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Gosling J.P. (ed.) — Immunoassays: A Practical Approach
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Название: Immunoassays: A Practical ApproachАвтор: Gosling J.P. (ed.) Аннотация: A practical manual presenting step-by-step protocols designed to help biologists develop an immunoassay in any common format for any suitable analyte. Describes methods for raising and choosing polyclonal and monoclonal antibodies, immunogen preparation and characterization, sourcing standards, the synthesis and characterization of radioactive, enzymatic, fluorescent, and ligand labels, the preparation of a wide variety of solid-phase reagents, assay optimization, validation of quantitative and qualitative assays, curve fitting and data processing, and quality assurance procedures for small- and large-scale users.
Язык: Рубрика: Технология /Статус предметного указателя: Готов указатель с номерами страниц ed2k: ed2k stats Год издания: 2000Количество страниц: 303Добавлена в каталог: 23.11.2006Операции: Положить на полку |
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Предметный указатель
"Abraham method" 215-216 "Two-step" assay 208 90 93 97 98 4-MU-Gal 98 4-MUP 98 4PL model 245-247 260 ABDP 105 ABTS 95 Accuracy 196-208 224-230 235 Accuracy, diagnostic 231-232 235 Accuracy, factors affecting 196-197 Active ester procedure 106 107 119-120 Adjuvants 26-29 Adsorption methods 140-141 155-156 Affinity 62-67 Affinity and sensitivity 76 Affinity, chromatography 52-53 54-55 Affinity, constant 62-63 Affinity, constant, calculation 63-66 82-83 Affinity, estimation 76-85 Affinity, estimation, by ELISA 80-83 84 Affinity, estimation, by equilibrium dialysis 77-80 Affinity, estimation, by surface plasmon resonance 83 85 Affinity, factors affecting 66-67 Affinity, functional 62 Affinity, intrinsic 62 Affinity, maturation 4 26 Agar, cloning on 45 46 Agglutination 8 9 Aldehydes 138 157 158-159 Alkaline phosphatase 90 93 97 98 99 126 All-laboratory consensus means 286 292 All-method performance 287 Alum adjuvant 27 28-29 Ambient analyte assays 8 13 Amino group to amino group conjugation 104-105 135 174-176 Amino group to carbohydrate side-chain conjugation 108-109 Amino group to carboxyl group conjugation 105-106 135 172-174 Amino group to sulfhydryl group conjugation 106 108 135 138 177-178 Ammonium Sulfate Precipitation 52 53 Analyte concentration 230-231 Analyte, notation system for 7 Analytical goals 212 Analytical recovery 224-226 Analytical run 266 Anhydride conjugation 106 118-119 135 148-149 174 Animals, antibody raising 21-25 Animals, bleeds 23 Animals, immunogen dose 26 Animals, welfare 167-168 Anti-Ig antibody interference 203-208 Anti-Ig antibody interference, negative 204 205 Anti-Ig antibody interference, positive 204 Anti-Ig secondary antibodies 60 Anti-nucleic acid antibodies 26 Anti-species Ig antibodies 59-60 Antibiotic supplements 39 Antibodies 19 Antibodies, advantages of 1 Antibodies, purification 51-55 Antibodies, raising 19-58 Antibodies, secondary 59-61 94 Antibodies, selection 20-21 59-86 205-207 Antibodies, sources 67-69 Antibody capture 33-34 35-36 Antibody fragments 4-5 142 149 208 Antibody screening 32-36 Antibody-antigen saturation curve 82 Antifungals 39 Antigen capture 34 36 Antigen-antibody binding 5-7 Antigen-antibody binding, binding reaction 