| 
		        
			        |  |  
			        |  |  
					| Авторизация |  
					|  |  
			        |  |  
			        | Поиск по указателям |  
			        | 
 |  
			        |  |  
			        |  |  
			        |  |  
                    |  |  
			        |  |  
			        |  |  |  | 
		|  |  
                    | Hermanson G.T. — Bioconjugate Techniques |  
                    |  |  
			        |  |  
                    | Предметный указатель |  
                    | | Alkyl halide, reactions of      147 158 Alkyl halide, reactions with pyrimidines      44
 Alkylation      8
 Alkylation by maleimides      95
 Alkylation of amines      137
 Alkylation of hydroxyls      29
 Alkylation of nucleic acids      44
 Alkylation of purines      48
 Alkylation of pyrimidines      44
 Alkylation of sulfhydryls      146
 Alkylation with iodoacetate      98
 Alkylation, reductive      160 185;
 Allen      537
 Allergic reactions, to immunotoxins      496
 Allfrey      209
 Allison      131 428 533 549
 Allmer      223
 Allophycocyanin      363
 Allophycocyanin, tandem conjugate with B-phycoerythrin      364
 Alum      425
 Alvear      88
 Alving      428 533 549
 Amadori rearrangement      37 141
 AMBH      252
 AMBH, modification of oxidized carbohydrates      71
 AMBH, use for thiolation      71
 AMCA      331 332 488
 AMCA, as photoreactive group in SAED      268
 AMCA, comparison to fluorescein      332
 AMCA, general description and use      332
 AMCA, group in sulfo-SAMCA      272
 AMCA, hydrazide derivative of      339
 AMCA, labeling of avidin with      587
 AMCA, reaction with EDC      332
 AMCA, sulfhydryl-reactive derivatives      335
 AMCA, within SAED      299
 AMCA-HPDP      651
 AMCA-HPDP, general properties and use      335
 AMCA-HPDP, protein modification protocol      337
 AMCA-hydrazide, general properties and use      339
 AMCA-hydrazide, modification of antibodies      341
 AMCA-NHS      587
 AMCA-NHS, general properties and use      333
 AMCA-NHS, labeling of avidin with      587
 AMCA-NHS, photodecomposition of      333
 AMCA-NHS, protein labeling protocol      335
 AMCA-sulfo-NHS, general properties and use      333
 Amidine      144 203 204 205 206 207 562
 Amine boranes      142
 Amines,
  -amines of biocytin      392 Amines,
  -amines, pKa      7 Amines,
  -amines      7 91 Amines, acylation with citraconic anhydride      96
 Amines, acylation with glutaric anhydride      94
 Amines, acylation with maleic anhydride      94
 Amines, acylation with succinic anhydride      91
 Amines, blocking of      125 126
 Amines, blocking with acetic anhydride      127
 Amines, blocking with sulfo-NHS-acetate      127
 Amines, coupling by reductive animation      185
 Amines, coupling using CMC      177
 Amines, coupling using DCC      178
 Amines, coupling using DIG      181
 Amines, coupling using EDC      170
 Amines, coupling with carbodiimides      144
 Amines, coupling with Woodward's reagent K      181
 Amines, detection of      112
 Amines, detection with TNBS      112
 Amines, detection, using OPA      80 113
 Amines, for blocking aldehydes      135
 Amines, introduction of      100
 Amines, Mannich reaction with formaldehyde      216
 Amines, modification with glutaraldehyde      118
 Amines, modification with SFB      117
 Amines, modification with SFPA      117
 Amines, reaction of      137
 Amines, reaction with acyl azides      139
 Amines, reaction with aldehydes      141
 Amines, reaction with alkyl halides      226
 Amines, reaction with ANB-NOS      263
 Amines, reaction with anhydrides      146
 Amines, reaction with aryl halides      143 149
 Amines, reaction with carbodiimides      154
 Amines, reaction with chloroacetic acid      100
 Amines, reaction with dehydroazepines      254
 Amines, reaction with diazopyruvates      272
 Amines, reaction with difluorobenzene derivatives      211
 Amines, reaction with epoxides      142 155 220
 Amines, reaction with formaldehyde      216
 Amines, reaction with GDI-activated hydroxyls      155
 Amines, reaction with glutaraldehyde      218
 Amines, reaction with GMBS      244
 Amines, reaction with haloacetyl compounds      147
 Amines, reaction with HSAB      260
 Amines, reaction with imidoesters      143 203
 Amines, reaction with isocyanates      138
 Amines, reaction with isothiocyanates      138
 Amines, reaction with maleimides      148
 Amines, reaction with MBS      237
 Amines, reaction with NHS esters      139 191
 Amines, reaction with NHS-ASA      256
 Amines, reaction with NHS-chloroformate activated hydroxyls      156
 Amines, reaction with NPIA      248
 Amines, reaction with p-nitrophenyl diazopyruvate      272
 Amines, reaction with p-nitrophenyl esters      248
