√лавна€    Ex Libris     ниги    ∆урналы    —татьи    —ерии     аталог    Wanted    «агрузка    ’удЋит    —правка    ѕоиск по индексам    ѕоиск    ‘орум   
blank
јвторизаци€

       
blank
ѕоиск по указател€м

blank
blank
blank
 расота
blank
Budowle B. Ч DNA Typing Protocols
Budowle B. Ч DNA Typing Protocols



ќбсудите книгу на научном форуме



Ќашли опечатку?
¬ыделите ее мышкой и нажмите Ctrl+Enter


Ќазвание: DNA Typing Protocols

јвтор: Budowle B.

јннотаци€:

(BioTechniques Books) FBI, Washington, DC. Laboratory manual provides a comprehensive compendium of DNA typing techniques. Compiled by a team of prominent biomolecular scientists from the FBI Forensic Sciences Research and Training Laboratory. Topics include DNA extraction techniques. Outline format. Plastic-comb binding.


язык: en

–убрика: “ехнологи€/

—татус предметного указател€: √отов указатель с номерами страниц

ed2k: ed2k stats

√од издани€: 2000

 оличество страниц: 304

ƒобавлена в каталог: 21.11.2006

ќперации: ѕоложить на полку | —копировать ссылку дл€ форума | —копировать ID
blank
ѕредметный указатель
$^{32}P$-labeled alphoid probe, slot blot hybridization, DNA quantity determination      50Ч53
ABI PRISM 310 Genetic Analyzer      159 167f 184f
ABI PRISM 310 Genetic Analyzer, buffer and waste vials      162
ABI PRISM 310 Genetic Analyzer, capillary      162
ABI PRISM 310 Genetic Analyzer, electrode installation      168 168f
ABI PRISM 310 Genetic Analyzer, honing      167Ч168
ABI PRISM 310 Genetic Analyzer, matrix      162
ABI PRISM 310 Genetic Analyzer, response evaluation      162
ABI PRISM 310 Genetic Analyzer, setting up run      169Ч176
ABI PRISM 310 Genetic Analyzer, setting up run, autosampler calibration      171
ABI PRISM 310 Genetic Analyzer, setting up run, buffer reservoir filling      171Ч172
ABI PRISM 310 Genetic Analyzer, setting up run, capillary installation      170Ч171
ABI PRISM 310 Genetic Analyzer, setting up run, priming      172
ABI PRISM 310 Genetic Analyzer, setting up run, pump block      170
ABI PRISM 310 Genetic Analyzer, setting up run, sample preparation      172
ABI PRISM 310 Genetic Analyzer, setting up run, syringe      169Ч170
ABI PRISM 310 Genetic Analyzer, setting up run, temperature setting      172
ABI PRISM dRhodamine Terminator Cycle Sequencing Ready Reaction Kit      204
Adenovirus II DNA-Kpnl digest      72
Administrative review, defined      229
Agarose analytical gel, electrophoresis, horizontal gel tank apparatus      48f
Agarose analytical gel, electrophoresis, horizontal submarine gel tank apparatus      62f
Agarose gel      71f
Agarose gel electrophoresis, DNA amplification PCR      107Ч109
Agarose gel electrophoresis, vs. polyacrylamide gel      119Ч120
Agarose template, DNA quantity determination body fluid stains      49Ч50
Alcohol precipitation method      15Ч17
Alcohol precipitation method, body fluid stains      17Ч18
Alcohol precipitation method, liquid blood      15Ч17 16f
Alcohol precipitation method, sperm/vaginal cell      18Ч20
Alkaline phosphatase-labeled probe, slot blot hybridization, DNA quantity determination      53Ч55
Allele designation, STaR CALL      154Ч155
Allele frequency data      245Ч262
Allele frequency datapopulation data examples      247Ч251
Allele-specific oligonucleotide hybridization probe      101
Allelic ladders electropherogram, Profiler Plus      159 159f
Amelogenin, amplicons, silver stained polyacrylamide gel      130f
Amelogenin, fragments      183
Amelogenin, locus, AMP-FLP      122
Amelogenin, locus, DNA amplification      122Ч125
AMP-FLP typing      See Amplified fragment length polymorphism (AMP-FLP) typing
AmpF/STR COfiler      158
AmpF/STR COfiler, allele designation      183
AmpF/STR COfiler, ladder      181f
AmpF/STR Profiler Plus      158
AmpFlSTR Blue Casework      147f
Amplicon, purification, mitochondrial DNA (mtDNA) sequencing      198Ч199
Amplicon, silver-stained polyacrylamide gel, amelogenin      130f
Amplicon, silver-stained polyacrylamide gel, D1S80      130f
Amplification blank control, defined      229
Amplification controls, capillary electrophoresis      160
Amplification reagents, capillary electrophoresis      163
Amplified fragment length, D1S80 locus      122
Amplified fragment length, electrophoresis      128Ч130
Amplified fragment length, polyacrylamide gel, electrophoresis      119Ч121
