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Hermanson G.T. — Bioconjugate Techniques
Hermanson G.T. — Bioconjugate Techniques



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Название: Bioconjugate Techniques

Автор: Hermanson G.T.

Аннотация:

This is a well-organized and well-written book; it is readable and comprehensible to the novice, but its content and stylistic approach seem sufficiently sophisticated to appeal to active, knowledgeable workers in the field. It is an impressive compilation of useful theoretical and practical information that is not readily available elsewhere in a single volume.


Язык: en

Рубрика: Технология/

Статус предметного указателя: Готов указатель с номерами страниц

ed2k: ed2k stats

Год издания: 1996

Количество страниц: 785

Добавлена в каталог: 15.02.2007

Операции: Положить на полку | Скопировать ссылку для форума | Скопировать ID
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Предметный указатель
Alkyl halide, reactions of      147 158
Alkyl halide, reactions with pyrimidines      44
Alkylation      8
Alkylation by maleimides      95
Alkylation of amines      137
Alkylation of hydroxyls      29
Alkylation of nucleic acids      44
Alkylation of purines      48
Alkylation of pyrimidines      44
Alkylation of sulfhydryls      146
Alkylation with iodoacetate      98
Alkylation, reductive      160 185;
Allen      537
Allergic reactions, to immunotoxins      496
Allfrey      209
Allison      131 428 533 549
Allmer      223
Allophycocyanin      363
Allophycocyanin, tandem conjugate with B-phycoerythrin      364
Alum      425
Alvear      88
Alving      428 533 549
Amadori rearrangement      37 141
AMBH      252
AMBH, modification of oxidized carbohydrates      71
AMBH, use for thiolation      71
AMCA      331 332 488
AMCA, as photoreactive group in SAED      268
AMCA, comparison to fluorescein      332
AMCA, general description and use      332
AMCA, group in sulfo-SAMCA      272
AMCA, hydrazide derivative of      339
AMCA, labeling of avidin with      587
AMCA, reaction with EDC      332
AMCA, sulfhydryl-reactive derivatives      335
AMCA, within SAED      299
AMCA-HPDP      651
AMCA-HPDP, general properties and use      335
AMCA-HPDP, protein modification protocol      337
AMCA-hydrazide, general properties and use      339
AMCA-hydrazide, modification of antibodies      341
AMCA-NHS      587
AMCA-NHS, general properties and use      333
AMCA-NHS, labeling of avidin with      587
AMCA-NHS, photodecomposition of      333
AMCA-NHS, protein labeling protocol      335
AMCA-sulfo-NHS, general properties and use      333
Amidine      144 203 204 205 206 207 562
Amine boranes      142
Amines, $\alpha$-amines of biocytin      392
Amines, $\alpha$-amines, pKa      7
Amines, $\varepsilon$-amines      7 91
Amines, acylation with citraconic anhydride      96
Amines, acylation with glutaric anhydride      94
Amines, acylation with maleic anhydride      94
Amines, acylation with succinic anhydride      91
Amines, blocking of      125 126
Amines, blocking with acetic anhydride      127
Amines, blocking with sulfo-NHS-acetate      127
Amines, coupling by reductive animation      185
Amines, coupling using CMC      177
Amines, coupling using DCC      178
Amines, coupling using DIG      181
Amines, coupling using EDC      170
Amines, coupling with carbodiimides      144
Amines, coupling with Woodward's reagent K      181
Amines, detection of      112
Amines, detection with TNBS      112
Amines, detection, using OPA      80 113
Amines, for blocking aldehydes      135
Amines, introduction of      100
Amines, Mannich reaction with formaldehyde      216
Amines, modification with glutaraldehyde      118
Amines, modification with SFB      117
Amines, modification with SFPA      117
Amines, reaction of      137
Amines, reaction with acyl azides      139
Amines, reaction with aldehydes      141
Amines, reaction with alkyl halides      226
Amines, reaction with ANB-NOS      263
Amines, reaction with anhydrides      146
Amines, reaction with aryl halides      143 149
Amines, reaction with carbodiimides      154
Amines, reaction with chloroacetic acid      100
Amines, reaction with dehydroazepines      254
Amines, reaction with diazopyruvates      272
Amines, reaction with difluorobenzene derivatives      211
Amines, reaction with epoxides      142 155 220
Amines, reaction with formaldehyde      216
Amines, reaction with GDI-activated hydroxyls      155
Amines, reaction with glutaraldehyde      218
Amines, reaction with GMBS      244
Amines, reaction with haloacetyl compounds      147
Amines, reaction with HSAB      260
Amines, reaction with imidoesters      143 203
Amines, reaction with isocyanates      138
Amines, reaction with isothiocyanates      138
