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Название: Protocols in Molecular Neurobiology
Авторы: Longstaff A., Revest P.
Аннотация:
Contents
Separation of Large DNA Molecules by Pulsed-Field Gel Electrophoresis.
The Isolation of Genomic DNA from Invertebrates.
Analysis of Mihondrial DNA Mutations.
The Design and Use of Oligonucleotides.
The Use of Degenerate Oligonucleotides for Polymerase Chain-Reaction-Based Isolation of Related DNA Sequences.
Isolation of Photoreceptor Cell-Specific MEKA cDNA by Differential Hybridization.
Generation of Isoform-Specific Antisera from Cloned cDNAs: Application to Multiple Forms of the Na/K-ATPase Expressed in Rat Brain.
Determination of Transcriptional Initiation Sites and Their Usage in the Nervous System.
Mapping of Trans-Acting Factor Binding in the Nervous System.
In Situ Hybridization to Brain Tissue Sections Using Labeled Single-Strand Complementary RNA Probes.
In Situ Hybridization Hishemistry Using Alkaline Phosphatase-Labeled Oligodeoxynucleotide Probe.
The Identification of Neuropeptide Gene Regulatory Elements in Transgenic Mice.
Expression of Exogenous Ion Channels and Neurotransmitter Receptors in RNA-Injected Xenopus Oocytes.
Analysis of Insulin and Insulin-Like Growth Factor-I Receptors in Neural Tissues.
Use of Affinity Chromatography in Purification of A1 Adenosine Receptors from Rat Brain Membranes.
Purification and Structure of L-Type Calcium Channels.
Purification and Reconstitution of the Ryanodine-Sensitive Ca2+ Release Channel Complex from Muscle Sarcoplasmic Reticulum.
Identification of a Ligand-Gated Ion Channel by Photoaffinity Labeling and Microsequencing. Voltage-Gated Ion Channels: Electrophysiological Approaches.
An Electrophysiological Approach to the Regulation of Neuronal Voltage-Activated Calcium Channels by Guanine Nucleotide Binding Proteins.