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Lakowicz J.R. Ч Principles of Fluorescence Spectroscopy
Lakowicz J.R. Ч Principles of Fluorescence Spectroscopy

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Ќазвание: Principles of Fluorescence Spectroscopy

јвтор: Lakowicz J.R.

јннотаци€:

In the second edition of Principles I have attempted to maintain the emphasis on basics, while updating the examples to include more recent results from the literature. There is a new chapter providing an overview of extrinisic fluorophores. The discussion of timeresolved measurements has been expanded to two chapters. Quenching has also been expanded in two chapters. Energy transfer and anisotropy have each been expanded to three chapters. There is also a new chapter on fluorescence sensing. To enhance the usefulness of this book as a textbook, most chapters are followed by a set of problems. Sections which describe advanced topics are indicated as such, to allow these sections to be skipped in an introduction course. Glossaries are provided for commonly used acronyms and mathematical symbols. For those wanting additional informtion, the final appendix contains a list of recommended books which expand on various specialized topics.


язык: en

–убрика: ‘изика/

—татус предметного указател€: √отов указатель с номерами страниц

ed2k: ed2k stats

»здание: 2nd

√од издани€: 1999

 оличество страниц: 725

ƒобавлена в каталог: 23.02.2007

ќперации: ѕоложить на полку | —копировать ссылку дл€ форума | —копировать ID
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ѕредметный указатель
$17\beta$-Hydroxysteroid dehydrogenase      67
$N_rN$-Dimethylamino-m-nitrobenzene (N, N-DMANB)      639Ч642
$\beta$-Carboline      53 637Ч638
$\beta$-Glycosidase      505Ч506 507
$\delta$ function      103
$\Delta^5$-3-Ketosteroid isomerase      472 473
$\mathrm{Ca}^{2+}$-ATPase, anisotropy decays      337
$\mathrm{Ca}^{2+}$-ATPase, boundary lipid quenching      274
$\mathrm{Ca}^{2+}$-ATPase, FD lifetime measurements      167Ч168
$\mathrm{Ca}^{2+}$-ATPase, lifetime distributions      167Ч168
$\tau$      see УLifetimeФ
1, 2, -bis(9, 10-Dibromooleyl)phosphatidyl cholme      274
1,6-Diphenyl-1,3,5-hexatriene      see УDPHФ
1-Adamanatol      203
1-AminonaphUialene      196
1-AN (phenylnaphmylamine)      232Ч233
1-Anilinonaphthalene-8-sulfomc acid (ANS)      see УANSФ
1-Ethyl-4-carbomethoxypyridinium iodide      193
1-Ethylpyrene      246
1-Naphthoic acid      515
1-Phenylnaphthylamine (1-AN)      232Ч233
1-Pyrensulfonyl chloride      307
12-AS      312
2, 5-Diphenyl-1, 3, 4-oxadiazole (PPD)      102 645 646
2,2-BipyridylЧ3, 3-diol $[\mathrm{BP(OH_2)}]$      527
2,5-Diphenyloxazole (DPO)      205
2-Acetylanthracene      194 195
2-Aminonaphthalene      197 198
2-Aminopurine      77 78 650
2-Aminopurine, frequency-domain lifetime measurements      157Ч162
2-Aminopurine, mixture lifetime resolution      126Ч128
2-Aminopurine, time-domain lifetime measurements      124Ч126
2-AN (2-anilinonaphthalene)      194 195 215
2-Anilinonaphthalene (2-AN)      194 195 215
2-Memylanthracene      276
2-Memylharmine      637Ч638
2-Phenylindole      515 526Ч527
200-MHz fluorometers      147Ч149
3-Amino-N-methylphthalimide      231
3-Aminofluoranthrene (AFA)      176 178
3-Aminophthalimide      639 640 641 642
3-Hydroxyflavone      515 527
4-(Dicyanomethylene)-2-methyl-6-(4-dimethylaminostyryI)Ч4H-pyran      106
4-Dimethylamino-4-bromostilbene (DBS)      171Ч172 646Ч648
4-Dimethylamino-4-cyanostilbene (DCS)      646Ч648
4-Dirnethylamino-4-nitrostilbene (DMANS)      639Ч642
5, 6-Bertzoquinoline      238
5-Carboxyfluorescein      599 600
5-Carboxyrhodamine      599 600
5-Dimethylaimno-1-naphthalenesulfonic acid (Dansyl)      see УDansyl groupsФ
5-Doxylstearate (5-NS)      275
5-Hydroxytryptophan (5HW)      477Ч479
6-(p-Tolnidinyl)naphthalene-2-snlfonic acid (TNS)      see УTNSФ
6-Anilinonapnthalene-2-sulfonic acid (ANS)      205
7-(Dimethylamino)coumarinЧ4-acetate      224 226
7-Azaindofe      515 527 528
7-azaindole tautomerization      527
7-Azatryptophan      477Ч479
7-Hydroxycoumarin (umbelliferone)      3 79
8-Hydroxypyrene-1,3,6,8-trisulfonate      see УHPTSФ
9, 10-Dimethylanthracene      354Ч355
9-AC (9-anthroxycholine)      202 203
9-Aminoacridinium      314
9-Anthroxycholino (9-AC)      202 203