6-7 Ascites tumours 49-50 Assay see "Immunoassay" Association 6 Audit 289 Automated assays 7 130 Automated assays, quality control 280-281 Avidin 94 Avidity 62 Azaserine 37-38 Baseline assay 271-272 BCIG 97 BCIP/NBT 97 Beads see "Microspheres" Between-laboratory agreement 287 BIAcore 83 85 Bias 265 283 288 Bias, curve fitting 248-249 Binding reaction 6-7 Binding, nonspecific 142 Binding, specificity 61 Biological validation 230-232 Biotin 90 94 95 122-123 Blank specimens 271 bleeds 23 Blocking agents/procedures 142-144 145 146-147 Bovine serum albumin (BSA) 171 Calibration curves see "Curve-fitting" Calibrator preparation 196-197 267 Carbodiimides 106 135 151-152 172-173 Carbohydrate side-chain conjugation 108-109 Carbonyldiimidazole (CDI) 135 173-174 Carboxyl conjugation 105-106 135 172-174 Carriers 171 Casein 142 143 CDI (carbonyldiimidazole) 135 173-174 Cell culture equipment 37 Cell culture media 37 Cell culture media, preparation 37 38 Cell culture media, recipes 37 Cell culture media, supplements 37-41 Cell culture techniques 50-51 Cell lines 21 295 Cell storage 47-49 Cell viability 41 42-43 Certified reference materials 270 Chemiluminescence 89 98-99 Chicken, antibodies 24-25 Chicken, bleeds 23 Chicken, immunogen dose 26 Chloronaphthol 96 Chromatography, affinity 52-53 54-55 Chromatography, hydroxyapatite 52 54 Chromogens 95-97 classification system 8-13 Clinical validation 230-232 Cloning, by limiting dilution 45-47 Cloning, on soft agar 45 46 Coating methods 140 145 146-147 Collisions 6-7 Column effect 198 Competitive assays 13 Competitive assays, antibodies for 70-72 Competitive assays, crossreactivity study 71 72 215-216 Competitive assays, optimization 191-193 Complementarity-determining regions 2-3 Computerized curve fitting 245-247 260 Concentrations 230-231 Conjugate stability 125-126 Conjugation 26 104-123 132-140 170-182 Conjugation ratio 124-125 180-182 Conjugation, active ester 106 107 119-120 Conjugation, aldehyde 138 157 158-159 Conjugation, anhydride 106 118-119 135 148-149 174 Conjugation, carbodiimide 106 135 151-152 172-173 Conjugation, carbonyldiimidazole (CDI) 135 173-174 Conjugation, epoxide 135 Conjugation, glutaraldehyde 104 109-111 176 Conjugation, hetero-bifunctional reagents 104-105 106 108 118 120-121 178 179 Conjugation, hydrazide 138 Conjugation, maleimide 138 149-150 177 Conjugation, Mannich condensation 178 179 Conjugation, NHS 134-135 175 Conjugation, periodate oxidation 108-109 111-112 Conjugation, tosylate 135 157 158 Consensus means 286 292 Correlation studies 228-230 Coupling see also "Conjugation" Coupling, oriented 138-139 Coupling, random 139-140 Covalent coupling 132-140 156-159 Crosslinking reagents 171-172 Crossreactants 14 Crossreactants, interference by 199-200 Crossreactivity evaluation 71 72 199 213-217 Culture equipment 37 Culture media 37 Culture media, preparation 37 38 Culture media, recipes 37 Culture media, supplements 37-41 Culture techniques 50-51 Cumulative sum chart 281 Curve-fitting 239-261 Curve-fitting, bias 248-249 Curve-fitting, computerized 245-247 260 Curve-fitting, empirical 244 Curve-fitting, four parameter log-logistic model (4PL) 245-247 260 Curve-fitting, logit-log model 244 245 247 254-259 Curve-fitting, manual 241-244 Curve-fitting, method selection 247-253 Curve-fitting, theoretical 244-245 Curve-fitting, weighted 248 DAB 96 