Explores new applications emerging from our latest understanding of proteins in solution and at interfaces

Proteins in solution and at interfaces increasingly serve as the starting point for exciting new applications, from biomimetic materials to nanoparticle patterning. This book surveys the state of the science in the field, offering investigators a current understanding of the characteristics of proteins in solution and at interfaces as well as the techniques used to study these characteristics. Moreover, the authors explore many of the new and emerging applications that have resulted from the most recent studies. Topics include protein and protein aggregate structure; computational and experimental techniques to study protein structure, aggregation, and adsorption; proteins in non-standard conditions; and applications in biotechnology.

Proteins in Solution and at Interfaces is divided into two parts:

• Part One introduces concepts as well as theoretical and experimental techniques that are used to study protein systems, including X-ray crystallography, nuclear magnetic resonance, small angle scattering, and spectroscopic methods
• Part Two examines current and emerging applications, including nanomaterials, natural fibrous proteins, and biomolecular thermodynamics

The book's twenty-three chapters have been contributed by leading experts in the field. These contributions are based on a thorough review of the latest peer-reviewed findings as well as the authors' own research experience. Chapters begin with a discussion of core concepts and then gradually build in complexity, concluding with a forecast of future developments.

Readers will not only gain a current understanding of proteins in solution and at interfaces, but also will discover how theoretical and technical developments in the field can be translated into new applications in material design, genetic engineering, personalized medicine, drug delivery, biosensors, and biotechnology.Content:
Chapter 1 X?Ray Crystallography of Biological Macromolecules: Fundamentals and Applications (pages 1–22): Antonio L. Llamas?Saiz and Mark J. van Raaij
Chapter 2 Nuclear Magnetic Resonance Methods for Studying Soluble, Fibrous, and Membrane?Embedded Proteins (pages 23–48): Victoria A. Higman
Chapter 3 Small?Angle X?Ray Scattering Applied to Proteins in Solution (pages 49–72): Leandro Ramos Souza Barbosa, Francesco Spinozzi, Paolo Mariani and Rosangela Itri
Chapter 4 Analyzing the Solution State of Protein Structure, Interactions, and Ligands by Spectroscopic Methods (pages 73–98): Veronica I. Dodero and Paula V. Messina
Chapter 5 Resolving Membrane?Bound Protein Orientation and Conformation by Neutron Reflectivity (pages 99–111): Hirsh Nanda
Chapter 6 Investigating Protein Interactions at Solid Surfaces—In Situ, Nonlabeling Techniques (pages 113–138): Olof Svensson, Javier Sotres and Alejandro Barrantes
Chapter 7 Calorimetric Methods to Characterize the Forces Driving Macromolecular Association and Folding Processes (pages 139–177): Conceicao A.S.A. Minetti, Peter L. Privalov and David P. Remeta
Chapter 8 Virtual Ligand Screening Against Comparative Models of Proteins (pages 179–191): Hao Fan
Chapter 9 Atomistic and Coarse?Grained Molecular Dynamics Simulations of Membrane Proteins (pages 193–206): Thomas J. Piggot, Peter J. Bond and Syma Khalid
Chapter 10 Preparation of Nanomaterials Based on Peptides and Proteins (pages 207–218): Yujing Sun and Zhuang Li
Chapter 11 Natural Fibrous Proteins: Structural Analysis, Assembly, and Applications (pages 219–232): Mark J. van Raaij and Anna Mitraki
Chapter 12 Amyloid?Like Fibrils: Origin, Structure, Properties, and Potential Technological Applications (pages 233–282): Pablo Taboada, Silvia Barbosa, Josue Juarez, Manuel?Alatorre Meda and Victor Mosquera
Chapter 13 Proteins and Peptides in Biomimetic Polymeric Membranes (pages 283–290): Alfredo Gonzalez?Perez
Chapter 14 Study of Proteins and Peptides at Interfaces by Molecular Dynamics Simulation Techniques (pages 291–313): David Poger and Alan E. Mark
Chapter 15 A Single?Molecule Approach to Explore the Role of the Solvent Environment in Protein Folding (pages 315–334): Katarzyna Tych and Lorna Dougan
Chapter 16 Enhanced Functionality of Peroxidases by Its Immobilization at the Solid–Liquid Interface of Mesoporous Materials and Nanoparticles (pages 335–351): Jose Campos?Teran, Iker Inarritu, Jorge Aburto and Eduardo Torres
Chapter 17 Superactivity of Enzymes in Supramolecular Hydrogels (pages 353–364): Ye Zhang and Bing Xu
Chapter 18 Surfactant Proteins and Natural Biofoams (pages 365–377): Malcolm W. Kennedy and Alan Cooper
Chapter 19 Promiscuous Enzymes (pages 379–387): Luis F. Olguin
Chapter 20 Thermodynamics and Kinetics of Mixed Protein/Surfactant Adsorption Layers at Liquid Interfaces (pages 389–427): Reinhard Miller, E.V. Aksenenko, V.S. Alahverdjieva, V.B. Fainerman, C.S. Kotsmar, J. Kragel, M.E. Leser, J. Maldonado?Valderrama, V. Pradines, C. Stefaniu, A. Stocco and R. Wustneck
Chapter 21 Application of Force Spectroscopy Methods to the Study of Biomaterials (pages 429–435): Chuan Xu and Erika F. Merschrod S.
Chapter 22 Protein Gel Rheology (pages 437–448): Katie Weigandt and Danilo Pozzo
Chapter 23 Exploring Biomolecular Thermodynamics in Aqueous and Nonaqueous Environments Using Time?Resolved Photothermal Methods (pages 449–472): Randy W. Larsen, Carissa M. Vetromile, William A. Maza, Khoa Pham and Jaroslava Miksovska