Boyer R. — Modern Experimental Biochemistry :: Электронная библиотека попечительского совета мехмата МГУ

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Boyer R. — Modern Experimental Biochemistry

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Название: Modern Experimental Biochemistry

Автор: Boyer R.

Аннотация:

The latest edition of this successful text provides readers with a modern and complete experience in experimental biochemistry. Part I, Theory and Experimental Techniques, provides in-depth theoretical discussion organized around important techniques. A valuable reference for instructors and students, it's particularly useful to instructors who prefer to use their own customized experiments. Part II, Experiments, offers optimum flexibility through 15 tested experiments designed to accommodate the capabilities of laboratories and students at most four-year schools. Alternate methods are suggested and labs may be divided into manageable hour segments. The book offers the latest safety and environmental precautions in each experiment to inform students and instructors of potential hazards and proper disposal of materials. For anyone interested in science.

Язык:

Рубрика: Химия/

Статус предметного указателя: Готов указатель с номерами страниц

ed2k: ed2k stats

Издание: third edition

Год издания: 2000

Количество страниц: 475

Добавлена в каталог: 29.11.2005

Операции: Положить на полку | Скопировать ссылку для форума | Скопировать ID

Предметный указатель

Cuvettes, fluorescence      160
Cuvettes, transmission properties      148—149
Cuvettes, types of      148—149
Cytosine      399 401 404
Cytosol      201 202 259
Dansic acid      240
Dansyl ammo acids      227—242
Dansyl chloride, peptide analysis      162 163 227—242
Dansyl chloride, safe use      238
Dansyl chloride, structure      231
Database directories      214—215
Denaturants      119—121 403
Density gradients      205—206 see
Deoxynbonucleic acid, $\lambda$ phage      431—442
Deoxynbonucleic acid, absorbance spectrum      154
Deoxynbonucleic acid, analysis of      46—48 111—140 399—414 431—442
Deoxynbonucleic acid, base composition      399
Deoxynbonucleic acid, binding of polyamines      406—407 411—414
Deoxynbonucleic acid, bisbenzimidazole assay      46 47
Deoxynbonucleic acid, blotting      136—137
Deoxynbonucleic acid, characterization      399—414 415—429 431—442
Deoxynbonucleic acid, chromosomal      399—414
Deoxynbonucleic acid, cleavage by enzymes      124 431—442
Deoxynbonucleic acid, conformation      124—127
Deoxynbonucleic acid, denaturation      122 399—414
Deoxynbonucleic acid, detection with ethidium bromide      46 135 162 399—414
Deoxynbonucleic acid, Dipstick      46 47
Deoxynbonucleic acid, electrophoresis      111—140 415—429 431—442
Deoxynbonucleic acid, fingerprinting      124 432
Deoxynbonucleic acid, fluorescence assay      46 162 399—414
Deoxynbonucleic acid, hybridization      136 415—429
Deoxynbonucleic acid, intercalation      206 399 406—407
Deoxynbonucleic acid, isolation      206 399—414 415—429
Deoxynbonucleic acid, library      136 433—434
Deoxynbonucleic acid, ligase      416 418
Deoxynbonucleic acid, melting temperature      404—406 410
Deoxynbonucleic acid, plasmid      124 418—423 431—440
Deoxynbonucleic acid, recombinant      124 136 259 415—429 431—440
Deoxynbonucleic acid, sequencing      211 219 420 434
Deoxynbonucleic acid, spectrophotometnc assay      46 47 404 412
Deoxynbonucleic acid, stability      400—404
Deoxynbonucleic acid, structure and function      399—404
Deoxynbonucleic acid, supercoiled      124—126
Destaimng      134—136 273 328 see Staining
Detectors, absorbance spectrometry      146—147 149
Detectors, capillary electrophoresis      236
Detectors, column chromatography      73—74
Detectors, diode array      147 149
Detectors, fluorescence spectrometry      160—161
Detectors, gas chromatography      66 67
Detectors, high performance liquid chromatography      91 235—236
Detectors, radioactivity      176—183
Dextran      75 76 79 81 82 83
Dialysis      48—49 264
Dichlorophenolindophenol (DCIP)      350 371 377—378 382—384
Difference spectroscopy      155—156
Differential centnfugation      201—204 258—259 262 358—360 see