9-Arylxanthyl cations      238
9-AS      306
9-Cyanoanthracene      174 230Ч231 649
9-Memylanthoate      197
Acetate quenching      451
Acetoxymethyl esters (AM esters)      556 557
Acousto-optic (AO) crystal      105
Acousto-optic (AO) deflector, laser      107
Acousto-optic (AO) modulators      149
Acridine orange      3 77 432Ч433
Acridine, DNA technology      605 608
Acridine, emission spectrum      9
Acridine, excited-state reactions      515 525
Acriflavine      176 178
Acrylamide quenching      238 239 244Ч246 250 251 252
Acrylamide quenching, absorption spectra      249
Acrylamide quenching, bimolecular quenching constants      252
Acrylamide quenching, covalent adduct formation      257
Acrylamide quenching, intensity decays      282 283
Acrylamide quenching, NATA      285
Acrylamide quenching, proteins      463
Acrylamide quenching, proteins, ethenoadenine-DNA-B helicase hexamer      257
Acrylamide quenching, proteins, metalloprotease      464Ч465
Acrylamide quenching, proteins, single-trp      462
Acrylamide quenching, proteins, tryptophan position and      467
Acrylamide quenching, quenching efficiency      241
Acrylamide quenching, transient effects      281Ч282
Acrylodan      67 200
Actin      201Ч202
Adduct formation, intramolecular quenching      257Ч258
Adenine and derivatives      15 239 247 248 257 388
Adenosine monophosphate      15
Adenosine, fluorescent analogs      81
ADMAN      205
Adrenocorticotropin (ACTH)      221Ч222 452 462 496.498
Albumin Blue 574      565
Alcohol dehydrogenase      333 508
Alcohols, dielectric relaxation times      225
Alkaline phosphatase      259
Alkylaminoanthracene derivatives, phosphate sensors      551 552
Allophycocyanin      83 392
Alpha helices      466Ч467
Aluminum phthalocyanine      76
Amines, fluorogenic probes      80
Amines, quenching by      238 541
Amino acids, phosphorescence quenching      259
Aminophthalamide      53 639 642
Aminopyridine      642
Analog-to-digital converter (ADC)      102
Analyte recognition probes      552Ч560 (see also УSpecific elementsФ)
Analyte recognition probes, calcium and magnesium      556Ч559
Analyte recognition probes, cation probe specificity      552Ч553
Analyte recognition probes, glucose      542Ч543 559Ч560
Analyte recognition probes, sodium and potassium      554Ч556
Analyte recognition probes, theory of      553Ч554
Anion indicators      16
Anion sensors      551Ч552 (see also УChloride probesФ)
Anion sensors, chloride      539Ч541
Anion sensors, oxygen      536Ч538 539
Anisotropy      12 291Ч316
Anisotropy decay laws      328Ч331
Anisotropy decay laws, associated decays      331
Anisotropy decay laws, correlation time distributions      330Ч331
Anisotropy decay laws, hindered rotors      329
Anisotropy decay laws, nonspharical fluorophores      328Ч329
Anisotropy decay laws, segmental mobility of biopolyroer-bound fluorophore      329Ч330
Anisotropy decays      15 98Ч99 100 359Ч361 362Ч365
Anisotropy decays in frequency domain      498
Anisotropy decays, energy transfer      375Ч376
Anisotropy decays, melittin      497Ч498
Anisotropy decays, nucleic acids      338Ч341
Anisotropy decays, nucleic acids, DNA oligomer hydrodynamics      339Ч340
Anisotropy decays, nucleic acids, DNA segmental mobility      340Ч341
Anisotropy decays, proteins      496Ч498
Anisotropy decays, proteins, frequency-domain decays of      335Ч337
Anisotropy decays, proteins, frequency-domain decays of apomyoglobin, rigid rotor      334Ч336
Anisotropy decays, proteins, frequency-domain decays of melittin      336
Anisotropy decays, proteins, frequency-domain decays of oxytocin, picosecond rotational diffusion      336Ч337
Anisotropy decays, proteins, time-domain decays of      333Ч335
Anisotropy decays, proteins, time-domain decays of alcohol dehydrogenase      333
Anisotropy decays, proteins, time-domain decays of domain motions of immunoglobulins      334Ч335
Anisotropy decays, proteins, time-domain decays of free probe, effects of      335
Anisotropy decays, proteins, time-domain decays of phospholipase $A_2$      334
Anisotropy decays, ribonuckase $T_1$      493Ч496
Anisotropy decays, rotational motion measurement      98Ч99
Anisotropy decays, time-dependent      321Ч342
Anisotropy decays, time-dependent, analysis      321Ч328
Anisotropy decays, time-dependent, analysis, $r_0$ value      327
