Àâòîðèçàöèÿ
Ïîèñê ïî óêàçàòåëÿì
Gooding K.M., Regnier F.E. — HPLC of biological macromolecules
Îáñóäèòå êíèãó íà íàó÷íîì ôîðóìå Íàøëè îïå÷àòêó?
Íàçâàíèå: HPLC of biological macromoleculesÀâòîðû: Gooding K.M., Regnier F.E. Àííîòàöèÿ: Completely revised and expanded to reflect the most recent innovations in HPLC from the past decade, this authoritative reference presents practical strategies for the evaluation and analysis of proteins, peptides, and polynucleotides and offers class-specific applications for the characterization and fractionation of biological macromolecules. Contains updated material on organic supports, size exclusion, ion exchange, hydrophobic interaction, and metal interaction chromatography! With contributions from leading experts in the field, the Second Edition of HPLC of Biological Macromolecules provides ·new chapters summarizing specialized detection systems ·current discussions on the chemical and biological properties of specific biomolecules ·detailed guidelines for the development of modern analytical techniques ·state-of-the-art exploration methods for complex mixtures ·successful implementation and investigation procedures With more than 2200 contemporary references—over 1000 more than the previous edition— HPLC of Biological Macromolecules, Second Edition is an essential source for biochemists and analytical biochemists, molecular and cell biologists, biophysicists, geneticists, chemical and biotechnological engineers, biochemical neuroendocrinologists, and upper-level undergraduate and graduate students in these disciplines.
ßçûê: Ðóáðèêà: Áèîëîãèÿ /Ñòàòóñ ïðåäìåòíîãî óêàçàòåëÿ: Ãîòîâ óêàçàòåëü ñ íîìåðàìè ñòðàíèö ed2k: ed2k stats Èçäàíèå: 2nd edition, revized and expandedÃîä èçäàíèÿ: 2002Êîëè÷åñòâî ñòðàíèö: 777Äîáàâëåíà â êàòàëîã: 29.11.2005Îïåðàöèè: Ïîëîæèòü íà ïîëêó |
Ñêîïèðîâàòü ññûëêó äëÿ ôîðóìà | Ñêîïèðîâàòü ID
Ïðåäìåòíûé óêàçàòåëü
Adsorption, isotherms 295—296 Affinity chromatography, antibodies 622—630 655—659 Affinity chromatography, biomimctic ligand 626—627 Affinity chromatography, ligands 334—336 Affinity chromatography, media 33—38 318 322—339 Affinity chromatography, membrane proteins 528—532 Affinity chromatography, protein A 622—625 Affinity chromatography, protein G 624—626 Affinity tags 249—250 Antibodies 607—613 Antibodies, immunodetection 653—659 Biological activity, preservation 285—289 Biological activity, protease inhibitors 286 Capacity factor (k'), effect of displacing species 137—144 Cooperativity 88—89 Data analysis, cereal proteins 562—566 Detergents 177—180 516—519 Detergents in MIC 270 Detergents, membrane proteins 385 Detergents, removal 387—389 Detergents, solubilization 384—387 Diffusion coefficient 63—64 Fermentation, strategies 289—291 Gel filtration chromatography See Size exclusion chromatography Glycoproteins 635—636 Gradient elution, computer simulation 426—430 Gradient elution, conformational effects 411—414 Gradient elution, molecular weight effect 410—411 Gradient elution, principles 398—410 Gradient elution, systematic development 414—426 Hemoglobin, 599—600 Hemoglobin, 601—602 Hemoglobin, 600—602 Hemoglobin, 603 Hemoglobin, 602 Hemoglobin, variants 589—591 Hemoglobin, variants, cation-exchange 592—598 Hemoglobin, variants, isoelectric focusing 592 Hemoglobin, variants, reversed-phase 593—599 Hydrodynamic volume 51—56 Hydrophilic interaction chromatography (HILIC), peptides 461—473 Hydrophobic interaction chromatography (HIC) 99—245 Hydrophobic interaction chromatography (HIC), antibodies 620—622 Hydrophobic interaction chromatography (HIC), applications 213—224 Hydrophobic interaction chromatography (HIC), comparison with RPC 116—117 201—202 Hydrophobic interaction