Lakowicz J.R. — Principles of Fluorescence Spectroscopy :: Электронная библиотека попечительского совета мехмата МГУ

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Lakowicz J.R. — Principles of Fluorescence Spectroscopy

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Название: Principles of Fluorescence Spectroscopy

Автор: Lakowicz J.R.

Аннотация:

In the second edition of Principles I have attempted to maintain the emphasis on basics, while updating the examples to include more recent results from the literature. There is a new chapter providing an overview of extrinisic fluorophores. The discussion of timeresolved measurements has been expanded to two chapters. Quenching has also been expanded in two chapters. Energy transfer and anisotropy have each been expanded to three chapters. There is also a new chapter on fluorescence sensing. To enhance the usefulness of this book as a textbook, most chapters are followed by a set of problems. Sections which describe advanced topics are indicated as such, to allow these sections to be skipped in an introduction course. Glossaries are provided for commonly used acronyms and mathematical symbols. For those wanting additional informtion, the final appendix contains a list of recommended books which expand on various specialized topics.

Язык:

Рубрика: Физика/

Статус предметного указателя: Готов указатель с номерами страниц

ed2k: ed2k stats

Издание: 2nd

Год издания: 1999

Количество страниц: 725

Добавлена в каталог: 23.02.2007

Операции: Положить на полку | Скопировать ссылку для форума | Скопировать ID

Предметный указатель

Indole, solvent effects      449—450
Indole, spectral relaxation      221—222
Indole, TD tifetime, multiexponential decays      121—122
Infrared and NIR fluorescence, dyes      74—75
Infrared and NIR fluorescence, light sources      30
Infrared and NIR fluorescence, literature references      657
Infrared filters      38
Inner filter effects      52—54
Instrument response function      409
Instrumentation      25—59
Instrumentation, corrected emission spectra      51—52
Instrumentation, corrected emission spectra, comparison with known emission spectra      51 637
Instrumentation, corrected emission spectra, conversion between wavelength and wavenumber      52
Instrumentation, corrected emission spectra, correction factors obtained with standard lamp      51—52
Instrumentation, corrected emission spectra, quantum counter and scatterer use      52
Instrumentation, corrected excitation spectra      50—51
Instrumentation, excitation and emission spectra      25—28
Instrumentation, excitation and emission spectra, distortions in      28
Instrumentation, excitation and emission spectra, ideal spectrofluorometer      27—28
Instrumentation, frequency-domain lifetime measurements      147—152 173 174
Instrumentation, frequency-domain lifetime measurements, 200-MHz fluorometers      147—149
Instrumentation, frequency-domain lifetime measurements, cross-correlation detection      150
Instrumentation, frequency-domain lifetime measurements, frequency synthesizers      150
Instrumentation, frequency-domain lifetime measurements, history      147
Instrumentation, frequency-domain lifetime measurements, laser diode excitation      173 174
Instrumentation, frequency-domain lifetime measurements, LED excitation      173—175
Instrumentation, frequency-domain lifetime measurements, light modulators      149—150
Instrumentation, frequency-domain lifetime measurements, photomultiplier tubes      150—151
Instrumentation, frequency-domain lifetime measurements, principles of measurement      151—152
Instrumentation, frequency-domain lifetime measurements, radio-frequency amplifiers      150
Instrumentation, frequency-domain lifetime measurements, simple FD instruments      173—175
Instrumentation, light absorption and deviation from Beer — Lambert law      56—57
Instrumentation, light sources      28—32
Instrumentation, light sources, arc and incandescent lamps      28—30
Instrumentation, light sources, solid-state      31—32
Instrumentation, monochromators      32—36
Instrumentation, monochromators, calibration of      35—36
Instrumentation, monochromators, polarization characteristics of      33—34
Instrumentation, monochromators, second-order transmission in      35
Instrumentation, monochromators, wavelength resolution and emission spectra      33
Instrumentation, optical filters      36—39
Instrumentation, optical filters and signal purity      39—41
Instrumentation, optical filters, bandpass      36
Instrumentation, optical filters, combination      38—39
Instrumentation, optical filters, interference      36—38
Instrumentation, optical filters, neutral density      39
Instrumentation, photomoltipher tubes      41—48
Instrumentation, photomoltipher tubes, CCD detectors      47—48
Instrumentation, photomoltipher tubes, failure of, symptoms      47
Instrumentation, photomoltipher tubes, hybrid      47 48
Instrumentation, photomoltipher tubes, photon counting versus analog detection of fluorescence      46 48
Instrumentation, photomoltipher tubes, PMT designs and dynode chains      43—44
