√лавна€    Ex Libris     ниги    ∆урналы    —татьи    —ерии     аталог    Wanted    «агрузка    ’удЋит    —правка    ѕоиск по индексам    ѕоиск    ‘орум   
blank
јвторизаци€

       
blank
ѕоиск по указател€м

blank
blank
blank
 расота
blank
Bontoux N., Dauphinot L., Potier M. Ч Unravelling single cell genomics : micro and nanotools
Bontoux N., Dauphinot L., Potier M. Ч Unravelling single cell genomics : micro and nanotools



ќбсудите книгу на научном форуме



Ќашли опечатку?
¬ыделите ее мышкой и нажмите Ctrl+Enter


Ќазвание: Unravelling single cell genomics : micro and nanotools

јвторы: Bontoux N., Dauphinot L., Potier M.

јннотаци€:

Content: Machine generated contents note: ch. 1 An Introduction to Molecular Biology / Luce Dauphinot Ч Abstract Ч 1.1. DNA Structure and Gene Expression Ч 1.2. Molecular Biology Tools for Nucleic Acid Studies Ч 1.2.1. DNA Engineering Ч 1.2.2. Polymerase Chain Reaction Ч 1.2.3. DNA Microarrays Ч References Ч ch. 2 The Central Dogma in Molecular Biology / Laili Mahmoudian Ч Abstract Ч 2.1. Replication Ч 2.2. Transcription Ч 2.3. Translation Ч 2.4. Regulation of Gene Expression Ч 2.4.1. Transcriptional Control Ч 2.4.2. Post-transcriptional Modifications Ч 2.4.3. Translational Control Ч 2.4.4. Post-translational Control Ч 2.5. Limitations of the Central Dogma Ч 2.6. Single Cells and their Complexity Ч References Ч ch. 3 From Unicellular to Multicellular Organisms: Tells from Evolution and from Development / Tania Vitalis Ч Abstract Ч 3.1. Cells from Evolution Ч 3.2. Cells from Development Ч References Ч ch. 4 Understanding Cellular Differentiation / Tania Vitalis Ч Abstract 4.1. Development of the Cerebral Cortex Ч 4.2. Neuronal Differentiation Ч 4.3. Single Cell Analysis in Differentiation Processes Ч References Ч ch. 5 Realistic Models of Neurons Require Quantitative Information at the Single-cell Level / Nicolas Le Novere Ч Abstract Ч 5.1. Introduction Ч 5.2. The Importance of Precise Neuronal Morphology Ч 5.3. Each Neuron has a Unique Neurochemistry Ч 5.4. Conclusions Ч References Ч ch. 6 Application to Cancerogenesis: Towards Targeted Cancer Therapies? / Christoph A. Klein Ч Abstract Ч 6.1. Molecular Diagnosis in Cancer Ч 6.2. Detection and Malignant Origin of Disseminated Cancer Cells Ч 6.3. Genomic Studies of Single Disseminated Cancer Cells Ч 6.4. Oncogene Dependence and Tumor Suppressor Sensitivity in Metastasis Founder Cells Ч References Ч ch. 7 Capturing a Single Cell / Joel Lachuer Ч Abstract Ч 7.1. Introduction Ч 7.2. Overview of Cell Sorting Technologies Ч 7.3. Laser Capture Microdissection Technologies Ч 7.3.1. Infrared Laser Capture Systems Ч 7.3.2. Ultraviolet Cutting Systems 7.4. Protocols Before Laser Microdissection (Tissue Sampling and Preparation) Ч 7.4.1. Dissection from Fresh Frozen Tissue Ч 7.4.2. Dissection from Formalin-fixed Paraffin-embedded Tissue Ч 7.4.3. Immuno Laser Capture Microdissection Ч 7.4.4. Other Cell-labeling Methods Ч 7.5. Conclusion Ч References Ч ch. 8 Looking at the DNA of a Single Cell / Christoph A. Klein Ч Abstract Ч 8.1. Challenges of Single Cell DNA Amplification Ч 8.2. Methods for Amplifying Genomic DNA of Single Cells Ч 8.3. Array Comparative Genomic Hybridization of Single Cells Ч 8.4. Combined Genome and Transcriptome Analysis of Single Cells Ч 8.5. Perspective on Single Cell DNA Analysis Ч References Ч ch. 9 Gene