Anisotropy decays, time-dependent, analysis, frequency-domain decays      327Ч328
Anisotropy decays, time-dependent, analysis, time-domain decays      321Ч325
Anisotropy decays, time-dependent, laws governing      328Ч331
Anisotropy decays, time-dependent, laws governing, associated decays      331
Anisotropy decays, time-dependent, laws governing, correlation time distributions      330Ч331
Anisotropy decays, time-dependent, laws governing, hindered rotors      329
Anisotropy decays, time-dependent, laws governing, nonspherical fluorophores      328Ч329
Anisotropy decays, time-dependent, laws governing, segmental mobility of biopolymer-bound fluorophore      329Ч330
Anisotropy decays, time-dependent, long-lifetime roetal-ligand complexes      337Ч338
Anisotropy decays, time-dependent, membrane probe, characterization of      341Ч342
Anisotropy decays, time-dependent, membranes, hindered rotational diffusion in      331Ч333
Anisotropy decays, time-dependent, microsecond      337Ч338
Anisotropy decays, time-dependent, phosphorescence      337
Anisotropy for tryptophan      466Ч467
Anisotropy polarization, mulecular information from fluorescence      18Ч19
Anisotropy spectra, excitation      295Ч298 447Ч449
Anisotropy with multiphoton excitation      315Ч316
Anisotropy, advanced concepts      347Ч365
Anisotropy, advanced concepts, associated anisotropy decay      362Ч365
Anisotropy, advanced concepts, associated anisotropy decay, frequency-domain measurements      364Ч365
Anisotropy, advanced concepts, associated anisotropy decay, theory      363
Anisotropy, advanced concepts, associated anisotropy decay, time-domain measurements      364
Anisotropy, advanced concepts, DNA      361Ч362
Anisotropy, advanced concepts, ellipsoids of revolution      349Ч353
Anisotropy, advanced concepts, ellipsoids of revolution, oblate, intuitive description of      351
Anisotropy, advanced concepts, ellipsoids of revolution, rotational correction times      253Ч255
Anisotropy, advanced concepts, ellipsoids of revolution, simplified      349Ч351
Anisotropy, advanced concepts, ellipsoids of revolution, stick versus slip rotational diffusion      353
Anisotropy, advanced concepts, ellipsoids, anisotropy decay      348
Anisotropy, advanced concepts, global anisotropy decay analysis with collisional quenching      359Ч361
Anisotropy, advanced concepts, global anisotropy decay analysis with multiwavelength excitation      358Ч359
Anisotropy, advanced concepts, quenching application to protein anisotropy decays      360Ч361
Anisotropy, advanced concepts, rotational diffusion of ellipsoids, theory      354
Anisotropy, advanced concepts, rotational diffusion of nonspherical molecules      347Ч348
Anisotropy, advanced concepts, rotational diffusion, frequency-domain studies of      355Ч357 358
Anisotropy, advanced concepts, rotational diffusion, time-domain studies of      354Ч355
Anisotropy, definition      291Ч293
Anisotropy, electronic state resolution from polarization spectra      297Ч298
Anisotropy, emission wavelength and      312
Anisotropy, energy transfer and      373Ч374
Anisotropy, excitation anisotropy spectra      295Ч298 447Ч449
Anisotropy, excitation photoselection of fluorophores      294Ч295
Anisotropy, instrumentation      34Ч35
Anisotropy, lifetime-resolved      310Ч311
Anisotropy, measurement      298Ч303
Anisotropy, measurement, comparison of methods      300
Anisotropy, measurement, depolarization, trivial causes      302Ч303
Anisotropy, measurement, factors affecting anisotropy      303
Anisotropy, measurement, L-format or single-channel method      298Ч300
Anisotropy, measurement, magic-angle polarizer conditions      301
Anisotropy, measurement, membrane-bound probes      310
Anisotropy, measurement, polarizer alignment      300Ч301
Anisotropy, measurement, radiationless energy transfer effects      302
Anisotropy, measurement, T-format or two-channel method      299Ч300
Anisotropy, measurement, total intensity      301Ч302
Anisotropy, metal-ligand complexes      575Ч576
Anisotropy, Perrin equation, rotational diffusion effects      303Ч306
Anisotropy, Perrin equation, rotational diffusion effects, examples of Perrin plots      306
Anisotropy, Perrin equation, rotational diffusion effects, rotational motions of proteins      304Ч306
