Boyer R. — Modern Experimental Biochemistry :: Электронная библиотека попечительского совета мехмата МГУ

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Boyer R. — Modern Experimental Biochemistry

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Название: Modern Experimental Biochemistry

Автор: Boyer R.

Аннотация:

The latest edition of this successful text provides readers with a modern and complete experience in experimental biochemistry. Part I, Theory and Experimental Techniques, provides in-depth theoretical discussion organized around important techniques. A valuable reference for instructors and students, it's particularly useful to instructors who prefer to use their own customized experiments. Part II, Experiments, offers optimum flexibility through 15 tested experiments designed to accommodate the capabilities of laboratories and students at most four-year schools. Alternate methods are suggested and labs may be divided into manageable hour segments. The book offers the latest safety and environmental precautions in each experiment to inform students and instructors of potential hazards and proper disposal of materials. For anyone interested in science.

Язык:

Рубрика: Химия/

Статус предметного указателя: Готов указатель с номерами страниц

ed2k: ed2k stats

Издание: third edition

Год издания: 2000

Количество страниц: 475

Добавлена в каталог: 29.11.2005

Операции: Положить на полку | Скопировать ссылку для форума | Скопировать ID

Предметный указатель

$A_{260/280}$ of DNA      404 412
$A_{260}$ of nucleic acids      46 74 77 404 409 410 412
$A_{280/260}$ method      45 47 267 275 276
$A_{280}$ of proteins      44 45 74 77 266 274 275 296
      see Michaelis constant
      see Isoelectnc point
      see Relative mobility
      see Deoxynbonucleic acid melting
$V_{max}$       280—283 297—298 372 392 see
$\alpha$ -Lactalbumin, purification and characterization      220 257—277
$\beta$ -Carotene      334—335 337
$\lambda$ phage      see Deoxynbonucleic acid
$\sigma$ -phthalaldehyde      230
2,4-Dinitrofluorobenzene (Sanger reagent)      231
3,4-Dihydroxyphenylalanine (DOPA)      290—301
4-N, N-Dimethylamino-azobenzene-4'-isothiocyanate (DABITC)      231 232—233
9-Fluorenylmethyl chloroformate (FMOC), capillary electrophoresis      130 131 230—240
9-Fluorenylmethyl chloroformate (FMOC), high performance liquid chromatography      230 237
9-Fluorenylmethyl chloroformate (FMOC), reaction with ammo acids      230 237 240
Abbreviations, amino acids      229 445
Abbreviations, table of      445 447—448
Absorption      see Spectrophotometry Absorption coefficient
Absorption of $\alpha$ -lactalbumin      275—276
Absorption of chlorophyll      335—338 348
Absorption of DNA      412
Absorption of dopachrome      296—297
Absorption of NADH      368
Absorption of tyrosinase      152 296
Absorption of tyrosine      145
Absorption of uracil      151
Absorption, calculation of      145
Accuracy      see Statistical analysis Acetone
Accuracy , safe handling      311 339
Accuracy in cleaning      10—11
Accuracy in gel chromatography      87
Accuracy in photosynthetic pigment extraction      333—343
Accuracy in protein extraction      263
Acetic acid, MSDS      4 5 7
Acid-base reactions      29—37
Acids, table of      443
Acndine orange      163
Acrylamide      see also Polyacrylamide
Acrylamide, electrophoresis polymerization      113—121 272—273 389 391
Acrylamide, safe use      116 133 272 327 392
Activity, enzymatic      40 258 285—287 368—369 372 390 395 431—434
Activity, radioisotopes      175—176
Activity, thermodynamic      32
Adenine      399 401 404
Affinity chromatography of $\alpha$ -lactalbumin      264—277
Affinity chromatography of enzymes      99 100 101 106
Affinity chromatography, activated solid supports      100—106
Affinity chromatography, chaotropic agents      105—106
Affinity chromatography, elution      104—106
Affinity chromatography, group specific adsorbents      102—104
Affinity chromatography, immobilized metal ion      103 104 264—265
Affinity chromatography, ligands      99 101—104