chromatography (HIC), glycoproteins 643—646 Hydrophobic interaction chromatography (HIC), historical 103—110 Hydrophobic interaction chromatography (HIC), k' 137—144 Hydrophobic interaction chromatography (HIC), mechanism 125—129 Hydrophobic interaction chromatography (HIC), mobile phase 177—180 Hydrophobic interaction chromatography (HIC), mobile phase effects 129—137 Hydrophobic interaction chromatography (HIC), operation 207—209 Hydrophobic interaction chromatography (HIC), preparative 305 318—321 Hydrophobic interaction chromatography (HIC), sorbents 114—116 156—162 Hydrophobic interaction chromatography (HIC), strategy 197—201 Hydrophobic interaction chromatography (HIC), surface chemistry 163—168 Hydrophobic interactions, mechanisms 125—129 146—156 Hydrophobic interactions, models, preferential interaction 151—152 Hydrophobic interactions, models, solvophobic 146—151 Hydrophobic interactions, models, stoichiometric solvent displacement 152—155 Hydrophobic interactions, surface tension 146—151 177—180 Hydrophobic interactions, temperature, effects of 180—197 200—201 Hydrophobic interactions, thermodynamics 144—146 180—197 Hydroxyapatite chromatography (HAC), antibodies 619—620 Hydroxyapatite chromatography (HAC), glycoproteins 639—641 Immunodetection 653—688 Immunodetection, capillary electrophoretic 666—673 Immunodetection, enhancing sensitivity 673—682 Immunodetection, tandem chromatography 661—666 Ion-exchange chromatography (IEC) 81—98 Ion-exchange chromatography (IEC), antibodies 615—619 Ion-exchange chromatography (IEC), capacity 97—98 Ion-exchange chromatography (IEC), cereal proteins 553—556 Ion-exchange chromatography (IEC), cleaning columns 500 Ion-exchange chromatography (IEC), glycoproteins 638—639 Ion-exchange chromatography (IEC), hemoglobin 592—593 Ion-exchange chromatography (IEC), macromolecules, of 88—91 Ion-exchange chromatography (IEC), membrane proteins 521 525—527 536—537 Ion-exchange chromatography (IEC), mobile phase 95—97 Ion-exchange chromatography (IEC), peptides 452—461 Ion-exchange chromatography (IEC), preparative 303—317 Ion-exchange chromatography (IEC), resins 32—34 Ion-exchange chromatography (IEC), resolution 94—98 Ion-exchange chromatography (IEC), retention 91—94 Ion-exchange chromatography (IEC), sorbents 82—85 Loadability 2 Mass spectrometry (MS), electrospray ionization 693—697 Mass spectrometry (MS), fragmentation 704—709 Mass spectrometry (MS), glycoproteins 646—651 Mass spectrometry (MS), matrix-assisted laser desorption/ionization (MALDI) 697—704 Mass spectrometry (MS), methods 689—704 Mass spectrometry (MS), oligonucleotides 729—734 Mass spectrometry (MS), peptides 720—722 Mass spectrometry (MS), proteins after gel electrophoresis 722—729 Mass spectrometry (MS), recombinant proteins 711—720 Metal interaction chromatography (MIC) 247—280 Metal interaction chromatography (MIC), affinity tags 249—250 Metal interaction chromatography (MIC), amino acid specificity 249—252 Metal interaction chromatography (MIC), antibodies 627—628 Metal interaction chromatography (MIC), applications 272—276 Metal interaction chromatography (MIC), chelating ligates 254—257 Metal interaction chromatography (MIC), glycosylation, effect of 252 Metal interaction chromatography (MIC), metal ions 258—263 Metal interaction chromatography (MIC), mobile phase 263—271 Metal interaction chromatography (MIC), mobile phase, competitive displacers 269—270 Metal interaction chromatography (MIC), mobile phase, detergents 270 Metal interaction chromatography (MIC), mobile phase, organic solvents 270—271 Metal interaction chromatography (MIC), mobile phase, pH 267—269 Metal interaction chromatography (MIC), mobile phase, salt 