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Gosling J.P. (ed.) — Immunoassays: A Practical Approach
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Название: Immunoassays: A Practical ApproachАвтор: Gosling J.P. (ed.) Аннотация: A practical manual presenting step-by-step protocols designed to help biologists develop an immunoassay in any common format for any suitable analyte. Describes methods for raising and choosing polyclonal and monoclonal antibodies, immunogen preparation and characterization, sourcing standards, the synthesis and characterization of radioactive, enzymatic, fluorescent, and ligand labels, the preparation of a wide variety of solid-phase reagents, assay optimization, validation of quantitative and qualitative assays, curve fitting and data processing, and quality assurance procedures for small- and large-scale users.
Язык: Рубрика: Технология /Статус предметного указателя: Готов указатель с номерами страниц ed2k: ed2k stats Год издания: 2000Количество страниц: 303Добавлена в каталог: 23.11.2006Операции: Положить на полку |
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Предметный указатель
Immobilization methods, noncovalent adsorption 140-141 155-156 Immunization 25-32 Immunization, in vitro 55 Immunization, injections 29 Immunization, monitoring 32-36 Immunoassay 1-2 Immunoassay, accuracy see "Accuracy" Immunoassay, automated 7 130 Immunoassay, automated, quality control 280-281 Immunoassay, designs 8-13 130 Immunoassay, drift 218 219 Immunoassay, formats 14 Immunoassay, interference 197 199-208 Immunoassay, kits 295-296 Immunoassay, nomenclature 13-14 Immunoassay, notation system 7-8 Immunoassay, optimization 187-210 Immunoassay, precision see "Precision" Immunoassay, sensitivity see "Sensitivity" Immunoassay, specificity see "S\pecificity" Immunoassay, validation 211-238 Immunodot-blot ELISA 151 153-154 Immunogens 25-26 165-182 Immunogens, characterization 180-182 Immunogens, dose 26 Immunogens, purification 168-169 Immunogens, recombinant 170 Immunogens, storage 182 Immunogens, suppliers 295 Immunogens, synthetic 169-170 Immunoglobulin G (IgG) 2-4 Immunoglobulin M (IgM) 4 Immunometric assay 14 Immunoreactivity 123-124 Immunosensors 8 9 Imprecision see "Precision" In vitro immunization 55 Incubation time 195 196 Injection routes 29 Interference 197 199-208 Internal quality control 198 265-282 283 Internal quality control, automated assays 280-281 Internal quality control, benefits of 277 Internal quality control, charts 272-273 281 Internal quality control, implementing 276-277 Internal quality control, limitations of 278 280 Internal quality control, repeat analytical controls 281 Internal quality control, results interpretation 273-274 Internal quality control, rules 274-276 Internal quality control, specimens 268-271 Iodine-125 89 90 Iodine-125, radioiodination 99-103 Iodogen 102-103 Isobutylchloroformate method 174 Label-free assays 8-9 Labelled-antigen ELISA 75-76 labels 66-67 89-95 Labels, chemiluminescent 89 Labels, enzymatic 90 92-93 125-126 Labels, fluorescent 90 93-94 121-122 Labels, immunoreactivity 123-124 Labels, indirect 94-95 Labels, ligand 90 94 Labels, luminescent 90 Labels, microparticle 90 Labels, radioisotopic 89-92 99-103 Labels, secondary 94 Labels, specific activity 124-125 Labels, stability 125 Labels, storage 125-126 Labels, suppliers 296 Labels, vesicle 90 Laboratory performance 288-289 Laboratory reports 265 Lactoperoxidase 103 Langmuir plot 64-65 Lanthanide chelates 93-94 121-122 Latex microspheres 90 130-131 155-160 297-298 Latex