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                    | Hermanson G.T. — Bioconjugate Techniques |  
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                    | Предметный указатель |  
                    | | N-Acylurea      179 N-Alkyl carbamate      155 183 184 615
 N-Bromosuccinimide      647 648
 N-Bromosuccinimide, reaction with purines      48
 N-Bromosuccinimide, reaction with pyrimidnes      46
 N-Chlorobenzenesulfon-amide      405
 N-Chlorotoluenesulfonamide      see “Chloramine-T”
 N-Ethyl maleimide      see “NEM”
 N-Ethyl-3-phenylisoxa-zolium-3'-sulfonate      see “Woodward’s reagent K”
 N-Formyl peptide receptor      258 263
 N-Glycosidic linkages      6 20 34
 N-Glycosidic linkages in DNA      50
 N-Glycosidic linkages in nucleic acids      41
 N-Glycosidic linkages of purines      47
 N-Haloamine      401 407
 n-Hexadecanoic acid      see “Palmitic acid”
 N-Hydroxysuccinimide      see “NHS”
 N-Hydroxysuccinimido-2-iminobiotin      see “NHS-iminobiotin”
 N-Hydroxysuccinimidyl chloroformate      see “NHS-chloroformate”
 N-Hydroxysuccinimidyl chloroformate, reaction with mPEG      612
 N-hydroxysuccinimidyl-4-azidobenzoate      see “HSAB”
 N-Hydroxysuccinimidyl-4-azidosalicylic acid      see “NHS-ASA”
 N-Hydroxysulfosuccinimide      see “Sulfo-NHS”
 N-Hydroxysulfosuccinimidyl-4-azidobenzoate      see “Sulfo-HSAB”
 N-Iodoacetyl-N-biotinyl-hexylenediamine      see “Iodoacetyl-LC-biotin”
 N-Iodobenzenesulfonamide      406
 N-Maleimido-6-aminocaproyl ester of 1-hydroxy-2-nitro-4-benzene sulfonic acid      618
 n-Octadecanoic acid      see “Stearic acid”
 N-Succinimidyl 3-(2-pyridyldithio)propionate      see “SPDP”
 N-Succinimidyl chloroformate      612
 N-Succinimidyl S-acetylthioacetate      see “SATA”
 N-Succinimidyl-(4-azidophenyl)l,3'-dithiopropionate      see “SADP”
 N-succinimidyl-(4-iodoacetyl)aminobenzoate      see “SIAB”
 N-Succinimidyl-3-(4-hydroxyphenyl)propionate      see “SHPP”
 N-Succinimidyl-6-(4'-azido-2'-nitrophenyl amino)-hexanoate      see “SAN-PAH”
 N-terminal, blocking of      126
 n-Tetradecanoic acid      see “Myristic acid”
 N-[4-(p-Azidosalicylamido)-butyl]-3'-(2'-pyridyldithio) propionamide      see “APDP”
 N-[6-(7-Amino-4-methyl-coumarin-3-acetamido)-hexyl]-3'-(2'-pyridyl-dithio)propionamide      see “AMCA-HPDP”
 N-[6-(biotinamido)-hexyll-3’-(2’-pyridyldithio)-propionamide      see “Biotm-HPDP”
 N1-(p-Isothiocyanatobenzyl) -diethylenetriamine-N1,N2,N3,N3-tetraacetic acid      see “DTTA”
 N4,N4-dimethyladenine      41
 N4-Methoxy-5,6-dihydrocytosine-6-sulphonate      646
 N7-Methylguanine      41
 NAD      19
 NADP      19 208
 Nagai      208
 Nakajima      601
 Nakamura      620
 Nakane      115 472 478
 Nakao      209 211
 NaOH      158
 naphthalene      214
 Nathan      605
 Neisseria gonorrhoeae      205 208
 NEM      130
 NEM, general description and use      130
 Neomycin      270
 Nerve growth factor      207 (see also “NGF”)
 Nerve growth factor, receptor      260
 Neural tracer      354
 Neuraminidase      117 393
 Neuronal tracer      361
 Neurons      322
 Neurons, labeling with biocytin      375
 Neurophysiology      358
 Nevelle      239 522
 Neville      209 211
 Newcastle disease virus      208
 Newhall      205 208
 NGF      252 390
 Ngo      223 286
 NHS      143 156 188 202 245 510
 NHS ester-maleimide cross-linkers, use in avidin conjugation      576
 NHS esters      24 33 123 173 229 461 542 545
 NHS esters in hapten-carrier conjugation      436
 NHS esters of AMCA-NHS      333
 NHS esters of amine reactive biotinylation reagents      373
 NHS esters of ANB-NOS      263
 NHS esters of BODIPY 530/550 C3-SE      344
 NHS esters of BODIPY FL C3-SE      343
 NHS esters of BSOCOES      198
 NHS esters of DSC      156 202
 NHS esters of DSG      201
 NHS esters of DSP      192
 NHS esters of DSS      194
 NHS esters of DST      196
 NHS esters of EGS      199