263—267 Metal interaction chromatography (MIC), multimodal chromatography 276—277 Metal interaction chromatography (MIC), operation 271—272 Metal interaction chromatography (MIC), phosphate specificity 253 Metal interaction chromatography (MIC), stationary phase 253—263 Metal interaction chromatography (MIC), stationary phase, spacer arm 257 Metal interaction chromatography (MIC), stationary phase, support matrix 258 Mixed modality sorbents, hydrophobic 168—170 Mixed modality sorbents, metal ion interaction 276—277 Monolithic columns 11 43—45 Multistep HPLC 440—441 Oligonucleotidcs, MS of 729—734 p1 86—88 Peptide standards 441—442 Peptide standards, conformation 447—448 Peptide standards, IEC 454—461 Peptide standards, RPC 478—490 Peptide standards, SEC 445—446 Peptides 433—511 Peptides, characteristics 434—435 Peptides, column selection 437—441 Peptides, conformation 487—490 Peptides, detection 435—437 Peptides, HILIC/CEC 461—473 Peptides, IEC 452—461 Peptides, MS of 720—722 Peptides, prediction of retention 486—487 Peptides, RPC 473—500 Peptides, SEC 442—451 Peptides, troubleshooting 500—503 Photodiode array (PDA), conformational studies 755—767 Photodiode array (PDA), detector design 741—751 Photodiode array (PDA), flow cell design 745—751 Photodiode array (PDA), wavelength 752—755 Polymer support, agarose 21—22 Polymer support, cellulose 20—21 Polymer support, compressed gels 42 Polymer support, macroporous 22—25 Polymer support, membrane 40—42 Polymer support, monodisperse 38—39 Polymer support, monolith 43—45 Polymer support, pores 19—20 24 Polymer support, preparation 18—19 Polymer support, surface modification 26—27 Polymer support, surface modification, affinity 33—38 Polymer support, surface modification, hydrophobic 31—32 Polymer support, surface modification, ion-exchange 32—34 Polynucleotide, characteristics 282—285 Precipitation 377—378 389—390 Preparative chromatography 281—373 Preparative chromatography, adsorption isotherms 295—296 Preparative chromatography, analytical vs. 294—300 Preparative chromatography, cereal proteins 574 Preparative chromatography, continuous 359—369 Preparative chromatography, continuous, affinity belt 367—369 Preparative chromatography, continuous, affinity recycle extraction 366—367 Preparative chromatography, continuous, annular 363—365 Preparative chromatography, continuous, counter current 369 Preparative chromatography, continuous, membrane 365—366 Preparative chromatography, continuous, simulated moving bed 361—363 Preparative chromatography, equipment 298—300 Preparative chromatography, media 300—351 Preparative chromatography, media, adsorption 341 351 Preparative chromatography, media, affinity 318 322—339 Preparative chromatography, media, hydrophobic interaction 305 318—321 Preparative chromatography, media, ion-exchange 303—317 Preparative chromatography, media, modes 301—304 Preparative chromatography, media, particle size 352—353 Preparative chromatography, media, reversed phase 341 349—350 Preparative chromatography, media, size exclusion 336 342—348 Preparative chromatography, media, sorbents 301—302 Preparative chromatography, modes 296—298 Preparative chromatography, packing columns 357—359 Preparative chromatography, productivity 353—356 Preparative chromatography, scale-up 356—359 Pressure 183 Pressure in preparative chromatography 300—301 Protease inhibitors 286 Protein structure, characteristics 117—118 281—282 Protein structure, conformation by PDA 755—767 Protein structure, folding 191—197 Protein structure, solvent effects 118—120 Protein structure, spectroscopic elucidation 120—124 202—207 Protein structure, thermodynamics 180—197 Proteins, cereal 547—548 Proteins, cereal, characterization 