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Авторизация |
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Wong S.S. — Chemistry of Protein Conjugation and Cross-Linking |
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Предметный указатель |
Avidin-biotin complex (ABC) 128—131 247 259 286
Avidin-ferritin conjugates 203
Azido derivatives 183 see
Azidocarbonyls 56 see
Azidophenyl glyoxal 60
Aziridines 118 122
Azo bond cleavage 63 66 212
Azodophenyl glyoxal 63
Azophenyldiamaleimide 109
Band 3 protein 240
BANPA (4-(Bromoaminoethyl)-3-nitrophenylazide) 173
BDC(1-Benzyl-3-(3-dimethylsminopropyl)carbodiimide) 196
BDI (Benzidine diisocyanate) 87
Benzidine diisocyanate (BDI) 87
Benzidine-3,3'-disulfonic acid 125
Benzophenone-4-iodoacetamide 176 226
Benzophenone-4-isothiocyanate 172
Benzophenone-4-maleimide 175
Benzophenones 63 184 see
Benzoquinone 95 103
Bimolecular nucleophilic substitution reaction 10 12
Bio-haloacetyl derivatives 111—114 see
Biotin see Avidin-biotin complex
Biotinyl-N-hydroxysuccinimide ester 258 259 286
Bis(-5'-Pyridoxalpyrophospho)methane 94
Bis(2,3-epoxypropyl)ether 119
Bis(2-(succimidyloxycarbonyloxy)ethyl)sulfone 222
Bis(2-(succinimido-oxycarbonyl)ethyl)sulfone (BSES) 84
Bis(2-succinimido-oxycarbonyloxy)ethyl)-sulfone (BSOCOES) 84
Bis(3,5-dibromosalicyl)fumarate 86 228
Bis(3,5-dibromosalicyl)succinate 86
Bis(3-nitro-4-fluorophenyl)sulfone 86
Bis(4-fluoro-3-nitro)sulfone 210
Bis(5'-pyridoxalpyrophospho)-2,3-glycerate 95
Bis(5'-pyridoxalpyrophosphol) 1,6-fructose 94
Bis(N-maleimido)-1,12-dodecane (BMD) 108
Bis(N-maleimido)-4,4'-bibenzyl (BMB) 109
Bis(N-maleimidomethyl)ether 108
Bis(N-succinimidyl)suberate 320
Bis(sulfo-N-succinimidyl)-doxyl-2-spiro-5'-azelate (BSSDA) 83
Bis(sulfo-N-succirarmdyl)-doxyl-2-spiro-4'-pirnelate (BSSDP) 83
Bis(sulfosuccinimidyl)suberate (BSSS) 82 240
Bis(ureido)prolylazobenzene 69
Bis-(-bromoacetyl--(2,4-dinitrophenyl)-lysylproyl)-ethylenediamine 113
Bis-(N-hydroxysuccinimide) 57 82 93 233
Bis-(N-maleimido)-1,8-octane (BMO) 108 223
Bis-(p-nitrophenyl ester) 90
Bis-(p-nitrophenyl) pimelate 91
Bis-(p-nitrophenyl) suberate 91
Bis-(p-nitrophenyl)adipate 90
Bis-alkyl halides 121—122
Bis-benzidine 124
Bis-epoxides 119 122 see
Bis-haloacetyl derivatives 121
Bis-imidoesters 1
Bis-imidoesters, acylation and 57
Bis-imidoesters, amino groups and 75—81 98
Bis-imidoesters, conformational analysis and 227
Bis-imidoesters, in ribosomal protein studies 224
Bis-imodoesters see also specific types
Bis-maleimides 104 107—111 120—121 223 see
Bis-maleimidohexane 53
Bis-N-maleimido-1,6-hexane 223
Bis-N-succinimidyls 81—85
Bis-nitrophenol esters 90—91 see
Bis-nitrophenyl esters 100 101 see
Bis-p-(2-carboxy-4'-azophenylisocyanate) 89
Bis-p-(2-carboxy-4'-azophenylisothiocyanate 89
Bis-PLP (,-Bis (5'-pyridoxal)-diphosphate 94
Bisdiazobenzine 58
