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Gosling J.P. (ed.) — Immunoassays: A Practical Approach
Gosling J.P. (ed.) — Immunoassays: A Practical Approach

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Название: Immunoassays: A Practical Approach

Автор: Gosling J.P. (ed.)

Аннотация:

A practical manual presenting step-by-step protocols designed to help biologists develop an immunoassay in any common format for any suitable analyte. Describes methods for raising and choosing polyclonal and monoclonal antibodies, immunogen preparation and characterization, sourcing standards, the synthesis and characterization of radioactive, enzymatic, fluorescent, and ligand labels, the preparation of a wide variety of solid-phase reagents, assay optimization, validation of quantitative and qualitative assays, curve fitting and data processing, and quality assurance procedures for small- and large-scale users.


Язык: en

Рубрика: Технология/

Статус предметного указателя: Готов указатель с номерами страниц

ed2k: ed2k stats

Год издания: 2000

Количество страниц: 303

Добавлена в каталог: 23.11.2006

Операции: Положить на полку | Скопировать ссылку для форума | Скопировать ID
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Предметный указатель
"Abraham method"      215-216
"Two-step" assay      208
$\beta$-Galactosidase      90 93 97 98
4-MU-Gal      98
4-MUP      98
4PL model      245-247 260
ABDP      105
ABTS      95
Accuracy      196-208 224-230 235
Accuracy, diagnostic      231-232 235
Accuracy, factors affecting      196-197
Active ester procedure      106 107 119-120
Adjuvants      26-29
Adsorption methods      140-141 155-156
Affinity      62-67
Affinity and sensitivity      76
Affinity, chromatography      52-53 54-55
Affinity, constant      62-63
Affinity, constant, calculation      63-66 82-83
Affinity, estimation      76-85
Affinity, estimation, by ELISA      80-83 84
Affinity, estimation, by equilibrium dialysis      77-80
Affinity, estimation, by surface plasmon resonance      83 85
Affinity, factors affecting      66-67
Affinity, functional      62
Affinity, intrinsic      62
Affinity, maturation      4 26
Agar, cloning on      45 46
Agglutination      8 9
Aldehydes      138 157 158-159
Alkaline phosphatase      90 93 97 98 99 126
All-laboratory consensus means      286 292
All-method performance      287
Alum adjuvant      27 28-29
Ambient analyte assays      8 13
Amino group to amino group conjugation      104-105 135 174-176
Amino group to carbohydrate side-chain conjugation      108-109
Amino group to carboxyl group conjugation      105-106 135 172-174
Amino group to sulfhydryl group conjugation      106 108 135 138 177-178
Ammonium Sulfate Precipitation      52 53
Analyte concentration      230-231
Analyte, notation system for      7
Analytical goals      212
Analytical recovery      224-226
Analytical run      266
Anhydride conjugation      106 118-119 135 148-149 174
Animals, antibody raising      21-25
Animals, bleeds      23
Animals, immunogen dose      26
Animals, welfare      167-168
Anti-Ig antibody interference      203-208
Anti-Ig antibody interference, negative      204 205
Anti-Ig antibody interference, positive      204
Anti-Ig secondary antibodies      60
Anti-nucleic acid antibodies      26
Anti-species Ig antibodies      59-60
Antibiotic supplements      39
Antibodies      19
Antibodies, advantages of      1
Antibodies, purification      51-55
Antibodies, raising      19-58
Antibodies, secondary      59-61 94
Antibodies, selection      20-21 59-86 205-207
Antibodies, sources      67-69
Antibody capture      33-34 35-36
Antibody fragments      4-5 142 149 208
Antibody screening      32-36
Antibody-antigen saturation curve      82
Antifungals      39
Antigen capture      34 36
Antigen-antibody binding      5-7
Antigen-antibody binding, binding reaction      6-7
Ascites tumours      49-50