Analysis of Single Cells / Bertrand Lambolez Ч Abstract Ч 9.1. Single Cell RT-PCR After Patch Clamp Ч 9.2. Correlating mRNA Expression and Functional Properties of Single Cells Ч 9.3. Quantitative Analyses by scPCR Ч 9.4. Molecular and Functional Phenotyping of Neuronal Types Ч 9.5. Patch-clamp Harvesting of Single Cells 9.6. Sensitivity Limits Ч 9.7. Controls Ч 9.8. Interpretation of scPCR Results Ч Conclusion Ч Acknowledgement Ч References Ч ch. 10 Proteomics / Joelle Vinh Ч Abstract Ч 10.1. Motivation to Study Proteins at the Single Cell Level Ч 10.1.1. Proteins, mRNAs and DNA Ч 10.1.2. Sample Preparation Ч 10.1.3. Sub-proteome Analysis Ч 10.2. Analytical Strategies Ч 10.2.1. Mass Spectrometry Ч 10.2.2. Coupling Separation Techniques and Mass Spectrometry Ч 10.3. Strategies for Studying Proteins in Low Amounts of Samples Ч 10.3.1. How to Enhance the Sensitivity: Miniaturization, Integration, and Automation Ч 10.3.2. MALDI Interfaces Ч Conclusion Ч References Ч ch. 11 Microfluidics: Basic Concepts and Microchip Fabrication / Petra S. Dittrich Ч Abstract Ч 11.1. Size Matters: An Introduction Ч 11.2. A Short Chronology of Microfluidics Research Ч 11.3. Microfluidics: Some Basics Ч 11.3.1. Flow Generation Ч 11.3.2. Laminar Flow Ч 11.3.3. Digital Microfluidics: Segmented Flow Ч 11.4. Fabrication Techniques and Materials 11.4.1. Photolithography Ч 11.4.2. Soft Lithography Ч 11.4.3. Microchip Materials Ч 11.4.4. From Fabrication to Application Ч 11.5. Concluding Remarks Ч References Ч ch. 12 Cell Capture and Lysis on a Chip / Albert van den Berg Ч Abstract Ч 12.1. Introduction Ч 12.2. Cell Capture on a Chip Ч 12.2.1. Mechanical Trapping Ч 12.2.2. Electrical Trapping Ч 12.2.3. Fluidic Trapping Ч 12.2.4. Alternative Trapping Techniques Ч 12.2.5. Conclusion on Cell Trapping Ч 12.3. Cell Lysis in a Chip Ч 12.3.1. Thermal Lysis Ч 12.3.2. Chemical Lysis Ч 12.3.3. "Alkaline" or Electrochemical Lysis Ч 12.3.4. Electrical Lysis Ч 12.3.5. Mechanical Lysis Ч 12.3.6. Alternative Mechanical Lysis: Acoustic Lysis Ч 12.3.7. Optical Lysis Ч 12.3.8. Conclusion on Cell Lysis Ч 12.4. Conclusion Ч References Ч ch. 13 DNA Analysis in Microfluidic Devices and their Application to Single Cell Analysis / Angelique Le Bras Ч Abstract Ч 13.1. Amplification on a Chip Ч 13.1.1. Polymerase Chain Reaction Ч 13.1.2. Isothermal Techniques 13.2. DNA Analysis Ч 13.2.1. Real-time PCR Detection Ч 13.2.2. Capillary Electrophoresis Ч 13.3. Why and When Smaller is Better Ч 13.4. Applications of Microfluidic Single Cell Genetic Analysis in Microbial Ecology Ч 13.5. Conclusion Ч References Ч ch. 14 Gene Expression Analysis on Microchips / Max Chahert Ч Abstract Ч 14.1. Introduction Ч 14.2. Multi-step Microfluidic RT-PCR Ч 14.3. One-step Microfluidic RNA Analysis Ч 14.4. Microfluidic cDNA Analysis Ч 14.5. Single Cell RNA Analysis Ч 14.6. Conclusion Ч Acknowledgement Ч References Ч ch. 15 Analysis of Proteins at the Single Cell Level / Severine Le Gac Ч Abstract Ч 15.1. Introduction Ч 15.1.1. Protein Analysis: The Challenge Ч 15.1.2. Why Microfluidics? Ч 15.1.3. Microfluidics and Protein Analysis Ч 15.2. Electrospray Ionization Mass Spectrometry Ч 15.2.1. Connections and Coupling Ч 15.2.2. Sample Processing: Purification and Digestion Ч 15.2.3. Integrated Systems Ч 15.3. MALDI-MS Ч 15.3.1. Microfabricated MALDI Targets 15.3.2. Off-line Sample Preparation