566—570 Proteins, cereal, genetic studies 573—574 Proteins, cereal, quality 576—578 Proteins, cereal, varietal identification 570—573 Proteins, fusion 336 340 Proteins, membrane 513—516 Proteins, membrane, troubleshooting 532—535 Proteins, recombinant by MS 711—720 Proteins, therapeutic 292—294 Retention, models 146—155 Reversed-phase chromatography (RPC) 99—245 Reversed-phase chromatography (RPC), applications 209—212 222—224 Reversed-phase chromatography (RPC), cereal proteins 551—557 Reversed-phase chromatography (RPC), cleaning columns 501—502 Reversed-phase chromatography (RPC), comparison with HIC 116—117 201—202 Reversed-phase chromatography (RPC), glycoproteins 641—644 Reversed-phase chromatography (RPC), historical 103—110 Reversed-phase chromatography (RPC), ion pair agents 473—478 490—491 Reversed-phase chromatography (RPC), k' 137—144 Reversed-phase chromatography (RPC), mechanism 125—129 Reversed-phase chromatography (RPC), membrane proteins 530—534 537—540 Reversed-phase chromatography (RPC), mobile phase, effects 129—137 Reversed-phase chromatography (RPC), mobile phase, general 170—173 Reversed-phase chromatography (RPC), mobile phase, organic solvent 173—177 Reversed-phase chromatography (RPC), mobile phase, salts 177—180 Reversed-phase chromatography (RPC), operation 207—209 Reversed-phase chromatography (RPC), peptides 473—500 Reversed-phase chromatography (RPC), preparative 341 349—350 Reversed-phase chromatography (RPC), sorbents 110—114 156—162 Reversed-phase chromatography (RPC), strategy 197—201 Reversed-phase chromatography (RPC), surface chemistry 162—163 Sample preparation 375—395 Sample preparation, antibodies 613—614 Sample preparation, cereal proteins 548—551 560—561 Sample preparation, concentration 389—390 Sample preparation, desalting 391—392 Sample preparation, detergent removal 387—389 Sample preparation, extraction, liquid-liquid 378 Sample preparation, extraction, liquid-solid 378 Sample preparation, extraction, solid-phase (SPE) 379—384 Sample preparation, extraction, supercritical flow 378 Sample preparation, membrane proteins 519 Sample preparation, solubilization 384—387 Sample preparation, ultrafiltration 390 Silica, 6—7 Silica, calcination 5 Silica, pores 3—4 9 Silica, preparation 11—12 Silica, stability 10 Silica, structure 3 Silica, surface 4—7 Silica, surface area 9 Size exclusion chromatography (SEC) 49—79 Size exclusion chromatography (SEC), 50—52 Size exclusion chromatography (SEC), antibodies 614—616 Size exclusion chromatography (SEC), band spreading 61—64 Size exclusion chromatography (SEC), cereal proteins 552—554 556 Size exclusion chromatography (SEC), cleaning columns 500 Size exclusion chromatography (SEC), loading capacity 72 Size exclusion chromatography (SEC), membrane proteins 521—524 535—536 Size exclusion chromatography (SEC), methodology 69—72 Size exclusion chromatography (SEC), mobile phase 69—70 Size exclusion chromatography (SEC), nonideal 72—75 443—445 Size exclusion chromatography (SEC), peptides 442—451 Size exclusion chromatography (SEC), preparative 336 342—348 Size exclusion chromatography (SEC), resolution 56—64 Size exclusion chromatography (SEC), supports 64—68 Size exclusion chromatography (SEC), troubleshooting 77 Solid-phase extraction (SPE) 379—384 Solid-phase extraction (SPE), batch 379 Solid-phase extraction (SPE), cartridge 380—384 Solubilization 384—387 Spectroscopy, protein conformation 120—124 202—207 Surface tension 146—151 Temperature, effect of, cereal proteins 557—559 Temperature, effect of, hydrophobic interactions 180—197 200—201 Temperature, effect of, peptides 495—497 Titration curves 86—88 Void volume 50—51
Ðåêëàìà
|
|
Î ïðîåêòå