Assay      see "Immunoassay"
Association      6
Audit      289
Automated assays      7 130
Automated assays, quality control      280-281
Avidin      94
Avidity      62
Azaserine      37-38
Baseline assay      271-272
BCIG      97
BCIP/NBT      97
Beads      see "Microspheres"
Between-laboratory agreement      287
BIAcore      83 85
Bias      265 283 288
Bias, curve fitting      248-249
Binding reaction      6-7
Binding, nonspecific      142
Binding, specificity      61
Biological validation      230-232
Biotin      90 94 95 122-123
Blank specimens      271
bleeds      23
Blocking agents/procedures      142-144 145 146-147
Bovine serum albumin (BSA)      171
Calibration curves      see "Curve-fitting"
Calibrator preparation      196-197 267
Carbodiimides      106 135 151-152 172-173
Carbohydrate side-chain conjugation      108-109
Carbonyldiimidazole (CDI)      135 173-174
Carboxyl conjugation      105-106 135 172-174
Carriers      171
Casein      142 143
CDI (carbonyldiimidazole)      135 173-174
Cell culture equipment      37
Cell culture media      37
Cell culture media, preparation      37 38
Cell culture media, recipes      37
Cell culture media, supplements      37-41
Cell culture techniques      50-51
Cell lines      21 295
Cell storage      47-49
Cell viability      41 42-43
Certified reference materials      270
Chemiluminescence      89 98-99
Chicken, antibodies      24-25
Chicken, bleeds      23
Chicken, immunogen dose      26
Chloronaphthol      96
Chromatography, affinity      52-53 54-55
Chromatography, hydroxyapatite      52 54
Chromogens      95-97
classification system      8-13
Clinical validation      230-232
Cloning, by limiting dilution      45-47
Cloning, on soft agar      45 46
Coating methods      140 145 146-147
Collisions      6-7
Column effect      198
Competitive assays      13
Competitive assays, antibodies for      70-72
Competitive assays, crossreactivity study      71 72 215-216
Competitive assays, optimization      191-193
Complementarity-determining regions      2-3
Computerized curve fitting      245-247 260
Concentrations      230-231
Conjugate stability      125-126
Conjugation      26 104-123 132-140 170-182
Conjugation ratio      124-125 180-182
Conjugation, active ester      106 107 119-120
Conjugation, aldehyde      138 157 158-159
Conjugation, anhydride      106 118-119 135 148-149 174
Conjugation, carbodiimide      106 135 151-152 172-173
Conjugation, carbonyldiimidazole (CDI)      135 173-174
Conjugation, epoxide      135
Conjugation, glutaraldehyde      104 109-111 176
Conjugation, hetero-bifunctional reagents      104-105 106 108 118 120-121 178 179
Conjugation, hydrazide      138
Conjugation, maleimide      138 149-150 177
Conjugation, Mannich condensation      178 179
Conjugation, NHS      134-135 175
Conjugation, periodate oxidation      108-109 111-112
Conjugation, tosylate      135 157 158
Consensus means      286 292
Correlation studies      228-230
Coupling      see also "Conjugation"
Coupling, oriented      138-139
Coupling, random      139-140
Covalent coupling      132-140 156-159
Crosslinking reagents      171-172
Crossreactants      14
Crossreactants, interference by      199-200
Crossreactivity evaluation      71 72 199 213-217
Culture equipment      37
Culture media      37
Culture media, preparation      37 38
Culture media, recipes      37
Culture media, supplements      37-41
Culture techniques      50-51
Cumulative sum chart      281
Curve-fitting      239-261
Curve-fitting, bias      248-249
Curve-fitting, computerized      245-247 260
Curve-fitting, empirical      244
Curve-fitting, four parameter log-logistic model (4PL)      245-247 260
Curve-fitting, logit-log model      244 245 247 254-259
Curve-fitting, manual      241-244
Curve-fitting, method selection      247-253
Curve-fitting, theoretical      244-245
Curve-fitting, weighted      248
DAB      96
data sheets      67 68
DELFIA      121-122
