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Boyer R. Ч Modern Experimental Biochemistry
Boyer R. Ч Modern Experimental Biochemistry



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Ќазвание: Modern Experimental Biochemistry

јвтор: Boyer R.

јннотаци€:

The latest edition of this successful text provides readers with a modern and complete experience in experimental biochemistry. Part I, Theory and Experimental Techniques, provides in-depth theoretical discussion organized around important techniques. A valuable reference for instructors and students, it's particularly useful to instructors who prefer to use their own customized experiments. Part II, Experiments, offers optimum flexibility through 15 tested experiments designed to accommodate the capabilities of laboratories and students at most four-year schools. Alternate methods are suggested and labs may be divided into manageable hour segments. The book offers the latest safety and environmental precautions in each experiment to inform students and instructors of potential hazards and proper disposal of materials. For anyone interested in science.


язык: en

–убрика: ’ими€/

—татус предметного указател€: √отов указатель с номерами страниц

ed2k: ed2k stats

»здание: third edition

√од издани€: 2000

 оличество страниц: 475

ƒобавлена в каталог: 29.11.2005

ќперации: ѕоложить на полку | —копировать ссылку дл€ форума | —копировать ID
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ѕредметный указатель
$A_{260/280}$ of DNA      404 412
$A_{260}$ of nucleic acids      46 74 77 404 409 410 412
$A_{280/260}$ method      45 47 267 275 276
$A_{280}$ of proteins      44 45 74 77 266 274 275 296
$K_M$      see Michaelis constant
$pH_I$      see Isoelectnc point
$R_f$      see Relative mobility
$T_m$      see Deoxynbonucleic acid melting
$V_{max}$      280Ч283 297Ч298 372 392 see
$\alpha$-Lactalbumin, purification and characterization      220 257Ч277
$\beta$-Carotene      334Ч335 337
$\lambda$ phage      see Deoxynbonucleic acid
$\sigma$-phthalaldehyde      230
2,4-Dinitrofluorobenzene (Sanger reagent)      231
3,4-Dihydroxyphenylalanine (DOPA)      290Ч301
4-N, N-Dimethylamino-azobenzene-4'-isothiocyanate (DABITC)      231 232Ч233
9-Fluorenylmethyl chloroformate (FMOC), capillary electrophoresis      130 131 230Ч240
9-Fluorenylmethyl chloroformate (FMOC), high performance liquid chromatography      230 237
9-Fluorenylmethyl chloroformate (FMOC), reaction with ammo acids      230 237 240
Abbreviations, amino acids      229 445
Abbreviations, table of      445 447Ч448
Absorption      see Spectrophotometry Absorption coefficient
Absorption of $\alpha$-lactalbumin      275Ч276
Absorption of chlorophyll      335Ч338 348
Absorption of DNA      412
Absorption of dopachrome      296Ч297
Absorption of NADH      368
Absorption of tyrosinase      152 296
Absorption of tyrosine      145
Absorption of uracil      151
Absorption, calculation of      145
Accuracy      see Statistical analysis Acetone
Accuracy , safe handling      311 339
Accuracy in cleaning      10Ч11
Accuracy in gel chromatography      87
Accuracy in photosynthetic pigment extraction      333Ч343
Accuracy in protein extraction      263
Acetic acid, MSDS      4 5 7
Acid-base reactions      29Ч37
Acids, table of      443
Acndine orange      163
Acrylamide      see also Polyacrylamide
Acrylamide, electrophoresis polymerization      113Ч121 272Ч273 389 391
Acrylamide, safe use      116 133 272 327 392
Activity, enzymatic      40 258 285Ч287 368Ч369 372 390 395 431Ч434
Activity, radioisotopes      175Ч176
