The aim of The ELISA Guidebook is to expand the information concerning enzyme-linked
immunosorbent assay (ELISA) published in ELISA: Theory and Practice by J. R. Crowther (1995), in
the Methods in Molecular Biology series by Humana Press (vol. 42). The earlier book concentrated on
the immunological background of the reagents exploited in such assays, and dealt practically with the
various assays, through examples using noninfectious systems. This new volume is a major extension
and updating of that book, with a reorganization of the chapters, and extra information dealing, in
particular, with chessboard titration of reagents, quality control, monoclonal antibodies, validation of
assays, statistics, and epidemiological considerations. Suitable for scientists with previous experience of
the technique, it can, however, be used successfully by those with little experience, and as a teaching
aid.