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Walker J. — New Protein Techniques
Walker J. — New Protein Techniques

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Название: New Protein Techniques

Автор: Walker J.

Аннотация:

Contents
Prevention of Unwanted Proteolysis
The Bradford Method for Protein Quantitation
Amino Acid Analysis by Precolumn Derivatization
Identification of N-Terminal Amino Acids by High-Performance Liquid Chromatography
Enzymatic Methods for Cleaving Proteins
Chemical Cleavage of Proteins
Chemical Modification of Proteins
The Design, Preparation, and Use of Immunopurification Reagents
Dye-Ligand Chromatography
Aminohexyl-Sepharose Affinity Chromatography
Purification of DNA-Dependent RNA Polymerase from Eubacteria
Direct Immunoprecipitation of Protein
Detection of Proteins in Polyacrylamide Gels Using an Ultrasensitive Silver Staining Technique
Chromatofocusing
Hybrid Isoelectric Focusing Using Mixed Synthetic-Carrier Ampholyte-Immobilized pH Gradient Gels
Two-Dimensional Electrophoresis Using Immobilized pH Gradients in the First Dimension
Two-Dimensional Polyacrylamide Gel Electrophoresis Using Flat-Bed Isoelectric Focusing in the First Dimension
Preparative Aspects of Immobilized pH Gradients
Isoelectric Focusing Under Denaturing Conditions
Computer Analysis of 2-D Electrophoresis Gels
Peptide Synthesis
Synthesis of a Series of Analogous Peptides Using T-Bags
Production of Antisera to Synthetic Peptides
Production of Antibodies Using Proteins in Gel Bands
Purification of Immunoglobulins Using Protein A-Sepharose
Antibody-Enzyme Conjugate Formation
Vacuum Blotting
Blotting with Plate Electrodes
Use of Dried Milk for Immunoblotting
Immunodetection of Proteins on Western Blots Using 125I-Labeled Protein A
Detection of Protein Blots Using the Avidin-Biotin System
Detection of Protein Blots Using Enzyme-Linked Second Antibodies or Protein A
Colloidal Gold for the Detection of Proteins on Blots and Immunoblots
Enzyme Immobilization by Adsorption
Look Inside Get Access
Enzyme Immobilization by Entrapment
Enzyme Immobilization by Covalent Bonding
Cell Immobilization


Язык: en

Рубрика: Разное/

Статус предметного указателя: Неизвестно

ed2k: ed2k stats

Год издания: 1988

Количество страниц: 524

Добавлена в каталог: 21.05.2016

Операции: Положить на полку | Скопировать ссылку для форума | Скопировать ID
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