Budowle B. — DNA Typing Protocols :: Электронная библиотека попечительского совета мехмата МГУ

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Budowle B. — DNA Typing Protocols

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Название: DNA Typing Protocols

Автор: Budowle B.

Аннотация:

(BioTechniques Books) FBI, Washington, DC. Laboratory manual provides a comprehensive compendium of DNA typing techniques. Compiled by a team of prominent biomolecular scientists from the FBI Forensic Sciences Research and Training Laboratory. Topics include DNA extraction techniques. Outline format. Plastic-comb binding.

Язык:

Рубрика: Технология/

Статус предметного указателя: Готов указатель с номерами страниц

ed2k: ed2k stats

Год издания: 2000

Количество страниц: 304

Добавлена в каталог: 21.11.2006

Операции: Положить на полку | Скопировать ссылку для форума | Скопировать ID

Предметный указатель

HaeIII, liquid blood, RFLP VNTR      69—70
HaeIII, restriction digestion      58f
HaeIII, VNTR      59t
HaeIII-digested DNA      68f
HaeIII-digested K562 DNA      73
Hair specimens, slide-mounted, DNA extraction      25
Hair, DNA extraction      25 38—40
Hair, DNA extraction, grinding hair      39
Hair, DNA extraction, procedure      39—40
Hair, mtDNA      191
Heteroplasmy      191
High-stringency washes, DNA probes      82f
HLA-DQA1 loci, amplified products      109f
HLA-DQA1 loci, characteristics      104f
HLA-DQA1 loci, PCR      101—102 102f
HLA-DQA1, allele frequencies      252
Horizontal gel tank apparatus agarose analytical gel electrophoresis      48f
Horizontal submarine gel tank, apparatus, agarose analytical gel electrophoresis      62f
Human DNA control      73
Hybridization, chemiluminescent detection RFLPs      86—88
Hybridization, PCR      109—113
Hybridization, RFLP VNTR      79
Hybridization, RFLP VNTR, membrane      64—65
Hybridization, slot blot, DNA quantity determination      46 50—55 55f
Hybridization, solution, preparation      80f
Hybridization, solution, radioactive viral DNA concentrations      80f
Hydrodynamic injection      158
Immigration, RFLP      59
Job description, personnel, quality assurance standards      232
Known samples, defined      230
Laboratory support, defined      230
Laboratory, defined      230
Liquid blood, collection      5
Liquid blood, DNA extraction, alcohol precipitation method      15—17
Liquid blood, HaeIII, RFLP VNTR      69—70
Liquid blood, spectrophotometry, DNA quantity determination      46—47
Local Southern analysis      183
Lumi-Phos Plus      66
Lumography, chemiluminescent detection RFLPs      90 90f
Manager, requirements, quality assurance standards      232—233
Membranes, hybridization, RFLP variable number, tandem repeat loci      64—65
Membranes, hybridization, RFLP VNTR      64—65
Membranes, variable number tandem repeat, loci, RFLP      62
Micro Tissue Grinders      39
Micron 100 washing, DNA extraction, bloodstains and saliva stains      20—22
Micron 100 washing, DNA extraction, vaginal swabs or semen, stains      22—23
Micron device      21f
Minigel reagents      108f
Minisatellites      57
Mitochondrial DNA (mtDNA)      190f
Mitochondrial DNA (mtDNA) vs. nuclear DNA      189 191
Mitochondrial DNA (mtDNA), hair      38—10
Mitochondrial DNA (mtDNA), maternal inheritance      190f
Mitochondrial DNA (mtDNA), primers, naming      194—195
Mitochondrial DNA (mtDNA), primers, routine reference samples      195 195f
Mitochondrial DNA (mtDNA), region HV1, reference sequence      261—262
Mitochondrial DNA (mtDNA), region HV2, reference sequence      262
Mitochondrial DNA (mtDNA), sequencing      189—224 192f
Mitochondrial DNA (mtDNA), sequencing, amplicon purification      198—199
Mitochondrial DNA (mtDNA), sequencing, amplification      194—196
Mitochondrial DNA (mtDNA), sequencing, amplification, procedure      196—197 197f 198t
Mitochondrial DNA (mtDNA), sequencing, chromatogram      216f
Mitochondrial DNA (mtDNA), sequencing, cleaning plates, comb, spacers      216—217
Mitochondrial DNA (mtDNA), sequencing, gel loading      216
Mitochondrial DNA (mtDNA), sequencing, instrument setup      212—215 213f 215f
Mitochondrial DNA (mtDNA), sequencing, Model 377 DNA Sequencer      206—207
Mitochondrial DNA (mtDNA), sequencing, post-amplification, quantification      199—203
Mitochondrial DNA (mtDNA), sequencing, post-amplification, quantitation      193
Mitochondrial DNA (mtDNA), sequencing, post-electrophoresis analysis      217