Instrumentation, photomoltipher tubes, spectral response      42—43
Instrumentation, photomoltipher tubes, time response of      44—46
Instrumentation, polarizers      48—49
Instrumentation, quantum counters      50—51
Instrumentation, quantum yield standards      52—53
Instrumentation, sample geometry effects      53—55
Instrumentation, sample preparation, common errors in      55—56
Instrumentation, time-correlated single-photon counting      see “Time-correlated single-photon counting”
Instrumentation, two-photon and multiphoton excitation      57—59
Intensity decay      492—493
Intensity decay laws      129—132
Intensity decay laws, lifetime distributions      130—131
Intensity decay laws, multiexponential decay      129—130
Intensity decay laws, stretched exponentials      131
Intensity decay laws, transient effects      131
Intensity decay, least-squares analysis      144—146
Intensity decay, multiexponential model      98
Intensity decay, ribonuclease , structure effects      493
Intensity decay, time-domain lifetime measurements      132—136
Intensity decay, time-domain lifetime measurements, chlorphyll aggregates in hexane      134
Intensity decay, time-domain lifetime measurements, erythrosyn B, picosecond decay time      133—134
Intensity decay, time-domain lifetime measurements, FAD      134—135
Intensity decay, time-domain lifetime measurements, green fluorescent protein, systematic data errors      133
Intensity decay, time-domain lifetime measurements, microsecond luminescence decays      135
Intensity decay, time-domain lifetime measurements, single-tryptophan protein      132—133
Intensity decay, time-domain lifetime measurements, subpicosecond intensity decays      135—136
Intensity decay, tryptophan, decay-associated emiasion spectra      490—491
Intensity decay, tryptophan, neutral tryptophan derivatives      491—492
Intensity decay, tryptophan, rotamer model      488—489
Interference filters      36—38
Internal charge transfer OCT)      186—187 199 200—201 205 515
Intramolecular quenching      257—258
Intrinsic/natural fluorescence      15 63—66
Intrinsic/natural fluorescence of proteins      480—481
Intrinsic/natural fluorescence, enzyme cofactors      63—66
Iodide      17
Iodide, absorption spectra      249
Iodide, quenching by      18 240 241 256
Iodide, quenching by, chloride probe      540
Iodide, quenching by, proteins      463
Ion indicators/probes/sensors      16 78 379—380 543 565—566
Ion indicators/probes/sensors, analyte recognition probes      552—559
Ion indicators/probes/sensors, analyte recognition probes, calcium and magnesium      556—559
Ion indicators/probes/sensors, analyte recognition probes, cation probe specificity      552—553
Ion indicators/probes/sensors, analyte recognition probes, sodium and potassium      554—556
Ion indicators/probes/sensors, chloride      539—541
Ion indicators/probes/sensors, energy-transfer mechanisms      543—544 545
Ion indicators/probes/sensors, oxygen      536—538 539
Ion indicators/probes/sensors, photoindnced electron-transfer (PET) probes      551—552
Ionic quenchers      251 (see also “Specific ions”)
Ionization, naphthol      516—518
Ionization, tyrosine      450—451
IR-125      74 75 76
IR-144      173 174
Isomers, rotational      239—230 445 488—489
Isoxanthopterin      77 78
Jabionski diagram, solvent effects      198
Jablonski diagram      4—6
Jablonski diagram, collisional queaching and resonance energy transfer      11
Jablonski diagram, lanthanide probe      86
Jablonski diagram, red-edge excitation      231
Jablonski diagram, solvent relaxation      185 211
Jablonski, Alexander      4—5
JOE      599 600
Kasha’s rule      187 447
KFU 111      544 545
Kosower Z-scale      193
L-format anisotropy measurement or single-channel method      298—299 300
La Jolla Blue      611
Labeling, incomplete, energy transfer studies      418—419
Lactate dehydrogenase      475—476 477 493
Lactoglobulin      304
Lands, membrane      see “Membranes”
Lands, metal-ligand complex probes      581 582
Lands, pyrene-labeled      80 271 272
Lands, pyrene-labeled, pyrenyl-DPPE      278
Lands, pyrene-labeled, pyrenyl-PC      72—73
Lanthanide MLCs      86 87
Lanthanide MLCs, long-lifetime probes      86 87
Lanthanide MLCs, quenching      440
Lanthanide MLCs, retinal energy transfer study      435
Laser diodes      21
Laser diodes, DNA technology      601 602
Laser diodes, frequency-domain lifetime measurements      173 174
Laser diodes, human serum albumen measurement with      75
Laser diodes, instrumentation      31 32
Lasers, DNA technology      601 602
Lasers, frequency-domain lifetime measurements      155
Lasers, literature references      657
Lasers, monochromators      37
Lasers, multiphoton excitation      480
Lasers, solvent effects on emission spectra, Prodan      200—201
Lasers, time-correlated single-photon counting, picosecond dye devices      104—106
Lasers, time-correlated single-photon counting, solid-state devices      109
Lateral diffusion in membranes      278
Laurdan      200 201
Lead      239 590
Least-squares analysis      97 98 159 419

