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| Ïîèñê ïî óêàçàòåëÿì |
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| Bagasra O., Hansen J. — In Situ PCR Techniques |
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| Ïðåäìåòíûé óêàçàòåëü |
Acetate buffer solution 99
AES silane, glass slide preparation 30—31
Ampli-Taq Gold, in situ PCR, hot start technique 57—58
Amplicon length, primer design 13
Amplicons: in situ PCR amplification, immunohistochemistry applications 63
Amplicons: sequencing and cloning 64—66
Amplification procedures: amplicon recovery for sequencing and cloning 64—66
Amplification procedures: chromosomal applications 68—72
Amplification procedures: conventional sealing technologies 49—51
Amplification procedures: conventional sealing technologies, Self-Seal 51—53
Amplification procedures: cover glass attachment with nail polish 54—55
Amplification procedures: electron microscopy 61—62
Amplification procedures: gene therapy regimes 66—68
Amplification procedures: hot start technique 56—58
Amplification procedures: immunohistochemistry 62—63
Amplification procedures: in situ PCR 49—60
Amplification procedures: multiple signals, multiple labels in individual cells 64
Amplification procedures: one-step reverse transcription 53—54
Amplification procedures: optimization with Self-Seal technology 51—53
Amplification procedures: plant tissues 73—75
Amplification procedures: protocols 49—54
Amplification procedures: rare event detection 119—125
Amplification procedures: sealing and tissue attachment alternatives 55—56
Amplification procedures: solutions for 98—99
Amplification procedures: thermal cyclers 58—60
AMVRT (Avian myeloblastosis virus reverse transcriptase) enzyme: in situ PCR 46—47
AMVRT (Avian myeloblastosis virus reverse transcriptase) enzyme: primers for 49
AMVRT (Avian myeloblastosis virus reverse transcriptase) enzyme: temperature calculations 16
Annealing: chain reaction 4
Annealing: DNA primer design 10
Annealing: in situ PCR, hot start technique 56—58
Annealing: solution-based PCR 24
Annealing: temperature calculations 16—18
Annealing: touchdown protocols for primers and probes 16—18
Archival tissue, preparation 36
Bone fragments, DNA in 22
Bone marrow cells, amplification techniques 120—123
CD34 cells, in situ PCR techniques, gene therapy regimes 67—68
cDNA: in situ PCR: heat-stabilization treatment 38
cDNA: in situ PCR: primers for RT reaction 48—49
cDNA: in situ PCR: reverse transcriptase 8—9
cDNA: RNA target primer design 11—12
cDNA: solution-based RNA PCR 25—26
Cell cultures: in situ PCR techniques, chromosome spreads 70—71
Cell cultures: on glass slides 32
Cell surface antigens, in situ PCR amplification 63
Cell suspensions: DNA/RNA extraction 20—21
Cell suspensions: tissue preparation 31
Cellular antigens, in situ PCR amplification 63
Chain reaction mechanism 3—7
Chain reaction mechanism, annealing 4
Chain reaction mechanism, denaturation 4
Chain reaction mechanism, extension temperature 5
Chain reaction mechanism, geometric amplification 6—7
Chain reaction mechanism, oligonucleotide primers 3—4
Chain reaction mechanism, thermal cycle 5—6
Chitin resistance, plant tissue in situ PCR 71—72
Chromosome amplification: in situ PCR techniques 68—72
Chromosome amplification: in situ PCR techniques, cell arrest at mitotic metaphase 71
Chromosome amplification: in situ PCR techniques, harvesting 71—72
Chromosome amplification: in situ PCR techniques, slide preparation 72
Chromosome amplification: in situ PCR techniques, spread protocols 69—70
Chromosome amplification: solutions for 99
Cloning, in situ PCR amplification, amplicon recovery 64—66
Color solution, formula for 99
Commercial primers, availability 12—13
Computer-assisted design (CAD), primer design 109—118
Control procedures, in situ polymerase chain reaction (PCR) 93—95
Coplin jars 97
Cover glasses, in situ PCR, nail polish attachment of 54—55
Degenerate primers, chain reaction 4
Denaturation, chain reaction 4
Deoxyribonucleoside triphosphates (dNTPs), polymerase chain reaction (PCR) mechanism 3
Detection systems, solution-based PCR and 26—28
DNA fingerprinting, polymerase chain reaction (PCR) development 2
DNA polymerase: polymerase chain reaction (PCR) mechanism 3
DNA polymerase: reverse transcriptase and 8—9
DNA: amplification, primer-probe designs 15—16
DNA: commercial extraction preparations 20
