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Lakowicz J.R. — Principles of Fluorescence Spectroscopy
Lakowicz J.R. — Principles of Fluorescence Spectroscopy



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Íàçâàíèå: Principles of Fluorescence Spectroscopy

Àâòîð: Lakowicz J.R.

Àííîòàöèÿ:

In the second edition of Principles I have attempted to maintain the emphasis on basics, while updating the examples to include more recent results from the literature. There is a new chapter providing an overview of extrinisic fluorophores. The discussion of timeresolved measurements has been expanded to two chapters. Quenching has also been expanded in two chapters. Energy transfer and anisotropy have each been expanded to three chapters. There is also a new chapter on fluorescence sensing. To enhance the usefulness of this book as a textbook, most chapters are followed by a set of problems. Sections which describe advanced topics are indicated as such, to allow these sections to be skipped in an introduction course. Glossaries are provided for commonly used acronyms and mathematical symbols. For those wanting additional informtion, the final appendix contains a list of recommended books which expand on various specialized topics.


ßçûê: en

Ðóáðèêà: Ôèçèêà/

Ñòàòóñ ïðåäìåòíîãî óêàçàòåëÿ: Ãîòîâ óêàçàòåëü ñ íîìåðàìè ñòðàíèö

ed2k: ed2k stats

Èçäàíèå: 2nd

Ãîä èçäàíèÿ: 1999

Êîëè÷åñòâî ñòðàíèö: 725

Äîáàâëåíà â êàòàëîã: 23.02.2007

Îïåðàöèè: Ïîëîæèòü íà ïîëêó | Ñêîïèðîâàòü ññûëêó äëÿ ôîðóìà | Ñêîïèðîâàòü ID
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Ïðåäìåòíûé óêàçàòåëü
Excited-state reactions, examples      515—519
Excited-state reactions, frequency-domain measurement of excimer formation      526—527
Excited-state reactions, naphthol dissociation, time-domain studies of      519—521
Excited-state reactions, naphthol ionization      516—518
Excited-state reactions, phase modulation      521
Excited-state reactions, reversible two-state model      518—519
Excited-state reactions, reversible two-state model, steady-state fluorescence of      518
Excited-state reactions, reversible two-state model, time-resolved decays for      518—519
Exponential decays, frequency-domain life-time measurements      154
Extrinsic fluorophores      15 66
F-statistic      122 123
FAD (flavin adenine dunucleotide)      66
FAD (flavin adenine dunucleotide), quenching by adenine moiety      239
FAD (flavin adenine dunucleotide), time-domain lifetime measurement      134—135
FAM      599 600
Fatty acid binding proteins      202
Fatty acid coenzyme A      81
Fatty acids      see also “Specific fatty acids”
Fatty acids, fluorenyl      267—269
Fatty acids, membrane probes      72
Femtosecond titanium:sapphire lasers      106—107
Ferredoxin      493
Fibers      see “Optical filters”
films      312—313
FISH      613
FITC      see “Fluorescein isothiocyanate”
FKBP59      503—504
Flashiamps, time-correlated single-photon counting      107—108 109
Flexible D-A pairs      411—412
Flexible hexapeptide, distance distributions      397—399
Fluo-3      553
Fluorenyl fatty acids      72 73 267—269 271
Fluorescamine      80
Fluorescein      2 4 591
Fluorescein isothiocyanate (FITC)      15 16 67 69
Fluorescein isothiocyanate (FITC), FITC-dextran      543
Fluorescein isothiocyanate (FITC), Forster distances      388
Fluorescein, anisotropic rotational diffusion      357
Fluorescein, corrected emission spectra      637 638
Fluorescein, DNA energy transfer reactions      381
Fluorescein, DNA technology      596 608 614
Fluorescein, filters      37
Fluorescein, melittin labeled with      309—310
Fluorescein, methionyl-tRNA synthetase labeled with      306—307
Fluorescein, quantum yield standards      53
Fluorescein, quenching      256—257
Fluorescein-PE      72—73
Fluorescence anisotropy      see “Anisotropy”
fluorescence in situ hybridization      613
Fluorescence lifetime imaging microscopy      629—633
Fluorescence microscopy literature      657
Fluorescence polarization      see “Polarization”
Fluorescence polarization immunoassays      563—565 582—584 585 586
fluorescence polarization of anisotropy      18—19
Fluorescence quenching      see “Quenching”
Fluorescence recovery after photobleaching (FRAP)      275
Fluorescence resonance energy transfer (FRET)      367 (see also “Energy transfer”)
Fluorescence standards      see “Standards”
Fluorogenic probes      79—81
Fluorometry, frequency-domain lifetime measurements      169—173
Fluorophores      3—4 63—89
Fluorophores, anisotropy decay, tune-dependent      328—329
Fluorophores, anisotropy of planar fluorophores with high symmetry      314
