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| Ïîèñê ïî óêàçàòåëÿì |
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| Budowle B. — DNA Typing Protocols |
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| Ïðåäìåòíûé óêàçàòåëü |
 -labeled alphoid probe, slot blot hybridization, DNA quantity determination 50—53
ABI PRISM 310 Genetic Analyzer 159 167f 184f
ABI PRISM 310 Genetic Analyzer, buffer and waste vials 162
ABI PRISM 310 Genetic Analyzer, capillary 162
ABI PRISM 310 Genetic Analyzer, electrode installation 168 168f
ABI PRISM 310 Genetic Analyzer, honing 167—168
ABI PRISM 310 Genetic Analyzer, matrix 162
ABI PRISM 310 Genetic Analyzer, response evaluation 162
ABI PRISM 310 Genetic Analyzer, setting up run 169—176
ABI PRISM 310 Genetic Analyzer, setting up run, autosampler calibration 171
ABI PRISM 310 Genetic Analyzer, setting up run, buffer reservoir filling 171—172
ABI PRISM 310 Genetic Analyzer, setting up run, capillary installation 170—171
ABI PRISM 310 Genetic Analyzer, setting up run, priming 172
ABI PRISM 310 Genetic Analyzer, setting up run, pump block 170
ABI PRISM 310 Genetic Analyzer, setting up run, sample preparation 172
ABI PRISM 310 Genetic Analyzer, setting up run, syringe 169—170
ABI PRISM 310 Genetic Analyzer, setting up run, temperature setting 172
ABI PRISM dRhodamine Terminator Cycle Sequencing Ready Reaction Kit 204
Adenovirus II DNA-Kpnl digest 72
Administrative review, defined 229
Agarose analytical gel, electrophoresis, horizontal gel tank apparatus 48f
Agarose analytical gel, electrophoresis, horizontal submarine gel tank apparatus 62f
Agarose gel 71f
Agarose gel electrophoresis, DNA amplification PCR 107—109
Agarose gel electrophoresis, vs. polyacrylamide gel 119—120
Agarose template, DNA quantity determination body fluid stains 49—50
Alcohol precipitation method 15—17
Alcohol precipitation method, body fluid stains 17—18
Alcohol precipitation method, liquid blood 15—17 16f
Alcohol precipitation method, sperm/vaginal cell 18—20
Alkaline phosphatase-labeled probe, slot blot hybridization, DNA quantity determination 53—55
Allele designation, STaR CALL 154—155
Allele frequency data 245—262
Allele frequency datapopulation data examples 247—251
Allele-specific oligonucleotide hybridization probe 101
Allelic ladders electropherogram, Profiler Plus 159 159f
Amelogenin, amplicons, silver stained polyacrylamide gel 130f
Amelogenin, fragments 183
Amelogenin, locus, AMP-FLP 122
Amelogenin, locus, DNA amplification 122—125
AMP-FLP typing See Amplified fragment length polymorphism (AMP-FLP) typing
AmpF/STR COfiler 158
AmpF/STR COfiler, allele designation 183
AmpF/STR COfiler, ladder 181f
AmpF/STR Profiler Plus 158
AmpFlSTR Blue Casework 147f
Amplicon, purification, mitochondrial DNA (mtDNA) sequencing 198—199
Amplicon, silver-stained polyacrylamide gel, amelogenin 130f
Amplicon, silver-stained polyacrylamide gel, D1S80 130f
Amplification blank control, defined 229
Amplification controls, capillary electrophoresis 160
Amplification reagents, capillary electrophoresis 163
Amplified fragment length, D1S80 locus 122
Amplified fragment length, electrophoresis 128—130
Amplified fragment length, polyacrylamide gel, electrophoresis 119—121
Amplified fragment length, polymorphism (AMP-FLP), typing 119
Amplified fragment length, polymorphism (AMP-FLP), typing, amelogenin locus 122
Amplified fragment length, polymorphism (AMP-FLP), typing, amplified alleles, electrophoretic separation 126—128
Amplified fragment length, silver-stained gels 130—131 130f
Amplified fragment length, silver-stained gels, photoduplication 131—132
Amplified short tandem repeat loci 138
AmpliTaq Gold DNA Polymerase 96 99f
AmpliTaq Gold DNA Polymerase vs. AmpliTaq 100f
