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Hermanson G.T. — Bioconjugate Techniques
Hermanson G.T. — Bioconjugate Techniques



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Название: Bioconjugate Techniques

Автор: Hermanson G.T.

Аннотация:

This is a well-organized and well-written book; it is readable and comprehensible to the novice, but its content and stylistic approach seem sufficiently sophisticated to appeal to active, knowledgeable workers in the field. It is an impressive compilation of useful theoretical and practical information that is not readily available elsewhere in a single volume.


Язык: en

Рубрика: Технология/

Статус предметного указателя: Готов указатель с номерами страниц

ed2k: ed2k stats

Год издания: 1996

Количество страниц: 785

Добавлена в каталог: 15.02.2007

Операции: Положить на полку | Скопировать ссылку для форума | Скопировать ID
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Предметный указатель
SPDP, activation of antibodies      509
SPDP, activation of antibody      508
SPDP, activation of liposomes      547
SPDP, activation of PE      544
SPDP, activation of toxins      507
SPDP, alkaline phosphatase, conjugation to DNA      663
SPDP, alkaline phosphatase, modified      664
SPDP, analogs of      65 231
SPDP, cleavability of      293
SPDP, comparison to SMPB      242
SPDP, conjugation of avidin to phycobiliproteins using      588
SPDP, conjugation of proteins to liposomes using      564
SPDP, creation using 2,2'-dipyridyl disulfide      133
SPDP, enzyme activation using      637
SPDP, general description and use      230
SPDP, inactivation of gelonin and PAPs      509
SPDP, liposome conjugation using      542
SPDP, modification of amine-DNA probes      653
SPDP, modification of dextran with      624 628
SPDP, pyridyl disulfide reactions of      151
SPDP, thiolation of antibodies      518
SPDP, use for thiolation      64
Speakman      221
Speck      407
Sphingolipids      536 537 539
Sphingolipids, types      536
Sphingolipids, use in liposomes      537
Sphingomyelin      536 537 550
Sphingosine      534 536 548
Spiegel      116 121 361
Srinivasachar      209 211
Stahlberg      369
Stanley      195
Starch      35
Stark      206
Staros      140 173 176 178 190 193 194 196 254 438
Stearic acid      536
Stearylamine, activation with SPDP      565
Stearylamine, functional derivatives      539
Steer      36
Steric hindrance      306
Sternberger      584
Stewart      179 358 359
Stickel      328
Stilbenedisulfonate receptor      310
Stirpe      498
Stoke’s law      298
Stoke’s shift      344 351 352 587
Stoke’s shift of AMCA derivatives      339
Stoke’s shift of BODIPY 493/503 C3 hydrazide      348
Stoke’s shift of BODIPY FL C3 hydrazide      349
Stoke’s shift of BODIPY FL C3-SE      343
Stoke’s shift of BODIPY fluorophores      342
Stoke’s shift of Lucifer Yellow fluorophores      359
Stoke’s shift of phycobiliproteins      362
Stoke’s shift of tandem phycobiliprotein conjugates      364
Stoke’s shift, definition of      298
Straubinger      552
Streefkerk      472
Streptavidin      254 287 298 372 378 382 384 386 388 390 492 646
Streptavidin, activation with bis-hydrazides      222 589
Streptavidin, activation with sulfo-SMCC      576
Streptavidin, alkaline phosphatase conjugate      394 661
Streptavidin, binding to ABNP cross-links      288
Streptavidin, binding to sulfo-SBED cross-links      289
Streptavidin, biotin binding site      372
Streptavidin, comparison to avidin      571
Streptavidin, conjugation to ferritin using glutaraldehyde      583
Streptavidin, conjugation to HRP using reductive amination      581 582