Fluorophores, chemical sensing probes      78 79
Fluorophores, DNA probes      76—78
Fluorophores, excitation photoselection of      294—295
Fluorophores, extrinsic      66—74
Fluorophores, extrinsic, membrane potential probes      73—74
Fluorophores, extrinsic, membrane probes      72—73
Fluorophores, extrinsic, protean labeling, role of Stokes’ shirt in      70—71
Fluorophores, extrinsic, protein-labeling probes, noncovalent      71—72
Fluorophores, extrinsic, protein-labeling reagents      67—70
Fluorophores, extrinsic, solvent-sensitive probes      71
Fluorophores, fluorescent proteins      82—86
Fluorophores, fluorescent proteins, green fluorescent protein      85—86 565—566
Fluorophores, fluorescent proteins, phycobiliproteins      82—85 562
Fluorophores, fluorescent proteins, phytofluors      86
Fluorophores, intrinsic or natural      63—66
Fluorophores, intrinsic or natural, enzyme cofactors      63—65
Fluorophores, intrinsic or natural, protein binding of NADH      65—66
Fluorophores, long-lifetime probes      86—88
Fluorophores, long-lifetime probes, lanthanides      86—87
Fluorophores, long-lifetime probes, transition-metal-ligand complexes      87 88
Fluorophores, mixtures, quenching-resolved emission spectra      252—253 254
Fluorophores, proteins as sensors      88—89
Fluorophores, quenchers of      238 239
Fluorophores, red and near-IR dyes      74—75
Fluorophores, special probes      79—82
Fluorophores, special probes, fluorogenic      79—81
Fluorophores, special probes, structural analogs of biomotecules      81 82
Fluorophores, special probes, viscosity probes      82
FluoSpherc      544 545
Formycin      81
Forster cycle      517
Forster distance ($R_0$)      13 367
Forster distance ($R_0$), anisotropy decay, tune-dependent      327
Forster distance ($R_0$), bomotransfer      373
Forster distance ($R_0$), determination from time-resolved data      428
Forster distance ($R_0$), distance distributions from steady-state data      419—420
Forster distance ($R_0$), donor-acceptor pairs      388
Forster distance ($R_0$), indole acceptor pairs      419
Forster distance ($R_0$), proteins      456
Forster distance ($R_0$), representative      388
Forster distance ($R_0$), RET in three dimensions      426
Forster transfer, versus exchange interactions      439—440
Forster, Theodor      372
Fractional accessibility      247—249
Franck — Condon principle      5 12
Franck — Condon state      211
Frequency synthesizers      150
Frequency-domain decays, anisotropy, time-dependent      327—328
Frequency-domain decays, representative      646 650
Frequency-domain lifetime measurements      141—180
Frequency-domain lifetime measurements, $\mathrm{Ca}^{2+}$-ATPase lifetime distributions      167—168
Frequency-domain lifetime measurements, apparent phase and modulation lifetime      177—178
Frequency-domain lifetime measurements, background correction      153
Frequency-domain lifetime measurements, biochemical examples      163—169
Frequency-domain lifetime measurements, biochemical examples, recovery of lifetime distributions from data      166—167
Frequency-domain lifetime measurements, biochemical examples, SPQ, coltisional quenching      165
Frequency-domain lifetime measurements, biochemical examples, staphylococcal nuclease and melittin, miutiexponential decay      163
Frequency-domain lifetime measurements, color effects      152—153
Frequency-domain lifetime measurements, DNA, DAPI-labeled      164—165
Frequency-domain lifetime measurements, gigahertz fluorometry      169—173
Frequency-domain lifetime measurements, gigahertz fluorometry, biochemical examples      172
Frequency-domain lifetime measurements, gigahertz fluorometry, measurements      171—172
Frequency-domain lifetime measurements, green fluorescent protein, one—and two-photon excitation      166
Frequency-domain lifetime measurements, instrumentation      147—152
Frequency-domain lifetime measurements, instrumentation, 200-MHz fluorometers      147—149
Frequency-domain lifetime measurements, instrumentation, cross-correlation detection      150
Frequency-domain lifetime measurements, instrumentation, frequency synthesizers      150
Frequency-domain lifetime measurements, instrumentation, history      147
Frequency-domain lifetime measurements, instrumentation, light modulators      149—150
Frequency-domain lifetime measurements, instrumentation, photomultiplier tubes      150—151
Frequency-domain lifetime measurements, instrumentation, principles of measurement      151—152
Frequency-domain lifetime measurements, instrumentation, radio-frequency amplifiers      150