AmpliType PM gel 109f
Analytical gel, RFLP VNTR 72—74
Analytical gel, RFLP VNTR, controls 72—73
Analytical gel, visual marker 72
Analytical procedures, defined 229
Analytical procedures, quality assurance standards 237—239
Anticoagulated blood, dried bloodstains 7—8
audits, defined 229
Audits, quality assurance standards 242—243
Autoradiography vs. chemiluminescence 66
Autoradiography, RFLP VNTR 65—66 67f 81—82
Bessman Pulverizer, bone 27—28
Bessman Pulverizer, teeth 31—32
Biotin 103
Bisacrylamide 120
Blood spot, placement 7f
Blood, anticoagulated, dried bloodstains 7—8
Blood, coagulated, reference sample 6
Blood, liquid See Liquid blood storage
Blood, whole, Chelex method 35—36
Blood, whole, salting out method 41
Bloodstains, DNA extraction, Chelex method 35—36
Bloodstains, dried, anticoagulated blood 7—8
Bloodstains, micron 100 washing, DNA extraction 20—22
Blot stripping, chemiluminescent detection RFLPs 90—91
Blot stripping, RFLP VNTR 82—83 83f
Body fluid stains, DNA extraction, alcohol precipitation method 17—18
Body fluid stains, DNA quantity determination ethidium bromide/agarose test gel 47—50
Body fluid stains, RFLP 59
Bone, DNA extraction 26—29
Bone, DNA extraction, alternate procedure 30—31
Bone, DNA extraction, Bessman Pulverizer 27—28
Bone, DNA extraction, SPEX 6700 Freezer/Mill 28—29
Bone, DNA extraction, while bone marrow accessible 26—27
Bovine serum albumin (BSA), PCR 97—98
BSA, PCR 97—98
Buccal swab, protocol 9—11
C.E.P.Swab 10f
Calibration, defined 229
Camera, charge-coupled device 159
Capillary electrophoresis (CE) 157—158
Capillary electrophoresis (CE) vs. slab gel 157
Capillary electrophoresis (CE), ABI Prism 310 Genetic Analyzer 162 167—176
Capillary electrophoresis (CE), ABI Prism 310 Genetic Analyzer, setting up 167—168
Capillary electrophoresis (CE), allele designation 183—187
Capillary electrophoresis (CE), amplification, controls 160
Capillary electrophoresis (CE), amplification, reagents 163
Capillary electrophoresis (CE), apparatus 158
Capillary electrophoresis (CE), data examination 179—183
Capillary electrophoresis (CE), DNA quantification, procedure 164—166
Capillary electrophoresis (CE), DNA sample injection 158
Capillary electrophoresis (CE), equipment 161
Capillary electrophoresis (CE), extraction controls 160
Capillary electrophoresis (CE), GeneAmp PCR System 9700 Thermal Cycler 161
Capillary electrophoresis (CE), GeneScan analysis 176
Capillary electrophoresis (CE), Genotyper analysis 177—178
Capillary electrophoresis (CE), GS500[ROX] 163—164
Capillary electrophoresis (CE), mitochondrial DNA (mtDNA) sequencing 193
Capillary electrophoresis (CE), pipets 162
Capillary electrophoresis (CE), power Macintosh computers 162
Capillary electrophoresis (CE), quality control 160 163
Capillary electrophoresis (CE), reagents 163
Capillary electrophoresis (CE), sieving medium 158
Capillary electrophoresis (CE), STR typing 166—167
Capillary electrophoresis (CE), using template file 179
Capillary preparation, mitochondrial DNA (mtDNA) sequencing 199—200
CCD camera 159
CE See Capillary electrophoresis
Centrifuge column preparation, mitochondrial DNA (mtDNA), sequencing 204
Charge-coupled device (CCD), camera 159
Chelex method, DNA extraction 14
Chelex method, DNA extraction, cigarette butts 38
Chelex method, DNA extraction, envelope flaps 38
Chelex method, DNA extraction, saliva 37
Chelex method, DNA extraction, semen-containing stains 36—37
Chelex method, DNA extraction, stamps 38
Chelex method, DNA extraction, whole blood or bloodstains 35—36
Chemiluminescence vs. autoradiography 66
Chemiluminescence, RFLP VNTR 66—68 67f
Chemiluminescence, slot blot hybridization, DNA quantity determination 53—55 55f
