 incubator 88
 DNA 63 74 101
 127
 28
, calculation 28
Acetylate 130
Acute lymphoblastic leukemia 67
Affinity cytochemistry 14
Alkali-labile 37
Alkaline denaturations 20
Alkaline phosphatase 10 22 104 112
Amann 119
AMCA 10 21
AMCA-dUTP 41
Amount of labeled RNA transcript 44 45
Amount of nonradioactively labeled RNA probe 13
Amplified chromosome sequences 71 74
Aneuploidies 88
Anti-digoxigenin (anti-DIG) 10
Anti-digoxigenin (anti-DIG) -AMCA conjugate 90
Anti-digoxigenin (anti-DIG) -fluorescein conjugate 90
Anti-digoxigenin (anti-DIG) -fluorophore conjugate 90
Anti-digoxigenin (anti-DIG) -rhodamine conjugate 90
Antibody conjugates 12
Antifading agent 22 83 90
Antisense RNA probe 128 132 153 156
APAAP 138
APase 104 112
Aqueous mounting 130
Arnold 67
Assmann 152
ATPase 68
Autolysis 142
Avidin 11
Background 21
Background fluorescence 71
Bacterial cells 119
Base mismatch 30
Bauwens 165
BCIG 111 112
BCIP 22 103 110 129 143
Bentz 67 72
Beta-GAL 112
Bettag 94
Biotin labeling 11
Biotin RNA Labeling Mix 44
Biotin-ddUTP 46
Biotin-dUTP 47
Biotin-High Prime 34
Biotin-labeled probe 62
Bladder tumor 105
Blocking reagent 21
Blocking, insufficient 71
Blue fluorescence 21
Bouin's fixative 19
BrdU labeling 111
Breitschopf 136
Brightfield microscopy 23 110
Cabot 67
Cacodylate 46 47
Calculate formamide concentration 130
CCD 23 68
Celeda 94
Cell fixation 119
Cell preparation 116
Cell smears 119
Central cassette, DOP-PCR primer 72
Central nervous system 75
Cervix 72 74
CGH, insufficient quality of 77
Charged coupled device 23 68;
Chironomus 98
Chromosomal gains and losses 72
Chromosomal imbalances 72 74 78
Chromosomal in situ hybridization 62
Chromosomal in situ suppression 67
Chromosomal spreads 20 89
Chromosome 20
Chromosome medium 88
Chronic myeloid leukemia 66
Circular DNA 34
CISS 67
Colcemid 88
Collagenase 20
Colloidal gold 10
Colorimetric detection methods 21
Colors of fluorophores 21
Colors of immunocytochemical precipitates 103
Combinatorial labeling 21
Combined DNA in situ hybridization and immunocytochemistry 110
Comparative genomic hybridization 72
Competition in situ hybridization 30
Competitive hybridization 63
Competitor DNA 20 30
Complexity of probe 29
Condylomatous lesion 124
Confocal fluorescence microscopy 169
Confocal laser scanning microscopy 23
Controls, ISH 131
Conventional fluorescence microscopy 169
Cosmid probes 63
Coumann 21
Counterstain 22 83 90 130
Coverslip, cell preparations on 111
Cremer 68 94
cRNA probes 136 152
Crosslinking of proteins 19
Cryostat sections 19
Cultured cells 100 111 116
Cy3 21
Cycles, PCR 13
DAB 22 23
DAPI 22
Darkfield microscopy 23
De Block 141
de Jong 152
Debrouwer 141
Deionized formamide 71
Denaturation of polytene chromosomes 97
Denaturation of probe and target 20
Denhardt's reagent 31
DEPC 128
Detection of BrdU 113
Detection of proteins 138
Detergent 19
Dextran sulfate 29
Diaminobenzidine see “DAB”
Diethylpyrocarbonate see “DEPC”
DIG Oligonucleotide 3'-End Labeling Kit 46
DIG Oligonucleotide Tailing Kit 47
DIG RNA Labeling Mix 44
DIG, detection of 10 22
DIG-ddUTP 46
DIG-dUTP 47
DIG-High Prime 34
DIG-labeled DNA probe 62 97
DIG-labeled nucleotides 10
DIG-labeled RNA probes 127 136
DIG-labeled rRNA probes 148
DIG-NHS ester 10 14
DIG-UTP/dUTP/ddUTP 10
Digesting protein 20
Digital imaging 23
Digitalis 10
Digoxigenin see “DIG”
Dijkman 152
Direct detection of ISH 9
Divalent cations 28
DNA polymerase 10
DNA-DNA hybrids 19
| DNA-RNA hybrids 14
DNase I 13
Doehner 67
DOP-PCR 72
DOP-PCR primer 72
Double color FISH 65
Double-stranded probes 30
Drosophila 158
duplex 29
Effect of template amount 34
Efficiency of labeling 51
Electron microscopy 23
Embedding 113 149
