Discusses principles, techniques, and instrumentation involving HPLC, delineating its usage in separation, purification, and detection processes across a wide variety of disciplines in industry and research. Part I covers HPLC theory, and Part II discusses different HPLC modes and critically addresses their applicability and limitations. Part III describes principles and practical operations of modern HPLC equipment, and Part IV examines applications. For chemists, biologists, and students.