Bisdiazohexane 123 124
Bisoxiranes 308
Bleomycin 279
BMB (Bis(N-maleimido)-4,4'-bibenzyl) 109
BMD (Bis(N-maleimido)-1,12-dodecane) 108
BMO (Bis(N-maleimido)-1,8-octane) 108 223
BMPS (N-Succinimidyl 3-maleimidopropionate) 148
Bromoacetate 23
Bromoacetylbromide 310
Bromoacetylcellulose 296
Bromotrifluoroacetone 30 32
BSES (Bis(2-(succinimido-oxycarbonyl)ethyl)sulfone) 84
BSOCOES (Bis(2-succinimido-oxycarbonyloxy)ethyl)-sulfone) 84
BSOPT(N,N’-Bis(3-succinimidyIoxy-carbonylpropyl) tartaramide) 85
BSSDA(Bis(sulfo-N-succinimidyl)-doxyl-2-spiro-5'-azelate) 83
BSSDP(Bis(sulfo-N-succinirnidyl)-doxyl-2-spiro-4'-pimelate) 83
BSSS (Bis(sulfosuccinimidyi)-suberate) 82 240
Bu-succinimidyls 98—99
Calcium-activated neutral proteinase 239
Calmodulin 227
Carbamate bonds 307
Carbenes 183
Carbodiimides in amide formation 23
Carbodiimides in immunoconjugate preparation 254
Carbodiimides in immunotoxin preparation 271
Carbodiimides, carboxyl group reactions and 39 123 195 199 204
Carbodiimides, photoactivatable reagents and 185
Carbodiimides, protein immobilization and 296 300 301 303 305
Carbohydrate prosthetic groups 25—28
Carbohydrates 8 130
Carbohydrates, immunotoxin coupling and 279
Carbohydrates, periodate activation of 27 37
Carbohydrates, s-triazines and 122
Carbohydrates, solid matrices and 312
Carbohydrates, zero-length cross-linkers and 203
Carbonyl bis(L-methionine p-nitrophenyl ester) CBMNPE 91
Carbonyl chloride 56
Carbonyl group reagents, heterobifunctional 161—162 182
Carbonyl groups 35
Carbonyldiimidazole 195 197 200 202 303 305 307
Carboxamide groups 177 185
Carboxyl group reagents 39 40
Carboxyl group reagents, heterobifunctional 161 183
Carboxyl group reagents, homobifunctional 123 124
Carboxyl group reagents, zero-length 195—204
Carboxyl groups 8 10 see
Carboxyl groups, acylation and 37
Carboxyl groups, pH effect on 12
Carboxyl groups, photoactivatable reagent anchoring by 177 185
Carboxyl groups, solid matrix activation of 296 299 300
Carboxylation 23
Carboxylic acids in solid matrices 296 299
Carboxylic acids, acylation and 53
Carboxylic acids, amine conversion to 16—17
Carboxylic acids, thiol conversion to 23
Carboxymethyldextran 279
CBMNPE (Carbonyl bis(L-methionine p-nitrophenyl ester)) 91
Cellulose 295 297
CETD (N,N’-bis2-carboximidoethyl) tartarimide dimethyl ester) 80
Chemoimmunoconjugates 267
Chitin 295
Chlorambucil-N-hydroxy succinimide 154
Chlorambucin 277—278
Chloramine T 68
Chloroacetate 23 27
Chlorocarbonate 305
Chloroformates 200 201 303 307
Chloromethyl 296
Chromate 321
Chromatin 319
Chromatography 1
Chromophoric probes 245
Chromotography 213
Chymotrypsin 14 16 42 45 121
Chymotrypsinogen 15
cis-DDP see cis-Dichlorodiaminoplatinum
cis-Dichlorodiaminoplatinum (cis-DDP) 127 132 228 319 321
Cleavage 180 212—214
Cleavage reagents 63—67
CMC see 1-Cyclohexyl-3-(2-morphylinyl-4-ethyl) carbodiimide