DNA: extraction protocols 20—22
DNA: in bone fragments or forensic material 22
DNA: in paraffin-embedded tissue 21—22
DNA: in situ PCR: heat-stabilization treatment 37—38
DNA: in situ PCR: multiple signals and individual cell labeling 64
DNA: in situ PCR: proteinase K digestion 40
DNA: in situ PCR: sealing protocols 49
DNA: reverse transcription 7—9
DNA: solution-based PCR and 23—24
DNA: target primer design 9—10
DNAase: DNA Engine 58
Dot blot testing, solution-based PCR probes and detection systems 26—28
Downstream (antisense) primer: in situ PCR, reverse transcriptase reaction 46—47
Downstream (antisense) primer: in situ PCR, RNA target primer design 10—12
Downstream (antisense) primer: in situ PCR, RNA targets 44—45
Downstream (antisense) primer: in situ PCR, RNAase-free, DNAase solution 45—46
Downstream (antisense) primer: in situ PCR, RT reactions 48—49
Downstream (antisense) primer: in situ PCR, treatment protocols 45—47
Downstream (antisense) primer: reverse transcription 13
Downstream (antisense) primer: solution-based RNA PCR 25—26
Electron microscopy, in situ PCR amplification 62—63
Enzyme digestion, plant tissue in situ PCR 74—75
Ethanol, paraffin-fixed tissue slides 32—33
Extension temperature, polymerase chain reaction (PCR) 5
Ficoll — Hypaque density gradient, cell suspension tissue preparation 31
Fixation techniques, in situ PCR 38—39
Forensic material, DNA in 22
Formalin fixation: archival tissue preparation 36
Formalin fixation: in situ PCR amplification, cellular antigens 63
Frame Seal gaskets, in situ PCR techniques 56—58
Frozen sections, preparation techniques 33—36
FTTC labeling, in situ PCR amplification, immunohistochemistry 62—63
Gel electrophoresis, solution-based PCR 24—25
GenBank: oligonucleotide primers 3—4
GenBank: sequence data 14—15
GenBank: sequence retrieval 109—111
Gene Cone sealing technology: amplicon recovery 66
Gene Cone sealing technology: in situ PCR techniques 55—56
Gene therapy, in situ PCR techniques 66—68
GeneReleaser 20
Geometric amplification, polymerase chain reaction (PCR) 6—7
Glass slides: AES silanation 30—31
Glass slides: cell cultures 32
Glass slides: in situ PCR, chromosome spreads 72
Glass slides: plant tissue in situ PCR 75
Glass slides: sealing technologies 29—30
Glass slides: sources 96—97
Glass staining dishes 97
Harvesting techniques, in situ PCR, chromosome spreads 71—72
Heat-stabilization treatment, in situ PCR 37—38
HLA typing, polymerase chain reaction (PCR) development 2
HLA-DQa genes, in situ PCR, validation and control procedures 94—95
Hot start technique, in situ PCR 56—58
Human immunodeficiency virus-1 (HIV-1), in situ PCR analysis: automated microscopic detection 125
Human immunodeficiency virus-1 (HIV-1), in situ PCR analysis: chromosome spreads 68—69
Human immunodeficiency virus-1 (HIV-1), in situ PCR analysis: gene therapy regimes 67—68
Human immunodeficiency virus-1 (HIV-1), in situ PCR analysis: rare event detection 124—125
Human immunodeficiency virus-1 (HIV-1), in situ PCR analysis: validation and control procedures 93—95
Human immunodeficiency virus-1 (HIV-1), in situ PCR analysis: viral burden quantitation 125
Hydrogen peroxide, PBS solution and 98
Immunocytochemistry (ICC), rare event detection 119
Immunohistochemical techniques: frozen tissue preparation 34
Immunohistochemical techniques: in situ PCR amplification 62—63
In situ hybridization (ISH): automated microscopic HIV-1 detection 125
In situ hybridization (ISH): buffer solution 99
In situ hybridization (ISH): comparison with other techniques 78—79
In situ hybridization (ISH): directly incorporated labeled nucleotides, posthybridization 92
In situ hybridization (ISH): empirical characteristics 79—80
In situ hybridization (ISH): in situ PCR protocols 80—84 86—92
In situ hybridization (ISH): in situ PCR protocols, alkaline-phosphatase color posthybridization 90—92
In situ hybridization (ISH): in situ PCR protocols, detection methods 83
| In situ hybridization (ISH): in situ PCR protocols, digoxigenin-labeled probe posthybridization 92
In situ hybridization (ISH): in situ PCR protocols, FITC posthybridization 88—89
In situ hybridization (ISH): in situ PCR protocols, general hybridization 86—87
In situ hybridization (ISH): in situ PCR protocols, hybridization controls 83—84
In situ hybridization (ISH): in situ PCR protocols, labeling 82—83
In situ hybridization (ISH): in situ PCR protocols, probe characteristics 80—81
