In Gene Therapy Protocols, leading researchers describe in detail all the essential molecular methods for developing gene transfer systems, along with the methods for introducing genes into specific tissue types either in vivo or ex vivo. These easily reproducible methods range from those for specific viral and nonviral delivery systems, to those concerned with gene delivery to particular tissues. Methods for applying specific therapeutic systems, such as ribozymes and tumor suppressor genes for the treatment of AIDS and cancer, are also included in this authoritative collection. Gene Therapy Protocols is the first major collection of the methods needed for successful gene delivery and subsequent in vivo gene expression, techniques at the center of the many and significant recent advances in the treatment of both genetic and acquired diseases. It will surely become today's indispensable standard reference source for all scientists working to realize the promises of gene therapy.