Direct linear plot      281 282 283 see kinetics
Disc gel electrophoresis      see Electrophoresis
DNA      see Deoxynbonucleic acid
DOPA      see Dihydroxyphenylalanine
Dopachrome      290—301
Double helix      399—400 402 415 see
Dyes      43 44 134—136 162—163 349 350 see
e-mail      213 224
Eadie — Hofstee equation      298
EcoRI      419 432 433 435 438 see
Edman reagent      see Phenyhsothiocyanate
Eisenthal and Cornish-Bowden plot      see Direct linear plot
Electrodes, ion-selective      39—41
Electrodes, oxygen      37—39
Electrodes, pH      30—32
Electromagnetic spectrum      142
Electrophoresis of $\alpha$ -lactalbumin      267—277
Electrophoresis of nucleic acids      121—122 415—429 431—442
Electrophoresis of proteins      113—121 257—277 321—331
Electrophoresis, agarose gel      122—126 415—429 431—442
Electrophoresis, blotting      136 321—331
Electrophoresis, bufferless precast gels      134
Electrophoresis, buffers      117—119 433 434
Electrophoresis, capillary      130—131 227—242
Electrophoresis, column gel      115
Electrophoresis, denaturing      119—121 267—277 321—331
Electrophoresis, disc gel      117—119
Electrophoresis, immuno      132
Electrophoresis, instrumentation      133—134
Electrophoresis, isoelectric focusing      127—130 258
Electrophoresis, methods      268—277 327—328 431—442
Electrophoresis, polyacrylamide      113—121 257—277 321—331
Electrophoresis, practical aspects      133—138
Electrophoresis, precast gels      133—134
Electrophoresis, principles of      111—112
Electrophoresis, pulsed field gel      126—127
Electrophoresis, reusable gels      134
Electrophoresis, safe use      133 272 327 427 439
Electrophoresis, SDS-PAGE      257—277 321—331
Electrophoresis, serum proteins      321—331
Electrophoresis, slab gel      115
Electrophoresis, staining      134—136 273 328
Electrophoresis, two-dimensional      130
Elements, table of      451—452
Eluotropic series      96—98 see
Elution      60 70 72—73 see
Elution volume      81 85 see
Enzymes      see also specific enzyme
Enzymes as diagnostic reagents      371—375
Enzymes in mitochondria      357—370
Enzymes, affinity chromatography      99 101 106
Enzymes, assay of      40 258 279—301 366—367 390 391—392
Enzymes, detection in electrophoresis      322
Enzymes, immobilized      389—397
Enzymes, inhibition      262 283—285 295 298
Enzymes, kinetics      279—289 391—392
Enzymes, purification      99 101 106 257—265
Enzymes, units of activity      285—287 392 434
Eppendorf pipets      see Pipets automatic
Error      see Statistical analysis
Ethidium bromide in electrophoresis      123 125 135 422
Ethidium bromide, assay of DNA      46 399—414
Ethidium bromide, detection of nucleic acids      46—48 123 125 135 162 399—414
Ethidium bromide, intercalation with nucleic acids      123 125 135 162 406—407
Ethidium bromide, safe use      135 410 439
Ethidium bromide, structure      163 406
Extraction of chlorophyll      333—343
Extraction of chloroplasts      345—355
Extraction of DNA      399—414
Extraction of lipids      303—319
Extraction of photosynthetic pigments      333—343
Extraction of proteins      257—267
FAME      see Fatty acids methyl
Fast protein liquid chromatography (FPLC)      see Chromatography
Fatty acids in nutmeg      303—319
Fatty acids in triacylglycerols      303—319
Fatty acids, analysis by GC      69 303—319
Fatty acids, methyl esters      308—319
File Transfer Protocol      213 224
Filtration      see Ultrafiltration
Fixed-time assay      287—289 394
Flammabihty      see Safety
Flavin mononucleotide (FMN), absorbance spectrum of      154
flowchart      9
Fluorescein      163
Fluorescence spectrophotometry, applications      161—163 406—407
Fluorescence spectrophotometry, cuvettes in      160
Fluorescence spectrophotometry, detectors      91 160—161
Fluorescence spectrophotometry, fluors      161—163
Fluorescence spectrophotometry, instrumentation      160—161
Fluorescence spectrophotometry, measurement of ammo acids      161—162 229—233
Fluorescence spectrophotometry, measurement of ligand binding      159 162 399—414
Fluorescence spectrophotometry, measurement of nucleic acids      46—48 406—414