Activity, thermodynamic      32
Adenine      399 401 404
Affinity chromatography of $\alpha$-lactalbumin      264Ч277
Affinity chromatography of enzymes      99 100 101 106
Affinity chromatography, activated solid supports      100Ч106
Affinity chromatography, chaotropic agents      105Ч106
Affinity chromatography, elution      104Ч106
Affinity chromatography, group specific adsorbents      102Ч104
Affinity chromatography, immobilized metal ion      103 104 264Ч265
Affinity chromatography, ligands      99 101Ч104
Affinity chromatography, principles of      99Ч106
Affinity chromatography, procedure      104Ч106 271Ч272
Affinity chromatography, protein purification      99Ч106 257Ч277
Affinity chromatography, purification of $\alpha$-chymotrypsin      106
Agarose gel electrophoresis      see also Electrophoresis
Agarose gel electrophoresis of DNA fragments      122Ч126 431Ч442
Agarose gel electrophoresis of plasmid DNA      122Ч126 415Ч429
Agarose gel electrophoresis, principles of      122Ч126
Agarose gel electrophoresis, procedures      133Ч138 415Ч428 431Ч442
Albumin, binding of phenol red      243Ч256
Albumin, standard protein      43 44 365
Albumin, transport properties      249Ч250
Amino acids, abbreviations      229 445
Amino acids, analysis      113 227Ч242
Amino acids, capillary electrophoresis      131 227Ч242
Amino acids, chromatography      61 69 76 88 92 228Ч229
Amino acids, detection of      64 229
Amino acids, DNP derivatives      231
Amino acids, FMOC derivatives      230 237
Amino acids, HPLC analysis      88 92 227Ч242
Amino acids, isoelectric points      445
Amino acids, nuclear magnetic resonance      164Ч166
Amino acids, pK values      445
Amino acids, sequence in peptides      227Ч242
Amino acids, structure      228
Aminomethyl coumann (AMC)      162 163
Amlino-8-naphthalene sulfonic acid (ANS)      162 163
Ammonium persulfate      113 133 268 272
Ammonium sulfate      262Ч263
Ampholytes      129Ч130 131
Analysis      see also Assay
Analysis by electrophoresis      111Ч138 257Ч277 321Ч331 431Ч442
Analysis of ammo acids      64 227Ч242
Analysis of blood      321Ч331 371Ч387
Analysis of carbohydrates      64 69 88 94
Analysis of chlorophyll      333Ч343 348Ч349
Analysis of dipeptides      227Ч242
Analysis of DNA      46Ч48 399Ч414 431Ч442
Analysis of glycoproteins      321Ч331
Analysis of lipids      64 303Ч319 371Ч387
Analysis of mitochondria      357Ч370
Analysis of photosynthetic pigments      333Ч343
Analysis of plasmids      415Ч429 431Ч442
Analysis of serum proteins      321Ч331
Analysis of triacylglycerols      303Ч319
Answers to Study Problems      454Ч465
Antibody      99 104 132 323 324 325 Immunoabsorption Western
Antigen      104 132 323
Ascorbic acid, measurement of      371Ч387
Ascorbic acid, structure      376
Assay      see also Analysis of ascorbic acid
Assay , enzyme      40 279Ч301 363 391Ч392
Assay , fixed-time      287Ч289 394
Assay , kinetic      287Ч289 391Ч392
Assay of chlorophyll      335Ч338 348Ч349
Assay of cholesterol      371Ч387
Assay of DNA      46Ч48 399Ч414 415Ч429
Assay of glycoproteins      321Ч331
Assay of malate dehydrogenase      363 366Ч367
Assay of monoamine oxidase      363 367
Assay of nucleic acids      46Ч48 399Ч414 431Ч442
Assay of proteins      41Ч45 227Ч242 258
Assay of tyrosinase      279Ч301
Atherosclerosis      373
Atomic masses, table of      451Ч452
ATP      165 167 346Ч348 358
Autoradiography      122 135Ч136 183Ч184
Average      see Statistical analysis Azide
Average, enzyme inhibitor      291
Average, preservative      79 84