Amines, reaction with maleimides      148
Amines, reaction with MBS      237
Amines, reaction with NHS esters      139 191
Amines, reaction with NHS-ASA      256
Amines, reaction with NHS-chloroformate activated hydroxyls      156
Amines, reaction with NPIA      248
Amines, reaction with p-nitrophenyl diazopyruvate      272
Amines, reaction with p-nitrophenyl esters      248
Amines, reaction with PNP-DTP      274
Amines, reaction with SADP      265
Amines, reaction with SAED      268
Amines, reaction with SAND      264
Amines, reaction with SANPAH      261
Amines, reaction with SASD      257
Amines, reaction with SIAB      239
Amines, reaction with SIAC      247
Amines, reaction with SIAX      245
Amines, reaction with SMCC      235
Amines, reaction with SMPB      242
Amines, reaction with SMPT      233
Amines, reaction with SPDP      231
Amines, reaction with sulfo-SAMCA      272
Amines, reaction with sulfo-SAPB      266
Amines, reaction with sulfo-SBED      289
Amines, reaction with sulfonyl chlorides      140
Amines, reversible blocking with maleic anhydride      129
Amines, Schiff base formation      160
Amino acid      5
Amino acid analysis      308
Amino acid, aliphatic      5
Amino acid, aromatic, hydrophobic      6
Amino acid, detection with OPA      113
Amino acid, ionizable      6
Amino acid, polar, hydrophilic      6
Aminoacyl-transferase II      see “EF2”
Aminoalkylation      101
Aminoethyl-8      105
Aminoethyl-8, general description and use      104
Aminoethylation      104
Aminoethylation of cBSA      423
Aminoethylthioacetyl spacer      542
Ammonia      32 148 216 450
Ammonia in Mannich reaction      161
Ammonia in reductive amination      160
Ammonia, coupling to aldehydes      109
Ammonium chloride      305 320
Ammonium ion, reaction with an epoxide      142
AMP, immobilized      647
Amphipathic      533 536 548
Amphipathic chelating molecules      368
Amphiphilic      528 606
Amylopectin      620
ANB-NOS, general description and use      263
Anderson      39 133 153 183 454 615
Ando      308 309
Angiotensin II      195
Anhydrides      90 128 129
Anhydrides of DTPA      365 492
Anhydrides of SAMSA      70
Anhydrides, as blocking agents      126
Anhydrides, formation using DCC      180
Anhydrides, glutaric anhydride      94
Anhydrides, maleic anhydride      94
Anhydrides, modification of amines      90
Anhydrides, modification of PEG with      609
Anhydrides, reactions of      145
Anhydrides, succinic      91
Aniline      32
Aniline, derivatives      402
Anjaneyulu      173 176 190 438
Annunziato      138 158 159
Anterograde tracing studies      375 620
Antibodies      22 78 102 125 223 224 236 241 252 258
Antibodies as a targeting component      630
Antibodies at sulfhydryl sites      384
Antibodies, $\beta$-galactosidase conjugate      634
Antibodies, activation of, with PDTP      513
Antibodies, activation of, with periodate      475 477
Antibodies, activation of, with SMCC      501 521
Antibodies, activation of, with SPDP      504 508 509
Antibodies, activation of, with sulfo-SMCC      461 520
Antibodies, biotinylated      363 372 476 492 572 574 584 638
Antibodies, biotinylation of      488
Antibodies, carrier-specific      426
Antibodies, class      457
Antibodies, conjugation of, controlling the size of conjugates      473
Antibodies, conjugation of, dextran conjugate with toxins      494
Antibodies, conjugation of, through carbohydrate regions      459
Antibodies, conjugation of, to liposomes      552
Antibodies, conjugation of, to liposomes, using NHS-palmitate      556
Antibodies, conjugation of, to toxins, using cystamine      515
Antibodies, conjugation of, to toxins, using SIAB      517
Antibodies, conjugation of, to toxins, using SMPT      511
Antibodies, conjugation of, to toxins, using via disulfide exchange      478 503
Antibodies, conjugation of, using antibody fragments      478
Antibodies, conjugation of, using glutaraldehyde      470
Antibodies, conjugation of, using reduced antibodies      463
Antibodies, conjugation of, with amine or hydrazide derivatives      476
Antibodies, conjugation of, with deglycosylated ricin A chains      501
Antibodies, conjugation of, with dextran      624
Antibodies, conjugation of, with enzymes      460
Antibodies, conjugation of, with glucose oxidase      635
Antibodies, conjugation of, with intact toxins      494 499 501
Antibodies, conjugation of, with lymphokines      494
Antibodies, conjugation of, with periodate-oxidized HRP      115
Antibodies, conjugation of, with phycobiliproteins      363
Antibodies, conjugation of, with ricin A chain using SMPT      511
Antibodies, conjugation of, with SMCC-activated enzymes      461
Antibodies, conjugation of, with toxins using oxidized dextran      494 527
Antibodies, cross-linking with DSP      193
