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Bidlingmeyer B.A. — Practical HPLC Methodology and Applications
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Íàçâàíèå: Practical HPLC Methodology and Applications
Àâòîð: Bidlingmeyer B.A.
Àííîòàöèÿ: Of related interest… Trace and Ultratrace Analysis by HPLC Satinder Ahuja Written by a leading scientist in the field, this monograph provides the first definitive and technically up-to-date treatment of the theory, equipment, and applications of chemistry’s most powerful reliable analytical technique. Coverage includes an encyclopedic compendium of common substances that require trace and ultratrace analysis, and features clear discussion of such important topics as considerations for HPLC equipment, sensitive detectors, sample preparation, method development, selectivity and computer-based optimizations, optimizing detectability, and much more. 1991 (0 471-51419-5) 432 pp. High Performance Liquid Chromatography in Biotechnology Edited by William S. Hancock Analytical chemists, biochemists, and chemical engineers will find this up-to-date guide to HPLC’s recent developments essential for enhancing on-the-job technical expertise. Extensive coverage includes the broad applications of HPLC, ranging from major chromatographic techniques (including reversed phase, ion exchange, affinity and hydrophobic interaction chromatography) to specific separations such as those in monoclonal antibody and nucleic acid purification. Techniques for quality control programs and advanced technology are also discussed. 1990 (0 471-82584-0) 564 pp. Unified Separation Science J. Calvin Giddings This advanced text/monograph brings together for the first time the variety of techniques used for chemical separations by outlining their common underlying mechanisms. The mass transport phenomena underlying all separation processes are developed in a simple physical-mathematical form,facilitating analysis of alternative separation techniques and the factors integral to separation power. The first six chapters provide background material applicable to a wide range of separation methods, while the final five chapters illustrate specific techniques and methods.
ßçûê:
Ðóáðèêà: Òåõíîëîãèÿ /
Ñòàòóñ ïðåäìåòíîãî óêàçàòåëÿ: Ãîòîâ óêàçàòåëü ñ íîìåðàìè ñòðàíèö
ed2k: ed2k stats
Ãîä èçäàíèÿ: 1993
Êîëè÷åñòâî ñòðàíèö: 452
Äîáàâëåíà â êàòàëîã: 10.04.2009
Îïåðàöèè: Ïîëîæèòü íà ïîëêó |
Ñêîïèðîâàòü ññûëêó äëÿ ôîðóìà | Ñêîïèðîâàòü ID
Ïðåäìåòíûé óêàçàòåëü
Accuracy 227—229
Adsorption 18 96
Air quality 41
Alpha 6
Amino acid, analysis 37—40
Amino acid, sequencing 37—39
Analyte 18
Analytical chromatography 18
Application, focus 9
Application, gel permeation chromatography 16
Application, growth 11—18
Application, ion chromatography 16
Application, organic synthesis aid 51—60
Application, preparative chromatography 17
Application, shampoo 134
Application, typical uses of HPLC 13
ASTM, committee E-19 237
AUFS 18
Band 18
Band broadening 18 88—90 238—242
Bed 19
Bonded phase chromatography 19 97
Bonded phase chromatography, column preparation 211—213
Buffer 19 147—150 246—248
Capacity factor 19 85
Chromatogram 3 4 7 8 19 69 70
Chromatographic process 2—6
column 19
Column volume 19
Confidence limit 232
Correct values 235
Counter ion 19
databases 106 107
Decision tree for column choice 109
Degassing 249 313
Detector 19 81—85
Detector, high sensitivity 82
Detector, time constant 84
Detector, wide scope 82
Diagnostics 78—81
Differential Refractometer 19 181
Dry column chromatography 272
Efficieny 6 7 19 86—90 214—221
Efficieny, calculation 215 327
Efficieny, column length 346 350
Efficieny, comparison 220
Efficieny, flow rate 216 220
Eluent 19 see
Eluent strength see "Solvent strength"
Elute 20
Elutropic series 20 123 190
EPA methods for HPLC 45
Equieluotropic 138
Error curve 231
Error, constant 229
error, random 229 231
Essential oils, active ingredient 429
Essential oils, experiment 425—434
Estrogens 144—150
Exclusion chromatography see "Gel permeation chromatography"
FD&C dyes 330
Filter, in-line 71 74