| CMTD(N,N’-Bis2-carboximidomethyl)tartarimide dimethyl ester) 80
Cobra venom factor (CVF) 267—269
Cobra venom factor , carbohydrate coupling and 279
Cobra venom factor , thioether linkage and 275—277
Collagen 297
Collisional cross-linking 233 234
Conformational analysis of membrane proteins 240
Conformational analysis of soluble proteins 227—228
Contractile protein system 225—226
Crabescein 107
Creatine kinase 239
CuP (Cupric di(1,10-phenanthroline)) 198 203
Cupric 1,10-phenanmrolinate 223
Cupric di(1,10-phenanthroline) (CuP) 198 203
CVF see Cobra venom factor
Cyanogen bromide 42 296 298—300 302
Cyanuric chloride 116 122 254 258 307
Cyssor reagent 182
Cystamine 272—273 286
Cysteine 7 8 11 12 17
Cysteine in photoactivatable reagents 185
Cysteine, carboxylation and 23
Cysteine, diazonium and 40
Cysteine, pH effect on 13
Cystine 23
Cytosine arabinoside 279
DABP (4,4'-Diazidobiphenyl) 129
DAN (1,5-Diazidonaphthalene) 129
Daunomycin 279 286 287
DCC (Dihexylcarbodiimide) 196
DDDP (Dimethyl 3,3'-dimethylenedioxy)-dipropion-imidate) 78
Deimethyl 3,3'-(diethyletherdioxy)-dipropionimidate 78
DEM (Diethyl malonimidate) 76
Dextran 279 297
Dextran-hydrazide 279
DFBN see Difluoro-2
Di(2-chloroethyl)-p-methoxyphenylamine 115
Di(2-chloroethyl)methylamine 114
Di(2-chloroethyl)sulfide 114
Di(2-chloroethyl)sulfone 114
Dialdehydes 92—95 101 see
Dialkyl halides 1 114—115
Diazo reagents 183
Diazoacetamides 39 see
Diazoacetates 31 39 see
Diazoacetyl groups 54 182
Diazonium 27 28 31 40 58 300
Diazotization 302
DIBAB (-(2,4-Dinitrophenyl)-a-bromoacetyl-L-diaminobutyric acid bromoacetylhydrazine) 112
Dibromoacetone 53
Dicarbonyl compounds 31
Dicarboxylic acids 16—17 53
Direct coupling 286
Disguised reagents 188
Disuccinimidyl tartarate (DST) 66 85 223
Disuccinimidylsuberate (DSS) 81 236
Disuccmimidyl-(N,N’diacerylhomocys-tine) 85
Disulfide bonds 16 35 98
Disulfide bonds, amino acid analysis and 214
Disulfide bonds, cleavage of 63 212 215
Disulfide bonds, heterobifunctional reagents and 182 185
Disulfide bonds, immunoconjugates and 251 252
Disulfide bonds, immunotoxins and 268—274
Disulfide bonds, zero-length cross-linkers and 195 198 203
Disulfide forming reagents 58 104 106—107 120
Disulfides 31 32—33 see
Disulfonic acids 53
Dithiobis(ethylmethylamidoethylmethylaminomethyltrimethylpsoralen) 319
Dithiobis(N-succinimidylpropionate) 320
Dithiobis(succinimidyl propionate) (DSP) 20 21 209 223 224
Dithiobis(succinimidyl propionate) in membrane protein studies 237—239
Dithiobis(succinimidyl propionate), protein immobilization and 310
Dithiobis(sulfosuccinimidyl propionate) 223
Dithiodiglycolic acid 20
Dithioerythritol 16 63
Dithiols 184 see
Dithionite 64 see
Dithiopyridine 252
Dithiothreitol (DTT) 16 20 21 33 63
Dithiothreitol, immunoconjugates and 251 254
Dithiothreitol, immunotoxins and 268 269 276 286