Ч 15.3.3. Integrated Microsystems Ч 15.4. Innovative Approaches for Protein Analysis at the Single Cell Level Ч 15.4.1. Invasive Analysis Ч 15.4.2. Partially Invasive Analysis Ч 15.4.3. Non-invasive Analysis Ч 15.5. Conclusion and Perspectives Ч References Ч ch. 16 A Concrete Case: A Microfluidic Device for Single Cell Whole Transcriptome Analysis / Marie-Claude Potier Ч Abstract Ч 16.1. Introduction Ч 16.2. Choice of Biological Protocol, Material and Fabrication Technique Ч 16.2.1. Protocols for Single Cell Whole Transcriptome Analysis Ч 16.2.2. Miniaturizing Reactions: Continuous Flows, Reaction Chambers or Droplet Micro-fluidic Reactions Ч 16.2.3. Choosing the Microchip Material Ч 16.2.4. Microchip Fabrication Ч 16.3. Integrating Reverse Transcription on a Chip Ч 16.3.1. Gene Expression Profiling of Single-Cell Scale Amounts of RNA Ч 16.3.2. Gene Expression Profiling of Single Cells Ч 16.4. Amplifying the Transcriptome on a Chip Ч 16.5. Detecting the Transcriptome on a Chip 16.5.1. Microfluidics and Conventional Microarrays Ч 16.5.2. Microarray Development Using DNA Immobilization onto Microchannels Ч 16.5.3. Towards Transcriptome Analysis in the Liquid Phase Ч 16.6. Some Practical Conclusions Ч References Ч ch. 17 Tiny Droplets for High-throughput Cell-based Assays / V. Taly Ч Abstract Ч 17.1. Introduction Ч 17.2. Droplet-based Microfluidics Ч 17.2.1. EWOD and "Digital Microfluidics": Tools for High-content Screening Ч 17.2.2. Droplet-based Microfluidics: Tools for High-throughput Screening Ч 17.3. Generating and Manipulating Droplets Ч 17.3.1. Droplet Production Ч 17.3.2. Droplet Division Ч 17.3.3. Droplet Flow, Droplet Synchronization, and Droplet Incubation Ч 17.3.4. Droplet Content Detection and Droplet Sorting Ч 17.4. In Vitro Compartmentalization of Biological Reactions Ч 17.4.1. Cell Compartmentalization in Aqueous Droplets Ч 17.4.2. Incubation and Cell Viability in Droplets Ч 17.4.3. Cell-based Assays and Cell Manipulation Ч 17.5. Towards Integrated Platforms for Cell-based Assays 17.6. Conclusions Ч References Ч ch. 18 New Detection Methods for Single Cells / Emmanuel Fort Ч Abstract Ч 18.1. Introduction Ч 18.2. Bio-barcode Strategy Ч 18.2.1. Principle Ч 18.2.2. An Example: DNA Origami Ч 18.3. Imaging Gene Expression in Living Cells Ч 18.3.1. Motivations Ч 18.3.2. Improvements in Photonic Microscopy Ч 18.3.3. Improvements in Fluorophore Design Ч 18.4. Quantum Dots-based Techniques Ч 18.4.1. Quantum Dots Bead-based Assays Ч 18.4.2. Single Quantum Dots-based DNA Nanosensors Ч 18.4.3. Quantum Dots for Super-resolution Microscopy Ч 18.5. Gold Nanoparticle-based Detection Methods Ч 18.5.1. Resonant Light Scattering Detection Ч 18.5.2. Molecular Beacons with Gold Nanoparticles Ч 18.5.3. Molecular Plasmonic Rulers Ч 18.5.4. Surface-enhanced Raman Scattering Detection Ч 18.6. Electrochemical Sensors Ч 18.7. Concluding Remarks Ч References


язык: en

–убрика: –азное/

—татус предметного указател€: Ќеизвестно

ed2k: ed2k stats

√од издани€: 2010

 оличество страниц: 318

ƒобавлена в каталог: 15.04.2017

ќперации: ѕоложить на полку | —копировать ссылку дл€ форума | —копировать ID
blank
ѕредметный указатель
blank
–еклама
blank
blank
HR
@Mail.ru
       © Ёлектронна€ библиотека попечительского совета мехмата ћ√”, 2004-2021
Ёлектронна€ библиотека мехмата ћ√” | Valid HTML 4.01! | Valid CSS! ќ проекте