Mitochondrial DNA (mtDNA), sequencing, quality assurance      194
Mitochondrial DNA (mtDNA), sequencing, quantity determination      203
Mitochondrial DNA (mtDNA), sequencing, sample preparation      203—206 215—216
Mitochondrial DNA (mtDNA), sequencing, sample preparation, electrophoresis      206
Mitochondrial DNA (mtDNA), sequencing, sample sheet and run file      210—212 210f—212f
Mitochondrial DNA (mtDNA), sequencing, Sequence Navigator Software      217—224 217f—223f
Mitochondrial DNA (mtDNA), sequencing, sequencing gel preparation      207—210
Model 377 DNA Sequencer      206—207 208f
Molecular weight markers      72
mtDNA      See Mitochondrial DNA
Multicolor gel analysis, FMBIO 8.0      148—154
Multicolor gel analysis, FMBIO ANALYSIS 8.0      148—154
National Institute of Standards and Technology (NIST)      230
Nitrocellulose      62
Nonorganic procedure, DNA extraction      14
Nylon      62 72—74 85—86
Omnibus Crime Control Act      227
Organic procedure, DNA extraction      13—14
Organic procedure, DNA extraction, alcohol precipitation method      15—17
P/ACE Detector Time Program      201f
Paternity, RFLP      59 60f
PCR      See Polymerase chain reaction
PDA      120
Performance Optimized Polymer 4 (POP-4), Genetic Analyzer Buffer with EDTA      164
Personnel, quality assurance standards      232—233
Photography, PCR      114—115
Piperazine diacrylamide (PDA)      120
PM      See Polymarker
Polyacrylamide gels vs. agarose gel electrophoresis      119—120
Polyacrylamide gels, AMP-FLP, silver staining      130—132 130f
Polyacrylamide gels, analysis, FMBIO II      144—148
Polyacrylamide gels, casting      127f
Polyacrylamide gels, electrophoresis, AMP-FLP      119—121
Polyacrylamide gels, electrophoresis, AMP-FLP, advantages      120
Polyacrylamide gels, electrophoresis, amplified alleles      126—128 127f
Polyacrylamide gels, STR      142—143
Polyacrylamide gels, wells      129f
Polymarker (PM), allele frequencies      252—253
Polymarker (PM), loci, amplified products      109f
Polymarker (PM), loci, characteristics      104f
Polymarker (PM), loci, PCR      101—107 102f
PolyMarker typing strips, typing tray      112f
Polymerase chain reaction (PCR)      95—101 97f
Polymerase chain reaction (PCR) vs. RFLP      95
Polymerase chain reaction (PCR), advantages      95
Polymerase chain reaction (PCR), annealing temperature      96
Polymerase chain reaction (PCR), bovine serum albumin      97—98
Polymerase chain reaction (PCR), color development      113—114
Polymerase chain reaction (PCR), contaminants      97—98
Polymerase chain reaction (PCR), contamination, prevention      98
Polymerase chain reaction (PCR), defined      230
Polymerase chain reaction (PCR), diagram      97f
Polymerase chain reaction (PCR), DNA amplification      101—107
Polymerase chain reaction (PCR), DNA amplification, agarose gel electrophoresis evaluation      107—109
Polymerase chain reaction (PCR), DNA amplification, HLA-DQA1 typing      101—107 102f
Polymerase chain reaction (PCR), DNA amplification, PM typing      101—107 102f
Polymerase chain reaction (PCR), DNA hybridization      109—113
Polymerase chain reaction (PCR), DNA quantity determination      45
Polymerase chain reaction (PCR), materials analyzed      95
Polymerase chain reaction (PCR), photography      114—115
Polymerase chain reaction (PCR), primers      96
Polymerase chain reaction (PCR), reagents      96
Polymerase chain reaction (PCR), strips storage      114—115
Polymerase chain reaction (PCR), three-temperature      98f
POP-4, Genetic Analyzer Buffer with EDTA      164
Post-hybridization washes, chemiluminescent detection RFLPs      88—90
Post-hybridization washes, RFLP VNTR      65 81
Post-restriction test gel      71—72
Power Macintosh computers      162
Probe labeling, VNTR loci RFLP      64
Probe, alkaline phosphatase (AP)-labeled, slot blot hybridization      53—55
Probe, allele-specific oligonucleotide (ASO) hybridization      101
Probe, DNA, $^{32}P$ -labeled alphoid slot blot hybridization      50—53
Probe, DNA, $^{32}P$ -labeled alphoid slot blot hybridization, variable number tandem, repeat loci, RFLP      63—64 64f
Probe, DNA, commercially available      78
Probe, DNA, high-stringency washes      82f
Probe, DNA, radiolabeling      76—78 77f
Proficiency test sample, defined      230
Proficiency testing, defined      230
Proficiency testing, quality assurance standards      241—242
Profiler Plus, CE, allelic designation      183