Least-squares analysis, frequency-domain lifetime measurements      159
Least-squares analysis, intensity decays      144—146
Least-squares analysis, literature references      655
Least-squares analysis, Stern-Volmer plots      255
Least-squares analysis, time-domain lifetime measurements      119—120
Least-squares analysis, TRES      230
LED      see “Light-emitting diodes”
Lethicin vesicles      232—233
Lifetime (decay time) ( $\tau$ )      5—6
Lifetime (decay time) ( $\tau$ ), collisional queaching and      222—224
Lifetime (decay time) ( $\tau$ ), examples of      97—98
Lifetime (decay time) ( $\tau$ ), frequency-domain      see “Frequency-domain lifetime measurements”
Lifetime (decay time) ( $\tau$ ), literature references      653
Lifetime (decay time) ( $\tau$ ), meaning of      96—97
Lifetime (decay time) ( $\tau$ ), phase-modulation      523
Lifetime (decay time) ( $\tau$ ), phase-modulation, apparent lifetimes      177—178
Lifetime (decay time) ( $\tau$ ), phase-modulation, Lifetime (decay time) ( $\tau$ ), principles      10—12
Lifetime (decay time) ( $\tau$ ), phase-modulation, phase angle and modulation      178—180
Lifetime (decay time) ( $\tau$ ), phase-modulation, quenching      11
Lifetime (decay time) ( $\tau$ ), phase-modulation, time scale of molecular processes in solution      11—12
Lifetime (decay time) ( $\tau$ ), protein folding and      474
Lifetime (decay time) ( $\tau$ ), quantum yield and      452
Lifetime (decay time) ( $\tau$ ), steady state intensity and      14
Lifetime (decay time) ( $\tau$ ), time-domain      see “Time-domain lifetime measurements”
Lifetime distributions, time-domain lifetime measurements      130—131
Lifetime imaging      629
Lifetime immunoassays based on RET      586—587
Lifetime reference      115
Lifetime standards      645—649
Lifetime standards, frequency-domain intensity decays, representative      646 650
Lifetime standards, nanosecond      645
Lifetime standards, picosecond      646—649
Lifetime standards, time-domain      647 649
Lifetime, natural      10 453
Lifetime-based probes and sensing      534—535
Lifetime-based probes and sensing, DNA sequencing      604
Lifetime-based probes and sensing, metal-ligand complexes      86—8 (see also “Metal-ligand complexes”)
Lifetime-based probes and sensing, oxygen      537
Lifetime-resolved anisotropy      310—311
Lifetime-resolved emission spectra, relaxation dynamics      222—224
Ligand analogs      81
Ligand binding, proteins      465—466
Light absorption and deviation from Beer — Lambert law      56—57
Light modulators, frequency-domain lifetime measurements      149—150
Light modulators, monochromators      32—36
Light sources      26
Light sources, instrnmentation      28—32
Light sources, time-correlated single-photon counting      103—109
Light sources, time-correlated single-photon counting, femtosecond titanium:sapphire lasers      106—107
Light sources, time-correlated single-photon counting, flaslamps      107—108 109
Light sources, time-correlated single-photon counting, picosecond dye lasers      104—106
Light sources, time-correlated single-photon counting, solid-state lasers      109
light-emitting diodes      31
Light-emitting diodes, FD lifetime measurements      173—175
Light-emitting diodes, instrnmentation      31—32
Light-generated probe arrays, DNA hybridization      612—613
Lipids, membrane, boundary      273—274
Lipids, membrane, distributions around Gramicidin      384—385
Lipids, membrane, energy transfer      425 437
Lipids, membrane, exchange of      386
Lipids, membrane, lipid-water partitioning effects      274—276
Lippert equation      187—194
Lippert equation, application of      191—193
Lippert equation, derivation of      187—191
Lippert equation, polarity scales      193—194
Lippert plots, solvent effects      196—198
Liquid crystals      312—313
Lissamine rhodamine B      70
Literature      653—657
Liver alcohol dehydrogenase      493 508
Localized quenchers, membranes      270—272
Long-lifetime probes      86—88
Long-wavelength standards      639—642
