Bagasra O., Hansen J. — In Situ PCR Techniques :: Электронная библиотека попечительского совета мехмата МГУ

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Bagasra O., Hansen J. — In Situ PCR Techniques

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Название: In Situ PCR Techniques

Авторы: Bagasra O., Hansen J.

Аннотация:

This book describes comprehensive step-by-step protocols for the delineation of genetic amplification and histological detection techniques. Each procedure has been tested and validated for its sensitivity, precision, and reproducibility, and the authors give advice on the design of primers for PCR applications and on optimizing these protocols for use with plant, insect, and prokaryotic cells.

Язык:

Рубрика: Технология/

Статус предметного указателя: Готов указатель с номерами страниц

ed2k: ed2k stats

Год издания: 1997

Количество страниц: 160

Добавлена в каталог: 12.11.2006

Операции: Положить на полку | Скопировать ссылку для форума | Скопировать ID

Предметный указатель

Reverse transcriptase (RT) enzymes, polymerase chain reaction (PCR) development      2
Reverse transcriptase (RT) enzymes, RNA target primer design      11
Reverse transcriptase (RT) enzymes, solution-based RNA PCR      25—26
Reverse transcription      7—9
Reverse transcription in situ PCR: one-step reverse transcription      53—54
Reverse transcription in situ PCR: primers for RT reaction      48—49
Reverse transcription, amplicon length      13
Reverse transcription, solution-based RNA PCR      25—26
Rhodamine, in situ PCR amplification: immunohistochemistry      62—63
Rhodamine, in situ PCR amplification: protein labeling      64
Ribonuclease H, reverse transcriptase and      8
Ribonuclease inhibitor, in situ PCR, RNAase-free, DNAase solution      45—46
RightPrimer software      14 112—115
RNA sequencing, polymerase chain reaction (PCR) development      2
RNA: commercial extraction preparations      20
RNA: extraction protocols      20—22
RNA: in situ PCR: DNAase treatment      44—45
RNA: in situ PCR: heat-stabilization treatment      37—38
RNA: reverse transcription      7—9
RNA: sequencing      2
RNA: solution-based PCR      25—26
RNA: target primer design      10—12
RNAsin, in situ PCR      47
Robocycler Gradient, annealing temperatures      17
rTth enzyme: in situ PCR: one-step reverse transcription      53—54
rTth enzyme: in situ PCR: RNA targets      44—45
rTth enzyme: in situ PCR: validation and control procedures      95
rTth enzyme: reverse transcription      9
rTth enzyme: RNA target primer design      12
Sealing protocols: glass slide preparation      29—30
Sealing protocols: in situ PCR      49—60
Sealing protocols: in situ PCR, alternative procedures      55—56
Sealing protocols: in situ PCR, conventional technologies      49—51
Sealing protocols: in situ PCR, cover glass attachment      54—55
Sealing protocols: in situ PCR, hot start technique      56—58
Sectioning techniques, frozen tissue preparation      35—36
Self-Seal optimization      51—53
Self-Seal reagent: archival tissue preparation      36
Self-Seal reagent: in situ PCR: amplicon recovery      66
Self-Seal reagent: in situ PCR: optimization      51—53
Sequencing techniques: GenBank sequence retrieval      109—111
Sequencing techniques: in situ PCR amplification, amplicon recovery      64—66
Sequencing techniques: primer design      14—15
Silanation: AES silanation      30—31
Silanation: glass slide preparation      29—30
Single-cell suspensions, DNA/RNA extraction      20—21
Software, primer design      14—15
Solution-based reactions: background      19—20

Solution-based reactions: commercial preparations for DNA/RNA extractions      20
Solution-based reactions: polymerase chain reaction (PCR) procedures      22—26
Solution-based reactions: polymerase chain reaction (PCR) procedures, DNA protocols      23—25
Solution-based reactions: polymerase chain reaction (PCR) procedures, RNA adaptations      25—26
Solution-based reactions: probe verification and detection systems      26—28
Solution-based reactions: probe verification and detection systems, dot blot testing      26—28
Solution-based reactions: probe verification and detection systems, Southern blot testing      28
Solution-based reactions: protocols      20—22
Solution-based reactions: protocols, bone fragments or forensic material      22
Solution-based reactions: protocols, cell suspensions      20—21
Solution-based reactions: protocols, paraffin-embedded tissue      21—22
Solutions, basic formulas      97—99
Southern blot testing, solution-based PCR probes and detection systems      28
Spindle inhibitors, in situ PCR techniques, chromosome spreads      69
SSC solution formulas      98
Staskus Laboratory, in situ PCR techniques      55—56
Sterile procedure, in situ PCR techniques, chromosome spreads      69—70
Streptavidin peroxidase, solution formula      99
Superscript II enzyme: in situ PCR      47
Superscript II enzyme: reverse transcription      9
Taq enzyme, in situ PCR: amplicon recovery      65—66
Taq enzyme, in situ PCR: validation and control procedures      94—95
TaqStart antibody, in situ PCR, hot start technique      57—58
Teflon coated slides: cell cultures on      32
Teflon coated slides: glass slide preparation      30
Teflon coated slides: paraffin-fixed tissue      32—33
Teflon coated slides: sources      96—97
Temperatures, touchdown protocols      16—18
Thermal cycle, polymerase chain reaction (PCR)      5—6
Thermal cycler: annealing temperatures      17—18
Thermal cycler: in situ PCR      58—60
Thermal cycler: in situ PCR, conventional sealing protocols      51
Thermal cycler: in situ PCR, slide sealing and tissue attachment      55—56
Thermal cycler: polymerase chain reaction (PCR)      5—6
Tissue preparation      31—36
Tissue preparation, archival tissue      36
Tissue preparation, cell cultures on slide      32
Tissue preparation, cell suspensions      31
Tissue preparation, frozen sections      33—36
Tissue preparation, in situ PCR, alternative attachment techniques      55—56
Tissue preparation, paraffin-fixed tissue      32—33
Tissue preparation, plastic sections      33
Tissue-Tek OCT, frozen tissue preparation      34
Touchdown protocols, primer/probe design      16—18
UHU Stic glue stick, in situ PCR      50 54—55
Validation procedures, in situ polymerase chain reaction (PCR)      93—95
[b]-Actin genes, in situ PCR, validation and control procedures      94—95
[b]-Globulin genes, in situ PCR, validation and control procedures      94—95

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