New waveguide effects are considered on the basis on various variants for self-focusing of the living cells of aquatic plant by laser beam irradiation. It is studying the process of forming of the 'living multy-mode fiber-optic' into the road of laser's beam in water with motion of green living cells. Analysis gives that the wide set of different profiles of the refractive index in this 'living optical fiber could be easy produced depending the frequency of applied lasers. Optical waveguide approach gives high sensitivity in living layer readout and control, this waveguides could be apply to environmental processing or for a cells studying, also different medical application could be realized according described mechanism.