We are proud to present the second edition of p53 protocols to keep up with the progress
in p53 research since the publication of its fi rst edition. The present edition includes 19
chapters delineating state-of-the art methods to address the challenging questions of the
p53 fi eld such as identi fi cation of the target genes and binding partners of gain of function
p53 mutants, methods to determine stress response, autophagy or senescence
induced by p53, cell cycle analysis, analysis of different phases of DNA replication modi fi ed
by p53, and generation of pluripotent stem cells.
As in the earlier version, the primary focus of p53 protocols was to include methods
that are important for research on p53 or other tumor suppressors. We have also attempted
to discuss the problems and to provide tips for troubleshooting. We owe thanks to Professor
John Walker for giving us the opportunity and encouragement to design and edit the second
edition. We also thank the staff of Humana Press for their help in compiling the book.
We sincerely thank all the contributing authors for sharing their invaluable experience to
the research community by writing the high-quality chapters. We would also like to thank
Dr. Rebecca Frum and our students Catherine Vaughan, Isabella Pearsall, Shilpa Singh, and
Olivia Bouton who enthusiastically acted as foot soldiers assisting us with grammatical
editing, communicating with the authors, and organizing the manuscript.