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Nonradioactive in Situ Hybridization Application Manual
Nonradioactive in Situ Hybridization Application Manual



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Название: Nonradioactive in Situ Hybridization Application Manual

Язык: en

Рубрика: Природа/

Статус предметного указателя: Готов указатель с номерами страниц

ed2k: ed2k stats

Издание: second edition

Год издания: 1996

Количество страниц: 213

Добавлена в каталог: 16.11.2006

Операции: Положить на полку | Скопировать ссылку для форума | Скопировать ID
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Предметный указатель
$\mathrm{CO}_2$ incubator      88
$\mathrm{C}_0\mathrm{t}\textrm{-1}$ DNA      63 74 101
$\mathrm{ddH}_2\mathrm{O}$      127
$\mathrm{T}_\mathrm{m}$      28
$\mathrm{T}_\mathrm{m}$, calculation      28
Acetylate      130
Acute lymphoblastic leukemia      67
Affinity cytochemistry      14
Alkali-labile      37
Alkaline denaturations      20
Alkaline phosphatase      10 22 104 112
Amann      119
AMCA      10 21
AMCA-dUTP      41
Amount of labeled RNA transcript      44 45
Amount of nonradioactively labeled RNA probe      13
Amplified chromosome sequences      71 74
Aneuploidies      88
Anti-digoxigenin (anti-DIG)      10
Anti-digoxigenin (anti-DIG) -AMCA conjugate      90
Anti-digoxigenin (anti-DIG) -fluorescein conjugate      90
Anti-digoxigenin (anti-DIG) -fluorophore conjugate      90
Anti-digoxigenin (anti-DIG) -rhodamine conjugate      90
Antibody conjugates      12
Antifading agent      22 83 90
Antisense RNA probe      128 132 153 156
APAAP      138
APase      104 112
Aqueous mounting      130
Arnold      67
Assmann      152
ATPase      68
Autolysis      142
Avidin      11
Background      21
Background fluorescence      71
Bacterial cells      119
Base mismatch      30
Bauwens      165
BCIG      111 112
BCIP      22 103 110 129 143
Bentz      67 72
Beta-GAL      112
Bettag      94
Biotin labeling      11
Biotin RNA Labeling Mix      44
Biotin-ddUTP      46
Biotin-dUTP      47
Biotin-High Prime      34
Biotin-labeled probe      62
Bladder tumor      105
Blocking reagent      21
Blocking, insufficient      71
Blue fluorescence      21
Bouin's fixative      19
BrdU labeling      111
Breitschopf      136
Brightfield microscopy      23 110
Cabot      67
Cacodylate      46 47
Calculate formamide concentration      130
CCD      23 68
Celeda      94
Cell fixation      119
Cell preparation      116
Cell smears      119
Central cassette, DOP-PCR primer      72
Central nervous system      75
Cervix      72 74
CGH, insufficient quality of      77
Charged coupled device      23 68;
Chironomus      98
Chromosomal gains and losses      72
Chromosomal imbalances      72 74 78
Chromosomal in situ hybridization      62
Chromosomal in situ suppression      67
Chromosomal spreads      20 89
Chromosome      20
Chromosome medium      88
Chronic myeloid leukemia      66
Circular DNA      34
CISS      67
Colcemid      88
Collagenase      20
Colloidal gold      10
Colorimetric detection methods      21
Colors of fluorophores      21
Colors of immunocytochemical precipitates      103
Combinatorial labeling      21
Combined DNA in situ hybridization and immunocytochemistry      110
Comparative genomic hybridization      72
Competition in situ hybridization      30
Competitive hybridization      63
Competitor DNA      20 30
Complexity of probe      29
Condylomatous lesion      124
Confocal fluorescence microscopy      169
Confocal laser scanning microscopy      23
Controls, ISH      131
Conventional fluorescence microscopy      169
Cosmid probes      63
Coumann      21
Counterstain      22 83 90 130
Coverslip, cell preparations on      111
Cremer      68 94
cRNA probes      136 152
Crosslinking of proteins      19
Cryostat sections      19
Cultured cells      100 111 116
Cy3      21
Cycles, PCR      13
DAB      22 23
DAPI      22
Darkfield microscopy      23
De Block      141
de Jong      152
Debrouwer      141
Deionized formamide      71
Denaturation of polytene chromosomes      97
Denaturation of probe and target      20
Denhardt's reagent      31
DEPC      128
Detection of BrdU      113
Detection of proteins      138
Detergent      19
Dextran sulfate      29
Diaminobenzidine      see “DAB”
Diethylpyrocarbonate      see “DEPC”
DIG Oligonucleotide 3'-End Labeling Kit      46
DIG Oligonucleotide Tailing Kit      47
DIG RNA Labeling Mix      44
DIG, detection of      10 22
DIG-ddUTP      46
DIG-dUTP      47
DIG-High Prime      34
DIG-labeled DNA probe      62 97
DIG-labeled nucleotides      10
DIG-labeled RNA probes      127 136
DIG-labeled rRNA probes      148
DIG-NHS ester      10 14
DIG-UTP/dUTP/ddUTP      10
Digesting protein      20
Digital imaging      23
Digitalis      10