Divinylsulfone 119 122 123 308 310
DMA (Dimethyl adipimidate) 77 223 228
DMDP (Dimethyl 3,3'-(methylenedioxy)-dipropion-imidate) 78
DMG (Dimethyl glutarimidate) 77
DMM (Dimethyl malonimidate) 76
DMP (Dimethyl pimelimidate) 77 227
DMS see Dimethyl suberimidate
DMSC (Dimethyl succinimidate) 76
DMSDS (4,4'-Dimaleimidylstilbene-2,2'-disulfonic acid) 110
DNA cross-linking 319
DNA probes 2 320
DNA-protein conjugates 320—321
DNFA (2,4-Dinitro-5-fluorophenylazide) 172
DNFB see Dinitrofluorobenzene
DNP-RAT (Dinitrophenyl-1-tyrosine-p-azoben-zenearsenate) 130 132
DODP (Dimethyl 3,3'-oxydipropionimidate) 78
Dopamine receptors 235
DPEM see N-(-(4-Diazophenyl)ethyl)maleimide
DSP see Dithiobis(succinimidylpropionate)
DSS (Disuccinimidylsuberate) 81 236
DST (Disuccinimidyl tartarate) 66 85 223
Dt see Diphtheria toxin
DTBB (Dimethyl 4,4'-dithiobisbutyrimidate) 79
DTBE (Dimethyl 7,7'-dithiobisenanthimidate) 79
DTBP see Dimethyl 3
DTBV (Dimethyl 5,5'-dithiobisvalerimidate) 79
DTDP (Dimethyl 3,3'-(tetramethylenedioxy)-dipropionimidate) 78
DTNB (5,5'-Dithiobis-(2-nitrobenzoic acid)) see Ellman's reagent
DTPA see 4
DTPC (2-Diazo-3,3,3-trifluropropionyl chloride) 172
DTSSP (3,3'-Dithiobis(sulfosuccinimidylpropionate)) 85 310
DTT see Dithiothreitol
EA (Ethyl acetimidate) 75 76
EAC (1-Ethyl-3-(4-azonia-4,4-dimethylpentyl)carbodiimide iodide) 196 199
EADB (Ethyl-(4-azidophenyl)-1,4-dithiobutyrimidate) 171 238
EBC (Erythreitolbiscarbonate) 95
EDC see 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide
EEDQ (N-(Ethoxycarbonyl)-2-ethoxy-1,2-dihydroquinoline) 197 202 258
Effector-activating (Fc) fragments 248 249
EGS see Ethyleneglycol bis(succinimidylsuccinate)
EIA see Enzyme immunoassays
Electrophilicity 40 51 52 55 58
Electrophoresis 213
ELISA (Enzyme-linked immunosorbent assay) 2 245
Ellman's reagent 30 33 273—275
EMIT see Enzyme multiplied immunoassay technique
env gene 238
Enzyme immunoassay (EIA) 245 248—249 260
Enzyme multiplied immunoassay technique (EMIT) 245 247 248
Enzyme-antibody conjugates 245—247
Enzyme-antibody conjugates, components of 248—249
Enzyme-antibody conjugates, preparation of 250—258
Enzyme-antigen conjugates 245—247 259—260
Enzyme-linked immunosorbent assay (ELISA) 2 245
Enzymes 1 see
Enzymes, as zero-length cross-linkers 203—204
Enzymes, protein coupling and 259—260
Epichlorohydrin 158 180 310 311
Epidermal growth factor (EFG) 269 272
Equilibrium transfer alkylating cross-linkers (ETAC) 180—181
Erythreitolbiscarbonate (EBC) 95 104
Ester bond cleavage 63 66 213
ETAC (Equilibrium transfer alkylating cross-linkers) 180—181
Ethoxyformic anhydride 42
Ethyl acetimidate (EA) 75 76
Ethyl bromoacetimidate 156
Ethyl chloroacetimidate 156
Ethyl iodoacetimidate 156
Ethyl N-(5-azido-2-nitrobenzoyl)aminoacetimidate (ABNA) 170
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