Lakowicz J.R. — Principles of Fluorescence Spectroscopy :: Электронная библиотека попечительского совета мехмата МГУ

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Lakowicz J.R. — Principles of Fluorescence Spectroscopy

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Название: Principles of Fluorescence Spectroscopy

Автор: Lakowicz J.R.

Аннотация:

In the second edition of Principles I have attempted to maintain the emphasis on basics, while updating the examples to include more recent results from the literature. There is a new chapter providing an overview of extrinisic fluorophores. The discussion of timeresolved measurements has been expanded to two chapters. Quenching has also been expanded in two chapters. Energy transfer and anisotropy have each been expanded to three chapters. There is also a new chapter on fluorescence sensing. To enhance the usefulness of this book as a textbook, most chapters are followed by a set of problems. Sections which describe advanced topics are indicated as such, to allow these sections to be skipped in an introduction course. Glossaries are provided for commonly used acronyms and mathematical symbols. For those wanting additional informtion, the final appendix contains a list of recommended books which expand on various specialized topics.

Язык:

Рубрика: Физика/

Статус предметного указателя: Готов указатель с номерами страниц

ed2k: ed2k stats

Издание: 2nd

Год издания: 1999

Количество страниц: 725

Добавлена в каталог: 23.02.2007

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Предметный указатель

Diroethylindole      649
Distance distribution functions      409—410
Distance distributions      395—397 (see also “Energy transfer time-resolved”)
Distance distributions from steady-state data      419—420
Distance distributions in glycopeptide      410—411
Distance distributions in nucleic acids      416—418
Distance distributions in nucleic acids, DNA, double-helical      416—418
Distance distributions in nucleic acids, DNA, four-way Holliday junction in      417—418
Distance distributions in peptides      397—401
Distance distributions in peptides, concentration of D-A pairs and      401
Distance distributions in peptides, cross-fitting data to exclude alternative models      399—400
Distance distributions in peptides, donor decay without RET      400—401
Distance distributions in peptides, rigid versus flexible hexapeptide      397—399
Distance distributions in proteins      401—410
Distance distributions in proteins from time-domain measurements      409
Distance distributions in proteins, analysis with frequency domain data      404—406
Distance distributions in proteins, distance distribution functions      409—410
Distance distributions in proteins, domain motion in      409
Distance distributions in proteins, melittin      401—404
Distance measurements, resonance energy transfer      374—378
Distance measurements, resonance energy transfer, melittin, alpha helical      374—375
Distance measurements, resonance energy transfer, orientation factor effects on possible range of distances      375—376
Distance measurements, resonance energy transfer, orientation of protein-bound peptide      377—378
Distance measurements, resonance energy transfer, protein folding measured by RET      376
Distance-dependent quenching (DDQ) model      280—284
Disulfides, quenching by      238
DM-Nerf      548
DMANCN      200 201
DMANS (4-Dimethylamino-4-nitrostilbene)      639—642
DMPC (dimyristoyl-1- $\alpha$ -phosphatidyl-cholme)      197
DMPC (dimyristoyl-1- $\alpha$ -phosphatidyl-cholme), cholesterol membranes      223—224