Digoxigenin      see “DIG”
Dijkman      152
Direct detection of ISH      9
Divalent cations      28
DNA polymerase      10
DNA-DNA hybrids      19
DNA-RNA hybrids      14
DNase I      13
Doehner      67
DOP-PCR      72
DOP-PCR primer      72
Double color FISH      65
Double-stranded probes      30
Drosophila      158
duplex      29
Effect of template amount      34
Efficiency of labeling      51
Electron microscopy      23
Embedding      113 149
Emission wavelength      23
Endogenous enzyme inactivation Endogenous RNA      19
Enzymatic labeling      11
enzyme      22
Enzyme precipitation reactions      110
Enzyme-based cytochemical detection      100
Even-skipped transcripts      163
Exponential amplification      72
Extraction of proteins      19
Fading of hybridization signal      70
Fast Red TR      22 104 108 110
Fischer      148
FISH      21 94
fixation      19
Flow cytometry      24
Flow diagram for in situ hybridization      24
Fluorescein      12
Fluorescein RNA Labeling Mix      44
Fluorescein-ddUTP      46
Fluorescein-dUTP      12 47
Fluorescein-High Prime      34
Fluorescein-labeled nucleotides      10
Fluorescein-labeled probes      65
Fluorescence      21
fluorescence in situ hybridization      94
Fluorescence microscopy      111
Fluorescent detection of alkaline phosphatase      21
Fluorescent ICC      110
Fluorochrome-labeled nucleotides      12
Fluorochromes      21
Formaldehyde      19
Formalin fixation      19
Formamide      29
Fragment length      29
Freeze-dried      19
Frozen sections      127 154
GC content      28
GC pairs      28
Gelatin capsules      149
Gelatin-coated slides      126
Genetic linkage maps      88
Genomic DNA      28
Glutaraldehyde fixation      141
Glutaraldehyde-activated gelatin chrome aluminum slides      19
Glycerol-para-phenylenediamine-mixture      98
Goodman      162
Green fluorescence      21
Guidelines for in situ hybridization      18
HCl      19
Heat denaturation of probe and target      20
Heat-denatured DNA for random primed labeling      34
Heterogeneous population of probe      13
High Prime      34
High Pure PCR Product Purification Kit      50
High stringency      30
Highly repetitive DNA      101
HNPP      22 108
HNPP Fluorescent Detection Set      108
Homogeneous labeling methods for DNA      13
Homogeneous labeling methods for RNA      13
Hopman      100 110
Horseradish peroxidase      103
Human chromosome-specific satellite DNA probes      88
Human chromosomes in suspension      94
Hunchback      158
hybridization      30
Hybridization buffer      101
Hybridization temperature      28
Hybridization variables      29
Hydrating      29
Hydrolyze probe      142
ICC      110
Immobilized nucleic acids      28
Immunocytochemical detection schemes      168
Immunocytochemistry      21 138
In situ hybridization to mRNA      116
In situ PCR      13
In situ renaturation of target DNA      14
In vitro transcription      44
Indirect (immunological) detection      9
Indirect detection      79
Infiltrate with embedding medium      149
Infrared dye      21
Interphase nuclei      66
ISH protocol, general outline of      18
ISH to cells, index of articles      60
ISH to tissues, index of articles      60
ISH to whole chromosomes, index of articles      59
Isolated metaphase chromosomes      94
Joos      70 72
Ki67 antigen      113
Kinetics      28
Klenow enzyme      13
Komminoth      126
Labeling density      34
Labeling efficiency      51
Labeling synthetic oligonucleotides enzymatically      14
Large DNA probes      31
Length and composition of the tail      47
Length of amplified probe      13
Length of labeled fragments      13
Lichter      66 67 70 72
Lindeberg      122
Linear DNA      34
Linearize      44
Lipid membrane components      19
Long probes      31
Lower stringency      30
Lung tumor cell line      113
Lymphocytes      100
Mathieu      66 67
Melting point      28
Melting temperature      28
Mentzel      152
Metaphase chromosome spreads      62 88 94 100
Methanol-acetic acid fixation      89
Milky background fluorescence      71
Modified nucleotide      34
Modified oligonucleotides      9
Monovalent cations      28
mRNA      126 136
Multicolor FISH      65
Multiple simultaneous hybridizations      12
Multiple target, multicolor detection      101
Naphthol-ASMX-phosphate      104 112
NBT      10 22 104 110 129 143
Negative controls      73 131
Nick translation      13 40 42
Nitro-blue tetrazolium      10 22 104 110 129 143
Non-homogeneous staining patterns      870
Oligonucleotide      31
Oligonucleotide 3'-end labeling      46
Oligonucleotide containing functional groups      9
Oligonucleotide hybridization      30
Oligonucleotide probes      130
Oligonucleotide tailing      47
PAP      139
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