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Название: Monoclonal Antibody Protocols
Автор: Davis W.
Аннотация:
Methods of Immunization to Enhance the Immune Response to Specific Antigens In Vivo in Preparation for Fusions Yielding Monoclonal Antibodies.
Methods of Immunization to Enhance the Immune Response to Specific Antigens In Vitro.
Culture Conditions that Optimize Outgrowth of Hybridomas.
Production of Stable Heterohybridomas Producing Human Monoclonal Antibodies.
Production of Stable Bovine-Murine Interspecies Hybrids.
Production of Monoclonal Antibodies in Swine.
Production of Monoclonal Antibodies in Horses.
Use of Electric-Field-Mediated Cell Fusion to Produce Hybridomas Secreting Monoclonal Antibodies.
Rapid Isotyping of Mouse Monoclonal Antibodies.
Methods of Measuring Antibodies in Sera/Plasma, Ascites, and Tissue-Culture Media.
Measurement of Immunoglobulin Synthesis Using the ELISPOT Assay.
Use of Monoclonal Antibodies in Immunohishemistry.
Use of Monoclonal Antibodies in Immunoelectron Microscopy.
Use of Monoclonal Antibodies for Western Blotting with Enhanced Chemiluminescent Detection.
The Use of Directly and Indirectly Labeled Monoclonal Antibodies in Flow Cytometry.
Use of Monoclonal Antibodies and Flow Cytometry to Cluster and Analyze Leukocyte Differentiation Molecules.
In Vivo and In Vitro Production of Monoclonal Antibodies: Bioreactors vs Immune Ascites.
Separation of Monoclonal Antibodies from Cell-Culture Supernatants and Ascites Fluid Using Thiophilic Agarose.
Detection, Purification, and Utilization of Murine Monoclonal IgM Antibodies.
Preparation and Use of Immunoaffinity Columns with Monoclonal Antibodies Without Purification from Ascites and Tissue-Culture Medium.
Antigen Purification by Monoclonal Antibody Immunoaffinity Chromatography.
Coupling of Monoclonal Antibodies with Fluorophores.
Coupling of Monoclonal Antibodies with Biotin.
Coupling of Monoclonal Antibodies with Enzymes.
Use of Monoclonal Antibodies with Magnetic Particles to Separate Cell Subpopulations by Negative Selection.
Use of Monoclonal Antibodies with Magnetic Particles to Separate Cell Subpopulations by Positive Selection.