Fittings 76—78
Flash chromatography 272
Flow programming 20 314—316
Flow rate 20 125 128 216—221
Flow rate, precision 236
Fronting 20 81
Gas chromatography 11
Gel filtration chromatography see "Gel permeation chromatography"
Gel permeation chromatography 20 46—51 99 112—114 177—186 359 366
Gel permeation chromatography, calibration curve 178—180
Gel permeation chromatography, column types 114
Gel permeation chromatography, efficiency 183
Gel permeation chromatography, exclusion limit 20
Gel permeation chromatography, experiment 358—369
Gel permeation chromatography, preparative capability 183—186
Gel permeation chromatography, small molecule 177—186 364 365 368
Gel permeation chromatography, solvent effects 180—182
Gene synthesis 30—33
Glossary 18—25
GPC see "Gel permeation chromatography"
Gradient elution 21 284—314
Gradient elution, applications 305—309
Gradient elution, considerations 310—314
Gradient elution, continuous 286
Gradient elution, experiment 435—447
Gradient elution, flow rate effect 301—305
Gradient elution, hardware contributions 268—293
Gradient elution, high-pressure mixing 289—292
Gradient elution, ideal system 288
Gradient elution, low-pressure mixing 289—292
Gradient elution, method development 293—305
Gradient elution, mixing devices 291
Gradient elution, nomenclature 286
Gradient elution, out-gassing 313
Gradient elution, precision 292 293
Gradient elution, pressure 312
Gradient elution, rate of change 294
Gradient elution, refractive index effects 310—313
Gradient elution, shapes 294
Gradient elution, step 286
Gradient elution, viscosity effects 310—313
Guard column 71 74
Height equivalent to a theoretical plate see "HETP"
Hemoglobin 34
HETP 21 87—90
Historical milestones 10 11
Human insulin 28 29
Injection 21
Injector 21 73
Instrumentation 2 3
Instrumentation, band spreading considerations 75—78
Instrumentation, diagnostics 78—81
Instrumentation, influence on performance parameters 79
Instrumentation, plumbing considerations 71—75
Interferon isolation 29
Ion exchange 21 98 122—124 167—176
Ion exchange, CM packing 173—175
Ion exchange, DEAE packing 173—175
Ion exchange, effect of 169 174
Ion exchange, effect of counterion 171
Ion exchange, effect of pH 169—171 174
Ion exchange, proteins 173—176
Ion exchange, temperature 171
Ion pair chromatography 21 22 98 157—164
Ion pair chromatography, retention models 161
Ion suppression 21 153—157 170
Ion-pair extraction 157
Isocratic, elution 21 285
Isocratic, system 70—75 319
Isoelectric point 174
Isoeluotrophic 21 138
k' see "Capacity factor"
Kinetics see "Reaction"
Linear velocity 21
Liquid-liquid chromatography 21
Liquid-liquid extraction 22
Liquid-solid chromatography 22 96
Matrix 22
Method development, databases 106 107
Method development, decision tree for column choice 109
Method development, general strategy 105—108
Method development, general tactics 108—110
Method development, next actions after first attempt 126 127
Method development, thin layer chromatography 192
Miscibility 244—246
Mobile phase 22 122—124
Mobile phase volume 22
Mobile phase, binary blend 139
Mobile phase, changing 246
Mobile phase, compatibility 249
Mobile phase, considerations for choosing 243—246 250
Mobile phase, nonaqueous 139—142
Mobile phase, preparation 254
Mobile phase, quality 251
Mobile phase, quaternary blend 139
Mobile phase, recirculation 242—243
Mobile phase, refractive index 312
Mobile phase, reservoir 72
Mobile phase, reverse phase 133—145
Mobile phase, selectivity 135—139
Mobile phase, strength 122 123
Mobile phase, tertiary blend 139
Mobile phase, viscosity 312
Molecular weight 46—51
Molecular weight distribution 46—51
N-nitroso compounds 42
NIOSH methods for HPLC 46
Normal phase 22 95—97 115 116 186—204 332 333
Normal phase, experiment 332—344
Normal phase, method development 191—202
Normal phase, mobile phase 189—191
Normal phase, stationary phases 187—189
Oligonucleotide isolation 30—33
OSHA methods for HPLC 47
Packing 22
Paired-ion chromatography see "Ion-pair chromatography"
Partition Chromatography see "Liquid-liquid chromatography"