Profiler Plus, CE, allelic ladders      180f
Profiler Plus, electropherogram, allelic ladders      159 159f
Qualifying test, defined      230
Quality assurance program quality assurance standards      231—232
Quality assurance standards, forensic DNA testing, laboratories      227—243
Quality assurance standards, forensic DNA testing, laboratories, analytical procedures      237—239
Quality assurance standards, forensic DNA testing, laboratories, audits      242—243
Quality assurance standards, forensic DNA testing, laboratories, change mechanism      228
Quality assurance standards, forensic DNA testing, laboratories, corrective action      242
Quality assurance standards, forensic DNA testing, laboratories, definitions      229—231
Quality assurance standards, forensic DNA testing, laboratories, equipment      239
Quality assurance standards, forensic DNA testing, laboratories, evidence control      235
Quality assurance standards, forensic DNA testing, laboratories, facilities      235
Quality assurance standards, forensic DNA testing, laboratories, organization and management      232
Quality assurance standards, forensic DNA testing, laboratories, personnel      232—233
Quality assurance standards, forensic DNA testing, laboratories, proficiency testing      241—242
Quality assurance standards, forensic DNA testing, laboratories, quality assurance program      231—232
Quality assurance standards, forensic DNA testing, laboratories, references      228—229
Quality assurance standards, forensic DNA testing, laboratories, reports      240
Quality assurance standards, forensic DNA testing, laboratories, review      240
Quality assurance standards, forensic DNA testing, laboratories, scope      229
Quality assurance standards, forensic DNA testing, laboratories, validation      235—237
Quality assurance, defined      230
Quality assurance, mitochondrial DNA (mtDNA), sequencing      194
Quality control, capillary electrophoresis      160 163
Quality control, mitochondrial DNA (mtDNA) sequencing      194
Quality manual, defined      230
Quality system, defined      230
Radioactive viral DNA, concentrations, hybridization solution      80f
Rape, DNA extraction      14—15
Rate test, GeneAmp PCR System 9700 Thermal Cycler      161
Reagent blank control, defined      230
Reagents      269—292
Reagents, capillary electrophoresis      163
Reference material, defined      230
Reference samples      5
Reference samples, collection and storage      5—11
Reference samples, dried bloodstains      6
Reference samples, mitochondrial DNA primers      195 195f
Relative fluorescent units (RFUs)      159
Reports, quality assurance standards      240
Restriction digestion, EcoRI      58f
Restriction digestion, HaeIII      58f
Restriction fragment-length, polymorphism (RFLP) typing, body fluid stains      59
Restriction fragment-length, polymorphism (RFLP) typing, effectiveness      59
Restriction fragment-length, polymorphism (RFLP) typing, immigration      59
Restriction fragment-length, polymorphism (RFLP) typing, paternity      59 60f
Restriction fragment-length, polymorphism (RFLP) typing, sexual assault      61f
Restriction fragment-length, polymorphism (RFLP), chemiluminescent detection      See Chemiluminescent detection restriction fragment-length polymorphism
Restriction fragment-length, polymorphism (RFLP), defined      230
Restriction fragment-length, polymorphism variable number tandem repeat loci (RFLP/ VNTR)      57—91
Restriction fragment-length, polymorphism variable number tandem repeat loci (RFLP/ VNTR), analytical gel      72—74
Restriction fragment-length, polymorphism variable number tandem repeat loci (RFLP/ VNTR), autoradiography      65—66 67f 81—82
Restriction fragment-length, polymorphism variable number tandem repeat loci (RFLP/ VNTR), blot stripping      82—83 83f
Restriction fragment-length, polymorphism variable number tandem repeat loci (RFLP/ VNTR), chemiluminescence      66—68 67f 84—91
Restriction fragment-length, polymorphism variable number tandem repeat loci (RFLP/ VNTR), DNA probe, commercially available      78
Restriction fragment-length, polymorphism variable number tandem repeat loci (RFLP/ VNTR), DNA probe, radiolabeling      76—78 77f
Restriction fragment-length, polymorphism variable number tandem repeat loci (RFLP/ VNTR), electrophoresis      60
Restriction fragment-length, polymorphism variable number tandem repeat loci (RFLP/ VNTR), hybridization      64—65 79
Restriction fragment-length, polymorphism variable number tandem repeat loci (RFLP/ VNTR), liquid blood      69—70
Restriction fragment-length, polymorphism variable number tandem repeat loci (RFLP/ VNTR), liquid, HaeIII      69—70
Restriction fragment-length, polymorphism variable number tandem repeat loci (RFLP/ VNTR), membranes      62