 NHS esters of GMBS      243
 NHS esters of HSAB      260
 NHS esters of MBS      237 546
 NHS esters of NHS-ASA      255
 NHS esters of NHS-biotin      375
 NHS esters of NHS-chloroformate      156
 NHS esters of NHS-fluorescein      305
 NHS esters of NHS-LC-biotin      377
 NHS esters of NHS-rhodamine      320
 NHS esters of SADP      265
 NHS esters of SANPAH      261
 NHS esters of SATA      467
 NHS esters of SFB      117
 NHS esters of SFPA      117
 NHS esters of SHPP      412
 NHS esters of SIAB      239 517
 NHS esters of SIAC      247
 NHS esters of SIAX      245
 NHS esters of SMCC      235
 NHS esters of SMPB      242
 NHS esters of SMPT      232
 NHS esters of SPDP      230 504
 NHS esters of succinimidyl succinate mPEG      611
 NHS esters of sulfo-NHS-SS-biotin      383
 NHS esters, chemistry of      139
 NHS esters, general description and properties      190
 NHS esters, gold particle containing      597
 NHS esters, half life of      140
 NHS esters, homobifunctional      188
 NHS esters, NHS-aldehydes      186
 NHS esters, similarity to p-nitrophenyl ester      248
 NHS esters, use in PEG activation      609
 NHS, absorptivity of      140 191
 NHS, formation of carboxylate esters      139
 NHS, plus EDC in the formation of active esters      140
 NHS, reaction with mPEG-chloroformate      611
 NHS-ASA, analogs of      256
 NHS-ASA, general description and use      255
 NHS-ASA, iodination of      416
 NHS-biotin reaction with insulin      376
 NHS-biotin, general description and use      375
 NHS-chloroformate      156
 NHS-fluorescein      306
 NHS-fluorescein, general properties and use      305
 NHS-iminobiotin, antibody labeling protocol      382
 NHS-iminobiotin, general description and use      380
 NHS-LC-biotin      651
 NHS-LC-biotin, general description and use      377
 NHS-LC-biotin, modification of amine-DNA probes      658
 NHS-LC-biotin, protein labeling protocol      379
 NHS-LC-biotin, reaction with antibody      492
 NHS-LC-biotin, reaction with PE      554
 NHS-palmitate      557 558
 NHS-rhodamine      322
 NHS-rhodamine, general description and use      320
 NHS-SS-biotin      see “Sulfo-NHS-SS-biotin”
 NHS-SS-biotin, comparison to NHS-LC-biotin      379
 NHS-X-biotin      see “NHS-LC-biotin”
 Nick translation      640
 Nickel ammonium sulfate      486
 Nickel-chelate affinity chromatography      484 486
 Nicknish      173
 
 | Nicolas      46 654 671 Nicolson      601
 Nielsen      600
 Nillson      323 325 473
 Niman      239
 Nissonoff      459
 Nithipatikom      320
 Nitration      110
 Nitrene      163 164 254 261 279 394 395
 Nitrene from phenyl azide photolysis      214
 Nitrene, formation from fluorinated aryl azides      276
 Nitro group, addition to phenolic compounds      110
 Nitrocellulose      379 400
 Noguchi      158 623 624 628
 Norde      595
 Norval      574
 NOTA, general description and use      367
 Novak-Hofer      208
 Novick      196
 NPIA, general description and use      247
 Nucleic acid      101 492
 Nucleic acid probes      321
 Nucleic acid, bromination of      46
 Nucleic acid, chemical modification methods      643
 Nucleic acid, cross-linking methods      55
 Nucleic acid, diamine modification of      644
 Nucleic acid, electrophilic reactions      44
 Nucleic acid, functional groups      41
 Nucleic acid, genetic code      53
 Nucleic acid, labeling with p-aminobenzoyl biocytin, diazotized      398
 Nucleic acid, labeling with photobiotin      395
 Nucleic acid, modification with cystamine      76
 Nucleic acid, modification, conjugation of, and      40 639
 Nucleic acid, nomenclature      41
 Nucleic acid, oligonucleotide structure      53
 Nucleic acid, phosphate groups of      51
 Nucleic acid, polymer linkages      50
 Nucleic acid, purines      47
 Nucleic acid, radiolabeled      663
 Nucleic acid, reaction with CMC      46 177
 Nucleic acid, structure and function      40
