The wealth of primary information provided by genome sequencing
projects in various species is of enormous potential value in our efforts to
understand biological funtions and molecular interactions not only in normal
development and cellular physiology, but also in diseases. However, utilization
of these resources can come only from the development and application
of a fully integrated set of molecular, biochemical, biophysical, and genetic
skill bases. As key components of many cell signalling pathways, protein
kinases are implicated in a broad variety of diseases, including cancers and
neurodegenerative conditions, and offer considerable potential as tractable
targets for therapeutic intervention. With these issues in mind, the present
vlume has been compiled to provide examples of core skills required for analysis
of knase-mediated signalling cascades, with particular emphasis on identification
of proteins according to interactive relationships and analysis of
functional properties of signalling proteins