Chemiluminescent detection, restriction fragment-length, polymorphism 84—91
Chemiluminescent detection, restriction fragment-length, polymorphism, blot stripping 90—91
Chemiluminescent detection, restriction fragment-length, polymorphism, electrophoresis 84
Chemiluminescent detection, restriction fragment-length, polymorphism, hybridization 86—88
Chemiluminescent detection, restriction fragment-length, polymorphism, hybridization, volumes 87f 89f
Chemiluminescent detection, restriction fragment-length, polymorphism, lumography 90 90f
Chemiluminescent detection, restriction fragment-length, polymorphism, post-hybridization washes 88—90
| Chemiluminescent detection, restriction fragment-length, polymorphism, southern blotting 85—86
Cigarette butts, DNA extraction 24
Cigarette butts, DNA extraction, Chelex method 38
Coagulated blood, reference sample 6
CODIS, STR core loci 136
CODIS, STR core loci, characteristics 136f
Color development, PCR 113—114
Combined DNA Index System (CODIS), STR core loci 136
Combined DNA Index System (CODIS), STR core loci, characteristics 136f
Commercial test kit, defined 229
Continuing education personnel, quality assurance standards 232
Convertible Filtration Manifold System 51f
Corrective action, quality assurance standards 242
Critical reagents, defined 229
CSF1PO, locus 259
Cycle sequencing reaction, mitochondrial DNA (mtDNA), sequencing 2Q4—205 206f
Cycle test, GeneAmp PCR System 9700 Thermal Cycler 161
D10S28 locus, allele frequency data 250
D13S317, locus 258—259
D16S539, locus 260
D17S79 locus, allele frequency data 251
D18S51, locus 257—258
D1S7 locus, allele frequency data 247
D1S80 amplicons, silver stained polyacrylamide gel 130f
D1S80 locus, AMP-FLP 122
D1S80, allele frequencies 254
D21S11, locus 257
D2S44 locus, allele frequency data 248
D3S13581, locus 255
D4S139 locus, allele frequency data 249
D5S818, locus 258
D7S829, locus 259
D8S1179, locus 256
Decree, examiner/analyst 234
Decree, laboratory leader 233
Discontinuous buffer system 121
DNA 9947A 160
DNA amplification, amelogenin locus 122—125
DNA amplification, PCR 101—105
DNA amplification, PCR, agarose gel electrophoresis, evaluation 107—109
DNA amplification, PCR, agarose gel electrophoresis, HLA-DQA1 typing 101—107 102f
DNA amplification, PCR, agarose gel electrophoresis, PM typing 101—107 102f
DNA analysis, sexual assault 61f
DNA extraction 13—42
DNA extraction, alcohol precipitation method, body fluid stains 17—18
DNA extraction, alcohol precipitation method, liquid blood 15—17
DNA extraction, alcohol precipitation method, sperm/vaginal cell 18—20
DNA extraction, bone 26—29
DNA extraction, bone, alternate procedure 30—31
DNA extraction, bone, Bessman Pulverizer 27—28
DNA extraction, bone, SPEX 6700 Freezer/Mill 28—29
DNA extraction, bone, while bone marrow accessible 26—27
DNA extraction, Chelex method 14
DNA extraction, Chelex method, cigarette butts 24—25 38
DNA extraction, Chelex method, envelope flaps 24 38
DNA extraction, Chelex method, gemen-containing stains 36—37
DNA extraction, Chelex method, saliva 37
DNA extraction, FTA paper 41—42
DNA extraction, hair 25 38—40
DNA extraction, hair, DNA extraction procedure 39—40
DNA extraction, hair, grinding hair 39
DNA extraction, micron 100 washing, bloodstains and saliva stains 20—22
DNA extraction, micron 100 washing, vaginal swabs or semen stains 22—23
DNA extraction, nonorganic procedure 14 41
DNA extraction, organic 13—24
DNA extraction, purity 13
DNA extraction, rape 14—15
DNA extraction, salting out method, whole blood 41
DNA extraction, soft tissues 24—25
DNA extraction, stamps 24 38
DNA extraction, teeth 31—35
DNA extraction, teeth, Bessman Pulverizer 32—33
DNA extraction, teeth, SPEX 6700 Freezer/Mill 33—35
