Contents
Prevention of Unwanted Proteolysis
The Bradford Method for Protein Quantitation
Amino Acid Analysis by Precolumn Derivatization
Identification of N-Terminal Amino Acids by High-Performance Liquid Chromatography
Enzymatic Methods for Cleaving Proteins
Chemical Cleavage of Proteins
Chemical Modification of Proteins
The Design, Preparation, and Use of Immunopurification Reagents
Dye-Ligand Chromatography
Aminohexyl-Sepharose Affinity Chromatography
Purification of DNA-Dependent RNA Polymerase from Eubacteria
Direct Immunoprecipitation of Protein
Detection of Proteins in Polyacrylamide Gels Using an Ultrasensitive Silver Staining Technique
Chromatofocusing
Hybrid Isoelectric Focusing Using Mixed Synthetic-Carrier Ampholyte-Immobilized pH Gradient Gels
Two-Dimensional Electrophoresis Using Immobilized pH Gradients in the First Dimension
Two-Dimensional Polyacrylamide Gel Electrophoresis Using Flat-Bed Isoelectric Focusing in the First Dimension
Preparative Aspects of Immobilized pH Gradients
Isoelectric Focusing Under Denaturing Conditions
Computer Analysis of 2-D Electrophoresis Gels
Peptide Synthesis
Synthesis of a Series of Analogous Peptides Using T-Bags
Production of Antisera to Synthetic Peptides
Production of Antibodies Using Proteins in Gel Bands
Purification of Immunoglobulins Using Protein A-Sepharose
Antibody-Enzyme Conjugate Formation
Vacuum Blotting
Blotting with Plate Electrodes
Use of Dried Milk for Immunoblotting
Immunodetection of Proteins on Western Blots Using 125I-Labeled Protein A
Detection of Protein Blots Using the Avidin-Biotin System
Detection of Protein Blots Using Enzyme-Linked Second Antibodies or Protein A
Colloidal Gold for the Detection of Proteins on Blots and Immunoblots
Enzyme Immobilization by Adsorption
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Enzyme Immobilization by Entrapment
Enzyme Immobilization by Covalent Bonding
Cell Immobilization