Average, safe use      381
Background radiation      183 see also Radioactivity Bacterial cells
Background radiation, DNA isolation      399Ч414
Background radiation, growth of      423Ч429
Background radiation, hybrid DNA in      259 415Ч429
Background radiation, plasmid isolation      425Ч429
Background radiation, safe handling      409
Background radiation, sources of proteins      258Ч259
Bactenophage DNA      416
BamHI      433 435 437Ч442
Bases, pairing in DNA      399 400 405
Bases, table of      443
BCA protein assay      42 44
Bed volume      70 80
Beer Ч Lambert equation      144Ч146 157 267 275Ч276 296Ч297
Binding      see Proteins ligand Deoxynbonucleic
Biochemical buffers in electrophoresis      117Ч119 433Ч434
Biochemical buffers, ammo acids      37
Biochemical buffers, Good's      35Ч37
Biochemical buffers, phosphate      35 261
Biochemical buffers, pK      33 36 444
Biochemical buffers, principles of      32Ч33
Biochemical buffers, selection of      34Ч37 261 359
Biochemical buffers, structure      36
Biochemical buffers, table of      36 433 444
Biochemical buffers, Tris      36 37 261
Biochemical buffers, useful range      34
Biochemical buffers, zwitterionic      35Ч37
Bioinformatics      211Ч225
Biological databases      214 215 219 224
Biosensors      39Ч41
biotechnology      389
Bisbenzimidazole, DNA analysis      46 47
Biuret protein assay      41 42 43 364Ч365
Blood, cholesterol      371Ч387
Blood, safe handling      379 381
Blood, serum proteins      321Ч331
Blotting      see also Electrophoresis
Blotting of nucleic acids      136Ч138
Blotting of proteins      136Ч138 321Ч331
Blotting, membranes      136 322
Blotting, Northern      136 329
Blotting, Southern      136 329
Blotting, Western      132 136 321Ч331
Bookmark      214 224
Bovine plasma gamma globulin      44 45 269 274 275
Bovine serum albumin (BSA)      43 44 243Ч256 327 365
Bradford protein assay      42 43Ч44 152Ч153 267 269 273Ч274 275
Bromophenol blue      134 268 326 328 437
Browser      213 224
buffers      see Biochemical buffers
Calibration curve, Biuret protein assay      43
Calibration curve, Bradford protein assay      45 152 267 273Ч274 275
Calibration curve, fatty acid methyl esters      315Ч316
Calibration curve, gel filtration of proteins      86Ч87
Calibration curve, Lowry protein assay      42 43
Calibration curve, nucleic acid electrophoresis      123 138 421Ч422 440
Calibration curve, protein electrophoresis      120 138
Calvin cycle      347
Capillary electrophoresis      130Ч131 228 230 234Ч242
Carbohydrates      37 64 304 306 321Ч331
Carboxypeptidases A and B      233 234
Carotenoids      333Ч343
Caseins      265 323
Cell lysis      259Ч261 359 402 403 409
Cellulose, chromatography supports      61 62 76 79
Cellulose, electrophoresis supports      113
Centrifugal vacuum concentration      55 237
Centrifugation      see also Centrifuges Rotors
Centrifugation, analytical      199 202
Centrifugation, applications      201Ч206 357Ч370
Centrifugation, centrifugal force      189Ч190
Centrifugation, density gradient      205Ч206
Centrifugation, differential      201Ч204 258Ч259 262
Centrifugation, instrumentation      193Ч201
Centrifugation, isopycnic      205Ч206
Centrifugation, preparative      199 201
Centrifugation, principles      189Ч193
Centrifugation, relative centrifugal force      190
Centrifugation, sedimentation equilibrium      204
Centrifugation, vacuum concentration      55
Centrifugation, velocity sedimentation      201
Centrifugation, zonal      205
Centrifuge microfilter      50 51 see