Affinity chromatography, principles of      99—106
Affinity chromatography, procedure      104—106 271—272
Affinity chromatography, protein purification      99—106 257—277
Affinity chromatography, purification of $\alpha$ -chymotrypsin      106
Agarose gel electrophoresis      see also Electrophoresis
Agarose gel electrophoresis of DNA fragments      122—126 431—442
Agarose gel electrophoresis of plasmid DNA      122—126 415—429
Agarose gel electrophoresis, principles of      122—126
Agarose gel electrophoresis, procedures      133—138 415—428 431—442
Albumin, binding of phenol red      243—256
Albumin, standard protein      43 44 365
Albumin, transport properties      249—250
Amino acids, abbreviations      229 445
Amino acids, analysis      113 227—242
Amino acids, capillary electrophoresis      131 227—242
Amino acids, chromatography      61 69 76 88 92 228—229
Amino acids, detection of      64 229
Amino acids, DNP derivatives      231
Amino acids, FMOC derivatives      230 237
Amino acids, HPLC analysis      88 92 227—242
Amino acids, isoelectric points      445
Amino acids, nuclear magnetic resonance      164—166
Amino acids, pK values      445
Amino acids, sequence in peptides      227—242
Amino acids, structure      228
Aminomethyl coumann (AMC)      162 163
Amlino-8-naphthalene sulfonic acid (ANS)      162 163
Ammonium persulfate      113 133 268 272
Ammonium sulfate      262—263
Ampholytes      129—130 131
Analysis      see also Assay
Analysis by electrophoresis      111—138 257—277 321—331 431—442
Analysis of ammo acids      64 227—242
Analysis of blood      321—331 371—387
Analysis of carbohydrates      64 69 88 94
Analysis of chlorophyll      333—343 348—349
Analysis of dipeptides      227—242
Analysis of DNA      46—48 399—414 431—442
Analysis of glycoproteins      321—331
Analysis of lipids      64 303—319 371—387
Analysis of mitochondria      357—370
Analysis of photosynthetic pigments      333—343
Analysis of plasmids      415—429 431—442
Analysis of serum proteins      321—331
Analysis of triacylglycerols      303—319
Answers to Study Problems      454—465
Antibody      99 104 132 323 324 325 Immunoabsorption Western
Antigen      104 132 323
Ascorbic acid, measurement of      371—387
Ascorbic acid, structure      376
Assay      see also Analysis of ascorbic acid
Assay , enzyme      40 279—301 363 391—392
Assay , fixed-time      287—289 394
Assay , kinetic      287—289 391—392
Assay of chlorophyll      335—338 348—349
Assay of cholesterol      371—387
Assay of DNA      46—48 399—414 415—429
Assay of glycoproteins      321—331
Assay of malate dehydrogenase      363 366—367
Assay of monoamine oxidase      363 367
Assay of nucleic acids      46—48 399—414 431—442
Assay of proteins      41—45 227—242 258
Assay of tyrosinase      279—301
Atherosclerosis      373
Atomic masses, table of      451—452
ATP      165 167 346—348 358
Autoradiography      122 135—136 183—184
Average      see Statistical analysis Azide
Average, enzyme inhibitor      291
Average, preservative      79 84
Average, safe use      381
Background radiation      183 see also Radioactivity Bacterial cells
Background radiation, DNA isolation      399—414
Background radiation, growth of      423—429
Background radiation, hybrid DNA in      259 415—429
Background radiation, plasmid isolation      425—429
Background radiation, safe handling      409
Background radiation, sources of proteins      258—259
Bactenophage DNA      416
BamHI      433 435 437—442
Bases, pairing in DNA      399 400 405
Bases, table of      443
BCA protein assay      42 44
Bed volume      70 80
Beer — Lambert equation      144—146 157 267 275—276 296—297
Binding      see Proteins ligand Deoxynbonucleic
Biochemical buffers in electrophoresis      117—119 433—434
Biochemical buffers, ammo acids      37
Biochemical buffers, Good's      35—37
Biochemical buffers, phosphate      35 261
Biochemical buffers, pK      33 36 444
Biochemical buffers, principles of      32—33
Biochemical buffers, selection of      34—37 261 359
Biochemical buffers, structure      36