Streptavidin, conjugation to liposomes      554
Streptavidin, conjugation using glutaraldehyde      583
Streptavidin, conjugation using reductive amination      580
Streptavidin, conjugation with enzymes using SMCC      576
Streptavidin, conjugation with phycoerythrin      363 588
Streptavidin, effect of periodate      294
Streptavidin, enzyme-labeled      572 575
Streptavidin, fluorescent labeling of      584
Streptavidin, general description and use      570
Streptavidin, gold-labeled      574 596 602
Streptavidin, hydrazide-activated      222 574 637
Streptavidin, immobilized      291 379 382 387
Streptavidin, interaction with biotin      373
Streptavidin, interaction with biotinylated antibodies      488 492
Streptavidin, interaction with iminobiotin      372 380
Streptavidin, maleimide-activated      578
Streptavidin, modification with AMCA-NHS      587
Streptavidin, modification with FITC      585
Streptavidin, modification with Lissamine rhodamine sulfonyl chloride      586
Streptavidin, optimal fluorescein labeling level      586
Streptavidin, preparation of conjugates      575
Streptavidin, purification using immobilized iminobiotin      381
Streptavidin, rate of biotin interaction with      372
Streptavidin, structure and properties      571
Streptavidin, thiolation, 2-iminothiolane      588
Streptavidin, thiolation, using SATA      62 579
Streptavidin, use in assay systems      572
Streptomyces avidinii      571
Streptomyces pilosus      370
Strickland      631
Strittmatter      532
Stroh      157
Strottmann      304
Stryer      364
Stuchbury      209
Stump      599
Styrene/4-vinylbenzoic acid copolymer      153 183
Subramanian      365
Substrates      630
Subunits, disulfide-linked      9
Succinaldehyde      215
Succinic acid      91 94
Succinic acid in cross-bridge of EGS      199 296
Succinic anhydride      90 145
Succinic anhydride, general description and use      91
Succinic anhydride, modification of amines      93
Succinic anhydride, modification of mPEG with      615
Succinic anhydride, modification of PEG with      609 614
Succinic anhydride, reaction with aminodextran      624
Succinimidyl 4-(((iodoacetyl)amino) methyl)cyclohexane-1-carboxylate      see “SIAC”
Succinimidyl 6-((((4-iodoacetyl)amino)methyl)-cyclohexane-1-carbonyl)amino) hexanoate      see “SIACX”
Succinimidyl 6-((iodoacetyl)-amino)hexanoate      see “SIAX”
Succinimidyl 6-(6-(((4-iodoacetyl) amino)hexanoyl)amino)hexanoate      see “SIAXX”
Succinimidyl acetylthiopropionate      see “SATP”
Succinimidyl carbonate      142 143 156 202
Succinimidyl carbonate, comparison with CDI coupling      615
Succinimidyl carbonate, formation of      611 612
Succinimidyl carbonate, hydrolysis of      611
Succinimidyl carbonate, reaction with amines      611
Succinimidyl glutarate      609
Succinimidyl p-formylbenzoate      see “SFB”
Succinimidyl p-formylphenoxyacetate      see “SFPA”
Succinimidyl succinate      609
Succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate      see “SMCC”
Succinimidyl-4-(p-maleimidophenyl)butyrate      see “SMPB”
Succinimidyl-7-amino-4-methylcoumarin-3-acetic acid      see “AMCA-NHS”
Succinimidyloxycarbonyl-a-methyl-a-(2-pyridyldithio)toluene      see “SMPT”
Succinimidyl—6—(biotin-amido)hexanoate      see “NHS-LC-biotin”
Succinylation      91 (see also “Succinic anhydride”)
Sucrose      35
Sugars, functional groups      29 (see also “Polysaccharides”)
Sugars, immobilized      517
Sugars, labeling with biocytinhydrazide      393
Sugars, modification with dimines      107
Sugars, reducing      108 141
Sulfenylthiosulfate      131 516