DNA extraction, teeth, with soft tissue present 31—32
DNA extraction, whole blood pr bloodstains 35—36
DNA hybridization, PCR 109—113
DNA Identification Act of 1994 227
DNA probe, high-stringency washes 82f
DNA probe, RFLP VNTR, commercially available 78
DNA probe, RFLP VNTR, radiolabeling 76—78 77f
DNA probe, volume calculations 79f
DNA quantification, capillary electrophoresis 164—166
DNA quantity determination 45—55
DNA quantity determination, ethidium bromide/agarose test gel body fluid stains 47—50
DNA quantity determination, slot blot hybridization 46
DNA quantity determination, slot blot hybridization, -labeled alphoid probe 50—53
DNA quantity determination, slot blot hybridization, alkaline phosphatase (AP)-labeled probe 53—55
DNA quantity determination, slot blot hybridization, chemiluminescence 53—55 55f
DNA quantity determination, spectrophotometry 45
DNA quantity determination, spectrophotometry, liquid blood 46—47
dRhodamine chemistry 211
Dried bloodstains, anticoagulated blood 7—8
Dried bloodstains, reference sample 6
Duties, laboratory leader 233—234
Dye Terminator chemistry 211
EcoRI, restriction digestion 58f
Education, personnel, quality assurance standards 232
EEO 157
Electroendosmosis (EEO) 157
Electrokinetic injection 158
Electropherogram, allelic ladders, Profiler Plus 159 159f 183
Electrophoresis, AMP-FLP 128—130
Electrophoresis, amplified alleles 126—128
Electrophoresis, amplified alleles, polyacrylamide gel 126—128 127f
Electrophoresis, capillary See Capillary electrophoresis
Electrophoresis, chemiluminescent detection RFLPs 84
Electrophoresis, gel See Gel electrophoresis mitochondrial DNA (mtDNA) sequencing
Electrophoresis, gel, sample preparation 206
Electrophoresis, STR 143—144
Electrophoresis, variable number tandem repeat loci, RFLP 60
Envelope flaps, DNA extraction 24
Envelope flaps, DNA extraction, Chelex method 38
Ethidium bromide vs. silver stains 121—122
Ethidium bromide/agarose test gel, DNA quantity determination, body fluid stains 47—50
Evidence control, quality assurance standards 235
Examiner/analyst, defined 229
Examiner/analyst, requirements, quality assurance standards 234
Experience, examiner/analyst 234
Experience, laboratory leader 233
Extraction controls, capillary electrophoresis 160
Facilities, quality assurance standards 235
FGA, locus 255—256
Fluorescent detection-based, automated sequencer 193
FMBIO ANALYSIS 8.0, multicolor gel analysis 148—154
FMBIO II fluorescent scanner, polyacrylamide gel analysis 144—148 147f
Foam applicator swab 11f
Forensic DNA testing, defined 230
Forensic DNA testing, laboratories quality assurance standards See Quality assurance standards forensic
Formamide 163
FTA paper 8f
FTA paper, DNA extraction 41—42
FTA paper, DNA extraction, protocol 8—9
Gel electrophoresis, agarose analytical, horizontal gel tank apparatus 48f
Gel electrophoresis, agarose analytical, horizontal submarine gel tank apparatus 62f
Gel electrophoresis, agarose, DNA amplification PCR 107—109
Gel electrophoresis, agarose, vs. polyacrylamide gel 119—120
Gel preparation, mitochondrial DNA (mtDNA) sequencing 207—210
Gel tank apparatus, horizontal submarine agarose analytical gel electrophoresis 62f
Gel tank apparatus, horizontal, agarose analytical gel electrophoresis 48f
Gene Guard swab 10f
GenePrint PowerPlex 145f—146f
GenePrint PowerPlex, autoband settings 152t
GenePrint PowerPlex, multi-band separation parameter settings 149t
GeneScan Analysis 176 176f—177f 183
GeneScan Sample Sheet 173 174f—175f
GenoTyper analysis 177—178
GenoTyper Application 183
GenoTyper Application, main window 182f
GenoTyper Plot window 182f
GS500[ROX] 163—164 183
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