Amplified fragment length, polymorphism (AMP-FLP), typing      119
Amplified fragment length, polymorphism (AMP-FLP), typing, amelogenin locus      122
Amplified fragment length, polymorphism (AMP-FLP), typing, amplified alleles, electrophoretic separation      126Ч128
Amplified fragment length, silver-stained gels      130Ч131 130f
Amplified fragment length, silver-stained gels, photoduplication      131Ч132
Amplified short tandem repeat loci      138
AmpliTaq Gold DNA Polymerase      96 99f
AmpliTaq Gold DNA Polymerase vs. AmpliTaq      100f
AmpliType PM gel      109f
Analytical gel, RFLP VNTR      72Ч74
Analytical gel, RFLP VNTR, controls      72Ч73
Analytical gel, visual marker      72
Analytical procedures, defined      229
Analytical procedures, quality assurance standards      237Ч239
Anticoagulated blood, dried bloodstains      7Ч8
audits, defined      229
Audits, quality assurance standards      242Ч243
Autoradiography vs. chemiluminescence      66
Autoradiography, RFLP VNTR      65Ч66 67f 81Ч82
Bessman Pulverizer, bone      27Ч28
Bessman Pulverizer, teeth      31Ч32
Biotin      103
Bisacrylamide      120
Blood spot, placement      7f
Blood, anticoagulated, dried bloodstains      7Ч8
Blood, coagulated, reference sample      6
Blood, liquid      See Liquid blood storage
Blood, whole, Chelex method      35Ч36
Blood, whole, salting out method      41
Bloodstains, DNA extraction, Chelex method      35Ч36
Bloodstains, dried, anticoagulated blood      7Ч8
Bloodstains, micron 100 washing, DNA extraction      20Ч22
Blot stripping, chemiluminescent detection RFLPs      90Ч91
Blot stripping, RFLP VNTR      82Ч83 83f
Body fluid stains, DNA extraction, alcohol precipitation method      17Ч18
Body fluid stains, DNA quantity determination ethidium bromide/agarose test gel      47Ч50
Body fluid stains, RFLP      59
Bone, DNA extraction      26Ч29
Bone, DNA extraction, alternate procedure      30Ч31
Bone, DNA extraction, Bessman Pulverizer      27Ч28
Bone, DNA extraction, SPEX 6700 Freezer/Mill      28Ч29
Bone, DNA extraction, while bone marrow accessible      26Ч27
Bovine serum albumin (BSA), PCR      97Ч98
BSA, PCR      97Ч98
Buccal swab, protocol      9Ч11
C.E.P.Swab      10f
Calibration, defined      229
Camera, charge-coupled device      159
Capillary electrophoresis (CE)      157Ч158
Capillary electrophoresis (CE) vs. slab gel      157
Capillary electrophoresis (CE), ABI Prism 310 Genetic Analyzer      162 167Ч176
Capillary electrophoresis (CE), ABI Prism 310 Genetic Analyzer, setting up      167Ч168
Capillary electrophoresis (CE), allele designation      183Ч187
Capillary electrophoresis (CE), amplification, controls      160
Capillary electrophoresis (CE), amplification, reagents      163
Capillary electrophoresis (CE), apparatus      158
Capillary electrophoresis (CE), data examination      179Ч183
Capillary electrophoresis (CE), DNA quantification, procedure      164Ч166
Capillary electrophoresis (CE), DNA sample injection      158
Capillary electrophoresis (CE), equipment      161
Capillary electrophoresis (CE), extraction controls      160
Capillary electrophoresis (CE), GeneAmp PCR System 9700 Thermal Cycler      161
Capillary electrophoresis (CE), GeneScan analysis      176
Capillary electrophoresis (CE), Genotyper analysis      177Ч178
Capillary electrophoresis (CE), GS500[ROX]      163Ч164
Capillary electrophoresis (CE), mitochondrial DNA (mtDNA) sequencing      193
Capillary electrophoresis (CE), pipets      162
Capillary electrophoresis (CE), power Macintosh computers      162
Capillary electrophoresis (CE), quality control      160 163
Capillary electrophoresis (CE), reagents      163
Capillary electrophoresis (CE), sieving medium      158
Capillary electrophoresis (CE), STR typing      166Ч167
Capillary electrophoresis (CE), using template file      179
Capillary preparation, mitochondrial DNA (mtDNA) sequencing      199Ч200
CCD camera      159
CE      See Capillary electrophoresis
Centrifuge column preparation, mitochondrial DNA (mtDNA), sequencing      204
Charge-coupled device (CCD), camera      159
Chelex method, DNA extraction      14
Chelex method, DNA extraction, cigarette butts      38
Chelex method, DNA extraction, envelope flaps      38
Chelex method, DNA extraction, saliva      37
Chelex method, DNA extraction, semen-containing stains      36Ч37
