Cancer is the second leading cause of death globally. With the alarming increase in the rate of cancer incidence, there is a need for dedicated research and advanced diag- nostic measures. Cancer cell research has gained attention and has significantly impacted the development of potential therapeutics for different types of cancer. With the advancement in technology, cancer research has gained great momentum. The “Protocol handbook for Cancer Biology” is the collection of protocols/procedures used in studying cancer cell mechanism, drug discovery, diagnosis, and therapeutics. The chapters are contributed by the experts in the field comprising standardized protocols that will supplement research students and fel- lows involved in cancer research. Each chapter is in the form of a protocol for the detection of a specific characteristic feature of a cancer cell or tissue. There is ongoing research in cancer biology for several de- cades, and this book provides comprehen- sive standardized protocols along with alternative improvised techniques to the conventional ones. Each chapter contains complete information, starting with an introduction, equipment and reagents, pro- tocols, and statistical analysis of data. Furthermore, pros and cons, troubleshooting for the problems faced during these tech- niques, and alternative methods are also discussed.