Nonradioactive in Situ Hybridization Application Manual :: Электронная библиотека попечительского совета мехмата МГУ

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Nonradioactive in Situ Hybridization Application Manual

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Название: Nonradioactive in Situ Hybridization Application Manual

Язык:

Рубрика: Природа/

Статус предметного указателя: Готов указатель с номерами страниц

ed2k: ed2k stats

Издание: second edition

Год издания: 1996

Количество страниц: 213

Добавлена в каталог: 16.11.2006

Операции: Положить на полку | Скопировать ссылку для форума | Скопировать ID

Предметный указатель

Paraffin embedded      19 127
Paraformaldehyde      19 127
Patel      162
PCR      13 72
PCR DIG Labeling Mix      36
PCR DIG Probe Synthesis Kit      36
PCR fluorescein labeling      39
PCR Fluorescein Labeling Mix      36
PCR labeling      36
PCR primers      13
Penetration of probes      14 20 30
Pepsin      20
Permeabilization      19
Peroxidase      22
Ph      28
Phase contrast      23
POD      22
Polylinker      44
Polylysine      19
Polymerase chain reaction      see “PCR”
Polymerization      149
Polytene chromosomes      97
Popp      68
Positive controls      131
Posthybridization washes      21
Prehybridization      20
Preparation of metaphase chromosomes      62 88 94 100
Preparation of slides      80
Pretreatment of sections      136
Pretreatment of slides      154
PRimed IN Situ Labeling      79; see also “PRINS”
Primer annealing      89
Primer elongation      82
Prins      79
PRINS and FISH      83
PRINS oligonucleotide primer      79
PRINS Reaction Set      79
Probe concentration      29
Probe length      29
Probe sequences, unique      36 63 101
Probes containing repetitive elements      63
Problems with radioactive probes      8
Procedures for ISH      58
Propidium iodide      22 90
Protease      20
Protein embedding layer      104
Proteinase K      123 136 166
Purification of labeled probe      41
Purification of template DNA      44
PVA      144
Rameaekers      100 110
Random primed labeling      13 34
Rate limiting step in hybridization      29
Rate of renaturation      29
Ratio-labeling      21
rDNA      169
Reactive oligonucleotide      14
Reanneal      30
Red fluorescence      21
Reflection-contrast microscopy      23
Repeated sequences      165
Repetitive DNA      30
Repetitive DNA probe      62 101
Rhodamine      6 12 21
Rhodamine-PRINS Reaction Set      79
RibosomalDNA      148
RNA complementary      13
RNA labeling      44
RNA polymerase      10 13 44
RNA probes      13 126 127
RNA transcript      44
RNA-RNA in situ hybridization      141 152
RNase      19 62 129 166
Rolighed      122
rRNA      148
Run-around transcripts      44
Run-off transcripts      44
Sagner      88 108
Salt concentration      14 28 30
Scheer      148
Schleifer      119
Schmidt      97
Schuetz      72
Sectioning      149
Segmentation gene      158
Seibl      79
Seminal vesicle secretion protein II      131
Sense RNA probe      128 137 150 153

Sensitivity      8 47
Short probes      30
Signal amplification      13
Silane-coated slides      126
Silver enhancement      150
Silver grains under darkfield microscopy      23
Simultaneous probe and target denaturation      101
Single color fluorescent detection      64
Single-stranded probes      30
Size of probe      13
Size of random primed labeled DNA fragments      34
Slide preparation      19
Small size      34
SP6      10
Speel      100 110
SSC      31
Stability influence of the hybridization, conditions on      14
Stability of ICC precipitate      110
Stability of labeled probes      49
Stability of probe-target interaction      14
Standard in situ hybridization conditions      31
Standard labeling reaction      34
Starry background fluorescence      71
Steric interaction between the hapten and anti-DIG antibody      10
Storage of DIG-labeled probes      49
Strength of hybridization bond      14
Streptavidin      11
Stringencies      21 30
Stringency washes      30
Substrates      22
Suchanek      136
Synthetic oligonucleotides      14
T3      10
T7      10
Tailing      47
Taq polymerase      10
Tautz      158
Temperature      28
Template amount, effect of      34
Terminal deoxynucleotidyl transferase      14
Tetramethylbenzidine      103
Tetramethylrhodamine-dUTP      41
Tetrasomy      67
Texas red      21
Thermal stability      14
Thermostable DNA polymerase      10
Tissue preparation1      23 127 136 148 154 165
TMB      103
Triple color FISH      65
Trisomy      67
Troubleshooting CGH experiments      77
Troubleshooting CGH experiments insufficient suppression of repetitive sequences      77
Troubleshooting CGH experiments non-homogeneous illumination of the optical field      77
Troubleshooting CGH experiments non-homogeneous staining patterns      77
Troubleshooting CGH experiments problems with chromosomal preparations      77
Troubleshooting CGH experiments problems with probes      77
Troubleshooting guide for general strong staining of chromosomes      71
Troubleshooting guide for hybridization signal fades      71
Troubleshooting guide for in situ hybridization      70
Troubleshooting guide for insufficient blocking      71
Troubleshooting guide for labeled probe molecules are too long      71
Troubleshooting guide for microscope      71
Troubleshooting guide for milky background fluorescence      71
Troubleshooting guide for modify chromosome denaturation      70
Troubleshooting guide for PRINS      84
Troubleshooting guide for PRINS signal is too strong      84
Troubleshooting guide for PRINS signal is weak or missing      84
Troubleshooting guide for probe labeling      70
Troubleshooting guide for size of labeled probe      70
Troubleshooting guide for starry background fluorescence      71
Tumor cell      113
Twelve color FISH      65
Types of nonradioactive hybridization methods      9
Unique probe sequences      36 63 101
Unwanted background signals      85
Van Oostveldt      165
Ward      68
Washes      21
Weisenberger      148
Whole blood      88
Whole mount FISH      165
Whole mount ISH, index of articles      61
Wiegant      62
Wienberg      67
X-GAL      112
Yield of DIG-labeled Nucleic Acids      51
YOYO-1      22
Zarda      119

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