Frequency-domain lifetime measurements, melittin lifetime distributions, cross fitting of models      168—169
Frequency-domain lifetime measurements, monellin, single tryptophan protein with three decay tunes      163
Frequency-domain lifetime measurements, multiexponential decay analysis      155—163
Frequency-domain lifetime measurements, multiexponential decay analysis, global analysis of two-component mixture      159—160
Frequency-domain lifetime measurements, multiexponential decay analysis, three-component mixture, resolution limits      160—162
Frequency-domain lifetime measurements, multiexponential decay analysis, three-component mixture, resolution with 10-fold range of decay times      162—163
Frequency-domain lifetime measurements, multiexponential decay analysis, two closely spaced lifetimes      157—159
Frequency-domain lifetime measurements, multiexponential decay analysis, two widely spaced lifetimes      155—157
Frequency-domain lifetime measurements, NADH intensity decay      169
Frequency-domain lifetime measurements, perspectives on      180
Frequency-domain lifetime measurements, phase angle and modulation spectra      175 176—177
Frequency-domain lifetime measurements, phase modulation fluorescence equations, derivation of      178—180
Frequency-domain lifetime measurements, phase modulation fluorescence equations, derivation of, cross-correlation detection      180
Frequency-domain lifetime measurements, phase modulation fluorescence equations, derivation of, lifetime relationship to phase angle and modulation      178—180
Frequency-domain lifetime measurements, Quin-2      165
Frequency-domain lifetime measurements, representative decays      154—155
Frequency-domain lifetime measurements, representative decays, exponential      154
Frequency-domain lifetime measurements, scattered light      154—155
Frequency-domain lifetime measurements, simple FD instruments      173—175
Frequency-domain lifetime measurements, simple FD instruments, laser diode excitation      173 174
Frequency-domain lifetime measurements, simple FD instruments, LED excitation      173—175
Frequency-domain lifetime measurements, theory      142—147
Frequency-domain lifetime measurements, theory, estimation of parameter uncertainties      146—147
Frequency-domain lifetime measurements, theory, global analysis of dsta      146
Frequency-domain lifetime measurements, theory, least squares analysis of intensity decays      144—146
Frequency-domain measurements, anisotropy decay      355—357 358 364—365
Frequency-domain measurements, anisotropy decay, excimer fonnation      526—527
Frequency-domain measurements, anisotropy decay, protein distance distributions from      404—406
Frequency-domain measurements, anisotropy decay, time-domain lifetime measurements      95—98
Frequency-domain measurements, anisotropy decay, time-domain lifetime measurements, examples of time-domain and frequency-domain lifetimes      97—98
Frequency-domain measurements, anisotropy decay, time-domain lifetime measurements, lifetime or decay time, meaning of      96—97
Frequency-domain measurements, anisotropy decay, time-domain lifetime measurements, phase modulation lifetimes      97
FRET      see “Energy transfer”
Fumarate      239
Fura—2      78 79 557 558
g-factor      34 300 328
Gastrin      497
Gated detection      116—117
Gauss — Newton algorithms      119
Gaussian distributions, correlation time      330
Genetically engineered proteins for ribonuclease protein folding studies      474
Genetically engineered proteins in sensors      565—566
Genetically engineered proteins, azurins      454—455
Genetically engineered proteins, spectral properties      469—473
Genetically engineered proteins, spectral properties, bamase      470—472
Genetically engineered proteins, spectral properties, human tissue factor      470
Genetically engineered proteins, spectral properties, protein tyrosyl transferase      469 470
Genetically engineered proteins, spectral properties, tyrosine proteins      472—473
Gigahertz fluorometry      169—173
Global analysis, anisotropy decay, with collisional quenching      359—361
Global analysis, anisotropy decay, with multiwavetength excitation      357—359
Global analysis, frequency-domain lifetime measurements      146 159—160
Global analysis, time-domain lifetime measurements      126 132
Glucagon      453 462 493
Glucose sensors, analyte recognition probes      559—560
Glucose sensors, energy-transfer mechanisms      542—543
gly-trp-gly      496
Glyccraldehyde-3-phosphate dehydrogenase      251
Glycerol      225