data sheets 67 68 DELFIA 121-122 Denaturation 160 Detergents 143-144 Diagnostic accuracy 231-232 235 Diagnostic sensitivity 235 Diagnostic specificity 235 Dioxetanes 99 Dithiothreitol 207 DMA 175-176 DMP 175-176 DMS 175-176 Dose metameters 240 Dose-response curves 240 Dot-blot ELISA 151 153-154 DPEM 105 Drift 218 219 DTBP 175-176 EDAC 172 Edge effects 144-145 198-199 218 ELISA 14 ELISA, affinity estimation 80-83 84 ELISA, immunodot-blot 151 153-154 ELISA, labelled-antigen 75-76 Endpoint measurement 90 Enthalpy 194 entropy 194 Enzyme conjugate stability 125-126 Enzyme labels 90 92-93 Enzyme labels, storage 125-126 Enzyme measurement 95-99 Enzyme measurement, with chemiluminescence 98-99 Enzyme measurement, with chromogens 95-97 Enzyme measurement, with fluorogens 97-98 Enzyme-hapten coupling 117-121 Epitopes 6 Epitopes, density 66 Epitopes, mapping 61-62 Epoxides 135 Equilibrium dialysis 77-80 Equipment 37 Equipment, maintenance 266-267 Errors 265 283 External quality assessment 198 282-292 External quality assessment, limitations of 289 292 External quality assessment, providers 296-297 External quality assessment, specimens 271 285 F(ab')2 preparation 113-114 FAB 4 5 Fab fragments 4 5 142 149 208 Fab' 4 5 Fab', preparation 115-116 Fab'-enzyme conjugates 112-117 Fc fragments 4 5 Feeder cell preparation 39-40 Fetal calf serum (FCS) 37 Fluorescein 93 94 95 122 Fluorescent labels 90 93-94 121-122 Fluorogens 97-98 Four parameter log-logistic model (4PL) 245-247 260 Freezing cells 47-49 Freund’s adjuvant 27-28 Fusion procedures 41 Fv fragments 4 5 Gel filtration 52 Gelatin 142 Glutamine 37 Glutaraldehyde coupling 104 109-111 176 Goat 26 Grey zone 233-234 Guinea pig 23 Haloacetyls 177 Hamster 23 Hapten 5-6 Hapten-carriers 166-167 Hapten-carriers, conjugation 170-182 Hapten-carriers, conjugation, anhydride 174 Hapten-carriers, conjugation, carbodiimide 172-173 Hapten-carriers, conjugation, carbonyldiimazole 173-174 Hapten-carriers, conjugation, glutaraldehyde 176 Hapten-carriers, conjugation, hetero-bifunctional reagents 178 179 Hapten-carriers, conjugation, imidoesters 175-176 Hapten-carriers, conjugation, Mannich condensation 178 179 Hapten-carriers, conjugation, NHS 175 Hapten-carriers, conjugation, sulfhydryl reactive groups 177-178 Hapten-enzyme coupling 117-121 HAT sensitivity 37-38 Hetero-bifunctional reagents 104-105 106 108 118 120-121 178 179 Heterophilic antibodies 203 Heteroscedasticity 240 High-dose hook effect 194 Hollow-fibre cell culture 51 Homogeneous assays 8 13 129 Homoscedasticity 240 Horseradish peroxidase (HRP) 90 92-93 95-96 98 99 126 Horseradish peroxidase (HRP), activation with SMCC 114-115 Horseradish peroxidase (HRP), conjugation to progesterone 118-119 HPAA 98 HPPA 98 HSAB 105 Hybridoma cell production 37-51 Hybridoma cell production, cell freezing 47-49 Hybridoma cell production, cloning 45-47 Hybridoma cell production, equipment 37 Hybridoma cell production, fusion procedure 41 43-45 Hybridoma cell production, media 37-41 Hydrazide 138 Hydrocoatings 141-142 Hydromorphine 199 Hydroxyapatite chromatography 52 54 Hydroxyl conjugation 135 Imidoesters 175-176 Immobilization methods 131-144 Immobilization methods, covalent attachment 132-140 156-159 Immobilization methods, indirect 141-142 Immobilization methods, ligand preparation 132
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