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Название: Calcium Signaling Protocols
Автор: Lambert D.
It is extremely difficult to write a prescriptive account of how to measure
cytosolic-free Ca2+ ([Ca2+]c) that will suit all potential investigators, given the
wide diversity of fluorescent Ca2+ indicators that are now available, the variety
of cells to be investigated, and an increasing range of detection equipment that
can be used. Therefore, this chapter is designed to provide the user with sufficient
background in the technology so that he or she can move toward developing
a protocol that will suit the cells, the experimental objectives, and the
equipment available to the investigator.