Longitudinal relaxation times      224
Lorentzian distribution, correlation time      330
Lucifer Yellow      69
Lucigenin      539—541
Lumazine      497
Luminescence decays      135
Luminescence, defined      1
Lysozyme      246—247
MACA (N-methylacridinium-9-carboxamide)      539—541
Macromolecular associations, RET for measurement of      378—382
Macromolecular associations, RET for measurement of, calcium indicators      379—380
Macromolecular associations, RET for measurement of, DNA oligomer association kinetics      380—381
Macromolecular associations, RET for measurement of, energy transfer efficiency from enhanced acceptor fluorescence      381—382
Macromolecular associations, RET for measurement of, protein kinase subunit dissociation      378—379
Mag-INDO-1      559
Magic angle conditions      39 41 299—300
Magnesium indicators/probes/sensors      16 543 552 556—559
Magnesium Orange      557 558
Maltose binding protein      567 568
Maltose receptor protein      466
MAMC (N-methyIacridinium-9-methylcar-boxylate)      539—541
Mana      200
Manganese      239
Mastoparan X      462
MDPF [9-methoxy-2,4-diohenyi-3 (2H)-fura-none]      80
Mehttin      462—463 466—467
Mehttin, amsotropy decays      336 496 497—498
Mehttin, association reactions, detection from homotransfer      309—310
Mehttin, association reactions, self-association and calmodulin binding of      465
Mehttin, distance distributions      401—404
Mehttin, frequency-domain lifetime measurements      163 168—169
Mehttin, phosphorescence      509
Mehttin, protein studies      466
Mehttin, RET distance measurements      374—375
Mehttin, rotational correlation time      497
Mehttin, TRES      499
Membrane binding site polarity, solvent effects on emission spectra      203—205
Membrane fusion and lipid exchange      386
Membrane potential probes      73—74
Membrane probes      72—73
Membrane probes, absorption and emission spectra      16
Membrane probes, amsotropy decay, time-dependent      341—342
membrane-bound cholesterol exposure      527—528
Membrane-bound fluorophore, amsotropy      310
Membrane-bound fluorophore, solvent effects on emission spectra      186 203—205
Membranes      34 382—386
Membranes, amsotropy decay, hindered rotational diffusion in      331—333
Membranes, diffusion in      276—278
Membranes, diffusion in, lateral      278
Membranes, diffusion in, quasi-three dimensional      276—278
Membranes, energy transfer      385—386
Membranes, energy transfer, lipid distributions around Gramicidin      384—385
Membranes, energy transfer, membrane fusion and lipid exchange      386
Membranes, energy transfer, relative donor quantum yields      435
Membranes, polymer films      312—314
Membranes, quenching      18 267—279
Membranes, quenching, boundary lipid      273—274
Membranes, quenching, lipid-water partitioning effects      274—276
Membranes, quenching, localized quenchers      270—272
Membranes, quenching, parallax quenching in      272—273
Membranes, quenching, probe accessibility to water—and lipid-soluble quenchers      267—270
Membranes, RET in rapid-diffusion limit, acceptor location in lipid vesicles      437
Membranes, RET in rapid-diffusion limit, retinal location in rhodopsin disc membranes      438—439
Membranes, TNS spectrum      17
Membranes, TRES      217—218 219
Mercury lamps      31
Merocyanine      74 540
Metal km and anion sensors      551—552
Metal-ligand complexes (MLC)      87—88 573—591
Metal-ligand complexes (MLC), amsotropy properties      575—576
Metal-ligand complexes (MLC), biophysical applications      577 581 582
Metal-ligand complexes (MLC), biophysical applications, DNA dynamics      578—580
Metal-ligand complexes (MLC), biophysical applications, lipid probes      581 582
Metal-ligand complexes (MLC), biophysical applications, proteins, domam-to-domain motions in      580—581
Metal-ligand complexes (MLC), clinical chemistry      587—591
Metal-ligand complexes (MLC), energy transfer studies      416
Metal-ligand complexes (MLC), energy-gap law      577 578 579

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