This collection of cutting–edge techniques for the study of the eukaryotic cell cycle and its key regulatory molecules includes overviews of cell cycle regulatory mechanisms in many major research organisms. Described in step-by-step detail, these readily reproducible methods enable fundamental research on well-defined cell cycle regulators—and those more recently defined—in yeasts, bacteria, plants, Drosophila, Xenopus, and mammals. The book offers all cell researchers indispensable techniques essential to understanding how normal cells divide and how this is altered in disease.