 Amines, reaction with PNP-DTP      274
 Amines, reaction with SADP      265
 Amines, reaction with SAED      268
 Amines, reaction with SAND      264
 Amines, reaction with SANPAH      261
 Amines, reaction with SASD      257
 Amines, reaction with SIAB      239
 Amines, reaction with SIAC      247
 Amines, reaction with SIAX      245
 Amines, reaction with SMCC      235
 Amines, reaction with SMPB      242
 Amines, reaction with SMPT      233
 Amines, reaction with SPDP      231
 Amines, reaction with sulfo-SAMCA      272
 Amines, reaction with sulfo-SAPB      266
 Amines, reaction with sulfo-SBED      289
 Amines, reaction with sulfonyl chlorides      140
 Amines, reversible blocking with maleic anhydride      129
 Amines, Schiff base formation      160
 Amino acid      5
 Amino acid analysis      308
 Amino acid, aliphatic      5
 Amino acid, aromatic, hydrophobic      6
 Amino acid, detection with OPA      113
 Amino acid, ionizable      6
 Amino acid, polar, hydrophilic      6
 Aminoacyl-transferase II      see “EF2”
 Aminoalkylation      101
 Aminoethyl-8      105
 Aminoethyl-8, general description and use      104
 Aminoethylation      104
 Aminoethylation of cBSA      423
 Aminoethylthioacetyl spacer      542
 Ammonia      32 148 216 450
 Ammonia in Mannich reaction      161
 Ammonia in reductive amination      160
 Ammonia, coupling to aldehydes      109
 Ammonium chloride      305 320
 Ammonium ion, reaction with an epoxide      142
 AMP, immobilized      647
 Amphipathic      533 536 548
 Amphipathic chelating molecules      368
 Amphiphilic      528 606
 Amylopectin      620
 ANB-NOS, general description and use      263
 
 | Anderson      39 133 153 183 454 615 Ando      308 309
 Angiotensin II      195
 Anhydrides      90 128 129
 Anhydrides of DTPA      365 492
 Anhydrides of SAMSA      70
 Anhydrides, as blocking agents      126
 Anhydrides, formation using DCC      180
 Anhydrides, glutaric anhydride      94
 Anhydrides, maleic anhydride      94
 Anhydrides, modification of amines      90
 Anhydrides, modification of PEG with      609
 Anhydrides, reactions of      145
 Anhydrides, succinic      91
 Aniline      32
 Aniline, derivatives      402
 Anjaneyulu      173 176 190 438
 Annunziato      138 158 159
 Anterograde tracing studies      375 620
 Antibodies      22 78 102 125 223 224 236 241 252 258
 Antibodies as a targeting component      630
 Antibodies at sulfhydryl sites      384
 Antibodies,
  -galactosidase conjugate      634 Antibodies, activation of, with PDTP      513
 Antibodies, activation of, with periodate      475 477
 Antibodies, activation of, with SMCC      501 521
 Antibodies, activation of, with SPDP      504 508 509
 Antibodies, activation of, with sulfo-SMCC      461 520
 Antibodies, biotinylated      363 372 476 492 572 574 584 638
 Antibodies, biotinylation of      488
 Antibodies, carrier-specific      426
 Antibodies, class      457
 Antibodies, conjugation of, controlling the size of conjugates      473
 Antibodies, conjugation of, dextran conjugate with toxins      494
 Antibodies, conjugation of, through carbohydrate regions      459
 Antibodies, conjugation of, to liposomes      552
 Antibodies, conjugation of, to liposomes, using NHS-palmitate      556
 Antibodies, conjugation of, to toxins, using cystamine      515
 Antibodies, conjugation of, to toxins, using SIAB      517
 Antibodies, conjugation of, to toxins, using SMPT      511
 Antibodies, conjugation of, to toxins, using via disulfide exchange      478 503
 Antibodies, conjugation of, using antibody fragments      478
 Antibodies, conjugation of, using glutaraldehyde      470
 Antibodies, conjugation of, using reduced antibodies      463
 Antibodies, conjugation of, with amine or hydrazide derivatives      476
 Antibodies, conjugation of, with deglycosylated ricin A chains      501
 Antibodies, conjugation of, with dextran      624
 Antibodies, conjugation of, with enzymes      460
 Antibodies, conjugation of, with glucose oxidase      635
 Antibodies, conjugation of, with intact toxins      494 499 501
 Antibodies, conjugation of, with lymphokines      494
 Antibodies, conjugation of, with periodate-oxidized HRP      115
 Antibodies, conjugation of, with phycobiliproteins      363
 Antibodies, conjugation of, with ricin A chain using SMPT      511
 Antibodies, conjugation of, with SMCC-activated enzymes      461
 Antibodies, conjugation of, with toxins using oxidized dextran      494 527
 Antibodies, cross-linking with DSP      193
 Antibodies, cytotoxic complexes      494
 Antibodies, detection using biotinylated immunoglobulin binding proteins      574
 Antibodies, detection using protein A-gold      599