DMPC (dimyristoyl-1- $\alpha$ -phosphatidyl-cholme), hilayers      275
DMPC (dimyristoyl-1- $\alpha$ -phosphatidyl-cholme), vesicles, DPH anisotropics in      316
DMPC (dimyristoyl-1- $\alpha$ -phosphatidyl-cholme), vesicles, quasi-three-dimensional diffusion      276—278
DMPP (dimethyldiazaperopyrenium)      432—433
DMS (dimethylamino-4-methoxystilbene)      646—648
DNA base analogs      77—78
DNA hybridization, one donor—und acceptor-labeled probe      380 608—610
DNA hybridization, one donor—und acceptor-labeled probe, energy transfer mechanisms      19
DNA hybridization, one donor—und acceptor-labeled probe, Forster distances      388
DNA hybridization, one donor—und acceptor-labeled probe, linked, diffusion effects      411—416
DNA hybridization, one donor—und acceptor-labeled probe, linked, diffusion effects, apparent distance distribution      415—416
DNA hybridization, one donor—und acceptor-labeled probe, linked, diffusion effects, experimental measurement      412—413
DNA hybridization, one donor—und acceptor-labeled probe, linked, diffusion effects, goodness of fit surfaces und parameter resolution      413—415
DNA hybridization, one donor—und acceptor-labeled probe, linked, diffusion effects, RET and diffusive motions in biopolymers      416
DNA hybridization, one donor—und acceptor-labeled probe, linked, diffusion effects, simulations of RET for flexible pair      411—412
DNA oligomer association kinetics      380—381
DNA probes      76—78 87 88
DNA sequencing      595—604
DNA stains      3
DNA technology      595—614
DNA technology, hybridization      607—612
DNA technology, hybridization, excimer formation      610
DNA technology, hybridization, imagers      612
DNA technology, hybridization, in situ      613—614
DNA technology, hybridization, light-generated probe arrays      612—613
DNA technology, hybridization, one donor—and acceptor-labeled probe      608—610
DNA technology, hybridization, PCR      612
DNA technology, hybridization, polarization assays      610—612
DNA technology, hybridization, RET applications      416—418 420—421
DNA technology, sequencing      595—604
DNA technology, sequencing, energy-transfer dyes      598—601
DNA technology, sequencing, lifetime-based      604
DNA technology, sequencing, NIR probes      601—604
DNA technology, sequencing, nucleotide labeling methods      598
DNA technology, stains, high-sensitivity      604—607
DNA technology, stains, high-sensitivity, energy-transfer stains      606—607
DNA technology, stains, high-sensitivity, fragment sizing by flow cytometry      607
DNA technology, stains, high-sensitivity, his DNA stains      605—606
DNA, anisotropy decay, time-dependent      361—362
DNA, anisotropy decay, time-dependent, oligomer hydrodynamics      339—340
DNA, anisotropy decay, time-dependent, segmental mobility      340 341
DNA, distance distributions, double-hetical      416—417
DNA, distance distributions, double-hetical, four-way Holliday junction in      417~418
DNA, energy transfer      425
DNA, energy transfer, energy transfer in one dimension      432—433
DNA, energy transfer, FD lifetime measurements      164—165
DNA, energy transfer, metal-ligand complex dynamics      578—580
DNA, energy transfer, Trp repressor binding anisotropy      309
DNA-B helicase      257
DNA-bound probe accessibility, quenching      246—247
DNBS (dimtrobenzenesulfenyl)      388
Domain motion      334—335 409
Donor decay without RET in peptides      400—401
Donor-acceptor pairs      see also “Energy transfer”
Donor-acceptor pairs and distance distribution in peptides      401
Donor-acceptor pairs, acceptor decays      418