Sulfenylthiosulfate as blocking agent      132
Sulfhydryl oxidation, metal catalyzed      232
Sulfhydryls      107
Sulfhydryls in immobilized reductants      85 87
Sulfhydryls of 3-galactosidase      460 577
Sulfhydryls of SAMSA-fluorescein      311
Sulfhydryls of SATA      60
Sulfhydryls, acetylated      467
Sulfhydryls, activated with TNB      151
Sulfhydryls, addition to double bonds      149
Sulfhydryls, alkylation by maleimides      148
Sulfhydryls, assay with Ellman’s reagent      88
Sulfhydryls, biotinylation of      384
Sulfhydryls, blocking agents, permanent and reversible      126 129 133
Sulfhydryls, complexation to metals      370
Sulfhydryls, conjugation with haptens containing      439
Sulfhydryls, coupling of radionuclides      365
Sulfhydryls, creation of      231
Sulfhydryls, creation of AMBH      71
Sulfhydryls, creation of cystamine      72
Sulfhydryls, creation of DTT      77
Sulfhydryls, creation of N-acetyl homocysteine thiolactone      68
Sulfhydryls, creation of SAMSA      70
Sulfhydryls, creation of SATA      60
Sulfhydryls, creation of SATP      63
Sulfhydryls, creation of SMPT      66
Sulfhydryls, creation of sodium borohydride      88
Sulfhydryls, creation of SPDP      64
Sulfhydryls, creation of, by reduction of disulfides      76
Sulfhydryls, creation of, by reduction with 2-mercaptoethylamine      82
Sulfhydryls, creation of, by reduction, using TCEP      83
Sulfhydryls, creation of, on antibodies      461
Sulfhydryls, creation of, on antibodies with 2-iminothiolane      465
Sulfhydryls, creation of, using 2-iminothiolane      57
Sulfhydryls, dative binding to gold      594
Sulfhydryls, deprotection of acetylated      578
Sulfhydryls, detection using Ellman’s reagent      87 132
Sulfhydryls, formation of pyridyl disulfide groups      133
Sulfhydryls, introduction of      56
Sulfhydryls, modification with $\mathrm{H_2OI^+}$      401
Sulfhydryls, modification with 2-bromoethylamine      106
Sulfhydryls, modification with ethylenimine      105
Sulfhydryls, permanent and reversible linkages with      208
Sulfhydryls, preventing oxidation to disulfides      57
Sulfhydryls, reaction by disulfide exchange      150
Sulfhydryls, reaction of      11 146
Sulfhydryls, reaction with $\mathrm{M_2C_2H}$      250
Sulfhydryls, reaction with alkyl halides      209 226
Sulfhydryls, reaction with aminoethyl-8      104
Sulfhydryls, reaction with anhydrides      146
Sulfhydryls, reaction with APDP      277
Sulfhydryls, reaction with aryl azides      254
Sulfhydryls, reaction with aryl halides      143 149
Sulfhydryls, reaction with ASIB      276
Sulfhydryls, reaction with aziridines      148
Sulfhydryls, reaction with benzophenone-4-iodoacetamide      279
Sulfhydryls, reaction with benzophenone-4-maleimide      280
Sulfhydryls, reaction with biotin-BMCC      384
Sulfhydryls, reaction with BMH      211
Sulfhydryls, reaction with CMC      177
Sulfhydryls, reaction with diazo compounds      153
Sulfhydryls, reaction with difluorbenzene derivatives      212
Sulfhydryls, reaction with EDC      172
Sulfhydryls, reaction with epoxides      142 155 220
Sulfhydryls, reaction with glutaric anhydride      94
Sulfhydryls, reaction with GMBS      245
Sulfhydryls, reaction with haloacetyl compounds      147
Sulfhydryls, reaction with iodoacetate      98
Sulfhydryls, reaction with iodoacetyl-LC-biotin      388
Sulfhydryls, reaction with isothiocyanates      138
Sulfhydryls, reaction with maleic anhydride      129
Sulfhydryls, reaction with maleimides      95 209
Sulfhydryls, reaction with MBS      237
Sulfhydryls, reaction with MPBH      249
Sulfhydryls, reaction with NEM      130