Biochemical buffers, table of      36 433 444
Biochemical buffers, Tris      36 37 261
Biochemical buffers, useful range      34
Biochemical buffers, zwitterionic      35—37
Bioinformatics      211—225
Biological databases      214 215 219 224
Biosensors      39—41
biotechnology      389
Bisbenzimidazole, DNA analysis      46 47
Biuret protein assay      41 42 43 364—365
Blood, cholesterol      371—387
Blood, safe handling      379 381
Blood, serum proteins      321—331
Blotting      see also Electrophoresis
Blotting of nucleic acids      136—138
Blotting of proteins      136—138 321—331
Blotting, membranes      136 322
Blotting, Northern      136 329
Blotting, Southern      136 329
Blotting, Western      132 136 321—331
Bookmark      214 224
Bovine plasma gamma globulin      44 45 269 274 275
Bovine serum albumin (BSA)      43 44 243—256 327 365
Bradford protein assay      42 43—44 152—153 267 269 273—274 275
Bromophenol blue      134 268 326 328 437
Browser      213 224
buffers      see Biochemical buffers
Calibration curve, Biuret protein assay      43
Calibration curve, Bradford protein assay      45 152 267 273—274 275
Calibration curve, fatty acid methyl esters      315—316
Calibration curve, gel filtration of proteins      86—87
Calibration curve, Lowry protein assay      42 43
Calibration curve, nucleic acid electrophoresis      123 138 421—422 440
Calibration curve, protein electrophoresis      120 138
Calvin cycle      347
Capillary electrophoresis      130—131 228 230 234—242
Carbohydrates      37 64 304 306 321—331
Carboxypeptidases A and B      233 234
Carotenoids      333—343
Caseins      265 323
Cell lysis      259—261 359 402 403 409
Cellulose, chromatography supports      61 62 76 79
Cellulose, electrophoresis supports      113
Centrifugal vacuum concentration      55 237
Centrifugation      see also Centrifuges Rotors
Centrifugation, analytical      199 202
Centrifugation, applications      201—206 357—370
Centrifugation, centrifugal force      189—190
Centrifugation, density gradient      205—206
Centrifugation, differential      201—204 258—259 262
Centrifugation, instrumentation      193—201
Centrifugation, isopycnic      205—206
Centrifugation, preparative      199 201
Centrifugation, principles      189—193
Centrifugation, relative centrifugal force      190
Centrifugation, sedimentation equilibrium      204
Centrifugation, vacuum concentration      55
Centrifugation, velocity sedimentation      201
Centrifugation, zonal      205
Centrifuge microfilter      50 51 see
Centrifuges      see also Centnfugation Rotors
Centrifuges, analytical      199 202
Centrifuges, care and maintenance      206—207
Centrifuges, high-speed      195—198
Centrifuges, low-speed      193—194
Centrifuges, micro      195—198
Centrifuges, preparative      199 201
Centrifuges, safe use      206—207
Centrifuges, ultra      198—201
Cesium salts      205—206
Chaotropic agents      105—106 see
Chemical Abstracts      218 see
Chemiluminescence      136 324
Chemiosmotic coupling hypothesis      347—348
Chloramphenicol      415 418 419 422 425
Chloroform, safe use      313
Chlorophylls, absorption spectra      336
Chlorophylls, extraction      333—343
Chlorophylls, measurement      336—338 348—349
Chlorophylls, structure      334
Chloroplasts, chlorophyll content      333 348—349 351
Chloroplasts, definition      347
Chloroplasts, preparation of      206 351
Chloroplasts, proton uptake      345—355
Cholesterol, blood analysis      371—387
Cholesterol, esters      373—374
Cholesterol, HDL      373 375 382 384
Cholesterol, LDL      373 375 384
Cholesterol, normal serum levels      376
Cholesterol, structure      304 374
Chromatofocusing      79
Chromatography of $\alpha$ -lactalbumin      257—277
Chromatography of ammo acids      61 69 76 88 92 227—242
Chromatography of fatty acid methyl esters      69 308—319
Chromatography of lipids      303—319 333—343
Chromatography of photosynthetic pigments      333—343
Chromatography, value      62 63 240 307 314
Chromatography, adsorption      60—61 305
Chromatography, affinity      99—106 264 271—272
Chromatography, analytical      60
Chromatography, column      70—74 303—319
Chromatography, covalent      103
Chromatography, fast protein liquid      98—99
Chromatography, gas-liquid      65—69 229 303—319
Chromatography, gel-exclusion      79—87 243—256
Chromatography, high performance liquid      87—99 227—242
Chromatography, hydrophobic interaction      103
Chromatography, immobilized metal-ion affinity      103—104 264—277
Chromatography, immunoadsorption      104 321—331
Chromatography, ion-exchange      74—79 228
Chromatography, paper      61—65 227—242
Chromatography, partition      60—61
Chromatography, perfusion      106—107
Chromatography, planar      61—65 227—242 303—319
Chromatography, preparative      60
Chromatography, principles of      59—61
Chromatography, protein purification      70—107 264—265
Chromatography, thin-layer      61—65 227—242 303—319
Chromatography, two-dimensional      64 227—242
Cleaning centrifuge rotors      206—207
Cleaning cuvettes      11
Cleaning laboratory glassware      10—11
Cleaning pipets      18
Cleaning radioactive spills      185—186
Cloning      see Recombinant DNA
Column chromatography      70—74 see
Computer browser      213 224
Computer in biochemical research      211—225
Computer server      225
Computer, database directories      214
Computer, domain      213 224
Computer, freeware      213 224
Computer, home page      213 224
Computer, HTML      213 224
Computer, hyperlink      214 225
Computer, hypertext      213 225
Computer, internet      212 225
Computer, Java      225
Computer, modem      213 225
Computer, multimedia      225
Computer, search engine      214 225
Computer, URL      213 225
Computer, web site      213—214 225
Computer, WWW      213 225
Concanavalm A-HRP conjugate      324—331
Concentration, solution      11—13
Contact lenses, safety      7
Coomassie Blue dye      43 44 134 273 321 328
Cooperativity      248
Cuvettes, cleaning      11

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