microspheres, aldehyde-modified 157 158-159 Latex microspheres, amino-modified 156-157 Latex microspheres, bead size 155 Latex microspheres, carboxyl-modified 156-157 Latex microspheres, storage 161 Latex microspheres, sulfated 155 Latex microspheres, tosyl-activated 157 158 Levey — Jennings chart 272 273 Ligand immobilization see "Immobilization methods" Ligand labels 90 94 Limit of quantification 190-191 Limiting dilution cloning 45-47 Linearizing transformations 241 Linscotts Directory 68 Liquid phase assay 130 Log-logistic model (4PL) 245-247 260 Logit-log model 244 245 247 254-259 Lower limit of detection 188-190 Luminescence 90 98-99 Lyophilized sera 270 Maleic anhydride plates 135 148-149 Maleimides 177 Maleimides, measurement 115 Maleimides, plates 138 149-150 Mannich condensation 178 179 Maps 170 MBS 105 Media, preparation 37 38 Media, recipes 37 Media, supplements 37-41 Membranes 150-154 298 Membranes, carbodiimide-activated 151-152 Membranes, handling 161 Membranes, storage 161 Method means 286 Method performance 287-288 Mice see "Mouse" Microarray/microchip 8 13 Microparticle labels 90 Microspheres 90 130-131 155-160 297-298 Microspheres, aldehyde-modified 157 158-159 Microspheres, amino-modified 156-157 Microspheres, bead size 155 Microspheres, carboxyl-modified 156-157 Microspheres, storage 161 Microspheres, sulfated 155 Microspheres, tosyl-activated 157 158 Microtitre plates 130 144-150 297 Microtitre plates, activated 145-146 148-149 Microtitre plates, binding abilities 144 Microtitre plates, blocking 142-144 145 146-147 Microtitre plates, coating 140 145 146-147 Microtitre plates, column effect 198 Microtitre plates, commercially prepared 145 147-149 Microtitre plates, edge effects 144-145 198-199 218 Microtitre plates, handling 160-161 Microtitre plates, storage 145-146 160 Mitsunobu reaction 138 Monoclonal antibodies 19 20 Monoclonal antibodies, raising 21-23 30-31 33 49-51 Morphine 199 Mouse, bleeds 23 Mouse, immunization 29 30-31 Mouse, immunogen dose 26 Mouse, myeloma cell lines 21 MultiCalc™ 260 Myeloma cell lines 21-22 NHS coupling 134-135 175 Nomenclature 13-14 Noncompetitive assay optimization 193-194 Noncovalent adsorption 140-141 155-156 Nonspecific binding 142 Notation system 7-8 Nucleic acid antigens 26 Nylon membranes see "Membranes" One-site assays 8 9 ONPG 97 OPD 96 Oriented coupling 138-139 Ouchterlony method 62 73 74 Oxiranes 135 Paratopes 2-3 5-6 Particle capture assay 130 Performance assessment 286-289 Periodate oxidation 108-109 111-112 Ph 7 195 Phage display 56 Plant antibodies 56 Plasma binding proteins 200-203 Plasmid DNA expression vectors 26 PNPP 97 Polyclonal antibodies 19 20 Polyclonal antibodies, raising 23-25 32-33 Polymer blocking agents 143-144 Polyspecific antibodies 203 Polystyrene microspheres 155 156 Polystyrene tubes 150 Pooled material 269-270 Positional effects 144-145 198-199 218 Precipitation 8 9 PRECISION 217-222 265 283 288 Precision, profiles 223 Product information sheets 67 68 Protease inhibitors 182 Protein assays 183 Protein blocking agents 142-143 Protein denaturation 160 Protein G affinity chromatography 52-53 54-55 Protein-protein conjugation 104-105 109-117 Protein-protein conjugation, glutaraldehyde method 109-111 Protein-protein conjugation, periodate oxidation 111-112 Purification methods 168-169 Purification methods, affinity chromatography 52-53 54-55 Purification methods, ammonium sulfate precipitation 52 53 Purification methods, hydroxyapatite chromatography 52 54 Pyridyl disulphides 177 Qualitative assays 232 Qualitative assays, accuracy 235 