This book celebrates the importance of the question; it is not meant
to be a collection of facts or procedures. The writing of this book was
inspired by 16 years of queries from the research community.The contributors
and I have tried to meet two primary objectives:
• Enhance the reader’s ability to identify the critical elements of
any technique, reagent, or procedure, in order to address questions
for which documented answers might be unavailable.
• Clarify theory and practice that is taken for granted yet frequently
misapplied.