This book constitutes the refereed proceedings of the Third Australian Conference on Artificial Life, ACAL 2007, held in Gold Coast, Australia, in December 2007.

Research in Alife covers the main areas of biological behaviour as a metaphor for computational models, computational models that reproduce/duplicate a biological behaviour, and computational models to solve biological problems. Thus, Alife features analyses and understanding of life and nature and helps modeling biological systems or solving biological problems. The papers are organized in topical sections on heuristics, complex systems, evolution, biological systems, and networks.