Plant Transformation Technologies is a comprehensive, authoritative book focusing on cutting-edge plant biotechnologies, offering in-depth, forward-looking information on methods for controlled and accurate genetic engineering. In response to ever-increasing pressure for precise and efficient integration of transgenes in plants, many new technologies have been developed. With complete coverage of these technologies,
Plant Transformation Technologies provides valuable insight on current and future plant transformation technologies.
With twenty-five chapters written by international experts on transformation technologies, the book includes new information on Agrobacterium, targeting transgenes into plant genomes, and new vectors and market systems. Including both review chapters and protocols for transformation, Plant Transformation Technologies is vitally important to graduate students, postdoctoral students, and university and industry researchers.
Content:
Chapter 1 Host Factors Involved in Genetic Transformation of Plant Cells by Agrobacterium (pages 1–29): Benoit Lacroix, Adi Zaltsman and Vitaly Citovsky
Chapter 2 Genomics of Agrobacterium–Plant Interaction: An Approach to Refine the Plant Transformation Technology (pages 31–49): Ajith Anand, Zarir E. Vaghchhipawala and Kirankumar S. Mysore
Chapter 3 Particle Bombardment: An Established Weapon in the Arsenal of Plant Biotechnologists (pages 51–71): Theodore M. Klein
Chapter 4 A Novel Gene Delivery System in Plants with Calcium Alginate Micro?Beads (pages 73–81): Naoki Wada, Shin'ichiro Kajiyama, Naruemon Khemkladngoen and Kiichi Fukui
Chapter 5 Pollen Transformation Technologies (pages 83–91): Tatiana Resch and Alisher Touraev
Chapter 6 Intragenic Vectors and Marker?Free Transformation: Tools for a Greener Biotechnology (pages 93–107): Caius M. Rommens, Anthony Conner, Hua Yan and Zac Hanley
Chapter 7 Visualizing Transgene Expression (pages 109–119): John J. Finer
Chapter 8 Current State and Perspective of Binary Vectors and Superbinary Vectors (pages 121–138): Toshiyuki Komori and Toshihiko Komari
Chapter 9 Novel Dual Binary Vectors (pCLEAN) for Plant Transformation (pages 139–147): Philippe Vain
Chapter 10 pORE Modular Vectors for Plant Transformation (pages 149–159): Dwayne D. Hegedus
Chapter 11 pANIC: A Versatile Set of Gateway?Compatible Vectors for Gene Overexpression and RNAi?Mediated down?Regulation in Monocots (pages 161–168): David G. J. Mann, Peter R. LaFayette, Laura L. Abercrombie, Wayne A. Parrott and C. Neal Stewart
Chapter 12 Understanding and Avoiding Transgene Silencing (pages 169–196): Michael W. Graham, Stephen R. Mudge, Peter R. Sternes and Robert G. Birch
Chapter 13 Site?Specific Recombination for Precise and “Clean” Transgene Integration in Plant Genome (pages 197–209): Vibha Srivastava and David W. Ow
Chapter 14 Selectable Marker Genes: Types and Interactions (pages 211–228): Brian Miki, Ashraf Abdeen, Yuzuki Manabe, Souad El Ouakfaoui, Douglas A. Johnson, Loreta Gudynaite?Savitch, Ming Hu and Phil MacDonald
Chapter 15 Transformation Methods for Obtaining Marker?Free Genetically Modified Plants (pages 229–242): Jan G. Schaart, Frans A. Krens, Anne?Marie A. Wolters and Richard G. F. Visser
Chapter 16 Intellectual Property Aspects of Plant Transformation (pages 243–270): Jim M. Dunwell