NMR is one of the most powerful methods for imaging of biomolecules. This book is the ultimate NMR guide for researchers in the biomedical community and gives not only background and practical tips but also a forward looking view on the future of NMR in systems biology.Content:
Chapter 1 NMR and its Place in Mechanistic Systems Biology (pages 1–5): Prof. Dr. Ivano Bertini, Kathleen S. McGreevy and Prof. Giacomo Parigi
Chapter 2 Structure of Biomolecules: Fundamentals (pages 7–32): Lucia Banci, Francesca Cantini, Mirko Cevec, Hendrik R. A. Jonker, Senada Nozinovic, Christian Richter and Harald Schwalbe
Chapter 3 What Can be Learned about the Structure and Dynamics of Biomolecules from NMR (pages 33–50): Lucio Ferella, Antonio Rosato, Paola Turano and Janez Plavec
Chapter 4 Determination of Protein Structure and Dynamics (pages 51–94): Lucio Ferella, Antonio Rosato and Paola Turano
Chapter 5 DNA (pages 96–116): Janez Plavec
Chapter 6 RNA (pages 118–135): Richard Stefl and Vladimir Sklenar
Chapter 7 Intrinsically Disordered Proteins (pages 136–152): Isabella C. Felli, Roberta Pierattelli and Peter Tompa
Chapter 8 Paramagnetic Molecules (pages 154–171): Ivano Bertini, Claudio Luchinat and Giacomo Parigi
Chapter 9 NMR Methodologies for the Analysis of Protein–Protein Interactions (pages 173–194): Tobias Madl and Michael Sattler
Chapter 10 Metal?Mediated Interactions (pages 196–203): Simone Ciofi?Baffoni
Chapter 11 Protein–Paramagnetic Protein Interactions (pages 204–217): Peter H. J. Keizers, Yoshitaka Hiruma and Marcellus Ubbink
Chapter 12 Protein–RNA Interactions (pages 218–236): Vijayalaxmi Manoharan, Jose Manuel Perez?Canadillas and Andres Ramos
Chapter 13 Protein–DNA Interactions (pages 238–252): Lidija Kovacic and Rolf Boelens
Chapter 14 High?Throughput Screening and Fragment?Based Design: General Considerations for Lead Discovery and Optimization (pages 253–263): Maurizio Pellecchia
Chapter 15 Ligand?Observed NMR in Fragment?Based Approaches (pages 264–280): Pawel Sledz, Chris Abell and Alessio Ciulli
Chapter 16 Interactions of Metallodrugs with DNA (pages 282–296): Hong?Ke Liu and Peter J. Sadler
Chapter 17 RNA as a Drug Target (pages 298–313): Jan?Peter Ferner, Elke Duchardt Ferner, Jorg Rinnenthal, Janina Buck, Jens Wohnert and Harald Schwalbe
Chapter 18 Fluorine NMR Spectroscopy for Biochemical Screening in Drug Discovery (pages 314–327): Claudio Dalvit
Chapter 19 NMR of Peptides (pages 328–344): Johannes G. Beck, Andreas O. Frank and Horst Kessler
Chapter 20 Biomolecular Solid?State NMR/Basics (pages 345–364): Emeline Barbet?Massin and Guido Pintacuda
Chapter 21 Protein Dynamics in the Solid State (pages 366–375): Jozef R. Lewandowski and Lyndon Emsley
Chapter 22 Microcrystalline Proteins – An Ideal Benchmark for Methodology Development (pages 376–392): W. Trent Franks, Barth?Jan van Rossum, Benjamin Bardiaux, Enrico Ravera, Giacomo Parigi, Claudio Luchinat and Hartmut Oschkinat
Chapter 23 Structural Studies of Protein Fibrils by Solid?State NMR (pages 394–405): Anja Bockmann and Beat H. Meier
Chapter 24 Solid?State NMR on Membrane Proteins: Methods and Applications (pages 406–417): A. A. Cukkemane, M. Renault and M. Baldus
Chapter 25 Dynamic Nuclear Polarization (pages 419–431): Thomas F. Prisner
Chapter 26 13C Direct Detection NMR (pages 432–443): Isabella C. Felli and Roberta Pierattelli
Chapter 27 Speeding up Multidimensional NMR Data Acquisition (pages 444–465): Bernhard Brutscher, Dominique Marion and Lucio Frydman
Chapter 28 Metabolomics (pages 466–477): Leonardo Tenori
Chapter 29 In?Cell Protein NMR Spectroscopy (pages 478–494): David S. Burz, David Cowburn, Kaushik Dutta and Alexander Shekhtman
Chapter 30 Structural Investigation of Cell?Free Expressed Membrane Proteins (pages 496–508): Solmaz Sobhanifar, Sina Reckel, Frank Lohr, Frank Bernhard and Volker Dotsch
Chapter 31 Grid Computing (pages 509–518): Antonio Rosato
Chapter 32 Protein–Protein Docking with HADDOCK (pages 520–535): Christophe Schmitz, Adrien S. J. Melquiond, Sjoerd J. de Vries, Ezgi Karaca, Marc van Dijk, Panagiotis L. Kastritis and Alexandre M. J. J. Bonvin
Chapter 33 Automated Protein Structure Determination Methods (pages 536–546): Paul Guerry and Torsten Herrmann
Chapter 34 NMR Structure Determination of Protein–Ligand Complexes (pages 548–561): Ulrich Schieborr, Sridhar Sreeramulu and Harald Schwalbe
Chapter 35 Small Angle X?Ray Scattering/Small Angle Neutron Scattering as Methods Complementary to NMR (pages 562–574): M. V. Petoukhov and D. I. Svergun