Restriction fragment-length, polymorphism variable number tandem repeat loci (RFLP/ VNTR), post-hybridization washes      65 81
Restriction fragment-length, polymorphism variable number tandem repeat loci (RFLP/ VNTR), probe      63—64 64f
Restriction fragment-length, polymorphism variable number tandem repeat loci (RFLP/ VNTR), Southern blotting      61—62 74—76
Restriction fragment-length, polymorphism variable number tandem repeat loci (RFLP/ VNTR), stains      70
Restriction fragment-length, polymorphism variable number tandem repeat loci (RFLP/ VNTR), stripping      68
Restriction fragment-length, polymorphism variable number tandem repeat loci (RFLP/ VNTR), test gel      71—72
Reverse dot blot format      101 103f
Reverse dot blot format, probes      104f
Review, defined      231
Review, quality assurance standards      240
RFLP      See Restriction fragment-length polymorphism
RFUs      159
Saliva, DNA extraction, Chelex method      37
Saliva, DNA extraction, micron 100 washing      20—22
Saliva, samples, collection      5
Salting out method, DNA extraction, whole blood      41
Sanger sequencing reaction      192
Second agency defined      231
Semen stains, DNA extraction, Chelex method      36—37
Semen stains, DNA extraction, micron 100 washing      22—23
Sequence Navigator Software      217—224 217f—223f
Sexual assault, Profiler Plus      186f
Sexual assault, RFLP      61f
Short tandem repeat loci (STR)      135—140
Short tandem repeat loci (STR), advantages      135—136
Short tandem repeat loci (STR), allele designation, STaR CALL      154—155
Short tandem repeat loci (STR), amplified      138 140—141
Short tandem repeat loci (STR), electrophoresis      144
Short tandem repeat loci (STR), electrophoresis, sample preparation      143
Short tandem repeat loci (STR), multicolor gel analysis, FMBIO analysis software 8.0      148—154
Short tandem repeat loci (STR), off-ladder variants      139f
Short tandem repeat loci (STR), polyacrylamide gel analysis FMBIO II      144—148
Short tandem repeat loci (STR), polyacrylamide gel, preparation      142—143
Short tandem repeat loci (STR), typing, capillary electrophoresis      166—167
Sieving medium, capillary electrophoresis      158
Silver stains vs. ethidium bromide      121—122
Silver stains, polyacrylamide gels, AMP-FLP      130—131 130f
Silver-stained gels, AMP-FLP, photoduplication      131—132
Slab gel vs. capillary electrophoresis      157
Slide-mounted hair specimens, DNA extraction      25
Slot blot apparatus      51f
Slot blot hybridization, DNA quantity determination      46
Slot blot hybridization, DNA quantity determination, $^{32}P$ -labeled alphoid probe      50—53
Slot blot hybridization, DNA quantity determination, alkaline phosphatase (AP)-labeled probe      53—55
Slot blot hybridization, DNA quantity determination, chemiluminescence      53—55 55f
Soft tissues, DNA extraction      24—25
Southern blot apparatus, modified      63f
Southern blotting, chemiluminescent detection RFLPs      85—86
Southern blotting, variable number tandem repeat, loci, RFLP      61—62 74—76
Spectrophotometry, DNA quantity determination      45
Spectrophotometry, DNA quantity determination, liquid blood      46—47
Sperm cell, DNA extraction, alcohol precipitation method      18—20
SPEX 6700 Freezer/Mill, bone      28—29
SPEX 6700 Freezer/Mill, teeth      33—35
Stains      121—122
Stamps, DNA extraction      24
Stamps, DNA extraction, Chelex method      38
STaR CALL, allele designation      154—155
STR      See Short tandem repeat
Streptavidin-horseradish-peroxidase complex      103
Stripping blot, chemiluminescent detection RFLPs      90—91
Stripping blot, RFLP VNTR      82—83 83f
Stripping RFLP VNTR      68
Strips storage, PCR      114—115
Stutter bands      137 137f—139f
Subcontractor, defined      231
Sulfate-borate      121
supplies      269—292
Support personnel, requirements, quality assurance standards      234
Swab, buccal, C.E.P.      10f
Swab, buccal, foam applicator      11f
Swab, buccal, Gene Guard      10f
Swab, buccal, protocol      9—11
Swab, buccal, vaginal, micron 100 washing      22—23
System Gold programming, mitochondrial DNA (mtDNA), sequencing      200
TAE      120
Taq      96
TBE      120
Technical leader, requirements, quality assurance standards      232—233
Technical manager, defined      231
Technical review, defined      231
Technical Working Group on DNA, Analysis Methods (TWGDAM)      227
Technician, defined      231
Technician, requirements, quality assurance standards      234
Teeth, DNA extraction      31—35
Teeth, DNA extraction, Bessman Pulverizer      32—33
Teeth, DNA extraction, SPEX 6700 Freezer/Mill      33—35
Teeth, DNA extraction, with soft tissue present      31—32

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