Denaturation      160
Detergents      143-144
Diagnostic accuracy      231-232 235
Diagnostic sensitivity      235
Diagnostic specificity      235
Dioxetanes      99
Dithiothreitol      207
DMA      175-176
DMP      175-176
DMS      175-176
Dose metameters      240
Dose-response curves      240
Dot-blot ELISA      151 153-154
DPEM      105
Drift      218 219
DTBP      175-176
EDAC      172
Edge effects      144-145 198-199 218
ELISA      14
ELISA, affinity estimation      80-83 84
ELISA, immunodot-blot      151 153-154
ELISA, labelled-antigen      75-76
Endpoint measurement      90
Enthalpy      194
entropy      194
Enzyme conjugate stability      125-126
Enzyme labels      90 92-93
Enzyme labels, storage      125-126
Enzyme measurement      95-99
Enzyme measurement, with chemiluminescence      98-99
Enzyme measurement, with chromogens      95-97
Enzyme measurement, with fluorogens      97-98
Enzyme-hapten coupling      117-121
Epitopes      6
Epitopes, density      66
Epitopes, mapping      61-62
Epoxides      135
Equilibrium dialysis      77-80
Equipment      37
Equipment, maintenance      266-267
Errors      265 283
External quality assessment      198 282-292
External quality assessment, limitations of      289 292
External quality assessment, providers      296-297
External quality assessment, specimens      271 285
F(ab')2 preparation      113-114
FAB      4 5
Fab fragments      4 5 142 149 208
Fab'      4 5
Fab', preparation      115-116
Fab'-enzyme conjugates      112-117
Fc fragments      4 5
Feeder cell preparation      39-40
Fetal calf serum (FCS)      37
Fluorescein      93 94 95 122
Fluorescent labels      90 93-94 121-122
Fluorogens      97-98
Four parameter log-logistic model (4PL)      245-247 260
Freezing cells      47-49
Freund’s adjuvant      27-28
Fusion procedures      41
Fv fragments      4 5
Gel filtration      52
Gelatin      142
Glutamine      37
Glutaraldehyde coupling      104 109-111 176
Goat      26
Grey zone      233-234
Guinea pig      23
Haloacetyls      177
Hamster      23
Hapten      5-6
Hapten-carriers      166-167
Hapten-carriers, conjugation      170-182
Hapten-carriers, conjugation, anhydride      174
Hapten-carriers, conjugation, carbodiimide      172-173
Hapten-carriers, conjugation, carbonyldiimazole      173-174
Hapten-carriers, conjugation, glutaraldehyde      176
Hapten-carriers, conjugation, hetero-bifunctional reagents      178 179
Hapten-carriers, conjugation, imidoesters      175-176
Hapten-carriers, conjugation, Mannich condensation      178 179
Hapten-carriers, conjugation, NHS      175
Hapten-carriers, conjugation, sulfhydryl reactive groups      177-178
Hapten-enzyme coupling      117-121
HAT sensitivity      37-38
Hetero-bifunctional reagents      104-105 106 108 118 120-121 178 179
Heterophilic antibodies      203
Heteroscedasticity      240
High-dose hook effect      194
Hollow-fibre cell culture      51
Homogeneous assays      8 13 129
Homoscedasticity      240
Horseradish peroxidase (HRP)      90 92-93 95-96 98 99 126
Horseradish peroxidase (HRP), activation with SMCC      114-115
Horseradish peroxidase (HRP), conjugation to progesterone      118-119
HPAA      98
HPPA      98
HSAB      105
Hybridoma cell production      37-51
Hybridoma cell production, cell freezing      47-49
Hybridoma cell production, cloning      45-47
Hybridoma cell production, equipment      37
Hybridoma cell production, fusion procedure      41 43-45
Hybridoma cell production, media      37-41
Hydrazide      138
Hydrocoatings      141-142
Hydromorphine      199
Hydroxyapatite chromatography      52 54
Hydroxyl conjugation      135
Imidoesters      175-176
Immobilization methods      131-144
Immobilization methods, covalent attachment      132-140 156-159
Immobilization methods, indirect      141-142
Immobilization methods, ligand preparation      132
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