Donor-acceptor pairs, distance distribetions from steady-state data with different fto values      419—420
Donor-acceptor pairs, distance distribetions from steady-state data, quenching, changing fto by      420
DOPC (dioleolyl-1- $\alpha$ -phosphatidylcholine)      201
DOPC (dioleolyl-1- $\alpha$ -phosphatidylcholine), quenching      269—270
DOPC (dioleolyl-1- $\alpha$ -phosphatidylcholine), red-edge excitation      232
DOS (trans-4-dimethylamino-4-(1-oxybutyl)-stilbene      186
DOTCI      73
DPA (diphenylanthracene)      52 155
DPH(1, 6-diphenyl-1,3, 5-hexatriene)      15 72
DPH(1, 6-diphenyl-1,3, 5-hexatriene) in film      313
DPH(1, 6-diphenyl-1,3, 5-hexatriene), anisotropic rotational diffusion      356—357
DPH(1, 6-diphenyl-1,3, 5-hexatriene), anisotropy decays, time-dependent      329 331 332
DPH(1, 6-diphenyl-1,3, 5-hexatriene), Forster distances      388
DPH(1, 6-diphenyl-1,3, 5-hexatriene), membrane probes      268
DPH(1, 6-diphenyl-1,3, 5-hexatriene), quenching-resolved spectra      253 254
DPO (2,5-diphenyloxazole)      205
DPPC (dipalmitolyl+ $\alpha$ -phosphatidylcholine) systems      201 275
DPPC (dipalmitolyl+ $\alpha$ -phosphatidylcholine) systems, energy transfer in two dimensions      430—432
DPPC (dipalmitolyl+ $\alpha$ -phosphatidylcholine) systems, Patman-laibled, TRES      217—218 219
DPPG vesicles      581 582
DPS (4-dimethylamino-4-fluorostilbene)      172 646—648
DTAC micelles      246
Dye lasers      104—107 480
Dyes      2 3
Dyes, energy-transfer      598—601 606—607
Dyes, red and near IR      74—75
Dyes, solvent relaxation      229
Dynamic quenching      see “Collisional quenching” “Quenching”
Dynamics of relaxation      see “Relaxation dynamics”
Dynode chains      43—44
Dynorphin      497
E1A [(5-(iodoacetamido)eosin]      388
Efficiency, quenching      241
Electro-optic modulator      149—150
Electron scavengers, quenching by      239
Electron transfer, photoinduced (PET)      536 551—552
Electronic state resolution from polarization spectra      297—298
Electronic states      5—6
Electronics, frequency-domain fluorometers      147 149—150
Electronics, photomultiplier tube      41
Electronics, time-correlated single-photon counting      109—111
Electrophoresis      109
ELISA      3 560
Elongation factor (Ta-GDP)      462
Emission spectra      3—4 5—6
Emission spectra, corrected      637—649
Emission spectra, corrected, $\beta$ -carbolene derivatives as fluorescence standards      637—638
Emission spectra, corrected, 9, 10-diphenyianthracene, quinine sulfate and fluorescein      637 638
Emission spectra, corrected, additional spectra      642 643
Emission spectra, corrected, instrumentation      51—52
Emission spectra, corrected, instrumentation, comparison with known emission spectra      51
Emission spectra, corrected, instrumentation, conversion between wavelength and wavenumber      52
Emission spectra, corrected, instrumentation, correction factors obtained with standard lamp      51—52
Emission spectra, corrected, instrumentation, quantum counter and scatterer use      52
Emission spectra, corrected, long-wavelength standards      639 640 641 642
Emission spectra, corrected, ultraviolet standards      639 642
Emission spectra, decay-associated      490—491 499—501
Emission spectra, instrumentation      25—28
Emission spectra, instrumentation, distortions in      28
Emission spectra, instrumentation, ideal spectrofluorometer      28—32
Emission spectra, molecular information from fluorescence      17
Emission spectra, quenching-resolved      252—255
Emission spectra, quenching-resolved, fluorophore mixtures      252—253 254

