Label-free biosensors use biological or chemical receptors to detect analytes
(molecules) in a sample. They give detailed information on the
binding selectivity, affinity, and, in many cases, the stoichiometry,
kinetics, and thermodynamics of an interaction. Although they can be
powerful tools in the hands of a skilled user, there is often a lack of
knowledge regarding the best way to utilize label-free assays to screen
for biologically active molecules and to accurately and precisely characterize
molecular recognition events.
This book reviews both established and newer label-free techniques.
It is intended to give both the expert user and the general reader insight
into the field from expert opinion leaders and practitioners of these
techniques. Chapters also contain worked examples that are written
to guide the reader through the basics of experimental design, setup,
assay development, and data analysis.