Glycopeptides      410—411
Gonadotropin      462
Goodness of fit ($\chi^2_R$)      123—124 125
Goodness of fit ($\chi^2_R$), diffusion of D-A pairs, linked      413—415
Goodness of fit ($\chi^2_R$), frequency-domain lifetime measurements      157—161
Goodness of fit ($\chi^2_R$), three-decay time model      155
Goodness of fit ($\chi^2_R$), time-domain lifetime measurements      120—121 122
Gramicidin      384—385
Green fluorescent protein      84 565—566
Green fluorescent protein, fluorophores      85—86
Green fluorescent protein, frequency-domain lifetime measurements      166
Green fluorescent protein, time-domain lifetime measurement      133
GroEL (molecular chaperonin cpm 60)      307
Ground-state complex, quenching mechanisms      239
Ground-state complex, tyrosine      451—452
Guanosine-containing substrate-binding strand (G-SBS)      257
Halides      17
Halogenated anesthetics      541
Halogens      17 238 239
Harmane/Harmaline/Harmine      637—638
Heat filters      38
Helicase      257
Helium-cadmium laser      155 176
Heme proteins, Forster distances      388
Heme proteins, intrinsic fluorescence      506—508
Heme proteins, solvent relaxation      215—216
Hemicyanine dyes      229
Herschel, J.F.W.      2 3
Heterogeneous decays, TD lifetime measurements      98—101
Heterotransfer      373—374
Hexane, polarizability properties      188
Hexane, Stokes’ shift      189
Hexane-pyridine, solvent relaxation      227—228
Hexokinase      580
Hindered rotational diffusion, membranes      331—333
Hindered rotors      329
Histidine quenching      238 239
Histocompatibility protein      497
HITC      5l
Hoechst 33258      247 248
Hoechst 33342      76 77
Hoechst 33358      76
Holliday junction in      417—418
Holographic gratings      35
Homotransfer      373—374
HPLC      175
HPTS (8-hydroxypyrerie-1,3,6-trisulfonate), blood gas measurement      546
HPTS (8-hydroxypyrerie-1,3,6-trisulfonate), excited-state reactions      515
HPTS (8-hydroxypyrerie-1,3,6-trisulfonate), pH sensors      548—549
HTa (htstone-like protein)      459—461
HTV protease      81
Human imrnunodeficiency virus protease      80—81 381
Human inimunodeficiency virus integrase      78
Human luteinizing hormone      462
Human serum albumin      13 72 255 256 462
Human serum albumin, anisotropy decay      362—363
Human serum albumin, intensity decay of      493
Human serum albumin, laser diode excitation, measurement with      75
Human serum albumin, tryptophan quenching by nearby amino acid residues      503—504
Human tissue factor      470
Hybrid photomultipljer tubes      47 48
Hydrogen bonding, tyrosine      452
Hydrogen peroxide      238 239
Hydrogen pulse lamps      107—108
Hydrophobic surface, calmodulin      202—203
IAEDANS, calmodulin trp energy transfer      378
IAEDANS, distance distributions      406—409
IAEDANS, Forster distances      388
IAEDANS, immunoglobulin fragment labeled with      307—308
IAEDANS, quenching-resolved spectra      253 254
IAF [5-(lodoacetaimdo)fluorescein]      67
IAF [5-(lodoacetaimdo)fluorescein], domain motion      409
IAF [5-(lodoacetaimdo)fluorescein], Forster distances      388
IAF-CK      564
IANBD      566
ICT (internal charge transfer)      186—187 199 200—201 205 515
Imagers, DNA hybridization      612
Imidazole and imidazoliuin, quenching by      238 239
Immunoassays      21 560—565
Immunoassays, ELISA      560
Immunoassays, energy-transfer      562—563
Immunoassays, fluorescence polarization      563—565
Immunoassays, literature references      656
Immunoassays, metal-ligand complexes      582—587
Immunoassays, metal-ligand complexes, lifetime assays based on RET      586—587
Immunoassays, metal-ligand complexes, long-wavelength      585—586
Immunoassays, time-resolved      560—562
Immunoglobulin, anisotropy decay      334—335
Immunoglobulin, domain-to-domain motions in      580
Immunoglobulin, fragment, Perrin plots      307—308
Immunoglobulin, rotational correlation time      304
Immunophilin FKBP59      503—504
In situ DNA hybridization      613—614
Incomplete labeling effects, energy transfer studies      418—419
Indicators, principles      16 17
Indo-I      556 557 558 559
Indole      650
Indole, corrected emission spectra      643
Indole, covalent adduct formation      257
Indole, decay times      427
Indole, Forster distances, acceptor pairs      419
Indole, frequency-domain lifetime measurements      156 160—162
Indole, intensity decays      488
Indole, mixture lifetime resolution      126—128
Indole, quenching      238 239 453
Indole, rotational correlation times      497
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