Qualitative assays, grey zone 233-234 Qualitative assays, specificity 232-233 Qualitative assays, validation 233-235 Quality assessment 198 Quality assessment, limitations of 289 292 Quality assessment, providers 296-297 Quality assessment, specimens 271 285 Quality assurance 263-293 296-297 Quality control 198 265-282 283 Quality control of automated assays 280-281 Quality control, benefits of 277 Quality control, charts 272-273 281 Quality control, implementing 276-277 Quality control, limitations of 278 280 Quality control, repeat analytical controls 281 Quality control, results interpretation 273-274 Quality control, rules 274-276 Quality control, specimens 268-271 Rabbit, antibodies 23 Rabbit, bleeds 23 Rabbit, immunization 29 30 Rabbit, immunogen dose 26 Radioiodination 99-103 Radioiodination of immunoglobulin with iodogen 102-103 Radioiodination of immunoglobulin with lactoperoxidase 103 Radioiodination, safety 100-101 Radioisotopic labels 89-92 99-103 Radioreceptor assay 14 Random coupling 139-140 Random error 265 Rat, antibodies 22-23 Rat, bleeds 23 Rat, immunization 29 Rat, immunogen dose 26 Rat, myeloma cell lines 21-22 Reagent excess assays 8 9 13 Reagent limited assays 8 13 see Reagents, concentration optimization 191-194 Reagents, sourcing 161 Reagents, stability 235-237 Reagents, storage 236 267 Recombinant antibodies 55-56 Recombinant immunogens 170 Recombinant immunogens, recovery of added standard 224-226 Recovery specimens 271 Reducing agents 207 Reference method 228-230 266 286 Repeat analytical controls 281 Response metameters 240 Response-error relationship 240 Results, reporting 265 Rheumatoid factors 203 Run 266 SAMSA 104-105 Sandwich assay 9 SATA 105 Scatchard plot 63-64 Screening procedures 32-36 Secondary antibodies 59-61 94 Secondary labels 94 Semiquantitative assay 232 Semiquantitative assay, accuracy 235 Semiquantitative assay, grey zone 233-234 Semiquantitative assay, specificity 232-233 Semiquantitative assay, validation 233-235 Sensitivity 76 187-196 222-224 Sensitivity and incubation time 195 196 Sensitivity and pH 195 Sensitivity and temperature 194 195 196 Sensitivity, and reagent concentration 191-194 Sensitivity, defined 188 Sensitivity, diagnostic 235 Sensitivity, estimators 187-191 223-224 Sensitivity, estimators, limit of quantification 190-191 Sensitivity, estimators, lower limit of detection 189-190 223-224 Separation assay see "Solid-phase immunoassay" Separation free assay 8 13 129 Serum preparation 32 SIAB 108 Single-chain variable-fragment phage display libraries 56 Sips plot 65-66 SMCC 105 106 114-115 Software 260 Solid-phase immunoassay 129-163 Solid-phase immunoassay, immobilization methods 131-144 Solid-phase immunoassay, immobilization methods, covalent attachment 132-140 156-159 Solid-phase immunoassay, immobilization methods, indirect 141-142 Solid-phase immunoassay, immobilization methods, ligand preparation 132 Solid-phase immunoassay, immobilization methods, noncovalent adsorption 140-141 155-156 Solid-phase immunoassay, membranes 150-154 298 Solid-phase immunoassay, microspheres 130-131 155-160 297-298 Solid-phase immunoassay, microtitre plates 130 144-150 297 Solid-phase immunoassay, supports 130-131 Solid-phase immunoassay, tubes 150 298 Spacer arms/groups 117 SPDP 105 Specific activity 124 Specificity 14-15 61 69-76 212-217 232-233 Specificity, diagnostic 235 Specimens 264-265 Specimens for quality assurance 268-271 285 Spleen lymphokine 39 40-41 Splenocyte feeder cells 39-40 Stability of reagents 235-237
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