Content: Gene silencing by RNA interference and the role of small interfering RNAs —
Basics of siRNA design and chemical synthesis —
Oligonucleotide scanning arrays in the design of small interfering RNAs —
siRNA production by in vitro transcription —
Production of siRNAs with the application of deoxyribozymes —
Production of siRNA in vitro by enzymatic digestion of double-stranded RNA —
Plasmid-mediated intracellular expression of siRNAs —
Lentiviral vector-mediated delivery of si/shRNA —
Exogenous siRNA delivery: protocols for optimizing delivery to cells —
RNAi in drosophila cell cultures —
RNAi in caenorhabditis elegans —
Delivery of RNAi reagents in C. elegans by microinjection —
RNAi in drosophila embryos —
RNAi in xenopus laevis oocytes —
RNAi in xenopus embryos —
An RNAi-based genomic library for forward genetics in the African trypanosome —
RNAi in the malaria parasite plasmodium —
Silencing the expression of cysteine proteases (falcipains) in plasmodium falciparum using RNA interference approaches —
RNAi in transgenic animal models —
RNA interference in mouse models —
Virus-induced RNA silencing in plants —
RNA silencing in plants: biolistic delivery of RNAi reagents —
Agro-infiltration: a versatile tool for RNAi studies in plants.