Emission spectra, quenching-resolved, Tet repressor      253—255
Emission spectra, representative, literature references      653—654
Emission spectra, through optical filters      40—41
Emission spectra, tryptophan decay-associated      490—491
Emission spectra, two-component mixture      621—623
Emission wavelength, and anisotropy      312
Emylenediamine, dansylated      206
Endonucleases      249—250 381
Energy transfer efficiency from enhanced accepter fluorescence      381—382
Energy transfer in membranes      382—386
Energy transfer in membranes, distance of closest approach in      385—386
Energy transfer in membranes, lipid distributions around Gramicidin      384—385
Energy transfer in membranes, membrane fusion and lipid exchange      386
Energy transfer in solution      386—388
Energy transfer to multiple acceptors in one, two, or three dimensions      425—440
Energy transfer to multiple acceptors in one, two, or three dimensions, diffusion effects on RET with unlinked donors and acceptors      428—429
Energy transfer to multiple acceptors in one, two, or three dimensions, dimensionality effect on RET      429—434
Energy transfer to multiple acceptors in one, two, or three dimensions, dimensionality effect on RET, one dimension      432—433
Energy transfer to multiple acceptors in one, two, or three dimensions, dimensionality effect on RET, two dimensions      430—432
Energy transfer to multiple acceptors in one, two, or three dimensions, restricted geometries      434—435
Energy transfer to multiple acceptors in one, two, or three dimensions, RET in rapid-diffusion limit      435—440
Energy transfer to multiple acceptors in one, two, or three dimensions, RET in rapid-diffusion limit, acceptor location in lipid vesicles      437
Energy transfer to multiple acceptors in one, two, or three dimensions, RET in rapid-diffusion limit, Forster transfer versos exchange interactions      439—440
Energy transfer to multiple acceptors in one, two, or three dimensions, RET in rapid-diffusion limit, retinal location in rhodopsin disk membranes      438—439
Energy transfer to multiple acceptors in one, two, or three dimensions, three dimensions      425—429
Energy transfer, acceptor emission      381
Energy transfer, data analysis      405
Energy transfer, diffusion-enhanced      387
Energy transfer, distance measurements using RET      13 374—378
Energy transfer, distance measurements using RET, melittin, a helical      374—375
Energy transfer, distance measurements using RET, orientation factor effects on possible range of distances      375—376
Energy transfer, distance measurements using RET, orientation of protein-buund peptide      377—378
Energy transfer, distance measurements using RET, protein folding measured by RET      376
Energy transfer, DNA hybridization, one donor—and acceptor-labeled probe      380 608—610
Energy transfer, excited-state reactions      515
Energy transfer, Forster distances      388
Energy transfer, homotransfer      373
Energy transfer, macromolecular associations, RET for measurement of      378—382
Energy transfer, macromolecular associations, RET for measurement ofcalcium indicators      379—380
Energy transfer, macromolecular associations, RET for measurement ofDNA oligomer association kinetics      380—381
Energy transfer, macromolecular associations, RET for measurement ofenergy transfer efficiency from enhanced acceptor fluorescence      381—382
Energy transfer, macromolecular associations, RET for measurement ofprotein kinase subonit dissociation      378—379
Energy transfer, orientation factor      371
Energy transfer, proteins      374—377 456—461
Energy transfer, proteins, anisotropy decreases, detection of ET by      459
Energy transfer, proteins, interferon- $\gamma$ , tyrosine-to-tryptophan energy transfer in      456—457
Energy transfer, proteins, phenylalanine-to-tyrosme      459—461
Energy transfer, proteins, RET efficiency quantitation      457—458
Energy transfer, radrationless, anisotropy measurement      302
Energy transfer, representative values      388
Energy transfer, sensor mechanisms      541—545
Energy transfer, sensor mechanisms, glucose      542—543
Energy transfer, sensor mechanisms, ion      543—544 545
Energy transfer, sensor mechanisms, pH and       541—542
Energy transfer, sensor mechanisms, theory for      545
Energy transfer, theory of      368—374
Energy transfer, theory of transfer rate dependence on distance, overlap integral, and orientation factor      373
Energy transfer, theory ofhomotransfer und heterotransfer      373—374
Energy transfer, theory oforientation factor $(\kappa^2)$       371 —373
Energy transfer, time-resolved      395—421
Energy transfer, time-resolved, acceptor decays      418
Energy transfer, time-resolved, applications of RET      420—421
Energy transfer, time-resolved, diffusion effects for linked D-A pairs      411—416
Energy transfer, time-resolved, diffusion effects for linked D-A pairs, apparent distance distribution      415—416
Energy transfer, time-resolved, diffusion effects for linked D-A pairs, experimental measurement      412—413
Energy transfer, time-resolved, diffusion effects for linked D-A pairs, goodness of fit surfaces and parameter resolution      413—415
Energy transfer, time-resolved, diffusion effects for linked D-A pairs, RET and diffusive motions in biopolymers      416
