Sulfo-NHS esters      101 127 190 235 438
 Sulfo-NHS esters of
  196 Sulfo-NHS esters of DTSSP      193
 Sulfo-NHS esters of SAED      268
 Sulfo-NHS esters of SAND      263
 Sulfo-NHS esters of SASD      256
 Sulfo-NHS esters of sulfo-DST      196
 Sulfo-NHS esters of sulfo-HSAB      260
 Sulfo-NHS esters of sulfo-SADP      266
 Sulfo-NHS esters of sulfo-SAMCA      272
 Sulfo-NHS esters of sulfo-SAPB      266
 Sulfo-NHS esters of sulfo-SBED      289
 Sulfo-NHS esters of sulfo-SHPP      414
 Sulfo-NHS esters of sulfo-SMCC      236
 Sulfo-NHS esters, chemistry of      139
 Sulfo-NHS, produced using the EDC/sulfo-NHS reaction      140 173 430 439
 Sulfo-NHS, use with EDC to activate protein carboxylates      436
 Sulfo-NHS-ASA, general description and use      256
 Sulfo-NHS-biotin, general description and use      376
 Sulfo-NHS-biotin, use in protein biotinylation      377
 Sulfo-NHS-LC-ASA, general description and use      256
 Sulfo-NHS-LC-biotin, general description and use      379
 Sulfo-NHS-SS-biotin, general description and use      382
 Sulfo-SADP, general description and use      266
 Sulfo-SAMCA, general description and use      270
 Sulfo-SANPAH      264
 Sulfo-SANPAH, general description and use      263
 Sulfo-SAPB, general description and use      266
 Sulfo-SBED      291 375
 Sulfo-SBED, functional arms of      291
 Sulfo-SBED, general description and use      289
 Sulfo-SHPP, general description and use      414
 Sulfo-SIAB, general description and use      240
 Sulfo-SMCC      236 441 462 520
 Sulfo-SMCC in hapten-carrier conjugation      439
 Sulfo-SMCC use with poly-L-lysine      428
 Sulfo-SMCC, activation of antibodies using      521
 Sulfo-SMCC, activation of avidin and streptavidin with      576
 Sulfo-SMCC, activation of carrier proteins      444
 Sulfo-SMCC, activation of enzymes with      461 576 636
 Sulfo-SMCC, activation of HRP with      579
 Sulfo-SMCC, general description and use      236
 Sulfo-SMCC, use in antibody-enzyme conjugation      461
 Sulfo-SMPB, general description and use      243
 Sulfonamide linkage      141
 Sulfonate      127 149 190 196 209 236 239 240 243 245 256 260 263 266 267
 Sulfone      213 313
 Sulfone in cleavable reagents      293
 Sulfone, base labile group in cross-linkers      296
 Sulfone, cleavability      198
 Sulfonic acid      140
 Sulfonyl chloride, chemistry of      140
 Sulfonyl of Lissamine rhodamine sulfonyl chloride      323
 Sulfonyl of Texas Red sulfonyl chloride      325
 Sulfonylhydrazine, of rhodamine derivatives      328
 
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