Anisotropy, Perrin plots of proteins      306Ч308
Anisotropy, Perrin plots, segmental motion effects      311
Anisotropy, photoselection      294Ч295
Anisotropy, polarization, molecular information from fluorescence      18Ч19
Anisotropy, principles      12Ч13
Anisotropy, proteins, association reactions      308Ч310
Anisotropy, proteins, folding and      473 474
Anisotropy, proteins, Perrin plots      306Ч308
Anisotropy, proteins, tryptophan position and      467
Anisotropy, rotational motion      98Ч99
Anisotropy, segmented motions      311
Anisotropy, SoleilletТs rule, depolarization factor multiplication      311Ч312
Anisotropy, theory      293Ч295
Anisotropy, transition moments      312Ч314
AnnexinV,      501Ч503
ANS (1-anilinonaphthalene-8-sulfonic acid)      71 202 203 205
ANS (1-anilinonaphthalene-8-sulfonic acid), anisotropy decay      364Ч365
ANS (1-anilinonaphthalene-8-sulfonic acid), excited-state reactions      515
ANS (1-anilinonaphthalene-8-sulfonic acid), protein studies      466
ANS (1-anilinonaphthalene-8-sulfonic acid), red-edge excitation      232
ANS (1-anilinonaphthalene-8-sulfonic acid), solvent effects      191
ANS (6-anilinonaphthalene-2-sulfonic acid)      205
ANS (6-anilinonaphthalene-2-sulfonic acid), Forster distances      388
ANS derivatives, solvent effects      192
Anthoylxanthyl cations, quenchers of      238
anthracene      3 541 649
Anthracene, emission spectrum      9
Anthracene, excited-state reactions      515
Anthracene, membrane probes      268
Anthracene, phase-sensitive detection      625 628Ч629
Anthracene, quenchers of      238
Anthracene, TOES and DAS comparison      230Ч231
AnthraceneЧ9-carboxyIic acid      515
Anthranilic acid      650
Anthranilic acid, frequency-domain lifetime measurements      157Ч162
Anthranilic acid, mixture lifetime resolution      126Ч128
Anthranilic acid, time-domain lifetime measurements      124Ч126
Anthroyl fatty acid      72
Anthroyloxy fatty acids      238 239 388
Anthroyloxy fatty acids, 12-AS      312
Anthroyloxy fatty acids, 9-AS      306
Anthroyloxy fatty acids, accessibility      267Ч269
Anthroyloxy fatty acids, solvent relaxation      227Ч228
Anthroyloxy fatty acids, TOES      219Ч220
APC (allophyococyanin)      388
Apoazurin      453 462 493
Apomyoglobin      255 304 364Ч365
Apomyoglobin, anisotropy decay      334Ч336
Apomyoglobin, time-resolved emission spectra (TOES)      215Ч217
Apoperoxidase      304
Apparent distance distribution      415Ч416
Apparent phase and modulation lifetimes      177Ч178 523
APTRA      559
Arc lamps      26 31
Archaebacterial histone-like protein (HTa)      459Ч461
Argon-hydrogen flashlamps      108 109
Argon-ion lasers      37 104Ч105 601 602
Aromatic amino acids      see also УPhenylalanineФ УTryptophanФ УTyrosineФ
Aromatic amino acids, intensity decays      488
Aromatic amino acids, quantum yield standards      53
Aromatic amino acids, spectral properties      445Ч452
Asparaginase      462
Aspartate transcarbamylase, noninteracting tryptophan      504Ч505
Associated anisotropy decays      362Ч365
Association reactions      465Ч469
Association reactions, anisotropy      308Ч310 449
Association reactions, anisotropy, melittin association detected from homotransfer      309Ч310
Association reactions, anisotropy, peptide-calmodulin binding      308Ч309
Association reactions, anisotropy, Trp repressor-DNA binding      309
Association reactions, calmodulin, tryptophan mutants, calcium binding site resolution      468Ч469
Association reactions, orientation of bound peptide      377Ч378
Association reactions, Prodan effects on emission spectra      202
Association reactions, quenching      255Ч257
Association reactions, quenching, specific binding reactions      255Ч256
Association reactions, quenching, substrate binding to ribozymes      256Ч257
Asymptotic standard errors (ASEs)      122Ч124
Azacrown ethers      559
Azatetrahydrochrysene      81 82
Azide      285
Azure B      76
Azurin      461 462 493 509
Azurin, emission spectra of molecules with one or two tryptophan      455Ч456
Azurin, site-directed mutagenesis of single-trypto-phan      454Ч455
BABAPH      229Ч230
BABP      229Ч230
Bamase      470Ч472
Bandpass filters      36
BAPTA      78 590
BAPTA-based probes      556Ч559
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