Energy transfer, time-resolved, diffusion effects for linked D-A pairs, simulations of RET for flexible pair      411—412
Energy transfer, time-resolved, distance distribotions in glycopeptide      410—411
Energy transfer, time-resolved, distance distributions      395—397
Energy transfer, time-resolved, distance distributions from steady-state data      419—420
Energy transfer, time-resolved, distance distributions from steady-state data, D-A pairs with different values      419—420
Energy transfer, time-resolved, distance distributions from steady-state data, quenching, changing Ro by      420
Energy transfer, time-resolved, distance distributions in nucleic acids      416—418
Energy transfer, time-resolved, distance distributions in nucleic acids, DNA, double-helical      416—417
Energy transfer, time-resolved, distance distributions in nucleic acids, DNA, four-way Holliday junction in      417—418
Energy transfer, time-resolved, distance distributions in peptides      397—401
Energy transfer, time-resolved, distance distributions in peptides, concentration of D-A pairs and      401
Energy transfer, time-resolved, distance distributions in peptides, cross-fitting data to exclude alternative models      399—400
Energy transfer, time-resolved, distance distributions in peptides, donor decay without RET      400—401
Energy transfer, time-resolved, distance distributions in peptides, rigid versus flexible hexapeptide      397—399
Energy transfer, time-resolved, distance distributions in proteins      401—410
Energy transfer, time-resolved, distance distributions in proteins, analysis with frequency domain data      404—406
Energy transfer, time-resolved, distance distributions in proteins, distance distribution functions      409—410
Energy transfer, time-resolved, distance distributions in proteins, domain motion in      409
Energy transfer, time-resolved, distance distributions in proteins, from tune-domain measurements      409
Energy transfer, time-resolved, distance distributions in proteins, melittin      401—404
Energy transfer, time-resolved, DNA hybridization      420—421
Energy transfer, time-resolved, incomplete labeling effects      418—419
Energy transfer, time-resolved, orientation factor effects      419
Energy transfer, time-resolved, terbium      420—421 438
Energy-gap law      577—579 588
Energy-transfer immunoassays      562—563
Energy-transfer stains and dyes, DNA      598—601 606—607
Enzyme cofactors      15
Enzyme cofactors, intrinsic fluorescence      63—65
Enzyme cofactors, time-domain lifetime measurement      134—135
Eosin      20
Eosin-labeled ethanolamine      372
ERITC (erytbrosyn—5-isothincyanate)      337
ErvthrosinB      20 133—134
Escherichia coli maltose receptor protein      466
Escherichia coli ribose binding protein      474—475
Escherichia coti lac repressor      514
Ethanetiuol      285
Ethanol, indole emission spectra in cyclohexane-ethanol mixtures      449—450
Ethanol, indole lifetime in      452
Ethanol, polarizability properties      188
Ethanol, rotational diffusion in      304
Ethanol, tryptophan anisotropy spectra in      449
Ethenoadernine and derivatives      15 239 347 248 256 388
Ethers      188 238
Ethidium bromide      76 77 246
Ethidium bromide, anisotropy decay      361—362
Ethidium bromide, DNA technology      605
Ethidium bromide, energy transfer in one dimension      432 433
Ethidium homodimer      77 605
Ethoxyocoumarin      205
Ethyl acetate      189
Etnanolamine      372
Europium, energy transfer studies      416
Europium, immunoassays      561
Europium, qneaching by      239
Europium, Raster distances      388
Europrum probes      87
Exchners      9 515
Exchners, DNA hybridization      610
Exchners, phenylalanine      452
Exchners, quasi-three-diniensional diffusion in membranes      276—278
Exciplex formation      9 238—239 515
Excitation anisotropy spectra      295—298 447—449
Excitation anisotropy, two-photon and multiphoton      315—316
Excitation pfaotoselection of fluorophores      294—295
Excitation spectra, anisotropy      295—298 447—449
Excitation spectra, instrumentation      25—28
Excitation spectra, instrumentation, corrected spectra      50—51
Excitation spectra, instrumentation, distortions in      28
Excitation spectra, instrumentation, ideal spectrofluorometer      27—28
Excitation spectra, polarization, tyrosine and tryptophan      447—449
Excitation wavelength independence of fluorescence      7—8
Excited charge-transfer complex (exciplex)      238—239 515
Excited-state distribution, one, two, and three photon excitation      315
Excited-state reactions      515—528
Excited-state reactions, analysis by phase-modulation fluorometry      521—526
Excited-state reactions, analysis by phase-modulation fluorometry, apparent phase and modulation lifetimes, effects on      523
Excited-state reactions, analysis by phase-modulation fluorometry, wavelength-dependent phase and modulation values      524—525
Excited-state reactions, biochemical examples      527—528
Excited-state reactions, biochemical examples, 7-azaindole tautomerization      527
Excited-state reactions, biochemical examples, membrane-bound cholesterol exposure      527—528
Excited-state reactions, differential-wavelength methods      520—521

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