 Antibodies, digestion with papain      480
 Antibodies, digestion with pepsin      479
 Antibodies, directed against the crossbridge      229
 Antibodies, EDC conjugation to liposomes      559
 Antibodies, effect of sodium cyanoborohydride      186
 Antibodies, enzymatic digestion of      458
 Antibodies, enzyme conjugates of      636
 Antibodies, enzyme conjugation with DPDPB      210
 Antibodies, fluorescein-labeled      304 588
 Antibodies, fluorescently labeled      297 488
 Antibodies, functioning as the B chain in immunotoxins      499
 Antibodies, glucose oxidase conjugate      635
 Antibodies, glycosylation sites      458
 Antibodies, gold labeling of      600
 Antibodies, gold-labeled      596
 Antibodies, humanized mouse monoclonals      496
 Antibodies, immobilization using the protein A/DMP method      206
 Antibodies, immunotoxin conjugate design      499
 Antibodies, labeling with AMCA-hydrazide      340
 Antibodies, labeling with NHS-fluorescein      307
 Antibodies, labeling with NHS-rhodamine      322
 Antibodies, labeling with radionuclides      365 492
 Antibodies, labeling with TRITC      320
 Antibodies, liposome conjugate with      494 528
 Antibodies, modification, conjugation of, and      456
 Antibodies, modification, with
  through sulf-hydryls      370 Antibodies, modification, with bifunctional chelating agents      365 492
 Antibodies, modification, with biotin-HPDP      387
 Antibodies, modification, with chelating polymer      366
 Antibodies, modification, with DFA      370
 Antibodies, modification, with DTPA      366
 Antibodies, modification, with DTTA      369
 Antibodies, modification, with fluorescein-5-thiosemicar-bazide      313
 Antibodies, modification, with NHS-iminobiotin      380 381
 Antibodies, modification, with NHS-LC-biotin      379 492
 Antibodies, modification, with sulfo-NHS-SS-biotin      382
 Antibodies, monoclonal      365 494
 Antibodies, optimal fluorophore incorporation      489
 Antibodies, partial reduction using DTT      522 523
 Antibodies, periodate oxidation of      475 477
 Antibodies, pI of      595
 Antibodies, preserving antigen binding sites      21 228
 Antibodies, primary      489
 Antibodies, production of      419
 Antibodies, radiolabeled      298 365 460
 Antibodies, radiolabeling of      488 490
 Antibodies, reactions with sodium cyanoborohydride      142
 Antibodies, recombinant      460 496
 Antibodies, reduction of disulfides      76
 Antibodies, reduction of disulfides, using DTT      523
 Antibodies, reduction of disulfides, with 2-mercaptoethyl-amine      82 336
 Antibodies, reductive amination with enzymes      476
 Antibodies, removal of unconjugated enzyme      483
 Antibodies, role of in immunotoxins      499
 Antibodies, SATA-modified      469
 Antibodies, secondary      489
 Antibodies, single-chain      496
 Antibodies, site-directed conjugation of      459
 Antibodies, SMCC-activated      501
 Antibodies, structure of      457
 Antibodies, thiolation of      461
 Antibodies, thiolation of, at carbohydrate residues      371
 Antibodies, thiolation of, with 2-iminothiolane      465
 Antibodies, thiolation of, with PDPH      371
 Antibodies, thiolation of, with SATA      61 467
 Antibodies, thiolation of, with SPDP      504 508 518
 Antibody immunoconjugates for cancer      494
 Antibody response against a cross-linker      429
 Antibody response, to liposome immunogens      549
 Antibody response, toward cross-linker      441
 Antibody response, toward polymerized haptens      429
 Antibody-deglycosylated ricin A chain, largescale preparation of      511
 Antibody-enzyme conjugation      120 188 220 235 236 239 241
 Antibody-enzyme conjugation, purification by immunoaffinity chromatography      485
 Antibody-enzyme conjugation, removal of unconjugated enzyme      484
 Antibody-enzyme conjugation, using metal chelate affinity chromatography      486
 Antibody-enzyme conjugation, using NHS ester-maleimide cross-linkers      461
 Antibody-liposome conjugates      552
 Antibody-toxin conjugates, formation via pyridyl disulfide cross-linkers      503
 Antigen      387 488 493 554
 Antigen binding sites      78 82 249 370 371 458 465 476 482 483 485 490 496 502 636
 Antigen binding sites, blocking of      456 459
 Antigen presenting cells      423
 Antigen specificity      365
 Antigen, definition of      419
 Antigen, detection using biotinylated antibodies      584
 Antigen, detection using colloidal gold labeling      593
 Antigen, encapsulation in liposomes      550
 Antigen, immobilized      456 484
 Antigen, localized using avidin-biotin interaction      574
 
 | 
 |  |  |  | Реклама |  |  |  |  |  |