Peak 22
Peak, area 22
Peak, height 22
Peak, maximum 22
Peak, width 23
Pellicular packing 23 90
Peptide, analysis 35
Peptide, mapping 36 37
Pesticide analysis 43—45
Plastics 46—49
Plate height see "HETP"
Plates 23 87—90
Polarity 23 122
Polarity of the molecule 191
Polarity, function group 193
Polyaromatic hydrocarbons 43 110—113
Polymer analysis 46—49
Porous packing 23
PRECISION 229—231
Precision, gradient analysis 293
Precision, LC analysis 237
Preparative chromatography 17 23 269—283 416 see
Preparative chromatography, approaching the problem 271
Preparative chromatography, classification 274
Preparative chromatography, collection 282
Preparative chromatography, detectors 280—282
Preparative chromatography, experiment 415—424
Preparative chromatography, method development 274—277
Preparative chromatography, monopropionamides of dicyanoheptamethylcobyrinate 53—60
Preparative chromatography, oligonucleotides 31—33
Preparative chromatography, recovery 282
Preparative chromatography, scale-up 188 277—280
Pressure read-out 78—81
Protein analysis 33—35 173—176
qualitative analysis 7 63—66
Quantitative analysis 7 385
Quantitative analysis, experiment 384—404
Reaction, hydrolysis of asprin experiment 405—414
Reaction, kinetics 406
Reaction, monitoring using HPLC 53—57 346
Recycle 23 221—227
Recycle, experiment 345—357
Recycle, shaving 228 346 353
Refractive index 81—83
Refractive index, comparison to UV 281 282
Refractive index, detector response 181 281
Resolution 6 7 23 92—94 328
Resolution, baseline 19
Resolution, influence of parameters upon individual terms 129
Resolution, peak purity 8
Resolution, relationship of alpha and plates 93
Retention, chromatography 23
Retention, equation 325
Retention, relation to phase polarity 116 121
Retention, relation to sample polarity 116
Retention, time 23
Retention, volume 23
Reverse phase 24 95 97 115—121 132—167 208—214 319 371
Reverse phase, column choice 213
Reverse phase, differences in columns 208—213
Reverse phase, effect of 153—155
Reverse phase, effect of pH 153—156
Reverse phase, experiment 318—331
Reverse phase, ionic compounds 151—167
Reverse phase, method development 133—139 142—167
Reverse phase, method development experiment 371—383
Reverse phase, nonaqueous 139—142
Reverse phase, silica gel 165—167
ri see "Refractive index"
Selectivity 6 86 94 135—139 327
Separation 2 85
Separation factor see "Alpha"
Separation, databases 106 107
Separation, decision tree for column choice 109
Separation, general strategies 105—108
Separation, general tactics 108—110
Separation, mechanisms 94—102
Significant figures 234 235
Silanols, contribution to retention 165
Silanols, types of 211
Size exclusion chromatography 24
Size separation see "Gel permeation chromatography"
Solid phase extraction 24 256—269
Solid phase extraction, conditioning 256
Solid phase extraction, effect of flow rate 262—267
Solid phase extraction, eluting 258
Solid phase extraction, examples 259—267
Solid phase extraction, guidelines 268 270
Solid phase extraction, isolating the analytes 258
Solid phase extraction, loading 257 267
Solid phase extraction, preparation of 264—267
Solid phase extraction, reconditioning 259
Solid phase extraction, recovery 269
Solute 24
Solvent 24
Solvent delivery system 24 72
Solvent programming see "Gradient elution"
Solvent, binary 139
Solvent, boiling point 245
Solvent, compatibility 249
Solvent, miscibility number 245
Solvent, physical properties 245
Solvent, polarity index 245
Solvent, quality 251
Solvent, quaternary 139
Solvent, strength 122 123 190 198 200
Solvent, tertiary 139
Solvent, viscosity 245
Solvent, water saturated 194 199
Sorption 24
Spices, major component 429
Spices, steam distillation 425 429
Standard deviation 230 395
Stationary phase 25 115 187
Statistics for chromatographers 224—238
Tailing 25 81 165
Therapeutic drug monitoring 60—63
Therapeutic drug monitoring, asthma 61 62
Therapeutic drug monitoring, epilepsy 62 63
Thin layer chromatography 192 272
Trace analysis 238—242
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