Chelex method, DNA extraction, stamps      38
Chelex method, DNA extraction, whole blood or bloodstains      35Ч36
Chemiluminescence vs. autoradiography      66
Chemiluminescence, RFLP VNTR      66Ч68 67f
Chemiluminescence, slot blot hybridization, DNA quantity determination      53Ч55 55f
Chemiluminescent detection, restriction fragment-length, polymorphism      84Ч91
Chemiluminescent detection, restriction fragment-length, polymorphism, blot stripping      90Ч91
Chemiluminescent detection, restriction fragment-length, polymorphism, electrophoresis      84
Chemiluminescent detection, restriction fragment-length, polymorphism, hybridization      86Ч88
Chemiluminescent detection, restriction fragment-length, polymorphism, hybridization, volumes      87f 89f
Chemiluminescent detection, restriction fragment-length, polymorphism, lumography      90 90f
Chemiluminescent detection, restriction fragment-length, polymorphism, post-hybridization washes      88Ч90
Chemiluminescent detection, restriction fragment-length, polymorphism, southern blotting      85Ч86
Cigarette butts, DNA extraction      24
Cigarette butts, DNA extraction, Chelex method      38
Coagulated blood, reference sample      6
CODIS, STR core loci      136
CODIS, STR core loci, characteristics      136f
Color development, PCR      113Ч114
Combined DNA Index System (CODIS), STR core loci      136
Combined DNA Index System (CODIS), STR core loci, characteristics      136f
Commercial test kit, defined      229
Continuing education personnel, quality assurance standards      232
Convertible Filtration Manifold System      51f
Corrective action, quality assurance standards      242
Critical reagents, defined      229
CSF1PO, locus      259
Cycle sequencing reaction, mitochondrial DNA (mtDNA), sequencing      2Q4Ч205 206f
Cycle test, GeneAmp PCR System 9700 Thermal Cycler      161
D10S28 locus, allele frequency data      250
D13S317, locus      258Ч259
D16S539, locus      260
D17S79 locus, allele frequency data      251
D18S51, locus      257Ч258
D1S7 locus, allele frequency data      247
D1S80 amplicons, silver stained polyacrylamide gel      130f
D1S80 locus, AMP-FLP      122
D1S80, allele frequencies      254
D21S11, locus      257
D2S44 locus, allele frequency data      248
D3S13581, locus      255
D4S139 locus, allele frequency data      249
D5S818, locus      258
D7S829, locus      259
D8S1179, locus      256
Decree, examiner/analyst      234
Decree, laboratory leader      233
Discontinuous buffer system      121
DNA 9947A      160
DNA amplification, amelogenin locus      122Ч125
DNA amplification, PCR      101Ч105
DNA amplification, PCR, agarose gel electrophoresis, evaluation      107Ч109
DNA amplification, PCR, agarose gel electrophoresis, HLA-DQA1 typing      101Ч107 102f
DNA amplification, PCR, agarose gel electrophoresis, PM typing      101Ч107 102f
DNA analysis, sexual assault      61f
DNA extraction      13Ч42
DNA extraction, alcohol precipitation method, body fluid stains      17Ч18
DNA extraction, alcohol precipitation method, liquid blood      15Ч17
DNA extraction, alcohol precipitation method, sperm/vaginal cell      18Ч20
DNA extraction, bone      26Ч29
DNA extraction, bone, alternate procedure      30Ч31
DNA extraction, bone, Bessman Pulverizer      27Ч28
DNA extraction, bone, SPEX 6700 Freezer/Mill      28Ч29
DNA extraction, bone, while bone marrow accessible      26Ч27
DNA extraction, Chelex method      14
DNA extraction, Chelex method, cigarette butts      24Ч25 38
DNA extraction, Chelex method, envelope flaps      24 38
DNA extraction, Chelex method, gemen-containing stains      36Ч37
DNA extraction, Chelex method, saliva      37
DNA extraction, FTA paper      41Ч42
DNA extraction, hair      25 38Ч40
DNA extraction, hair, DNA extraction procedure      39Ч40
DNA extraction, hair, grinding hair      39
DNA extraction, micron 100 washing, bloodstains and saliva stains      20Ч22
DNA extraction, micron 100 washing, vaginal swabs or semen stains      22Ч23
DNA extraction, nonorganic procedure      14 41
DNA extraction, organic      13Ч24
DNA extraction, purity      13
DNA extraction, rape      14Ч15
DNA extraction, salting out method, whole blood      41
DNA extraction, soft tissues      24Ч25
DNA extraction, stamps      24 38
DNA extraction, teeth      31Ч35
