Translated from the French edition of 1999 (de Hoffmann teaches at the Catholic University, Louvain; Stroobant is with the Ludwig Institute for Cancer Research), the second edition features an overall revision, new references to websites, and expanded coverage of biological applications and new techniques. Each chapter covers the history of study and technique of the topic, with an extensive list of references provided. Study questions and their answers are included, making this book appropriate for advanced undergraduate courses. Among the topics covered are ion sources, mass analyzers, tandem mass spectrometry, coupling, fragmentation reactions, and analysis of biomolecules.