In situ hybridization (ISH): in situ PCR protocols, synthetic oligo probe 81—82
In situ hybridization (ISH): in situ PCR protocols, time, temperature and concentration 82
In situ hybridization (ISH): oligonucleotide probe labeling 84—86
In situ hybridization (ISH): peroxidase-based color posthybridization 89—90
In situ hybridization (ISH): peroxidase-based color posthybridization,  probe posthybridization 88
In situ hybridization (ISH): radioactive probes vs. nonradioactive detection 79
In situ hybridization (ISH): rare event detection 119—120
In situ hybridization (ISH): sealing and attachment alternatives 55—56
In situ hybridization (ISH): Self-Seal technology 51—53
In situ hybridization (ISH): thermal cyclers 58—60
In situ hybridization (ISH): validation and controls 93—95
In situ polymerase chain reaction (PCR): amplicon recovery for sequencing and cloning 64—66
In situ polymerase chain reaction (PCR): amplification procedures 49—60
In situ polymerase chain reaction (PCR): chromosomal applications 68—72
In situ polymerase chain reaction (PCR): cover glass attachment with nail polish 54—55
In situ polymerase chain reaction (PCR): digestion optimization 43—44
In situ polymerase chain reaction (PCR): DNAase treatment: reverse transcriptase reaction 46—47
In situ polymerase chain reaction (PCR): DNAase treatment: RNA targets 44—45
In situ polymerase chain reaction (PCR): DNAase treatment: RNAase-free DNAase solutions 45—46
In situ polymerase chain reaction (PCR): electron microscopy 61—62
In situ polymerase chain reaction (PCR): gene therapy regimes 66—68
In situ polymerase chain reaction (PCR): hot start technique 56—58
In situ polymerase chain reaction (PCR): immunohistochemistry 62—63
In situ polymerase chain reaction (PCR): multiple signals, multiple labels in individual cells 64
In situ polymerase chain reaction (PCR): nonradioactive-labeled nucleotides 60
In situ polymerase chain reaction (PCR): one-step reverse transcription 53—54
In situ polymerase chain reaction (PCR): plant tissues 73—75
In situ polymerase chain reaction (PCR): protocols 37—43 49—50
In situ polymerase chain reaction (PCR): protocols, fixation and washes 38—39
In situ polymerase chain reaction (PCR): protocols, heat-stabilization treatment for DNA/RNA messages 37—38
In situ polymerase chain reaction (PCR): protocols, proteinase K treatment 39—43
In situ polymerase chain reaction (PCR): rare event detection 123—125
In situ polymerase chain reaction (PCR): reaction primers 48—49
In situ polymerase chain reaction (PCR): RT enzymes 47—48
In situ polymerase chain reaction (PCR): schematic overview 48
In situ reactions, annealing temperature calculations 17—18
Infectious disease, rare event detection 123—125
Liquid nitrogen, frozen tissue preparation 35
Methyl celluloacetate ether (MCA), plastic sections tissue preparation 33
Methyl methacrylate (MMA), plastic sections tissue preparation 33
MMLVRT (Moloney murine leukemia virus reverse transcriptase) enzyme in situ PCR 46—47
MMLVRT (Moloney murine leukemia virus reverse transcriptase) enzyme in situ PCR, primers for 49
Monoclonal antibodies, in situ PCR, hot start technique 57—58
Mononuclear cells, DNA/RNA extraction 20—21
mRNA: in situ PCR: amplification, multiple signals and individual cell labeling 64
mRNA: in situ PCR: heat-stabilization treatment 38
mRNA: in situ PCR: primers for RT reaction 48—49
mRNA: in situ PCR: proteinase Ê digestion 40
mRNA: RNA target primer design 11—12
mRNA: solution-based RNA PCR 25—26
Nail polish sealing technique 29—30
Nail polish sealing technique, in situ PCR: amplicon recovery 66
Nail polish sealing technique, in situ PCR: conventional protocols 50—51
Nail polish sealing technique, in situ PCR: cover glass attachment with 54—55
Nail polish, in situ PCR techniques, amplicon recovery 66
Nonradioactive nucleotides, in situ PCR 60
Nucleotides: DNA primer design 9—10
Nucleotides: in situ PCR 60
Nunc glass slides 32
Oligo d(T) primer: amplicon length 13
Oligo d(T) primer: in situ PCR: RT reaction 48—49
Oligo d(T) primer: in situ PCR: sequencing and cloning 64—66
Oligo d(T) primer: RNA target primer design 11
Oligo d(T) primer: solution-based RNA PCR 25—26
Oligo software 14
Oligonucleotide primers: chain reaction mechanism 3—4
Oligonucleotide primers: denaturation and annealing 4
Oligonucleotide primers: polymerase chain reaction (PCR) development 2
