The aim of Transgenic Plants: Methods and Protocols is to provide a source
of information to guide the reader through a wide range of frequently used,
broadly applicable, and easily reproducible techniques involved in the generation
of transgenic plants. Its step-by-step approach covers a series of methods
for genetically transforming plant cells and tissues, and for recovering whole
transgenic plants from them. The volume then moves on to the use of selectable
and reporter markers, positive selection, marker elimination after recovery
of transgenic plants, and the analysis of transgene integration, expression,
and localization in the plant genome. Although contributors usually refer to
model plants in most chapters, the protocols described herein should be widely
applicable to many plant species. The last two sections are devoted to methods
of risk assessment and to exploring the current and future applications of
transgenic technology in agriculture and its social implications in a case study.