Antibodies, cytotoxic complexes      494
Antibodies, detection using biotinylated immunoglobulin binding proteins      574
Antibodies, detection using protein A-gold      599
Antibodies, digestion with papain      480
Antibodies, digestion with pepsin      479
Antibodies, directed against the crossbridge      229
Antibodies, EDC conjugation to liposomes      559
Antibodies, effect of sodium cyanoborohydride      186
Antibodies, enzymatic digestion of      458
Antibodies, enzyme conjugates of      636
Antibodies, enzyme conjugation with DPDPB      210
Antibodies, fluorescein-labeled      304 588
Antibodies, fluorescently labeled      297 488
Antibodies, functioning as the B chain in immunotoxins      499
Antibodies, glucose oxidase conjugate      635
Antibodies, glycosylation sites      458
Antibodies, gold labeling of      600
Antibodies, gold-labeled      596
Antibodies, humanized mouse monoclonals      496
Antibodies, immobilization using the protein A/DMP method      206
Antibodies, immunotoxin conjugate design      499
Antibodies, labeling with AMCA-hydrazide      340
Antibodies, labeling with NHS-fluorescein      307
Antibodies, labeling with NHS-rhodamine      322
Antibodies, labeling with radionuclides      365 492
Antibodies, labeling with TRITC      320
Antibodies, liposome conjugate with      494 528
Antibodies, modification, conjugation of, and      456
Antibodies, modification, with $^{99m}Tc}$ through sulf-hydryls      370
Antibodies, modification, with bifunctional chelating agents      365 492
Antibodies, modification, with biotin-HPDP      387
Antibodies, modification, with chelating polymer      366
Antibodies, modification, with DFA      370
Antibodies, modification, with DTPA      366
Antibodies, modification, with DTTA      369
Antibodies, modification, with fluorescein-5-thiosemicar-bazide      313
Antibodies, modification, with NHS-iminobiotin      380 381
Antibodies, modification, with NHS-LC-biotin      379 492
Antibodies, modification, with sulfo-NHS-SS-biotin      382
Antibodies, monoclonal      365 494
Antibodies, optimal fluorophore incorporation      489
Antibodies, partial reduction using DTT      522 523
Antibodies, periodate oxidation of      475 477
Antibodies, pI of      595
Antibodies, preserving antigen binding sites      21 228
Antibodies, primary      489
Antibodies, production of      419
Antibodies, radiolabeled      298 365 460
Antibodies, radiolabeling of      488 490
Antibodies, reactions with sodium cyanoborohydride      142
Antibodies, recombinant      460 496
Antibodies, reduction of disulfides      76
Antibodies, reduction of disulfides, using DTT      523
Antibodies, reduction of disulfides, with 2-mercaptoethyl-amine      82 336
Antibodies, reductive amination with enzymes      476
Antibodies, removal of unconjugated enzyme      483
Antibodies, role of in immunotoxins      499
Antibodies, SATA-modified      469
Antibodies, secondary      489
Antibodies, single-chain      496
Antibodies, site-directed conjugation of      459
Antibodies, SMCC-activated      501
Antibodies, structure of      457
Antibodies, thiolation of      461
Antibodies, thiolation of, at carbohydrate residues      371
Antibodies, thiolation of, with 2-iminothiolane      465
Antibodies, thiolation of, with PDPH      371
Antibodies, thiolation of, with SATA      61 467
Antibodies, thiolation of, with SPDP      504 508 518
Antibody immunoconjugates for cancer      494
Antibody response against a cross-linker      429
Antibody response, to liposome immunogens      549
Antibody response, toward cross-linker      441
Antibody response, toward polymerized haptens      429
Antibody-deglycosylated ricin A chain, largescale preparation of      511
Antibody-enzyme conjugation      120 188 220 235 236 239 241
Antibody-enzyme conjugation, purification by immunoaffinity chromatography      485
Antibody-enzyme conjugation, removal of unconjugated enzyme      484
Antibody-enzyme conjugation, using metal chelate affinity chromatography      486
Antibody-enzyme conjugation, using NHS ester-maleimide cross-linkers      461
Antibody-liposome conjugates      552
Antibody-toxin conjugates, formation via pyridyl disulfide cross-linkers      503
Antigen      387 488 493 554
Antigen binding sites      78 82 249 370 371 458 465 476 482 483 485 490 496 502 636
Antigen binding sites, blocking of      456 459
Antigen presenting cells      423
Antigen specificity      365
Antigen, definition of      419
Antigen, detection using biotinylated antibodies      584
Antigen, detection using colloidal gold labeling      593
Antigen, encapsulation in liposomes      550
Antigen, immobilized      456 484
Antigen, localized using avidin-biotin interaction      574
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