 Nucleic acid, succinylation of      93
 Nucleic acid, sugar constituents      41 50
 Nucleophiles      6 42 90
 Nucleophiles, definition      14
 Nucleophiles, reaction with dehydroazepines      214
 Nucleophiles, reaction with epoxides      220
 Nucleophilicity of carboxylates      152
 Nucleophilicity, protonated vs. unprotonated      14
 Nucleophilicity, relative order of      15
 Nucleoproteins      19
 Nucleoside triphosphates      51 640
 Nucleoside triphosphates, labeled      640 644
 Nucleoside, definition of      41
 Nucleotides, definition of      41
 Nucleotides, phosphate groups      51
 Nunez      36
 Nur      646
 O-Acetyltyrosine, breakdown of      127
 O-Acylisourea      123 144 154 170 173 177
 O-Glycosidic linkages      6 20 34
 o-Phthalaldehyde      80 (see also “OPA”)
 o-Tolidine      449
 o-Tolidine in preparing hapten-carrier conjugates      448
 o-Tolidine, diazotized      224 449
 Obrist      494
 Odom      310 313
 Oeltmann      75 505 515
 Oi      363
 Oligomers, formed using homobifunctional cross-linkers      187
 Oligonucleotide      101 109 337
 Oligonucleotide probes, biotinylated      570
 Oligonucleotide synthesis      53
 Oligonucleotide, amine-modified      666
 Oligonucleotide, cross-linking methods      55
 Oligonucleotide, diamine modification of      644
 Oligonucleotide, hydrazide-modified      668
 Oligonucleotide, modification of      40 643
 Oligonucleotide, using biotin-hydrazide      390 661
 Oligonucleotide, using carbodiimides      145
 Oligosaccharides, coupling to carriers using reductive amination      454
 Oligosaccharides, modification with diamines      107
 Oligosaccharides, of gangliosides      548
 Olin      486
 Olive      338
 Olofson      181
 Olsnes      497 499 525
 Olson      291 532
 Ondetti      177
 OPA      114
 OPA for the detection of amine compounds      82 113
 Oparka      355
 Opioid receptors      196 198
 Order      365 490 494
 Orgel      653
 Orlandi      496
 Ormerod      489
 Orr      380
 Orthophosphoric monoester phosphorylase      see “Alkaline phosphatase”
 Osazone      32
 Osborn      489
 Osmotic shock      359
 Osone      32
 Otsuka      365
 Ottenbrite      606
 Ottesen      208
 OVA      436
 OVA, activation with sulfo-SMCC      441
 OVA, conjugated to cBSA      425
 OVA, conjugation with [Met5]-enkephalin using EDC      433
 OVA, functional groups of      426
 OVA, solubility in the presence of DMSO      427
 OVA, use as carrier      426
 Ovalbumin      see “OVA”
 Overbeek      594
 Oxidants, immobilized      403
 Oxidases      108 186 524
 Oxirane, chemistry of      142
 Oxirane, reactions of      155
 Oxoaldonic acid      29
 Ozawa      209 226 504
 O’Dorisio      196 201 260 263
 O’Hare      500
 O’Keefe      205
 O’Malley      634
 O’Shannessy      222 283 390
 O’Sullivan      210 239
 p-Aminobenzoyl biocytin      659
 p-Aminobenzoyl biocytin, diazotization of      399
 p-Aminobenzoyl biocytin, general description and use      398
 p-Aminobenzoyl biocytin, modification of DNA with diazo derivative      658
 p-Azidobenzoyl hydrazide      see “ABH”
 p-Azidophenyl glyoxal      see “APG”
 p-Diaminodiphenyl      see “Ben-zidine”
 p-Hydroxyphenylacetic acid, use as an iodinarion scavenger      259
 p-Maleimidophenyl isocyanate      see “PMPI”
 p-Nitrophenol      274
 p-Nitrophenol, reduction of      110
 p-Nitrophenyl -2-diazo-3,3,3-trifluoropropionate      see “PNP-DTP”
 p-Nitrophenyl diazopyruvate      186
 p-Nitrophenyl diazopyruvate, general description and use      272
 p-Nitrophenyl ester      375
 p-Nitrophenyl ester of ABNP      287
 p-Nitrophenyl ester of NPIA      247
 p-Nitrophenyl ester of p-nitrophenyl diazopyruvate      272
 p-Nitrophenyl ester of PNP-DTP      274
 p-Nitrophenyl iodoacetate      see “NPIA”
 p-Nitrophenylglyoxal, reaction with arginine      284
 Packman      207
 Paganelli      574
 
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