Centrifuges      see also Centnfugation Rotors
Centrifuges, analytical      199 202
Centrifuges, care and maintenance      206Ч207
Centrifuges, high-speed      195Ч198
Centrifuges, low-speed      193Ч194
Centrifuges, micro      195Ч198
Centrifuges, preparative      199 201
Centrifuges, safe use      206Ч207
Centrifuges, ultra      198Ч201
Cesium salts      205Ч206
Chaotropic agents      105Ч106 see
Chemical Abstracts      218 see
Chemiluminescence      136 324
Chemiosmotic coupling hypothesis      347Ч348
Chloramphenicol      415 418 419 422 425
Chloroform, safe use      313
Chlorophylls, absorption spectra      336
Chlorophylls, extraction      333Ч343
Chlorophylls, measurement      336Ч338 348Ч349
Chlorophylls, structure      334
Chloroplasts, chlorophyll content      333 348Ч349 351
Chloroplasts, definition      347
Chloroplasts, preparation of      206 351
Chloroplasts, proton uptake      345Ч355
Cholesterol, blood analysis      371Ч387
Cholesterol, esters      373Ч374
Cholesterol, HDL      373 375 382 384
Cholesterol, LDL      373 375 384
Cholesterol, normal serum levels      376
Cholesterol, structure      304 374
Chromatofocusing      79
Chromatography of $\alpha$-lactalbumin      257Ч277
Chromatography of ammo acids      61 69 76 88 92 227Ч242
Chromatography of fatty acid methyl esters      69 308Ч319
Chromatography of lipids      303Ч319 333Ч343
Chromatography of photosynthetic pigments      333Ч343
Chromatography, $R_f$value      62 63 240 307 314
Chromatography, adsorption      60Ч61 305
Chromatography, affinity      99Ч106 264 271Ч272
Chromatography, analytical      60
Chromatography, column      70Ч74 303Ч319
Chromatography, covalent      103
Chromatography, fast protein liquid      98Ч99
Chromatography, gas-liquid      65Ч69 229 303Ч319
Chromatography, gel-exclusion      79Ч87 243Ч256
Chromatography, high performance liquid      87Ч99 227Ч242
Chromatography, hydrophobic interaction      103
Chromatography, immobilized metal-ion affinity      103Ч104 264Ч277
Chromatography, immunoadsorption      104 321Ч331
Chromatography, ion-exchange      74Ч79 228
Chromatography, paper      61Ч65 227Ч242
Chromatography, partition      60Ч61
Chromatography, perfusion      106Ч107
Chromatography, planar      61Ч65 227Ч242 303Ч319
Chromatography, preparative      60
Chromatography, principles of      59Ч61
Chromatography, protein purification      70Ч107 264Ч265
Chromatography, thin-layer      61Ч65 227Ч242 303Ч319
Chromatography, two-dimensional      64 227Ч242
Cleaning centrifuge rotors      206Ч207
Cleaning cuvettes      11
Cleaning laboratory glassware      10Ч11
Cleaning pipets      18
Cleaning radioactive spills      185Ч186
Cloning      see Recombinant DNA
Column chromatography      70Ч74 see
Computer browser      213 224
Computer in biochemical research      211Ч225
Computer server      225
Computer, database directories      214
Computer, domain      213 224
Computer, freeware      213 224
Computer, home page      213 224
Computer, HTML      213 224
Computer, hyperlink      214 225
Computer, hypertext      213 225
Computer, internet      212 225
Computer, Java      225
Computer, modem      213 225
Computer, multimedia      225
Computer, search engine      214 225
Computer, URL      213 225
Computer, web site      213Ч214 225
Computer, WWW      213 225
Concanavalm A-HRP conjugate      324Ч331
Concentration, solution      11Ч13
Contact lenses, safety      7
Coomassie Blue dye      43 44 134 273 321 328
Cooperativity      248
Cuvettes, cleaning      11
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