Emission wavelength 23
Endogenous enzyme inactivation Endogenous RNA 19
Enzymatic labeling 11
enzyme 22
Enzyme precipitation reactions 110
Enzyme-based cytochemical detection 100
Even-skipped transcripts 163
Exponential amplification 72
Extraction of proteins 19
Fading of hybridization signal 70
Fast Red TR 22 104 108 110
Fischer 148
FISH 21 94
fixation 19
Flow cytometry 24
Flow diagram for in situ hybridization 24
Fluorescein 12
Fluorescein RNA Labeling Mix 44
Fluorescein-ddUTP 46
Fluorescein-dUTP 12 47
Fluorescein-High Prime 34
Fluorescein-labeled nucleotides 10
Fluorescein-labeled probes 65
Fluorescence 21
fluorescence in situ hybridization 94
Fluorescence microscopy 111
Fluorescent detection of alkaline phosphatase 21
Fluorescent ICC 110
Fluorochrome-labeled nucleotides 12
Fluorochromes 21
Formaldehyde 19
Formalin fixation 19
Formamide 29
Fragment length 29
Freeze-dried 19
Frozen sections 127 154
GC content 28
GC pairs 28
Gelatin capsules 149
Gelatin-coated slides 126
Genetic linkage maps 88
Genomic DNA 28
Glutaraldehyde fixation 141
Glutaraldehyde-activated gelatin chrome aluminum slides 19
Glycerol-para-phenylenediamine-mixture 98
Goodman 162
Green fluorescence 21
Guidelines for in situ hybridization 18
HCl 19
Heat denaturation of probe and target 20
Heat-denatured DNA for random primed labeling 34
Heterogeneous population of probe 13
High Prime 34
High Pure PCR Product Purification Kit 50
High stringency 30
Highly repetitive DNA 101
HNPP 22 108
HNPP Fluorescent Detection Set 108
Homogeneous labeling methods for DNA 13
Homogeneous labeling methods for RNA 13
Hopman 100 110
Horseradish peroxidase 103
Human chromosome-specific satellite DNA probes 88
Human chromosomes in suspension 94
Hunchback 158
hybridization 30
Hybridization buffer 101
Hybridization temperature 28
Hybridization variables 29
Hydrating 29
Hydrolyze probe 142
ICC 110
Immobilized nucleic acids 28
Immunocytochemical detection schemes 168
Immunocytochemistry 21 138
In situ hybridization to mRNA 116
In situ PCR 13
In situ renaturation of target DNA 14
In vitro transcription 44
Indirect (immunological) detection 9
Indirect detection 79
Infiltrate with embedding medium 149
Infrared dye 21
Interphase nuclei 66
ISH protocol, general outline of 18
ISH to cells, index of articles 60
ISH to tissues, index of articles 60
ISH to whole chromosomes, index of articles 59
Isolated metaphase chromosomes 94
Joos 70 72
Ki67 antigen 113
Kinetics 28
Klenow enzyme 13
Komminoth 126
Labeling density 34
Labeling efficiency 51
Labeling synthetic oligonucleotides enzymatically 14
Large DNA probes 31
Length and composition of the tail 47
Length of amplified probe 13
Length of labeled fragments 13
Lichter 66 67 70 72
Lindeberg 122
Linear DNA 34
Linearize 44
Lipid membrane components 19
Long probes 31
Lower stringency 30
Lung tumor cell line 113
Lymphocytes 100
Mathieu 66 67
Melting point 28
Melting temperature 28
Mentzel 152
Metaphase chromosome spreads 62 88 94 100
Methanol-acetic acid fixation 89
Milky background fluorescence 71
Modified nucleotide 34
Modified oligonucleotides 9
Monovalent cations 28
mRNA 126 136
Multicolor FISH 65
Multiple simultaneous hybridizations 12
Multiple target, multicolor detection 101
Naphthol-ASMX-phosphate 104 112
NBT 10 22 104 110 129 143
Negative controls 73 131
Nick translation 13 40 42
Nitro-blue tetrazolium 10 22 104 110 129 143
Non-homogeneous staining patterns 870
Oligonucleotide 31
Oligonucleotide 3'-end labeling 46
Oligonucleotide containing functional groups 9
Oligonucleotide hybridization 30
Oligonucleotide probes 130
Oligonucleotide tailing 47
PAP 139
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