Sulfhydryls, reaction with NHS esters      139 191
Sulfhydryls, reaction with NPIA      248
Sulfhydryls, reaction with PDPH      252
Sulfhydryls, reaction with pyridyl disulfides      151
Sulfhydryls, reaction with SIAB      239
Sulfhydryls, reaction with SIAC      247
Sulfhydryls, reaction with SIAX      245
Sulfhydryls, reaction with SMCC      235
Sulfhydryls, reaction with SMPB      242
Sulfhydryls, reaction with SMPT      233
Sulfide      632
Sulfo-BSOCOES, general description and use      199
Sulfo-DST, general description and use      196
Sulfo-EGS, general description and use      201
Sulfo-GMBS, general description and use      245
Sulfo-GMBS, low immunogenicity of      441
Sulfo-HSAB, general description and use      260
Sulfo-HSAB, similarity to sulfo-SAPB      267
Sulfo-LC-SMPT      66 233
Sulfo-LC-SPDP      65 231 232
Sulfo-LC-SPDP in the conjugation of phycobiliproteins      364
Sulfo-LC-SPDP, activation of liposomes      548
Sulfo-LC-SPDP, use in protein-liposome conjugation      565
Sulfo-MBS, general description and use      239
Sulfo-NHS acetate      127
Sulfo-NHS esters      101 127 190 235 438
Sulfo-NHS esters of $BS^3$      196
Sulfo-NHS esters of DTSSP      193
Sulfo-NHS esters of SAED      268
Sulfo-NHS esters of SAND      263
Sulfo-NHS esters of SASD      256
Sulfo-NHS esters of sulfo-DST      196
Sulfo-NHS esters of sulfo-HSAB      260
Sulfo-NHS esters of sulfo-SADP      266
Sulfo-NHS esters of sulfo-SAMCA      272
Sulfo-NHS esters of sulfo-SAPB      266
Sulfo-NHS esters of sulfo-SBED      289
Sulfo-NHS esters of sulfo-SHPP      414
Sulfo-NHS esters of sulfo-SMCC      236
Sulfo-NHS esters, chemistry of      139
Sulfo-NHS, produced using the EDC/sulfo-NHS reaction      140 173 430 439
Sulfo-NHS, use with EDC to activate protein carboxylates      436
Sulfo-NHS-ASA, general description and use      256
Sulfo-NHS-biotin, general description and use      376
Sulfo-NHS-biotin, use in protein biotinylation      377
Sulfo-NHS-LC-ASA, general description and use      256
Sulfo-NHS-LC-biotin, general description and use      379
Sulfo-NHS-SS-biotin, general description and use      382
Sulfo-SADP, general description and use      266
Sulfo-SAMCA, general description and use      270
Sulfo-SANPAH      264
Sulfo-SANPAH, general description and use      263
Sulfo-SAPB, general description and use      266
Sulfo-SBED      291 375
Sulfo-SBED, functional arms of      291
Sulfo-SBED, general description and use      289
Sulfo-SHPP, general description and use      414
Sulfo-SIAB, general description and use      240
Sulfo-SMCC      236 441 462 520
Sulfo-SMCC in hapten-carrier conjugation      439
Sulfo-SMCC use with poly-L-lysine      428
Sulfo-SMCC, activation of antibodies using      521
Sulfo-SMCC, activation of avidin and streptavidin with      576
Sulfo-SMCC, activation of carrier proteins      444
Sulfo-SMCC, activation of enzymes with      461 576 636
Sulfo-SMCC, activation of HRP with      579
Sulfo-SMCC, general description and use      236
Sulfo-SMCC, use in antibody-enzyme conjugation      461
Sulfo-SMPB, general description and use      243
Sulfonamide linkage      141
Sulfonate      127 149 190 196 209 236 239 240 243 245 256 260 263 266 267
Sulfone      213 313
Sulfone in cleavable reagents      293
Sulfone, base labile group in cross-linkers      296
Sulfone, cleavability      198
Sulfonic acid      140
Sulfonyl chloride, chemistry of      140
Sulfonyl of Lissamine rhodamine sulfonyl chloride      323
Sulfonyl of Texas Red sulfonyl chloride      325
Sulfonylhydrazine, of rhodamine derivatives      328
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