DNA extraction, teeth, Bessman Pulverizer      32Ч33
DNA extraction, teeth, SPEX 6700 Freezer/Mill      33Ч35
DNA extraction, teeth, with soft tissue present      31Ч32
DNA extraction, whole blood pr bloodstains      35Ч36
DNA hybridization, PCR      109Ч113
DNA Identification Act of 1994      227
DNA probe, high-stringency washes      82f
DNA probe, RFLP VNTR, commercially available      78
DNA probe, RFLP VNTR, radiolabeling      76Ч78 77f
DNA probe, volume calculations      79f
DNA quantification, capillary electrophoresis      164Ч166
DNA quantity determination      45Ч55
DNA quantity determination, ethidium bromide/agarose test gel body fluid stains      47Ч50
DNA quantity determination, slot blot hybridization      46
DNA quantity determination, slot blot hybridization, $^{32}P$-labeled alphoid probe      50Ч53
DNA quantity determination, slot blot hybridization, alkaline phosphatase (AP)-labeled probe      53Ч55
DNA quantity determination, slot blot hybridization, chemiluminescence      53Ч55 55f
DNA quantity determination, spectrophotometry      45
DNA quantity determination, spectrophotometry, liquid blood      46Ч47
dRhodamine chemistry      211
Dried bloodstains, anticoagulated blood      7Ч8
Dried bloodstains, reference sample      6
Duties, laboratory leader      233Ч234
Dye Terminator chemistry      211
EcoRI, restriction digestion      58f
Education, personnel, quality assurance standards      232
EEO      157
Electroendosmosis (EEO)      157
Electrokinetic injection      158
Electropherogram, allelic ladders, Profiler Plus      159 159f 183
Electrophoresis, AMP-FLP      128Ч130
Electrophoresis, amplified alleles      126Ч128
Electrophoresis, amplified alleles, polyacrylamide gel      126Ч128 127f
Electrophoresis, capillary      See Capillary electrophoresis
Electrophoresis, chemiluminescent detection RFLPs      84
Electrophoresis, gel      See Gel electrophoresis mitochondrial DNA (mtDNA) sequencing
Electrophoresis, gel, sample preparation      206
Electrophoresis, STR      143Ч144
Electrophoresis, variable number tandem repeat loci, RFLP      60
Envelope flaps, DNA extraction      24
Envelope flaps, DNA extraction, Chelex method      38
Ethidium bromide vs. silver stains      121Ч122
Ethidium bromide/agarose test gel, DNA quantity determination, body fluid stains      47Ч50
Evidence control, quality assurance standards      235
Examiner/analyst, defined      229
Examiner/analyst, requirements, quality assurance standards      234
Experience, examiner/analyst      234
Experience, laboratory leader      233
Extraction controls, capillary electrophoresis      160
Facilities, quality assurance standards      235
FGA, locus      255Ч256
Fluorescent detection-based, automated sequencer      193
FMBIO ANALYSIS 8.0, multicolor gel analysis      148Ч154
FMBIO II fluorescent scanner, polyacrylamide gel analysis      144Ч148 147f
Foam applicator swab      11f
Forensic DNA testing, defined      230
Forensic DNA testing, laboratories quality assurance standards      See Quality assurance standards forensic
Formamide      163
FTA paper      8f
FTA paper, DNA extraction      41Ч42
FTA paper, DNA extraction, protocol      8Ч9
Gel electrophoresis, agarose analytical, horizontal gel tank apparatus      48f
Gel electrophoresis, agarose analytical, horizontal submarine gel tank apparatus      62f
Gel electrophoresis, agarose, DNA amplification PCR      107Ч109
Gel electrophoresis, agarose, vs. polyacrylamide gel      119Ч120
Gel preparation, mitochondrial DNA (mtDNA) sequencing      207Ч210
Gel tank apparatus, horizontal submarine agarose analytical gel electrophoresis      62f
Gel tank apparatus, horizontal, agarose analytical gel electrophoresis      48f
Gene Guard swab      10f
GenePrint PowerPlex      145fЧ146f
GenePrint PowerPlex, autoband settings      152t
GenePrint PowerPlex, multi-band separation parameter settings      149t
GeneScan Analysis      176 176fЧ177f 183
GeneScan Sample Sheet      173 174fЧ175f
GenoTyper analysis      177Ч178
GenoTyper Application      183
GenoTyper Application, main window      182f
GenoTyper Plot window      182f
GS500[ROX]      163Ч164 183
1 2 3
blank
–еклама
blank
blank
HR
@Mail.ru
       © Ёлектронна€ библиотека попечительского совета мехмата ћ√”, 2004-2020
Ёлектронна€ библиотека мехмата ћ√” | Valid HTML 4.01! | Valid CSS! ќ проекте