Fluorescence spectrophotometry, principles      157—161
FMN      see Flavin mononucleotide
Freeze drying      see Lyophilization
French press      259
Gas-liquid chromatography of amino acids      69 229
Gas-liquid chromatography of fatty acids      308—319
Gas-liquid chromatography, compared to HPLC      88—89
Gas-liquid chromatography, instrumentation      65—67
Gas-liquid chromatography, principles      65—69
Gas-liquid chromatography, response correction factor      315—316
Gaussian distribution curve      22 see
Gel-exclusion chromatography in HPLC      95
Gel-exclusion chromatography in organic solvents      87
Gel-exclusion chromatography, applications      85—87 243—256
Gel-exclusion chromatography, compared to electrophoresis      113
Gel-exclusion chromatography, ligand-protein interactions      99—100 243—256
Gel-exclusion chromatography, principles      79—85
Gel-exclusion chromatography, purification of $\alpha$ -lactalbumin      263—277
Gel-exclusion chromatography, purification of proteins      79—87 263—267
Gel-exclusion chromatography, supports      81—84
Glycohpids      304 306
Glycoproteins      321—331
Good's buffers      see Biochemical buffers
Guanme      399 401 404
Hazardous Materials Identification System (HMIS)      4 6
Hemoglobin, absorption spectrum      145
Hemoglobin, difference spectrum      156 157
Henderson — Hasselbalch equation      33 35
Hexane, extraction of lipids      304—305 311—312
Hexane, safe handling      311
High-performance liquid chromatography      see also Chromatography
High-performance liquid chromatography in peptide analysis      227—242
High-performance liquid chromatography of ammo acid derivatives      92 229 235—242
High-performance liquid chromatography of proteins      94 98—99
High-performance liquid chromatography, gel-exclusion      95
High-performance liquid chromatography, gradient elution      97—98
High-performance liquid chromatography, instrumentation      89—92
High-performance liquid chromatography, ion-exchange      94—95
High-performance liquid chromatography, normal phase      93
High-performance liquid chromatography, perfusion      106—107
High-performance liquid chromatography, principles of      87—99
High-performance liquid chromatography, reverse-phase      94 99 106—107
High-performance liquid chromatography, solvents      89 96—98
Hinfl      432 435 see
histories      402 403
Home page      213 224
Homogenizers      259—261 359
HpaI      433 435 437 439 see
HTML      213 224
Human genome project      122 127 219
Hydrogen bonding      104 244 399 400 405
Hydrophobic interactions      103 104 244 405
Hypercholesterolemia      373
Hyperchromic effect      405
Immobilized enzymes      389—397
Immobilized metal-ion affinity chro-matography (IMAC)      103 104 264—265 see
Immunoadsorption      104 132 323
Immunoblotting      see Western blotting
Immunoelectrophoresis      132
Inhibition of enzyme activity      262 283—285 295 298 see
Intercalation      123 125 206 399 406 see Ethidium
Internet      212 225
Ion-exchange chromatography      see also Chromatography
Ion-exchange chromatography in HPLC      94—95 228
Ion-exchange chromatography of ammo acids      76 228
Ion-exchange chromatography of proteins      74—79 263—264
Ion-exchange chromatography, principles      74—79
Ion-exchange chromatography, resins      75—77
Isoelectnc focusing      127—130 131 258 264
