This book describes basic cell engineering methods, emphasizing stem cell applications, and use of the genetically modified stem cells in cell therapy and drug discovery. Together, the chapters introduce and offer insights on new techniques for engineering of stem cells and the delivery of transgenes into stem cells via various viral and non-viral systems. The book offers a guide to the types of manipulations currently available to create genetically engineered stem cells that suit any investigator's purpose, whether it's basic science investigation, creation of disease models and screens, or cells for therapeutic applications.Content:
Chapter 1 DNA Assembly Technologies Based on Homologous Recombination (pages 1–17): Billyana Tsvetanova, Lansha Peng, Xiquan Liang, Ke Li, Jian?Ping Yang, Tony Ho, Josh Shirley, Liewei Xu, Jason Potter, Wieslaw Kudlicki, Todd Peterson and Federico Katzen
Chapter 2 Multigene Assembly for Construction of Synthetic Operons: Creation and Delivery of an Optimized All?In?One Expression Construct for Generating Mouse iPS Cells (pages 19–36): Takefumi Sone, Yoko Takata, Hiroe Kishine, Taichi Andoh, Fumio Imamoto and Jonathan D. Chesnut
Chapter 3 Strategies for the Delivery of Naked DNA (pages 37–47): Jennifer C. Moore and Ronald P. Hart
Chapter 4 Episomal Vectors (pages 49–83): Junying Yu
Chapter 5 Nonintegrating DNA Virus (pages 85–102): Chris Kemp and David Kuninger
Chapter 6 Nonintegrating RNA Viruses (pages 103–118): Noemi Fusaki
Chapter 7 Protein Delivery (pages 119–133): Xiao?Jian Han and Kazuhito Tomizawa
Chapter 8 Sleeping Beauty Transposon?Mediated Stable Gene Delivery (pages 135–161): Andrew Wilber, Xianzheng Zhou, Xin Huang, Dan S. Kaufman and R. Scott McIvor
Chapter 9 Integrating Viral Vectors for Gene Modifications (pages 163–197): Gemma L. Mendel, Brian Paszkiet and Rafal P. Witek
Chapter 10 Bacteriophage Integrases for Site?Specific Integration (pages 199–209): Bhaskar Thyagarajan
Chapter 11 Improving Gene Targeting Efficiency in Human Pluripotent Stem Cells (pages 211–225): Sangyoon Han, Anna McCann, Louise C. Laurent, Jeanne F. Loring and Ying Liu
Chapter 12 Modified Stem Cells as Disease Models and in Toxicology Screening (pages 227–250): Catharina Ellerstrom, Peter Sartipy, Raimund Strehl and Johan Hyllner
Chapter 13 Screening and Drug Discovery (pages 251–272): Elizabeth A. Davenport and Robert S. Ames