Oligonucleotides See also Primer design
Oncology research, amplification techniques 119—125
One-step reverse transcription, in situ PCR 53—54
Paraffin-fixed tissue 32—33
Paraffin-fixed tissue, DNA extraction 21—22
Paraffin-fixed tissue, frozen sections 33—34
Paraformaldehyde, 2% solution 97
PBS solution: 0.3% hydrogen peroxide 98
PBS solution: 10x solution, pH 7.2-7.4 97
PBS solution: 1x PBS 97
PBS solution: in situ PCR 38—39
Peripheral blood monocytes (PBMCs): DNA/RNA extraction 20—21
Peripheral blood monocytes (PBMCs): in situ PCR techniques chromosome spreads 70
Peripheral blood monocytes (PBMCs): in situ PCR techniques chromosome spreads, validation and control procedures 94—95
Permiofix: frozen tissue preparation 34
Permiofix: in situ PCR amplification, immunohistochemistry 63
Phytohemagglutinin (PHA), in situ PCR techniques, chromosome spreads 69
Plant tissue, in situ PCR 73—75
Plant tissue, protoplast protocol 74—75
Plastic sections tissue preparation 33
Polymerase chain reaction (PCR): annealing temperatures 16—18
Polymerase chain reaction (PCR): chain reaction mechanism 3—7
Polymerase chain reaction (PCR): chain reaction mechanism, denaturation and annealing 4
Polymerase chain reaction (PCR): chain reaction mechanism, extension 5
Polymerase chain reaction (PCR): chain reaction mechanism, geometric amplification 6—7
Polymerase chain reaction (PCR): chain reaction mechanism, oligonucleotide primers 3—4
Polymerase chain reaction (PCR): chain reaction mechanism,second thermal cycle 5—6
Polymerase chain reaction (PCR): historical background 1—2
Polymerase chain reaction (PCR): primer design 9—16
Polymerase chain reaction (PCR): primer design, amplicon length 13
Polymerase chain reaction (PCR): primer design, commercial available primer pairs 12—13
Polymerase chain reaction (PCR): primer design, DNA targets 9—10
Polymerase chain reaction (PCR): primer design, primer-probe design 15—16
Polymerase chain reaction (PCR): primer design, RNA targets 10—12
Polymerase chain reaction (PCR): primer design, sequence data sources 14—15
Polymerase chain reaction (PCR): reverse transcription 7—9
Polymerase chain reaction (PCR): touchdown protocols 16—18
Primer design: annealing temperature calculations 16—18
Primer design: computer-assisted design (CAD) 109—118
Primer design: in situ PCR, RT reactions 48—49
Primer design: optimization 117—118
Primer design: polymerase chain reaction (PCR) 9—16
Primer design: polymerase chain reaction (PCR), amplicon length 13
Primer design: polymerase chain reaction (PCR), DNA targets 9—10
Primer design: polymerase chain reaction (PCR), primer pairs, commercial availability 12—13
Primer design: polymerase chain reaction (PCR), primer-probe design 15—16
Primer design: polymerase chain reaction (PCR), RNA targets 10—12
Primer design: polymerase chain reaction (PCR), sequence data sources 14—15
Primer dimers, DNA primer design 10
Probe design: DNA amplification 15—16
Probe design: verification of 26—28
Probe design: verification of, dot blot testing 26—28
Probe design: verification of, Southern blot testing 28
Proteinase K, solution formula 98
Proteinase K: in situ PCR 39—40
Proteinase K: in situ PCR, alternative technique 40—43
Proteinase K: in situ PCR, amplicon recovery 65—66
Proteinase K: in situ PCR,optimization of 43
Proteinase K: solution formula 98
Proteins, in situ PCR amplification, multiple signals and individual cell labeling 64
Protoplasts, in situ PCR 74—75
Random primer, RNA target primer design 11
Rare event detection: amplification techniques 119—125
Rare event detection: infectious disease 123—125
Reagents: archival tissue preparation 36
Reagents: basic formulas 97—99
Reagents: DNA/RNA extraction, commercial preparations 20
Retroviruses, reverse transcriptase in 8
Reverse transcriptase (RT) enzymes See also specific RT enzymes
Reverse transcriptase (RT) enzymes in retroviruses 8
Reverse transcriptase (RT) enzymes in situ PCR 46—47
Reverse transcriptase (RT) enzymes in situ PCR, automated microscopic HIV-1 detection 125
Reverse transcriptase (RT) enzymes in situ PCR, primers for RT reactions 48—49
Reverse transcriptase (RT) enzymes in situ PCR, properties 47—48
Reverse transcriptase (RT) enzymes in situ PCR, rare event detection 119—125
Reverse transcriptase (RT) enzymes in situ PCR, validation and control procedures 95
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