Preface
Contributors
Preparing for Success in the Laboratory
Getting What You Need from a Supplier
The Preparation of Buffers and Other Solutions: A Chemist’s Perspective
How to Properly Use and Maintain Laboratory Equipment
Working Safely with Biological Samples
Working Safely with Radioactive Materials
DNA Purification
RNA Purification
Restriction Endonucleases
Nucleotides, Oligonucleotides, and Polynucleotides
PCR
Electrophoresis
Western Blotting
Nucleic Acid Hybridization
E. coli Expression Systems
Eukaryotic Expression
Index