Isoelectnc point      127 263
Isopropanol in lipid extraction      304—305 311—312
Isopropanol, safe handling      311
Kinetic assay      153 279—289 391—392 see
Laboratory, cleaning glassware      10—11
Laboratory, computer      211—225
Laboratory, general procedures      3—25
Laboratory, notebook      8—10
Laboratory, pipetting      13—18
Laboratory, safety      4—8 206—207
Laboratory, solution preparation      11—13
Laboratory, statistical analysis      18—25
Lectins      321 324—325
Library, abstracts      218
Library, computer-based searches      218—219
Library, DNA      136 433—434
Library, handbooks      217—218
Library, methodology references      217
Library, reference books      216
Library, research journals      216—217
Library, reviews      216
Library, search aids      218—219
Library, textbooks      216
Ligands      99 100 101 102 155 159 245 see ligand
Lineweaver — Burk equation      281—301
Lipids, analysis      64 70 88 303—319 371—387
Lipids, extraction from nutmeg      303—319
Lipids, extraction from plants      333—343
Lipids, gas chromatography      69 308 315
Lipids, purification      303—319
Lipids, structure      304
Lipids, thin-layer chromatography      305—319
Liquid scintillation counting      176—181
Lowry protein assay      41 42 43
Lutein      334 335 337
Lyophilization (freeze-drymg)      52—54
Lysosomes      202 206
Lysozyme      259 403
Malate dehydrogenase      361 366—367
Mass spectrometry      167—168
Material Safety Data Sheet (MSDS)      4 5 7
Mean      see Statistical analysis
Membrane filtration      see Ultrafiltration
Membranes, blotting      136 322
Membranes, mitochondrial      357—361
Membranes, proton transport      345—355
Mercaptoethanol      119 230 262 434
Michaelis constant (KM)      281—283 372 392
Michaelis-Menten equation      246 280—283 372
Microcentrifuges      see Centrifuges
Microsomes      202 206 262 359
Milk proteins in Western blotting      323
Milk proteins, extraction of      257—277
Mitochondria, enzyme profile      357—370
Mitochondria, isolation and characterization      201—202 206 262 357—370
Mitochondria, structure and function      357—362
Mitochondria, subfractionation      360 365—366
Molanty      11 12
Molar absorption coefficient (E)      145 296—297 see
Molecular weight, determination by centnfugation      204
Molecular weight, determination by electrophoresis      119—121 124 138 257—277
Molecular weight, determination by gel filtration      85—87
Molecular weight, determination by ms      168
Molecular weight, table of proteins      446
Monoamine oxidase      361 367
N,N'-methylene bisacrylamide      113—115 133 268 272—273 391
N,N,N',N'-tetramethylethylenedi-amine (TEMED)      113—114 133 268 272—273 393
NAD/NADH      see Nicotinamide adenine dinucleotide
Neoxanthin      334 335 337
Nicotinamide adenine dinucleotide      154 346—347 361 368
Ninhydnn      64 229 238
Northern blotting      136—137 329
Notebook, laboratory      8—10
Nuclear magnetic resonance spectroscopy      163—167
Nuclei, cell      202 262 415
Nucleic acids      see also Deoxynbonucleic acid; Ribonucleic acid
Nucleic acids in protein assay      45
Nucleic acids, agarose gel electrophoresis      122—127 415—429 431—442
Nucleic acids, analysis      46—48 88 204
Nucleic acids, blotting      136—138
Nucleic acids, centrifugation      206

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